Restriction map for
pFN21AB4189
(Linear) (Six-base) MAPSORT of: KIBB4189.seq Check: 8187 from: 1 to: 2265
KIBB4189.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AccI GT'mk_AC
Cuts at: 0 404 2265
Size: 404 1861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 679 1394 1421 1475 1502 1583 1607
Size: 212 467 715 27 54 27 81 24
Cuts at: 1607 1691 2265
Size: 84 574
Fragments arranged by size:
715 574 467 212 84 81 54 27
27 24
AflIII A'CryG_T
Cuts at: 0 407 633 2265
Size: 407 226 1632
Fragments arranged by size:
1632 407 226
AlwNI CAG_nnn'CTG
Cuts at: 0 1159 2265
Size: 1159 1106
ApaI G_GGCC'C
Cuts at: 0 2210 2265
Size: 2210 55
ApoI r'AATT_y
Cuts at: 0 442 499 1315 1690 1801 2265
Size: 442 57 816 375 111 464
Fragments arranged by size:
816 464 442 375 111 57
AvaI C'yCGr_G
Cuts at: 0 121 1708 2265
Size: 121 1587 557
Fragments arranged by size:
1587 557 121
BamHI G'GATC_C
Cuts at: 0 960 2265
Size: 960 1305
BanI G'GyrC_C
Cuts at: 0 7 926 2265
Size: 7 919 1339
Fragments arranged by size:
1339 919 7
BanII G_rGCy'C
Cuts at: 0 2210 2265
Size: 2210 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 872 1522 1655 2265
Size: 392 480 650 133 610
Fragments arranged by size:
650 610 480 392 133
BciVI GTATCCnnnnn_n'
Cuts at: 0 1887 2265
Size: 1887 378
Bme1580I G_kGCm'C
Cuts at: 0 2210 2265
Size: 2210 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 503 1089 1860 2265
Size: 503 586 771 405
Fragments arranged by size:
771 586 503 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 2265
Size: 407 1858
Bpu10I CC'TnA_GC
Cuts at: 0 1346 2265
Size: 1346 919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1059 1223 2265
Size: 1059 164 1042
Fragments arranged by size:
1059 1042 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 741 1711 2265
Size: 741 970 554
Fragments arranged by size:
970 741 554
BsaAI yAC'GTr
Cuts at: 0 408 2265
Size: 408 1857
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 126 156 2265
Size: 126 30 2109
Fragments arranged by size:
2109 126 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1606 1903 2140 2265
Size: 1606 297 237 125
Fragments arranged by size:
1606 297 237 125
BseYI C'CCAG_C
Cuts at: 0 1216 2265
Size: 1216 1049
BsiHKAI G_wGCw'C
Cuts at: 0 1214 2265
Size: 1214 1051
BsmI GAATG_Cn'
Cuts at: 0 56 1239 2265
Size: 56 1183 1026
Fragments arranged by size:
1183 1026 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 62 539 2265
Size: 62 477 1726
Fragments arranged by size:
1726 477 62
Bsp1286I G_dGCh'C
Cuts at: 0 1214 2210 2265
Size: 1214 996 55
Fragments arranged by size:
1214 996 55
BspHI T'CATG_A
Cuts at: 0 1749 2265
Size: 1749 516
BsrBI CCG'CTC
Cuts at: 0 5 2265
Size: 5 2260
BsrDI GCAATG_nn'
Cuts at: 0 200 2265
Size: 200 2065
BsrFI r'CCGG_y
Cuts at: 0 100 2265
Size: 100 2165
BstBI TT'CG_AA
Cuts at: 0 886 2090 2265
Size: 886 1204 175
Fragments arranged by size:
1204 886 175
BstYI r'GATC_y
Cuts at: 0 483 960 1477 2265
Size: 483 477 517 788
Fragments arranged by size:
788 517 483 477
BstZ17I GTA'TAC
Cuts at: 0 405 2265
Size: 405 1860
Bsu36I CC'TnA_GG
Cuts at: 0 1127 1144 2265
Size: 1127 17 1121
Fragments arranged by size:
1127 1121 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2258 2265
Size: 2258 7
BtsI GCAGTG_nn'
Cuts at: 0 1025 2265
Size: 1025 1240
EaeI y'GGCC_r
Cuts at: 0 1014 2265
Size: 1014 1251
EarI CTCTTCn'nnn_
Cuts at: 0 1397 1478 1532 1583 1880 2015 2265
Size: 1397 81 54 51 297 135 250
Fragments arranged by size:
1397 297 250 135 81 54 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 119 2002 2265
Size: 119 1883 263
Fragments arranged by size:
1883 263 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 407 679 1394 1421 1475 1502 1583
Size: 212 195 272 715 27 54 27 81
Cuts at: 1583 1607 1691 2265
Size: 24 84 574
Fragments arranged by size:
715 574 272 212 195 84 81 54
27 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1552 1576 2265
Size: 1552 24 689
Fragments arranged by size:
1552 689 24
EcoO109I rG'GnC_Cy
Cuts at: 0 156 1183 2206 2207 2265
Size: 156 1027 1023 1 58
Fragments arranged by size:
1027 1023 156 58 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 553 2265
Size: 553 1712
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 126 158 229 261 782 814 1147 1179
Size: 126 32 71 32 521 32 333 32
Cuts at: 1179 1624 1656 1801 1833 1906 1938 2265
Size: 445 32 145 32 73 32 327
Fragments arranged by size:
521 445 333 327 145 126 73 71
32 32 32 32 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 2265
Size: 719 1546
MslI CAynn'nnrTG
Cuts at: 0 475 932 1754 2265
Size: 475 457 822 511
Fragments arranged by size:
822 511 475 457
MspA1I CmG'CkG
Cuts at: 0 1436 2265
Size: 1436 829
NaeI GCC'GGC
Cuts at: 0 102 2265
Size: 102 2163
NdeI CA'TA_TG
Cuts at: 0 1899 2265
Size: 1899 366
NgoMIV G'CCGG_C
Cuts at: 0 100 2265
Size: 100 2165
NspI r_CATG'y
Cuts at: 0 637 2265
Size: 637 1628
PciI A'CATG_T
Cuts at: 0 633 2265
Size: 633 1632
PfoI T'CCnGG_A
Cuts at: 0 772 2265
Size: 772 1493
PpuMI rG'GwC_Cy
Cuts at: 0 156 2265
Size: 156 2109
PspOMI G'GGCC_C
Cuts at: 0 2206 2265
Size: 2206 59
PstI C_TGCA'G
Cuts at: 0 291 2265
Size: 291 1974
PvuII CAG'CTG
Cuts at: 0 1436 2265
Size: 1436 829
SfcI C'TryA_G
Cuts at: 0 287 1049 1274 2265
Size: 287 762 225 991
Fragments arranged by size:
991 762 287 225
SmlI C'TyrA_G
Cuts at: 0 1074 1238 1708 2265
Size: 1074 164 470 557
Fragments arranged by size:
1074 557 470 164
StuI AGG'CCT
Cuts at: 0 1142 2265
Size: 1142 1123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 109 944 2170 2265
Size: 103 6 835 1226 95
Fragments arranged by size:
1226 835 103 95 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2166 2198 2265
Size: 2166 32 67
Fragments arranged by size:
2166 67 32
XbaI T'CTAG_A
Cuts at: 0 1189 2265
Size: 1189 1076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1039 2265
Size: 1039 1226
XhoI C'TCGA_G
Cuts at: 0 1708 2265
Size: 1708 557
XmnI GAAnn'nnTTC
Cuts at: 0 869 1757 2265
Size: 869 888 508
Fragments arranged by size:
888 869 508
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BciVI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BstBI BstYI
BstZ17I Bsu36I BtgZI BtsI EaeI EarI EciI Eco57MI
EcoNI EcoO109I FalI Hin4I MmeI MslI MspA1I NaeI
NdeI NgoMIV NspI PciI PfoI PpuMI PspOMI PstI
PvuII SfcI SmlI StuI TaqII TstI XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BspEI BspMI BsrGI BssHII BssSI BstAPI
BstEII BstXI BtgI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NarI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI Tth111I
XmaI ZraI