Restriction map for
pFN21AB4243
(Linear) (Six-base) MAPSORT of: KIBB4243.seq Check: 6415 from: 1 to: 4515
KIBB4243.orf
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 666 775 910 4515
Size: 666 109 135 3605
Fragments arranged by size:
3605 666 135 109
AccI GT'mk_AC
Cuts at: 0 1358 4515
Size: 1358 3157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1744 2452 4515
Size: 1744 708 2063
Fragments arranged by size:
2063 1744 708
AfeI AGC'GCT
Cuts at: 0 229 4063 4515
Size: 229 3834 452
Fragments arranged by size:
3834 452 229
AflIII A'CryG_T
Cuts at: 0 308 1126 3047 3696 3749 4515
Size: 308 818 1921 649 53 766
Fragments arranged by size:
1921 818 766 649 308 53
AleI CACnn'nnGTG
Cuts at: 0 769 904 1792 2963 3437 3634 4310 4515
Size: 769 135 888 1171 474 197 676 205
Fragments arranged by size:
1171 888 769 676 474 205 197 135
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3703 3735 4515
Size: 3703 32 780
Fragments arranged by size:
3703 780 32
AlwNI CAG_nnn'CTG
Cuts at: 0 285 1048 1776 3672 4515
Size: 285 763 728 1896 843
Fragments arranged by size:
1896 843 763 728 285
ApaI G_GGCC'C
Cuts at: 0 19 421 1208 1505 4515
Size: 19 402 787 297 3010
Fragments arranged by size:
3010 787 402 297 19
AvaI C'yCGr_G
Cuts at: 0 832 976 1032 3715 4154 4515
Size: 832 144 56 2683 439 361
Fragments arranged by size:
2683 832 439 361 144 56
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4468 4501 4515
Size: 4468 33 14
Fragments arranged by size:
4468 33 14
BamHI G'GATC_C
Cuts at: 0 275 525 4515
Size: 275 250 3990
Fragments arranged by size:
3990 275 250
BanI G'GyrC_C
Cuts at: 0 3 1780 2193 2328 4515
Size: 3 1777 413 135 2187
Fragments arranged by size:
2187 1777 413 135 3
BanII G_rGCy'C
Cuts at: 0 19 95 421 573 784 1153 1208 1331
Size: 19 76 326 152 211 369 55 123
Cuts at: 1331 1505 1612 1646 1937 2161 2270 2432 3868
Size: 174 107 34 291 224 109 162 1436
Cuts at: 3868 4515
Size: 647
Fragments arranged by size:
1436 647 369 326 291 224 211 174
162 152 123 109 107 76 55 34
19
BbeI G_GCGC'C
Cuts at: 0 7 1784 4515
Size: 7 1777 2731
Fragments arranged by size:
2731 1777 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1864 2592 3514 3962 4329 4515
Size: 1864 728 922 448 367 186
Fragments arranged by size:
1864 922 728 448 367 186
BbvCI CC'TCA_GC
Cuts at: 0 271 286 4515
Size: 271 15 4229
Fragments arranged by size:
4229 271 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3696 3730 4048 4082 4515
Size: 3696 34 318 34 433
Fragments arranged by size:
3696 433 318 34 34
BclI T'GATC_A
Cuts at: 0 1850 3174 4515
Size: 1850 1324 1341
Fragments arranged by size:
1850 1341 1324
BglI GCCn_nnn'nGGC
Cuts at: 0 2039 3458 4289 4515
Size: 2039 1419 831 226
Fragments arranged by size:
2039 1419 831 226
BglII A'GATC_T
Cuts at: 0 1169 4515
Size: 1169 3346
BlpI GC'TnA_GC
Cuts at: 0 1285 1873 2773 4348 4515
Size: 1285 588 900 1575 167
Fragments arranged by size:
1575 1285 900 588 167
Bme1580I G_kGCm'C
Cuts at: 0 19 421 1208 1505 2333 3353 3641 4515
Size: 19 402 787 297 828 1020 288 874
Fragments arranged by size:
1020 874 828 787 402 297 288 19
BmgBI CAC'GTC
Cuts at: 0 3699 3752 4515
Size: 3699 53 763
Fragments arranged by size:
3699 763 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1468 1626 2029 2344 2446 2747 2959 3169
Size: 1468 158 403 315 102 301 212 210
Cuts at: 3169 3276 3628 4381 4501 4515
Size: 107 352 753 120 14
Fragments arranged by size:
1468 753 403 352 315 301 212 210
158 120 107 102 14
Bpu10I CC'TnA_GC
Cuts at: 0 271 286 1147 4515
Size: 271 15 861 3368
Fragments arranged by size:
3368 861 271 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1969 2809 2827 3508 4515
Size: 1969 840 18 681 1007
Fragments arranged by size:
1969 1007 840 681 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 1450 1579 3073 3196 3940 4515
Size: 1450 129 1494 123 744 575
Fragments arranged by size:
1494 1450 744 575 129 123
BsaAI yAC'GTr
Cuts at: 0 672 798 1025 1710 3024 3531 4515
Size: 672 126 227 685 1314 507 984
Fragments arranged by size:
1314 984 685 672 507 227 126
BsaHI Gr'CG_yC
Cuts at: 0 4 926 1781 3039 3479 4334 4515
Size: 4 922 855 1258 440 855 181
Fragments arranged by size:
1258 922 855 855 440 181 4
BsaWI w'CCGG_w
Cuts at: 0 1374 4515
Size: 1374 3141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2848 2878 3103 3133 3142 3172 3565 3595
Size: 2848 30 225 30 9 30 393 30
Cuts at: 3595 4515
Size: 920
Fragments arranged by size:
2848 920 393 225 30 30 30 30
9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 642 1330 1636 2572 3166 4515
Size: 642 688 306 936 594 1349
Fragments arranged by size:
1349 936 688 642 594 306
BseYI C'CCAG_C
Cuts at: 0 1210 1877 2095 2235 2537 3548 4421 4515
Size: 1210 667 218 140 302 1011 873 94
Fragments arranged by size:
1210 1011 873 667 302 218 140 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 278 488 1327 3262 3586 4459 4515
Size: 101 177 210 839 1935 324 873 56
Fragments arranged by size:
1935 873 839 324 210 177 101 56
BsiEI CG_ry'CG
Cuts at: 0 1184 3121 3995 4058 4429 4515
Size: 1184 1937 874 63 371 86
Fragments arranged by size:
1937 1184 874 371 86 63
BsiHKAI G_wGCw'C
Cuts at: 0 1382 1478 1646 2270 2813 3829 4515
Size: 1382 96 168 624 543 1016 686
Fragments arranged by size:
1382 1016 686 624 543 168 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 229 2736 4515
Size: 229 2507 1779
Fragments arranged by size:
2507 1779 229
Bsp1286I G_dGCh'C
Cuts at: 0 19 95 421 573 784 1153 1208 1331
Size: 19 76 326 152 211 369 55 123
Cuts at: 1331 1382 1478 1505 1612 1646 1937 2161 2270
Size: 51 96 27 107 34 291 224 109
Cuts at: 2270 2333 2432 2813 3353 3641 3829 3868 4515
Size: 63 99 381 540 288 188 39 647
Fragments arranged by size:
647 540 381 369 326 291 288 224
211 188 152 123 109 107 99 96
76 63 55 51 39 34 27 19
BspHI T'CATG_A
Cuts at: 0 1853 3134 3539 4515
Size: 1853 1281 405 976
Fragments arranged by size:
1853 1281 976 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 666 775 910 1571 2087 3406 4515
Size: 666 109 135 661 516 1319 1109
Fragments arranged by size:
1319 1109 666 661 516 135 109
BsrBI CCG'CTC
Cuts at: 0 441 1763 2820 3492 4365 4515
Size: 441 1322 1057 672 873 150
Fragments arranged by size:
1322 1057 873 672 441 150
BsrDI GCAATG_nn'
Cuts at: 0 546 2618 3053 4515
Size: 546 2072 435 1462
Fragments arranged by size:
2072 1462 546 435
BsrFI r'CCGG_y
Cuts at: 0 637 1320 3035 3415 3442 4053 4315 4428
Size: 637 683 1715 380 27 611 262 113
Cuts at: 4428 4515
Size: 87
Fragments arranged by size:
1715 683 637 611 380 262 113 87
27
BssHII G'CGCG_C
Cuts at: 0 1256 4515
Size: 1256 3259
BssSI C'ACGA_G
Cuts at: 0 185 733 2210 3595 4515
Size: 185 548 1477 1385 920
Fragments arranged by size:
1477 1385 920 548 185
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1542 2684 3461 4515
Size: 1542 1142 777 1054
Fragments arranged by size:
1542 1142 1054 777
BstEII G'GTnAC_C
Cuts at: 0 172 1224 2055 4515
Size: 172 1052 831 2460
Fragments arranged by size:
2460 1052 831 172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1930 3024 3246 4515
Size: 1930 1094 222 1269
Fragments arranged by size:
1930 1269 1094 222
BstYI r'GATC_y
Cuts at: 0 275 525 1044 1169 1919 2256 4092 4515
Size: 275 250 519 125 750 337 1836 423
Fragments arranged by size:
1836 750 519 423 337 275 250 125
BtgI C'CryG_G
Cuts at: 0 44 167 295 776 848 986 1220 1261
Size: 44 123 128 481 72 138 234 41
Cuts at: 1261 1344 1367 1385 1433 1615 1665 1793 2594
Size: 83 23 18 48 182 50 128 801
Cuts at: 2594 3342 3614 3950 4433 4515
Size: 748 272 336 483 82
Fragments arranged by size:
801 748 483 481 336 272 234 182
138 128 128 123 83 82 72 50
48 44 41 23 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2778 2931 3906 4515
Size: 2778 153 975 609
Fragments arranged by size:
2778 975 609 153
BtsI GCAGTG_nn'
Cuts at: 0 272 594 1315 2197 3006 3432 3444 4305
Size: 272 322 721 882 809 426 12 861
Cuts at: 4305 4317 4515
Size: 12 198
Fragments arranged by size:
882 861 809 721 426 322 272 198
12 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1284 1319 2193 2228 4515
Size: 1284 35 874 35 2287
Fragments arranged by size:
2287 1284 874 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1386 1410 4515
Size: 1386 24 3105
Fragments arranged by size:
3105 1386 24
EaeI y'GGCC_r
Cuts at: 0 623 773 890 908 1181 1197 1250 1668
Size: 623 150 117 18 273 16 53 418
Cuts at: 1668 1953 2151 2433 2591 3248 3334 3525 4051
Size: 285 198 282 158 657 86 191 526
Cuts at: 4051 4055 4161 4201 4251 4281 4426 4430 4515
Size: 4 106 40 50 30 145 4 85
Fragments arranged by size:
657 623 526 418 285 282 273 198
191 158 150 145 117 106 86 85
53 50 40 30 18 16 4 4
EagI C'GGCC_G
Cuts at: 0 1181 4055 4426 4515
Size: 1181 2874 371 89
Fragments arranged by size:
2874 1181 371 89
EarI CTCTTCn'nnn_
Cuts at: 0 83 1654 2258 2455 2467 4515
Size: 83 1571 604 197 12 2048
Fragments arranged by size:
2048 1571 604 197 83 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 682 1738 2821 3301 3958 4515
Size: 682 1056 1083 480 657 557
Fragments arranged by size:
1083 1056 682 657 557 480
EcoICRI GAG'CTC
Cuts at: 0 1644 2268 4515
Size: 1644 624 2247
Fragments arranged by size:
2247 1644 624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1468 1626 1744 2029 2344 2446 2452 2747
Size: 1468 158 118 285 315 102 6 295
Cuts at: 2747 2959 3169 3276 3628 4381 4501 4515
Size: 212 210 107 352 753 120 14
Fragments arranged by size:
1468 753 352 315 295 285 212 210
158 120 118 107 102 14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 1447 3607 4515
Size: 376 1071 2160 908
Fragments arranged by size:
2160 1071 908 376
EcoO109I rG'GnC_Cy
Cuts at: 0 15 16 822 1204 1205 1245 1502 1685
Size: 15 1 806 382 1 40 257 183
Cuts at: 1685 1719 1773 2421 2727 3545 3600 4515
Size: 34 54 648 306 818 55 915
Fragments arranged by size:
915 818 806 648 382 306 257 183
55 54 40 34 15 1 1
FseI GG_CCGG'CC
Cuts at: 0 4057 4432 4515
Size: 4057 375 83
Fragments arranged by size:
4057 375 83
FspI TGC'GCA
Cuts at: 0 2203 4515
Size: 2203 2312
HaeII r_GCGC'y
Cuts at: 0 7 231 638 1784 4065 4515
Size: 7 224 407 1146 2281 450
Fragments arranged by size:
2281 1146 450 407 224 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1564 1596 2848 2880 3103 3135
Size: 1036 32 496 32 1252 32 223 32
Cuts at: 3135 3142 3174 3258 3290 4150 4182 4515
Size: 7 32 84 32 860 32 333
Fragments arranged by size:
1252 1036 860 496 333 223 84 32
32 32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 0 219 1188 4515
Size: 219 969 3327
Fragments arranged by size:
3327 969 219
HindIII A'AGCT_T
Cuts at: 0 361 4494 4515
Size: 361 4133 21
Fragments arranged by size:
4133 361 21
KasI G'GCGC_C
Cuts at: 0 3 1780 4515
Size: 3 1777 2735
Fragments arranged by size:
2735 1777 3
MluI A'CGCG_T
Cuts at: 0 3047 4515
Size: 3047 1468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 835 1562 2927 4515
Size: 51 784 727 1365 1588
Fragments arranged by size:
1588 1365 784 727 51
MscI TGG'CCA
Cuts at: 0 625 910 3250 3527 4163 4253 4283 4515
Size: 625 285 2340 277 636 90 30 232
Fragments arranged by size:
2340 636 625 285 277 232 90 30
MslI CAynn'nnrTG
Cuts at: 0 412 769 847 904 1303 1792 2134 2188
Size: 412 357 78 57 399 489 342 54
Cuts at: 2188 2441 2963 3133 3284 3437 3634 3706 4310
Size: 253 522 170 151 153 197 72 604
Cuts at: 4310 4515
Size: 205
Fragments arranged by size:
604 522 489 412 399 357 342 253
205 197 170 153 151 78 72 57
54
MspA1I CmG'CkG
Cuts at: 0 285 297 387 476 558 1263 1346 1617
Size: 285 12 90 89 82 705 83 271
Cuts at: 1617 1877 2235 2322 2520 3072 3117 3939 4425
Size: 260 358 87 198 552 45 822 486
Cuts at: 4425 4515
Size: 90
Fragments arranged by size:
822 705 552 486 358 285 271 260
198 90 90 89 87 83 82 45
12
NaeI GCC'GGC
Cuts at: 0 639 3417 4055 4317 4430 4515
Size: 639 2778 638 262 113 85
Fragments arranged by size:
2778 639 638 262 113 85
NarI GG'CG_CC
Cuts at: 0 4 1781 4515
Size: 4 1777 2734
Fragments arranged by size:
2734 1777 4
NcoI C'CATG_G
Cuts at: 0 44 986 1367 1665 3342 4433 4515
Size: 44 942 381 298 1677 1091 82
Fragments arranged by size:
1677 1091 942 381 298 82 44
NgoMIV G'CCGG_C
Cuts at: 0 637 3415 4053 4315 4428 4515
Size: 637 2778 638 262 113 87
Fragments arranged by size:
2778 638 637 262 113 87
NruI TCG'CGA
Cuts at: 0 3925 4515
Size: 3925 590
NspI r_CATG'y
Cuts at: 0 846 2772 3972 4116 4515
Size: 846 1926 1200 144 399
Fragments arranged by size:
1926 1200 846 399 144
PflMI CCAn_nnn'nTGG
Cuts at: 0 1025 3672 4515
Size: 1025 2647 843
Fragments arranged by size:
2647 1025 843
PfoI T'CCnGG_A
Cuts at: 0 1603 1652 2273 4168 4515
Size: 1603 49 621 1895 347
Fragments arranged by size:
1895 1603 621 347 49
PmlI CAC'GTG
Cuts at: 0 1025 3531 4515
Size: 1025 2506 984
Fragments arranged by size:
2506 1025 984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4444 4476 4515
Size: 4444 32 39
Fragments arranged by size:
4444 39 32
PpuMI rG'GwC_Cy
Cuts at: 0 822 1685 1719 2727 3545 4515
Size: 822 863 34 1008 818 970
Fragments arranged by size:
1008 970 863 822 818 34
PshAI GACnn'nnGTC
Cuts at: 0 3130 4333 4515
Size: 3130 1203 182
Fragments arranged by size:
3130 1203 182
PspOMI G'GGCC_C
Cuts at: 0 15 417 1204 1501 4515
Size: 15 402 787 297 3014
Fragments arranged by size:
3014 787 402 297 15
PspXI vC'TCGA_Gb
Cuts at: 0 3715 4515
Size: 3715 800
PstI C_TGCA'G
Cuts at: 0 578 1817 3439 4312 4515
Size: 578 1239 1622 873 203
Fragments arranged by size:
1622 1239 873 578 203
PvuI CG_AT'CG
Cuts at: 0 3995 4515
Size: 3995 520
PvuII CAG'CTG
Cuts at: 0 387 558 1877 2322 2520 4515
Size: 387 171 1319 445 198 1995
Fragments arranged by size:
1995 1319 445 387 198 171
SacI G_AGCT'C
Cuts at: 0 1646 2270 4515
Size: 1646 624 2245
Fragments arranged by size:
2245 1646 624
SacII CC_GC'GG
Cuts at: 0 298 1264 1347 1618 4515
Size: 298 966 83 271 2897
Fragments arranged by size:
2897 966 298 271 83
SapI GCTCTTCn'nnn_
Cuts at: 0 83 2258 2467 4515
Size: 83 2175 209 2048
Fragments arranged by size:
2175 2048 209 83
ScaI AGT'ACT
Cuts at: 0 4048 4081 4515
Size: 4048 33 434
Fragments arranged by size:
4048 434 33
SexAI A'CCwGG_T
Cuts at: 0 499 4515
Size: 499 4016
SfcI C'TryA_G
Cuts at: 0 574 1813 2290 3435 3900 4308 4515
Size: 574 1239 477 1145 465 408 207
Fragments arranged by size:
1239 1145 574 477 465 408 207
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4289 4515
Size: 4289 226
SfoI GGC'GCC
Cuts at: 0 5 1782 4515
Size: 5 1777 2733
Fragments arranged by size:
2733 1777 5
SgrAI Cr'CCGG_yG
Cuts at: 0 637 4515
Size: 637 3878
SmaI CCC'GGG
Cuts at: 0 978 4156 4515
Size: 978 3178 359
Fragments arranged by size:
3178 978 359
SmlI C'TyrA_G
Cuts at: 0 1984 2824 2842 3487 3715 4515
Size: 1984 840 18 645 228 800
Fragments arranged by size:
1984 840 800 645 228 18
SphI G_CATG'C
Cuts at: 0 2772 3972 4515
Size: 2772 1200 543
Fragments arranged by size:
2772 1200 543
StuI AGG'CCT
Cuts at: 0 3916 4515
Size: 3916 599
StyI C'CwwG_G
Cuts at: 0 44 116 986 1367 1665 2723 3342 3740
Size: 44 72 870 381 298 1058 619 398
Cuts at: 3740 4433 4515
Size: 693 82
Fragments arranged by size:
1058 870 693 619 398 381 298 82
72 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1164 1920 2385 2562 4515
Size: 1164 756 465 177 1953
Fragments arranged by size:
1953 1164 756 465 177
TatI w'GTAC_w
Cuts at: 0 1925 3581 4046 4079 4515
Size: 1925 1656 465 33 436
Fragments arranged by size:
1925 1656 465 436 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2360 2364 2392 2396 3156 3188 4515
Size: 2360 4 28 4 760 32 1327
Fragments arranged by size:
2360 1327 760 32 28 4 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 191 334 391 411 800 918 1218 2398
Size: 191 143 57 20 389 118 300 1180
Cuts at: 2398 2730 2799 3685 3855 4515
Size: 332 69 886 170 660
Fragments arranged by size:
1180 886 660 389 332 300 191 170
143 118 69 57 20
XhoI C'TCGA_G
Cuts at: 0 3715 4515
Size: 3715 800
XmaI C'CCGG_G
Cuts at: 0 976 4154 4515
Size: 976 3178 361
Fragments arranged by size:
3178 976 361
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AloI AlwNI
ApaI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
FspI HaeII Hin4I HincII HindIII KasI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII SacI SacII SapI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BciVI BfrBI BmrI
BmtI BplI BsaBI BsiWI BsmI BspEI BsrGI BstBI
BstZ17I Bsu36I ClaI DraI DrdI EcoRI EcoRV FalI
FspAI HpaI KpnI MfeI NdeI NheI NotI NsiI
PacI PasI PciI PmeI PsiI PsrI RsrII SalI
SanDI SbfI SgfI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XmnI ZraI