Restriction map for
pFN21AB5020
(Linear) (Six-base) MAPSORT of: KIBB5020.seq Check: 7232 from: 1 to: 1665
KIBB5020.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
Acc65I G'GTAC_C
Cuts at: 0 824 1394 1665
Size: 824 570 271
Fragments arranged by size:
824 570 271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 538 589 1209 1665
Size: 448 90 51 620 456
Fragments arranged by size:
620 456 448 90 51
AflIII A'CryG_T
Cuts at: 0 1103 1665
Size: 1103 562
AgeI A'CCGG_T
Cuts at: 0 1647 1665
Size: 1647 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 1665
Size: 1327 32 306
Fragments arranged by size:
1327 306 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1200 1665
Size: 1200 465
ApoI r'AATT_y
Cuts at: 0 252 1665
Size: 252 1413
AvaI C'yCGr_G
Cuts at: 0 802 1045 1665
Size: 802 243 620
Fragments arranged by size:
802 620 243
BanI G'GyrC_C
Cuts at: 0 142 824 1394 1665
Size: 142 682 570 271
Fragments arranged by size:
682 570 271 142
BanII G_rGCy'C
Cuts at: 0 769 1307 1665
Size: 769 538 358
Fragments arranged by size:
769 538 358
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 1665
Size: 12 1653
BbvCI CC'TCA_GC
Cuts at: 0 1178 1312 1665
Size: 1178 134 353
Fragments arranged by size:
1178 353 134
BciVI GTATCCnnnnn_n'
Cuts at: 0 182 1665
Size: 182 1483
BfrBI ATG'CAT
Cuts at: 0 1257 1665
Size: 1257 408
BglII A'GATC_T
Cuts at: 0 491 1665
Size: 491 1174
Bme1580I G_kGCm'C
Cuts at: 0 1571 1665
Size: 1571 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 524 979 1014 1129 1665
Size: 177 347 455 35 115 536
Fragments arranged by size:
536 455 347 177 115 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 783 1665
Size: 783 882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 658 1665
Size: 301 357 1007
Fragments arranged by size:
1007 357 301
Bpu10I CC'TnA_GC
Cuts at: 0 334 1178 1312 1665
Size: 334 844 134 353
Fragments arranged by size:
844 353 334 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1665
Size: 965 700
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 1659 1665
Size: 220 1439 6
Fragments arranged by size:
1439 220 6
BsaAI yAC'GTr
Cuts at: 0 723 1665
Size: 723 942
BsaHI Gr'CG_yC
Cuts at: 0 62 1665
Size: 62 1603
BsaWI w'CCGG_w
Cuts at: 0 649 1647 1665
Size: 649 998 18
Fragments arranged by size:
998 649 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 893 1345 1665
Size: 94 799 452 320
Fragments arranged by size:
799 452 320 94
BseYI C'CCAG_C
Cuts at: 0 410 762 1306 1511 1665
Size: 410 352 544 205 154
Fragments arranged by size:
544 410 352 205 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1665
Size: 1372 293
BsiEI CG_ry'CG
Cuts at: 0 17 72 387 1665
Size: 17 55 315 1278
Fragments arranged by size:
1278 315 55 17
BsiHKAI G_wGCw'C
Cuts at: 0 223 1496 1665
Size: 223 1273 169
Fragments arranged by size:
1273 223 169
Bsp1286I G_dGCh'C
Cuts at: 0 223 769 1307 1496 1571 1665
Size: 223 546 538 189 75 94
Fragments arranged by size:
546 538 223 189 94 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 841 1665
Size: 841 824
BsrFI r'CCGG_y
Cuts at: 0 1647 1665
Size: 1647 18
BssHII G'CGCG_C
Cuts at: 0 9 1665
Size: 9 1656
BstAPI GCAn_nnn'nTGC
Cuts at: 0 218 1665
Size: 218 1447
BstEII G'GTnAC_C
Cuts at: 0 594 853 951 1665
Size: 594 259 98 714
Fragments arranged by size:
714 594 259 98
BstYI r'GATC_y
Cuts at: 0 491 830 1448 1607 1665
Size: 491 339 618 159 58
Fragments arranged by size:
618 491 339 159 58
BtgI C'CryG_G
Cuts at: 0 714 792 1665
Size: 714 78 873
Fragments arranged by size:
873 714 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1250 1665
Size: 1250 415
BtsI GCAGTG_nn'
Cuts at: 0 992 1665
Size: 992 673
EaeI y'GGCC_r
Cuts at: 0 14 69 996 1064 1665
Size: 14 55 927 68 601
Fragments arranged by size:
927 601 68 55 14
EagI C'GGCC_G
Cuts at: 0 14 69 1665
Size: 14 55 1596
Fragments arranged by size:
1596 55 14
EarI CTCTTCn'nnn_
Cuts at: 0 1042 1228 1295 1665
Size: 1042 186 67 370
Fragments arranged by size:
1042 370 186 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1492 1665
Size: 1492 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 448 538 589 658 1209 1665
Size: 301 147 90 51 69 551 456
Fragments arranged by size:
551 456 301 147 90 69 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 607 1665
Size: 607 1058
EcoO109I rG'GnC_Cy
Cuts at: 0 84 861 1381 1665
Size: 84 777 520 284
Fragments arranged by size:
777 520 284 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 453 1665
Size: 453 1212
HaeII r_GCGC'y
Cuts at: 0 46 94 1665
Size: 46 48 1571
Fragments arranged by size:
1571 48 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 822 854 1433 1465 1665
Size: 822 32 579 32 200
Fragments arranged by size:
822 579 200 32 32
HindIII A'AGCT_T
Cuts at: 0 1434 1665
Size: 1434 231
KpnI G_GTAC'C
Cuts at: 0 828 1398 1665
Size: 828 570 267
Fragments arranged by size:
828 570 267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1665
Size: 1186 479
MscI TGG'CCA
Cuts at: 0 998 1066 1665
Size: 998 68 599
Fragments arranged by size:
998 599 68
MslI CAynn'nnrTG
Cuts at: 0 931 1018 1665
Size: 931 87 647
Fragments arranged by size:
931 647 87
MspA1I CmG'CkG
Cuts at: 0 52 414 1665
Size: 52 362 1251
Fragments arranged by size:
1251 362 52
NcoI C'CATG_G
Cuts at: 0 714 792 1665
Size: 714 78 873
Fragments arranged by size:
873 714 78
NsiI A_TGCA'T
Cuts at: 0 1259 1665
Size: 1259 406
NspI r_CATG'y
Cuts at: 0 1017 1107 1665
Size: 1017 90 558
Fragments arranged by size:
1017 558 90
PasI CC'CwG_GG
Cuts at: 0 737 1665
Size: 737 928
PciI A'CATG_T
Cuts at: 0 1103 1665
Size: 1103 562
PflMI CCAn_nnn'nTGG
Cuts at: 0 374 1518 1665
Size: 374 1144 147
Fragments arranged by size:
1144 374 147
PfoI T'CCnGG_A
Cuts at: 0 608 959 1665
Size: 608 351 706
Fragments arranged by size:
706 608 351
PpuMI rG'GwC_Cy
Cuts at: 0 861 1381 1665
Size: 861 520 284
Fragments arranged by size:
861 520 284
PstI C_TGCA'G
Cuts at: 0 214 1665
Size: 214 1451
PvuII CAG'CTG
Cuts at: 0 414 1665
Size: 414 1251
SapI GCTCTTCn'nnn_
Cuts at: 0 1295 1665
Size: 1295 370
SfcI C'TryA_G
Cuts at: 0 210 1665
Size: 210 1455
SgrAI Cr'CCGG_yG
Cuts at: 0 1647 1665
Size: 1647 18
SmlI C'TyrA_G
Cuts at: 0 980 1665
Size: 980 685
SphI G_CATG'C
Cuts at: 0 1017 1665
Size: 1017 648
SspI AAT'ATT
Cuts at: 0 922 1665
Size: 922 743
StyI C'CwwG_G
Cuts at: 0 714 792 1665
Size: 714 78 873
Fragments arranged by size:
873 714 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 1006 1665
Size: 375 631 659
Fragments arranged by size:
659 631 375
XmnI GAAnn'nnTTC
Cuts at: 0 346 1486 1665
Size: 346 1140 179
Fragments arranged by size:
1140 346 179
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AloI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BciVI BfrBI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssHII BstAPI BstEII BstYI BtgI BtgZI BtsI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I
NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI
PstI PvuII SapI SfcI SgrAI SmlI SphI SspI
StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BclI BglI BlpI BmgBI BmtI
BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssSI BstBI BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SnaBI SpeI SrfI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
ZraI