Restriction map for
pFN21AB5261
(Linear) (Six-base) MAPSORT of: KIBB5261.seq Check: 4181 from: 1 to: 606
KIBB5261.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 511 606
Size: 511 95
AccI GT'mk_AC
Cuts at: 0 407 464 606
Size: 407 57 142
Fragments arranged by size:
407 142 57
Acc65I G'GTAC_C
Cuts at: 0 311 320 606
Size: 311 9 286
Fragments arranged by size:
311 286 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 606
Size: 350 256
ApaI G_GGCC'C
Cuts at: 0 583 606
Size: 583 23
AvaI C'yCGr_G
Cuts at: 0 54 511 606
Size: 54 457 95
Fragments arranged by size:
457 95 54
BanI G'GyrC_C
Cuts at: 0 49 68 131 311 320 562 606
Size: 49 19 63 180 9 242 44
Fragments arranged by size:
242 180 63 49 44 19 9
BanII G_rGCy'C
Cuts at: 0 583 606
Size: 583 23
BbeI G_GCGC'C
Cuts at: 0 53 72 135 566 606
Size: 53 19 63 431 40
Fragments arranged by size:
431 63 53 40 19
BglI GCCn_nnn'nGGC
Cuts at: 0 58 606
Size: 58 548
Bme1580I G_kGCm'C
Cuts at: 0 583 606
Size: 583 23
BmgBI CAC'GTC
Cuts at: 0 384 606
Size: 384 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 528 606
Size: 136 392 78
Fragments arranged by size:
392 136 78
BsaHI Gr'CG_yC
Cuts at: 0 50 69 132 563 606
Size: 50 19 63 431 43
Fragments arranged by size:
431 63 50 43 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 277 307 606
Size: 109 30 138 30 299
Fragments arranged by size:
299 138 109 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 391 606
Size: 121 270 215
Fragments arranged by size:
270 215 121
BseYI C'CCAG_C
Cuts at: 0 38 177 576 606
Size: 38 139 399 30
Fragments arranged by size:
399 139 38 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 606
Size: 483 123
BsiEI CG_ry'CG
Cuts at: 0 82 103 143 237 250 456 606
Size: 82 21 40 94 13 206 150
Fragments arranged by size:
206 150 94 82 40 21 13
BsiHKAI G_wGCw'C
Cuts at: 0 152 606
Size: 152 454
Bsp1286I G_dGCh'C
Cuts at: 0 152 583 606
Size: 152 431 23
Fragments arranged by size:
431 152 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 332 511 606
Size: 269 63 179 95
Fragments arranged by size:
269 179 95 63
BsrFI r'CCGG_y
Cuts at: 0 10 98 142 448 606
Size: 10 88 44 306 158
Fragments arranged by size:
306 158 88 44 10
BssHII G'CGCG_C
Cuts at: 0 196 606
Size: 196 410
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 606
Size: 492 114
BtgI C'CryG_G
Cuts at: 0 44 82 491 606
Size: 44 38 409 115
Fragments arranged by size:
409 115 44 38
EaeI y'GGCC_r
Cuts at: 0 79 100 140 247 453 606
Size: 79 21 40 107 206 153
Fragments arranged by size:
206 153 107 79 40 21
EagI C'GGCC_G
Cuts at: 0 79 100 140 247 453 606
Size: 79 21 40 107 206 153
Fragments arranged by size:
206 153 107 79 40 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 350 528 606
Size: 136 214 178 78
Fragments arranged by size:
214 178 136 78
EcoO109I rG'GnC_Cy
Cuts at: 0 580 606
Size: 580 26
FspI TGC'GCA
Cuts at: 0 265 606
Size: 265 341
HaeII r_GCGC'y
Cuts at: 0 28 53 72 135 401 566 606
Size: 28 25 19 63 266 165 40
Fragments arranged by size:
266 165 63 40 28 25 19
KasI G'GCGC_C
Cuts at: 0 49 68 131 562 606
Size: 49 19 63 431 44
Fragments arranged by size:
431 63 49 44 19
KpnI G_GTAC'C
Cuts at: 0 315 324 606
Size: 315 9 282
Fragments arranged by size:
315 282 9
MslI CAynn'nnrTG
Cuts at: 0 490 606
Size: 490 116
MspA1I CmG'CkG
Cuts at: 0 46 84 123 177 598 606
Size: 46 38 39 54 421 8
Fragments arranged by size:
421 54 46 39 38 8
NaeI GCC'GGC
Cuts at: 0 12 144 450 606
Size: 12 132 306 156
Fragments arranged by size:
306 156 132 12
NarI GG'CG_CC
Cuts at: 0 50 69 132 563 606
Size: 50 19 63 431 43
Fragments arranged by size:
431 63 50 43 19
NgoMIV G'CCGG_C
Cuts at: 0 10 142 448 606
Size: 10 132 306 158
Fragments arranged by size:
306 158 132 10
NotI GC'GGCC_GC
Cuts at: 0 79 606
Size: 79 527
NspI r_CATG'y
Cuts at: 0 414 606
Size: 414 192
PasI CC'CwG_GG
Cuts at: 0 278 606
Size: 278 328
PspOMI G'GGCC_C
Cuts at: 0 579 606
Size: 579 27
PstI C_TGCA'G
Cuts at: 0 176 606
Size: 176 430
PvuII CAG'CTG
Cuts at: 0 123 177 606
Size: 123 54 429
Fragments arranged by size:
429 123 54
RsrII CG'GwC_CG
Cuts at: 0 96 606
Size: 96 510
SacII CC_GC'GG
Cuts at: 0 47 85 606
Size: 47 38 521
Fragments arranged by size:
521 47 38
SfcI C'TryA_G
Cuts at: 0 172 285 606
Size: 172 113 321
Fragments arranged by size:
321 172 113
SfoI GGC'GCC
Cuts at: 0 51 70 133 564 606
Size: 51 19 63 431 42
Fragments arranged by size:
431 63 51 42 19
SgrAI Cr'CCGG_yG
Cuts at: 0 448 606
Size: 448 158
StuI AGG'CCT
Cuts at: 0 475 606
Size: 475 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 606
Size: 573 33
Enzymes that do cut:
AarI AccI Acc65I AcuI ApaI AvaI BanI BanII
BbeI BglI Bme1580I BmgBI BpmI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII
BstXI BtgI EaeI EagI Eco57MI EcoO109I FspI HaeII
KasI KpnI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PasI PspOMI PstI PvuII RsrII SacII SfcI
SfoI SgrAI StuI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI Hin4I HincII HindIII HpaI MfeI
MluI MmeI MscI NcoI NdeI NheI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI