(Linear) (Six-base) MAPSORT of: KIBB4262.seq  Check: 3497  from: 1  to: 531



KIBB4262.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     377     531 
   Size:        377     154
 
AccI GT'mk_AC

Cuts at:      0      33     531 
   Size:         33     498
 
AlwNI CAG_nnn'CTG

Cuts at:      0     400     531 
   Size:        400     131
 
ApoI r'AATT_y

Cuts at:      0     334     531 
   Size:        334     197
 
BanI G'GyrC_C

Cuts at:      0     259     531 
   Size:        259     272
 
BbvCI CC'TCA_GC

Cuts at:      0     469     531 
   Size:        469      62
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      69     531 
   Size:         69     462
 
Bpu10I CC'TnA_GC

Cuts at:      0     469     531 
   Size:        469      62
 
BsaAI yAC'GTr

Cuts at:      0     330     531 
   Size:        330     201
 
BseYI C'CCAG_C

Cuts at:      0     114     531 
   Size:        114     417
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     531 
   Size:        413     118
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     377     531 
   Size:        377     154
 
BtgI C'CryG_G

Cuts at:      0     451     531 
   Size:        451      80
 
DraI TTT'AAA

Cuts at:      0      27     531 
   Size:         27     504
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     143     531 
   Size:        143     388
 
EcoO109I rG'GnC_Cy

Cuts at:      0     465     531 
   Size:        465      66
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     310     383     531 
   Size:        310      73     148

  Fragments arranged by size: 

                 310     148      73
 
MspA1I CmG'CkG

Cuts at:      0      82     302     411     476     520     531 
   Size:         82     220     109      65      44      11

  Fragments arranged by size: 

                 220     109      82      65      44      11
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     509     531 
   Size:        509      22
 
PmlI CAC'GTG

Cuts at:      0     330     531 
   Size:        330     201
 
PpuMI rG'GwC_Cy

Cuts at:      0     465     531 
   Size:        465      66
 
PvuII CAG'CTG

Cuts at:      0      82     411     520     531 
   Size:         82     329     109      11

  Fragments arranged by size: 

                 329     109      82      11
 
SfcI C'TryA_G

Cuts at:      0     140     531 
   Size:        140     391
 

 Enzymes that do cut:

     AarI     AccI    AlwNI     ApoI     BanI    BbvCI    BciVI   Bpu10I
    BsaAI    BseYI     BsgI    BspMI     BtgI     DraI    EcoNI EcoO109I
     FalI   MspA1I    PflMI     PmlI    PpuMI    PvuII     SfcI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI