Restriction map for
pFN21AB4854
(Linear) (Six-base) MAPSORT of: KIBB4854.seq Check: 1083 from: 1 to: 3678
KIBB4854.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 606 2351 2433 3678
Size: 606 1745 82 1245
Fragments arranged by size:
1745 1245 606 82
AatII G_ACGT'C
Cuts at: 0 1511 3678
Size: 1511 2167
AccI GT'mk_AC
Cuts at: 0 3433 3678
Size: 3433 245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 562 772 2143 2711 3035 3678
Size: 141 421 210 1371 568 324 643
Fragments arranged by size:
1371 643 568 421 324 210 141
AflII C'TTAA_G
Cuts at: 0 1108 3678
Size: 1108 2570
AhdI GACnn_n'nnGTC
Cuts at: 0 42 2043 3678
Size: 42 2001 1635
Fragments arranged by size:
2001 1635 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1936 2586 3678
Size: 1936 650 1092
Fragments arranged by size:
1936 1092 650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 620 652 2206 2238 3678
Size: 620 32 1554 32 1440
Fragments arranged by size:
1554 1440 620 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 403 1886 2121 2885 3645 3678
Size: 403 1483 235 764 760 33
Fragments arranged by size:
1483 764 760 403 235 33
ApaI G_GGCC'C
Cuts at: 0 2163 3678
Size: 2163 1515
ApaLI G'TGCA_C
Cuts at: 0 775 3678
Size: 775 2903
ApoI r'AATT_y
Cuts at: 0 982 3559 3678
Size: 982 2577 119
Fragments arranged by size:
2577 982 119
AvaI C'yCGr_G
Cuts at: 0 40 542 1813 3678
Size: 40 502 1271 1865
Fragments arranged by size:
1865 1271 502 40
AvrII C'CTAG_G
Cuts at: 0 2155 3678
Size: 2155 1523
BamHI G'GATC_C
Cuts at: 0 203 2330 3678
Size: 203 2127 1348
Fragments arranged by size:
2127 1348 203
BanI G'GyrC_C
Cuts at: 0 455 1218 1795 3678
Size: 455 763 577 1883
Fragments arranged by size:
1883 763 577 455
BanII G_rGCy'C
Cuts at: 0 167 354 499 1823 1871 2163 3113 3678
Size: 167 187 145 1324 48 292 950 565
Fragments arranged by size:
1324 950 565 292 187 167 145 48
BbeI G_GCGC'C
Cuts at: 0 1799 3678
Size: 1799 1879
BbsI GAAGACnn'nnnn_
Cuts at: 0 752 2483 2898 3173 3678
Size: 752 1731 415 275 505
Fragments arranged by size:
1731 752 505 415 275
BbvCI CC'TCA_GC
Cuts at: 0 1782 3678
Size: 1782 1896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3008 3042 3678
Size: 3008 34 636
Fragments arranged by size:
3008 636 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1026 3678
Size: 1026 2652
BclI T'GATC_A
Cuts at: 0 2112 3678
Size: 2112 1566
BfrBI ATG'CAT
Cuts at: 0 76 2656 3678
Size: 76 2580 1022
Fragments arranged by size:
2580 1022 76
BglI GCCn_nnn'nGGC
Cuts at: 0 607 1612 1764 3678
Size: 607 1005 152 1914
Fragments arranged by size:
1914 1005 607 152
BglII A'GATC_T
Cuts at: 0 880 3678
Size: 880 2798
BlpI GC'TnA_GC
Cuts at: 0 1114 1963 3678
Size: 1114 849 1715
Fragments arranged by size:
1715 1114 849
Bme1580I G_kGCm'C
Cuts at: 0 458 779 1703 2163 3678
Size: 458 321 924 460 1515
Fragments arranged by size:
1515 924 460 458 321
BmgBI CAC'GTC
Cuts at: 0 3483 3678
Size: 3483 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 3531 3678
Size: 3531 147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1178 1308 1338 1797 1941 3678
Size: 1178 130 30 459 144 1737
Fragments arranged by size:
1737 1178 459 144 130 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 905 1257 1308 1344 2062 2233 2846
Size: 10 895 352 51 36 718 171 613
Cuts at: 2846 3678
Size: 832
Fragments arranged by size:
895 832 718 613 352 171 51 36
10
Bpu10I CC'TnA_GC
Cuts at: 0 746 1782 3678
Size: 746 1036 1896
Fragments arranged by size:
1896 1036 746
BsaI GGTCTCn'nnnn_
Cuts at: 0 1303 2297 2777 2864 3398 3678
Size: 1303 994 480 87 534 280
Fragments arranged by size:
1303 994 534 480 280 87
BsaHI Gr'CG_yC
Cuts at: 0 1508 1796 3678
Size: 1508 288 1882
Fragments arranged by size:
1882 1508 288
BsaWI w'CCGG_w
Cuts at: 0 200 1294 3678
Size: 200 1094 2384
Fragments arranged by size:
2384 1094 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 509 539 2206 2236 3678
Size: 509 30 1667 30 1442
Fragments arranged by size:
1667 1442 509 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 1144 1260 1600 1831 1917 2013 2016
Size: 886 258 116 340 231 86 96 3
Cuts at: 2016 2071 2091 2230 3678
Size: 55 20 139 1448
Fragments arranged by size:
1448 886 340 258 231 139 116 96
86 55 20 3
BseYI C'CCAG_C
Cuts at: 0 451 670 1546 1839 2404 3678
Size: 451 219 876 293 565 1274
Fragments arranged by size:
1274 876 565 451 293 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 765 3490 3586 3631 3678
Size: 433 332 2725 96 45 47
Fragments arranged by size:
2725 433 332 96 47 45
BsiEI CG_ry'CG
Cuts at: 0 695 1658 1829 2632 2842 3678
Size: 695 963 171 803 210 836
Fragments arranged by size:
963 836 803 695 210 171
BsiHKAI G_wGCw'C
Cuts at: 0 779 1576 3113 3340 3620 3678
Size: 779 797 1537 227 280 58
Fragments arranged by size:
1537 797 779 280 227 58
Bsp1286I G_dGCh'C
Cuts at: 0 167 354 458 499 779 1576 1703 1823
Size: 167 187 104 41 280 797 127 120
Cuts at: 1823 1871 2163 3113 3340 3620 3678
Size: 48 292 950 227 280 58
Fragments arranged by size:
950 797 292 280 280 227 187 167
127 120 104 58 48 41
BspEI T'CCGG_A
Cuts at: 0 1294 3678
Size: 1294 2384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 606 1945 2351 2433 3678
Size: 606 1339 406 82 1245
Fragments arranged by size:
1339 1245 606 406 82
BsrDI GCAATG_nn'
Cuts at: 0 567 2984 3056 3678
Size: 567 2417 72 622
Fragments arranged by size:
2417 622 567 72
BsrFI r'CCGG_y
Cuts at: 0 1377 1808 3591 3678
Size: 1377 431 1783 87
Fragments arranged by size:
1783 1377 431 87
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3654 3678
Size: 3654 24
BstEII G'GTnAC_C
Cuts at: 0 3594 3678
Size: 3594 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1519 2211 3317 3387 3678
Size: 1519 692 1106 70 291
Fragments arranged by size:
1519 1106 692 291 70
BstYI r'GATC_y
Cuts at: 0 203 256 880 1297 2330 3576 3678
Size: 203 53 624 417 1033 1246 102
Fragments arranged by size:
1246 1033 624 417 203 102 53
Bsu36I CC'TnA_GG
Cuts at: 0 2750 3678
Size: 2750 928
BtgI C'CryG_G
Cuts at: 0 602 1187 1464 1652 1658 1664 1712 1745
Size: 602 585 277 188 6 6 48 33
Cuts at: 1745 1823 1829 2084 2854 3065 3316 3386 3678
Size: 78 6 255 770 211 251 70 292
Fragments arranged by size:
770 602 585 292 277 255 251 211
188 78 70 48 33 6 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2184 3678
Size: 2184 1494
BtsI GCAGTG_nn'
Cuts at: 0 777 1125 1703 2154 2568 2931 3678
Size: 777 348 578 451 414 363 747
Fragments arranged by size:
777 747 578 451 414 363 348
ClaI AT'CG_AT
Cuts at: 0 323 3678
Size: 323 3355
DraIII CAC_nnn'GTG
Cuts at: 0 52 3678
Size: 52 3626
DrdI GACnn_nn'nnGTC
Cuts at: 0 2007 2322 3678
Size: 2007 315 1356
Fragments arranged by size:
2007 1356 315
EaeI y'GGCC_r
Cuts at: 0 471 608 717 1655 1661 1826 2244 2534
Size: 471 137 109 938 6 165 418 290
Cuts at: 2534 2839 2874 3473 3530 3542 3678
Size: 305 35 599 57 12 136
Fragments arranged by size:
938 599 471 418 305 290 165 137
136 109 57 35 12 6
EagI C'GGCC_G
Cuts at: 0 1655 1826 2839 3678
Size: 1655 171 1013 839
Fragments arranged by size:
1655 1013 839 171
EarI CTCTTCn'nnn_
Cuts at: 0 160 1121 2529 2739 3678
Size: 160 961 1408 210 939
Fragments arranged by size:
1408 961 939 210 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2795 3234 3678
Size: 2795 439 444
Fragments arranged by size:
2795 444 439
EcoICRI GAG'CTC
Cuts at: 0 3111 3678
Size: 3111 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 141 562 772 905 1257 1308 1344
Size: 10 131 421 210 133 352 51 36
Cuts at: 1344 2062 2143 2233 2711 2846 3035 3678
Size: 718 81 90 478 135 189 643
Fragments arranged by size:
718 643 478 421 352 210 189 135
133 131 90 81 51 36 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 2347 3544 3678
Size: 25 2322 1197 134
Fragments arranged by size:
2322 1197 134 25
EcoO109I rG'GnC_Cy
Cuts at: 0 36 506 729 1614 1778 1883 2159 2160
Size: 36 470 223 885 164 105 276 1
Cuts at: 2160 3061 3148 3642 3678
Size: 901 87 494 36
Fragments arranged by size:
901 885 494 470 276 223 164 105
87 36 36 1
EcoRI G'AATT_C
Cuts at: 0 3559 3678
Size: 3559 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1123 3678
Size: 1123 2555
HaeII r_GCGC'y
Cuts at: 0 1187 1799 1950 2372 3422 3668 3678
Size: 1187 612 151 422 1050 246 10
Fragments arranged by size:
1187 1050 612 422 246 151 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 178 210 865 897 1990 1996
Size: 28 32 118 32 655 32 1093 6
Cuts at: 1996 2022 2028 2029 2061 2275 2277 2307 2309
Size: 26 6 1 32 214 2 30 2
Cuts at: 2309 3678
Size: 1369
Fragments arranged by size:
1369 1093 655 214 118 32 32 32
32 30 28 26 6 6 2 2
1
HincII GTy'rAC
Cuts at: 0 3434 3678
Size: 3434 244
KasI G'GCGC_C
Cuts at: 0 1795 3678
Size: 1795 1883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 258 2027 2060 2894 3434 3678
Size: 221 37 1769 33 834 540 244
Fragments arranged by size:
1769 834 540 244 221 37 33
MscI TGG'CCA
Cuts at: 0 2536 3544 3678
Size: 2536 1008 134
Fragments arranged by size:
2536 1008 134
MslI CAynn'nnrTG
Cuts at: 0 3315 3678
Size: 3315 363
MspA1I CmG'CkG
Cuts at: 0 403 626 899 1256 1401 1654 1714 1747
Size: 403 223 273 357 145 253 60 33
Cuts at: 1747 1825 1831 2134 2766 3678
Size: 78 6 303 632 912
Fragments arranged by size:
912 632 403 357 303 273 253 223
145 78 60 33 6
NarI GG'CG_CC
Cuts at: 0 1796 3678
Size: 1796 1882
NcoI C'CATG_G
Cuts at: 0 602 3065 3678
Size: 602 2463 613
Fragments arranged by size:
2463 613 602
NotI GC'GGCC_GC
Cuts at: 0 1826 3678
Size: 1826 1852
NsiI A_TGCA'T
Cuts at: 0 78 2658 3678
Size: 78 2580 1020
Fragments arranged by size:
2580 1020 78
PasI CC'CwG_GG
Cuts at: 0 1618 2505 3678
Size: 1618 887 1173
Fragments arranged by size:
1618 1173 887
PflMI CCAn_nnn'nTGG
Cuts at: 0 1072 3468 3678
Size: 1072 2396 210
Fragments arranged by size:
2396 1072 210
PfoI T'CCnGG_A
Cuts at: 0 26 917 1511 2104 2326 3678
Size: 26 891 594 593 222 1352
Fragments arranged by size:
1352 891 594 593 222 26
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 2206 2238 3678
Size: 349 32 1825 32 1440
Fragments arranged by size:
1825 1440 349 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 36 506 1778 3678
Size: 36 470 1272 1900
Fragments arranged by size:
1900 1272 470 36
PshAI GACnn'nnGTC
Cuts at: 0 3439 3678
Size: 3439 239
PspOMI G'GGCC_C
Cuts at: 0 2159 3678
Size: 2159 1519
PspXI vC'TCGA_Gb
Cuts at: 0 40 3678
Size: 40 3638
PstI C_TGCA'G
Cuts at: 0 1210 2430 2771 3678
Size: 1210 1220 341 907
Fragments arranged by size:
1220 1210 907 341
PvuII CAG'CTG
Cuts at: 0 403 899 1401 2766 3678
Size: 403 496 502 1365 912
Fragments arranged by size:
1365 912 502 496 403
SacI G_AGCT'C
Cuts at: 0 3113 3678
Size: 3113 565
SacII CC_GC'GG
Cuts at: 0 1655 1715 1748 1826 1832 3678
Size: 1655 60 33 78 6 1846
Fragments arranged by size:
1846 1655 78 60 33 6
SalI G'TCGA_C
Cuts at: 0 3432 3678
Size: 3432 246
SapI GCTCTTCn'nnn_
Cuts at: 0 2529 3678
Size: 2529 1149
ScaI AGT'ACT
Cuts at: 0 1486 3631 3678
Size: 1486 2145 47
Fragments arranged by size:
2145 1486 47
SexAI A'CCwGG_T
Cuts at: 0 3620 3678
Size: 3620 58
SfcI C'TryA_G
Cuts at: 0 375 1206 2165 2426 2484 2767 3678
Size: 375 831 959 261 58 283 911
Fragments arranged by size:
959 911 831 375 283 261 58
SfoI GGC'GCC
Cuts at: 0 1797 3678
Size: 1797 1881
SmlI C'TyrA_G
Cuts at: 0 40 1108 3678
Size: 40 1068 2570
Fragments arranged by size:
2570 1068 40
StyI C'CwwG_G
Cuts at: 0 602 830 1597 1985 2155 2204 2381 3065
Size: 602 228 767 388 170 49 177 684
Cuts at: 3065 3545 3678
Size: 480 133
Fragments arranged by size:
767 684 602 480 388 228 177 170
133 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 473 1327 2618 3678
Size: 473 854 1291 1060
Fragments arranged by size:
1291 1060 854 473
TatI w'GTAC_w
Cuts at: 0 1484 3212 3629 3678
Size: 1484 1728 417 49
Fragments arranged by size:
1728 1484 417 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2178 2210 3443 3475 3678
Size: 2178 32 1233 32 203
Fragments arranged by size:
2178 1233 203 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2004 3678
Size: 2004 1674
XhoI C'TCGA_G
Cuts at: 0 40 3678
Size: 40 3638
XmnI GAAnn'nnTTC
Cuts at: 0 2819 3678
Size: 2819 859
ZraI GAC'GTC
Cuts at: 0 1509 3678
Size: 1509 2169
Enzymes that do cut:
AarI AatII AccI AcuI AflII AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NarI NcoI NotI NsiI
PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SalI SapI ScaI SexAI
SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I
XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AgeI AleI AscI AseI
AsiSI BaeI BmtI BpuEI BsaAI BsaBI BsiWI BsmI
BsmBI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I
CspCI DraI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NruI
NspI PacI PciI PmeI PmlI PsiI PsrI PvuI
RsrII SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XcmI
XmaI