Restriction map for
pFN21AB4222
(Linear) (Six-base) MAPSORT of: KIBB4222.seq Check: 3863 from: 1 to: 3021
KIBB4222.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AatII G_ACGT'C
Cuts at: 0 1804 3021
Size: 1804 1217
AccI GT'mk_AC
Cuts at: 0 449 1743 1751 1804 3021
Size: 449 1294 8 53 1217
Fragments arranged by size:
1294 1217 449 53 8
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 2356 2419 3021
Size: 574 1782 63 602
Fragments arranged by size:
1782 602 574 63
AfeI AGC'GCT
Cuts at: 0 361 3021
Size: 361 2660
AflIII A'CryG_T
Cuts at: 0 1329 1612 2637 3021
Size: 1329 283 1025 384
Fragments arranged by size:
1329 1025 384 283
ApaLI G'TGCA_C
Cuts at: 0 2652 3021
Size: 2652 369
ApoI r'AATT_y
Cuts at: 0 499 1156 1460 3021
Size: 499 657 304 1561
Fragments arranged by size:
1561 657 499 304
AvaI C'yCGr_G
Cuts at: 0 2582 3021
Size: 2582 439
BamHI G'GATC_C
Cuts at: 0 307 939 2360 3021
Size: 307 632 1421 661
Fragments arranged by size:
1421 661 632 307
BanI G'GyrC_C
Cuts at: 0 2470 2904 3010 3021
Size: 2470 434 106 11
Fragments arranged by size:
2470 434 106 11
BanII G_rGCy'C
Cuts at: 0 2984 3021
Size: 2984 37
BbeI G_GCGC'C
Cuts at: 0 2474 3021
Size: 2474 547
BciVI GTATCCnnnnn_n'
Cuts at: 0 2518 3021
Size: 2518 503
BclI T'GATC_A
Cuts at: 0 2132 3021
Size: 2132 889
BfrBI ATG'CAT
Cuts at: 0 336 3021
Size: 336 2685
BglII A'GATC_T
Cuts at: 0 167 3021
Size: 167 2854
Bme1580I G_kGCm'C
Cuts at: 0 2656 2907 3021
Size: 2656 251 114
Fragments arranged by size:
2656 251 114
BmgBI CAC'GTC
Cuts at: 0 299 2558 2638 3021
Size: 299 2259 80 383
Fragments arranged by size:
2259 383 299 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1045 1166 1565 1987 2188 2386 2572
Size: 298 747 121 399 422 201 198 186
Cuts at: 2572 2641 2767 3021
Size: 69 126 254
Fragments arranged by size:
747 422 399 298 254 201 198 186
126 121 69
Bpu10I CC'TnA_GC
Cuts at: 0 2873 3021
Size: 2873 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 47 1105 1371 3021
Size: 47 1058 266 1650
Fragments arranged by size:
1650 1058 266 47
BsaAI yAC'GTr
Cuts at: 0 1330 1467 3021
Size: 1330 137 1554
Fragments arranged by size:
1554 1330 137
BsaBI GATnn'nnATC
Cuts at: 0 1930 3021
Size: 1930 1091
BsaHI Gr'CG_yC
Cuts at: 0 329 1801 2471 3021
Size: 329 1472 670 550
Fragments arranged by size:
1472 670 550 329
BsaWI w'CCGG_w
Cuts at: 0 1473 3021
Size: 1473 1548
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 375 405 1520 1550 1563 1593 2545 2575
Size: 375 30 1115 30 13 30 952 30
Cuts at: 2575 3021
Size: 446
Fragments arranged by size:
1115 952 446 375 30 30 30 30
13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 3021
Size: 178 2843
BseYI C'CCAG_C
Cuts at: 0 370 3021
Size: 370 2651
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 3021
Size: 279 2742
BsiHKAI G_wGCw'C
Cuts at: 0 2627 2656 2879 3021
Size: 2627 29 223 142
Fragments arranged by size:
2627 223 142 29
BsmI GAATG_Cn'
Cuts at: 0 69 2183 3021
Size: 69 2114 838
Fragments arranged by size:
2114 838 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2548 3021
Size: 2548 473
Bsp1286I G_dGCh'C
Cuts at: 0 2627 2656 2879 2907 2984 3021
Size: 2627 29 223 28 77 37
Fragments arranged by size:
2627 223 77 37 29 28
BsrDI GCAATG_nn'
Cuts at: 0 1715 2151 3021
Size: 1715 436 870
Fragments arranged by size:
1715 870 436
BsrGI T'GTAC_A
Cuts at: 0 1848 3021
Size: 1848 1173
BstBI TT'CG_AA
Cuts at: 0 1239 1647 3021
Size: 1239 408 1374
Fragments arranged by size:
1374 1239 408
BstEII G'GTnAC_C
Cuts at: 0 145 3021
Size: 145 2876
BstYI r'GATC_y
Cuts at: 0 167 307 939 1840 2360 2807 3021
Size: 167 140 632 901 520 447 214
Fragments arranged by size:
901 632 520 447 214 167 140
BtgI C'CryG_G
Cuts at: 0 534 897 3021
Size: 534 363 2124
Fragments arranged by size:
2124 534 363
BtsI GCAGTG_nn'
Cuts at: 0 134 3021
Size: 134 2887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 615 650 1107 1142 3021
Size: 615 35 457 35 1879
Fragments arranged by size:
1879 615 457 35 35
EaeI y'GGCC_r
Cuts at: 0 2424 3021
Size: 2424 597
EarI CTCTTCn'nnn_
Cuts at: 0 355 1214 3021
Size: 355 859 1807
Fragments arranged by size:
1807 859 355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2142 3021
Size: 2142 879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 574 1045 1166 1565 1987 2188 2356
Size: 298 276 471 121 399 422 201 168
Cuts at: 2356 2386 2419 2572 2641 2767 3021
Size: 30 33 153 69 126 254
Fragments arranged by size:
471 422 399 298 276 254 201 168
153 126 121 69 33 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1265 3021
Size: 1265 1756
EcoO109I rG'GnC_Cy
Cuts at: 0 374 2007 3021
Size: 374 1633 1014
Fragments arranged by size:
1633 1014 374
EcoRV GAT'ATC
Cuts at: 0 531 3021
Size: 531 2490
HaeII r_GCGC'y
Cuts at: 0 363 1101 2474 3021
Size: 363 738 1373 547
Fragments arranged by size:
1373 738 547 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 612 644 1417 1449 1520 1552
Size: 547 32 33 32 773 32 71 32
Cuts at: 1552 2425 2457 2799 2831 3021
Size: 873 32 342 32 190
Fragments arranged by size:
873 773 547 342 190 71 33 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 842 1142 3021
Size: 842 300 1879
Fragments arranged by size:
1879 842 300
HpaI GTT'AAC
Cuts at: 0 842 3021
Size: 842 2179
KasI G'GCGC_C
Cuts at: 0 2470 3021
Size: 2470 551
MfeI C'AATT_G
Cuts at: 0 830 2696 3021
Size: 830 1866 325
Fragments arranged by size:
1866 830 325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 1088 3021
Size: 992 96 1933
Fragments arranged by size:
1933 992 96
MscI TGG'CCA
Cuts at: 0 2426 3021
Size: 2426 595
MslI CAynn'nnrTG
Cuts at: 0 2545 2636 3021
Size: 2545 91 385
Fragments arranged by size:
2545 385 91
MspA1I CmG'CkG
Cuts at: 0 370 888 2230 3021
Size: 370 518 1342 791
Fragments arranged by size:
1342 791 518 370
NarI GG'CG_CC
Cuts at: 0 2471 3021
Size: 2471 550
NsiI A_TGCA'T
Cuts at: 0 338 3021
Size: 338 2683
NspI r_CATG'y
Cuts at: 0 1314 2071 2112 3021
Size: 1314 757 41 909
Fragments arranged by size:
1314 909 757 41
PasI CC'CwG_GG
Cuts at: 0 2976 3021
Size: 2976 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 385 3021
Size: 321 64 2636
Fragments arranged by size:
2636 321 64
PfoI T'CCnGG_A
Cuts at: 0 2003 2363 3021
Size: 2003 360 658
Fragments arranged by size:
2003 658 360
PpuMI rG'GwC_Cy
Cuts at: 0 374 2007 3021
Size: 374 1633 1014
Fragments arranged by size:
1633 1014 374
PsiI TTA'TAA
Cuts at: 0 781 3021
Size: 781 2240
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1325 1357 3021
Size: 1325 32 1664
Fragments arranged by size:
1664 1325 32
PvuII CAG'CTG
Cuts at: 0 370 2230 3021
Size: 370 1860 791
Fragments arranged by size:
1860 791 370
SanDI GG'GwC_CC
Cuts at: 0 374 3021
Size: 374 2647
SapI GCTCTTCn'nnn_
Cuts at: 0 1214 3021
Size: 1214 1807
SfcI C'TryA_G
Cuts at: 0 710 3021
Size: 710 2311
SfoI GGC'GCC
Cuts at: 0 2472 3021
Size: 2472 549
SmaI CCC'GGG
Cuts at: 0 2584 3021
Size: 2584 437
SnaBI TAC'GTA
Cuts at: 0 1467 3021
Size: 1467 1554
StyI C'CwwG_G
Cuts at: 0 379 1200 3021
Size: 379 821 1821
Fragments arranged by size:
1821 821 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1046 1238 3020 3021
Size: 1046 192 1782 1
Fragments arranged by size:
1782 1046 192 1
TatI w'GTAC_w
Cuts at: 0 1848 2438 3021
Size: 1848 590 583
Fragments arranged by size:
1848 590 583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 1021 1053 2345 2377 2545 2577
Size: 375 32 614 32 1292 32 168 32
Cuts at: 2577 3021
Size: 444
Fragments arranged by size:
1292 614 444 375 168 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 559 3021
Size: 559 2462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 3021
Size: 1419 1602
XmaI C'CCGG_G
Cuts at: 0 2582 3021
Size: 2582 439
XmnI GAAnn'nnTTC
Cuts at: 0 915 3021
Size: 915 2106
ZraI GAC'GTC
Cuts at: 0 1802 3021
Size: 1802 1219
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII ApaLI ApoI AvaI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglII
Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BsrDI BsrGI BstBI BstEII BstYI BtgI BtsI
CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI
MslI MspA1I NarI NsiI NspI PasI PflMI PfoI
PpuMI PsiI PsrI PvuII SanDI SapI SfcI SfoI
SmaI SnaBI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BglI BlpI BmrI BmtI BplI
BpuEI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EcoICRI EcoRI FalI FseI
FspI FspAI HindIII KpnI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SpeI SphI SrfI SspI StuI SwaI XbaI
XhoI