Restriction map for
pFN21AB3917
(Linear) (Six-base) MAPSORT of: KIBB3917.seq Check: 1471 from: 1 to: 5160
KIBB3917.orf
With 182 enzymes: SgfI *
March 4, 2015 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 205 5160
Size: 205 4955
AatII G_ACGT'C
Cuts at: 0 3896 5160
Size: 3896 1264
AccI GT'mk_AC
Cuts at: 0 404 4907 5160
Size: 404 4503 253
Fragments arranged by size:
4503 404 253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 195 1165 1730 2047 2608 3189 3613
Size: 86 109 970 565 317 561 581 424
Cuts at: 3613 3844 3862 4229 5062 5160
Size: 231 18 367 833 98
Fragments arranged by size:
970 833 581 565 561 424 367 317
231 109 98 86 18
AfeI AGC'GCT
Cuts at: 0 610 1524 3604 5160
Size: 610 914 2080 1556
Fragments arranged by size:
2080 1556 914 610
AflIII A'CryG_T
Cuts at: 0 537 548 1343 4829 5160
Size: 537 11 795 3486 331
Fragments arranged by size:
3486 795 537 331 11
AleI CACnn'nnGTG
Cuts at: 0 199 2935 3859 5160
Size: 199 2736 924 1301
Fragments arranged by size:
2736 1301 924 199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2818 3058 5160
Size: 2818 240 2102
Fragments arranged by size:
2818 2102 240
AlwNI CAG_nnn'CTG
Cuts at: 0 218 1260 1566 3051 3093 3963 5040 5154
Size: 218 1042 306 1485 42 870 1077 114
Cuts at: 5154 5160
Size: 6
Fragments arranged by size:
1485 1077 1042 870 306 218 114 42
6
ApaI G_GGCC'C
Cuts at: 0 169 393 2471 4484 5160
Size: 169 224 2078 2013 676
Fragments arranged by size:
2078 2013 676 224 169
ApaLI G'TGCA_C
Cuts at: 0 691 1468 5160
Size: 691 777 3692
Fragments arranged by size:
3692 777 691
ApoI r'AATT_y
Cuts at: 0 419 467 1370 3284 3931 5160
Size: 419 48 903 1914 647 1229
Fragments arranged by size:
1914 1229 903 647 419 48
AvaI C'yCGr_G
Cuts at: 0 338 1928 1946 4003 4483 5160
Size: 338 1590 18 2057 480 677
Fragments arranged by size:
2057 1590 677 480 338 18
AvrII C'CTAG_G
Cuts at: 0 942 5160
Size: 942 4218
BamHI G'GATC_C
Cuts at: 0 4982 5062 5160
Size: 4982 80 98
Fragments arranged by size:
4982 98 80
BanI G'GyrC_C
Cuts at: 0 227 909 1584 3259 4571 4648 4682 5160
Size: 227 682 675 1675 1312 77 34 478
Fragments arranged by size:
1675 1312 682 675 478 227 77 34
BanII G_rGCy'C
Cuts at: 0 74 169 248 307 393 725 2269 2471
Size: 74 95 79 59 86 332 1544 202
Cuts at: 2471 3665 4475 4484 4624 5144 5160
Size: 1194 810 9 140 520 16
Fragments arranged by size:
1544 1194 810 520 332 202 140 95
86 79 74 59 16 9
BbeI G_GCGC'C
Cuts at: 0 3263 5160
Size: 3263 1897
BbsI GAAGACnn'nnnn_
Cuts at: 0 2405 4334 5160
Size: 2405 1929 826
Fragments arranged by size:
2405 1929 826
BbvCI CC'TCA_GC
Cuts at: 0 370 3361 4214 5077 5160
Size: 370 2991 853 863 83
Fragments arranged by size:
2991 863 853 370 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 30 64 439 473 1765 1799 4887 4921
Size: 30 34 375 34 1292 34 3088 34
Cuts at: 4921 5160
Size: 239
Fragments arranged by size:
3088 1292 375 239 34 34 34 34
30
BciVI GTATCCnnnnn_n'
Cuts at: 0 754 5160
Size: 754 4406
BclI T'GATC_A
Cuts at: 0 1842 2210 5160
Size: 1842 368 2950
Fragments arranged by size:
2950 1842 368
BfrBI ATG'CAT
Cuts at: 0 2639 3973 5160
Size: 2639 1334 1187
Fragments arranged by size:
2639 1334 1187
BglI GCCn_nnn'nGGC
Cuts at: 0 27 1582 2747 2954 4479 5160
Size: 27 1555 1165 207 1525 681
Fragments arranged by size:
1555 1525 1165 681 207 27
BglII A'GATC_T
Cuts at: 0 2300 2732 5160
Size: 2300 432 2428
Fragments arranged by size:
2428 2300 432
BlpI GC'TnA_GC
Cuts at: 0 605 5160
Size: 605 4555
Bme1580I G_kGCm'C
Cuts at: 0 169 230 393 695 783 1472 1587 1698
Size: 169 61 163 302 88 689 115 111
Cuts at: 1698 2471 3800 4196 4484 5160
Size: 773 1329 396 288 676
Fragments arranged by size:
1329 773 689 676 396 302 288 169
163 115 111 88 61
BmgBI CAC'GTC
Cuts at: 0 1191 5160
Size: 1191 3969
BmrI ACTGGGnnnn_n'
Cuts at: 0 875 925 2144 3428 5160
Size: 875 50 1219 1284 1732
Fragments arranged by size:
1732 1284 1219 875 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 5160
Size: 207 4953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 5160
Size: 1282 3878
Bpu10I CC'TnA_GC
Cuts at: 0 370 1519 1589 2952 3361 4214 4401 5077
Size: 370 1149 70 1363 409 853 187 676
Cuts at: 5077 5160
Size: 83
Fragments arranged by size:
1363 1149 853 676 409 370 187 83
70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 433 903 3288 3334 5128 5160
Size: 58 375 470 2385 46 1794 32
Fragments arranged by size:
2385 1794 470 375 58 46 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 941 2308 2766 3993 5087 5160
Size: 941 1367 458 1227 1094 73
Fragments arranged by size:
1367 1227 1094 941 458 73
BsaAI yAC'GTr
Cuts at: 0 4832 5160
Size: 4832 328
BsaBI GATnn'nnATC
Cuts at: 0 2546 2731 4603 5160
Size: 2546 185 1872 557
Fragments arranged by size:
2546 1872 557 185
BsaHI Gr'CG_yC
Cuts at: 0 2788 3260 3450 3893 4226 5160
Size: 2788 472 190 443 333 934
Fragments arranged by size:
2788 934 472 443 333 190
BsaWI w'CCGG_w
Cuts at: 0 4985 5160
Size: 4985 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1660 1690 1954 1984 3140 3170 4405 4435
Size: 1660 30 264 30 1156 30 1235 30
Cuts at: 4435 4552 4582 4606 4636 5160
Size: 117 30 24 30 524
Fragments arranged by size:
1660 1235 1156 524 264 117 30 30
30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 2150 4167 4201 4231 4279 4282 4309
Size: 262 1888 2017 34 30 48 3 27
Cuts at: 4309 4312 4327 4426 4573 5160
Size: 3 15 99 147 587
Fragments arranged by size:
2017 1888 587 262 147 99 48 34
30 27 15 3 3
BseYI C'CCAG_C
Cuts at: 0 1538 1572 1806 2745 4138 5058 5160
Size: 1538 34 234 939 1393 920 102
Fragments arranged by size:
1538 1393 939 920 234 102 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 526 1651 1821 2713 3022 3064 5160
Size: 223 303 1125 170 892 309 42 2096
Fragments arranged by size:
2096 1125 892 309 303 223 170 42
BsiEI CG_ry'CG
Cuts at: 0 3419 4907 5160
Size: 3419 1488 253
Fragments arranged by size:
3419 1488 253
BsiHKAI G_wGCw'C
Cuts at: 0 74 193 248 695 1472 1955 2269 3806
Size: 74 119 55 447 777 483 314 1537
Cuts at: 3806 4368 4624 5144 5160
Size: 562 256 520 16
Fragments arranged by size:
1537 777 562 520 483 447 314 256
119 74 55 16
BsmI GAATG_Cn'
Cuts at: 0 606 1788 3731 5160
Size: 606 1182 1943 1429
Fragments arranged by size:
1943 1429 1182 606
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1766 5160
Size: 1766 3394
Bsp1286I G_dGCh'C
Cuts at: 0 74 169 193 230 248 307 393 695
Size: 74 95 24 37 18 59 86 302
Cuts at: 695 725 783 1472 1587 1698 1955 2269 2471
Size: 30 58 689 115 111 257 314 202
Cuts at: 2471 3665 3800 3806 4196 4368 4475 4484 4624
Size: 1194 135 6 390 172 107 9 140
Cuts at: 4624 5144 5160
Size: 520 16
Fragments arranged by size:
1194 689 520 390 314 302 257 202
172 140 135 115 111 107 95 86
74 59 58 37 30 24 18 16
9 6
BspHI T'CATG_A
Cuts at: 0 992 5160
Size: 992 4168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 205 299 2344 2372 2504 2849 5160
Size: 205 94 2045 28 132 345 2311
Fragments arranged by size:
2311 2045 345 205 132 94 28
BsrBI CCG'CTC
Cuts at: 0 2951 5160
Size: 2951 2209
BsrDI GCAATG_nn'
Cuts at: 0 572 2625 3336 5160
Size: 572 2053 711 1824
Fragments arranged by size:
2053 1824 711 572
BsrFI r'CCGG_y
Cuts at: 0 1616 2869 5160
Size: 1616 1253 2291
Fragments arranged by size:
2291 1616 1253
BsrGI T'GTAC_A
Cuts at: 0 1346 5160
Size: 1346 3814
BssHII G'CGCG_C
Cuts at: 0 1321 5160
Size: 1321 3839
BssSI C'ACGA_G
Cuts at: 0 2736 5160
Size: 2736 2424
BstBI TT'CG_AA
Cuts at: 0 1014 5160
Size: 1014 4146
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5058 5160
Size: 5058 102
BstYI r'GATC_y
Cuts at: 0 1256 2300 2732 3180 4125 4520 4785 4982
Size: 1256 1044 432 448 945 395 265 197
Cuts at: 4982 5062 5160
Size: 80 98
Fragments arranged by size:
1256 1044 945 448 432 395 265 197
98 80
BtgI C'CryG_G
Cuts at: 0 686 1376 1420 1484 1550 1577 4490 4644
Size: 686 690 44 64 66 27 2913 154
Cuts at: 4644 5160
Size: 516
Fragments arranged by size:
2913 690 686 516 154 66 64 44
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4884 5160
Size: 4884 276
BtsI GCAGTG_nn'
Cuts at: 0 618 1663 2286 2460 2800 2898 2930 3042
Size: 618 1045 623 174 340 98 32 112
Cuts at: 3042 5160
Size: 2118
Fragments arranged by size:
2118 1045 623 618 340 174 112 98
32
DraI TTT'AAA
Cuts at: 0 4041 5160
Size: 4041 1119
DraIII CAC_nnn'GTG
Cuts at: 0 3613 4200 4682 5160
Size: 3613 587 482 478
Fragments arranged by size:
3613 587 482 478
EaeI y'GGCC_r
Cuts at: 0 705 966 1064 1553 1869 3119 3333 3416
Size: 705 261 98 489 316 1250 214 83
Cuts at: 3416 3761 5160
Size: 345 1399
Fragments arranged by size:
1399 1250 705 489 345 316 261 214
98 83
EagI C'GGCC_G
Cuts at: 0 3416 5160
Size: 3416 1744
EarI CTCTTCn'nnn_
Cuts at: 0 62 280 586 1706 2356 3249 3532 3820
Size: 62 218 306 1120 650 893 283 288
Cuts at: 3820 4104 4178 4286 4385 5001 5160
Size: 284 74 108 99 616 159
Fragments arranged by size:
1120 893 650 616 306 288 284 283
218 159 108 99 74 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2259 5160
Size: 2259 2901
EcoICRI GAG'CTC
Cuts at: 0 72 246 2267 4622 5142 5160
Size: 72 174 2021 2355 520 18
Fragments arranged by size:
2355 2021 520 174 72 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 195 1165 1282 1730 2047 2608 3189
Size: 86 109 970 117 448 317 561 581
Cuts at: 3189 3613 3844 3862 4229 5062 5160
Size: 424 231 18 367 833 98
Fragments arranged by size:
970 833 581 561 448 424 367 317
231 117 109 98 86 18
EcoO109I rG'GnC_Cy
Cuts at: 0 166 389 390 524 2468 2919 3578 4416
Size: 166 223 1 134 1944 451 659 838
Cuts at: 4416 4480 4779 5160
Size: 64 299 381
Fragments arranged by size:
1944 838 659 451 381 299 223 166
134 64 1
EcoRI G'AATT_C
Cuts at: 0 419 1370 5160
Size: 419 951 3790
Fragments arranged by size:
3790 951 419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4626 5160
Size: 4626 534
HaeII r_GCGC'y
Cuts at: 0 612 1526 3263 3606 3770 4500 5160
Size: 612 914 1737 343 164 730 660
Fragments arranged by size:
1737 914 730 660 612 343 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 626 658 1664 1696 1881 1913 4405 4437
Size: 626 32 1006 32 185 32 2492 32
Cuts at: 4437 4606 4638 5065 5097 5160
Size: 169 32 427 32 63
Fragments arranged by size:
2492 1006 626 427 185 169 63 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 668 2066 4908 5160
Size: 668 1398 2842 252
Fragments arranged by size:
2842 1398 668 252
KasI G'GCGC_C
Cuts at: 0 3259 5160
Size: 3259 1901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2749 3359 4829 5160
Size: 2749 610 1470 331
Fragments arranged by size:
2749 1470 610 331
MscI TGG'CCA
Cuts at: 0 707 968 1066 1555 1871 3121 3763 5160
Size: 707 261 98 489 316 1250 642 1397
Fragments arranged by size:
1397 1250 707 642 489 316 261 98
MslI CAynn'nnrTG
Cuts at: 0 199 599 2407 2935 3158 3673 3859 5160
Size: 199 400 1808 528 223 515 186 1301
Fragments arranged by size:
1808 1301 528 515 400 223 199 186
MspA1I CmG'CkG
Cuts at: 0 369 703 2040 2321 2781 3591 3948 4122
Size: 369 334 1337 281 460 810 357 174
Cuts at: 4122 5160
Size: 1038
Fragments arranged by size:
1337 1038 810 460 369 357 334 281
174
NaeI GCC'GGC
Cuts at: 0 1618 5160
Size: 1618 3542
NarI GG'CG_CC
Cuts at: 0 3260 5160
Size: 3260 1900
NcoI C'CATG_G
Cuts at: 0 686 1376 1420 1484 4490 4644 5160
Size: 686 690 44 64 3006 154 516
Fragments arranged by size:
3006 690 686 516 154 64 44
NdeI CA'TA_TG
Cuts at: 0 596 2514 5160
Size: 596 1918 2646
Fragments arranged by size:
2646 1918 596
NgoMIV G'CCGG_C
Cuts at: 0 1616 5160
Size: 1616 3544
NotI GC'GGCC_GC
Cuts at: 0 3416 5160
Size: 3416 1744
NsiI A_TGCA'T
Cuts at: 0 2641 3975 5160
Size: 2641 1334 1185
Fragments arranged by size:
2641 1334 1185
NspI r_CATG'y
Cuts at: 0 21 327 541 1347 1624 2639 2643 3973
Size: 21 306 214 806 277 1015 4 1330
Cuts at: 3973 4335 5160
Size: 362 825
Fragments arranged by size:
1330 1015 825 806 362 306 277 214
21 4
PasI CC'CwG_GG
Cuts at: 0 528 726 1596 2834 3343 3726 4476 5160
Size: 528 198 870 1238 509 383 750 684
Fragments arranged by size:
1238 870 750 684 528 509 383 198
PciI A'CATG_T
Cuts at: 0 537 1343 5160
Size: 537 806 3817
Fragments arranged by size:
3817 806 537
PflMI CCAn_nnn'nTGG
Cuts at: 0 1878 2904 5160
Size: 1878 1026 2256
Fragments arranged by size:
2256 1878 1026
PfoI T'CCnGG_A
Cuts at: 0 1259 1892 3896 4781 5160
Size: 1259 633 2004 885 379
Fragments arranged by size:
2004 1259 885 633 379
PpuMI rG'GwC_Cy
Cuts at: 0 524 3578 4416 4779 5160
Size: 524 3054 838 363 381
Fragments arranged by size:
3054 838 524 381 363
PshAI GACnn'nnGTC
Cuts at: 0 3434 3509 5160
Size: 3434 75 1651
Fragments arranged by size:
3434 1651 75
PspOMI G'GGCC_C
Cuts at: 0 165 389 2467 4480 5160
Size: 165 224 2078 2013 680
Fragments arranged by size:
2078 2013 680 224 165
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 94 126 5160
Size: 94 32 5034
Fragments arranged by size:
5034 94 32
PstI C_TGCA'G
Cuts at: 0 38 174 2369 2684 3098 3590 5160
Size: 38 136 2195 315 414 492 1570
Fragments arranged by size:
2195 1570 492 414 315 136 38
PvuII CAG'CTG
Cuts at: 0 369 703 3591 3948 5160
Size: 369 334 2888 357 1212
Fragments arranged by size:
2888 1212 369 357 334
RsrII CG'GwC_CG
Cuts at: 0 3018 5160
Size: 3018 2142
SacI G_AGCT'C
Cuts at: 0 74 248 2269 4624 5144 5160
Size: 74 174 2021 2355 520 16
Fragments arranged by size:
2355 2021 520 174 74 16
SalI G'TCGA_C
Cuts at: 0 4906 5160
Size: 4906 254
SanDI GG'GwC_CC
Cuts at: 0 524 5160
Size: 524 4636
SapI GCTCTTCn'nnn_
Cuts at: 0 62 4104 5160
Size: 62 4042 1056
Fragments arranged by size:
4042 1056 62
SfcI C'TryA_G
Cuts at: 0 34 170 1076 2365 2373 2680 3094 3507
Size: 34 136 906 1289 8 307 414 413
Cuts at: 3507 3586 5005 5160
Size: 79 1419 155
Fragments arranged by size:
1419 1289 906 414 413 307 155 136
79 34 8
SfoI GGC'GCC
Cuts at: 0 3261 5160
Size: 3261 1899
SmaI CCC'GGG
Cuts at: 0 340 1948 5160
Size: 340 1608 3212
Fragments arranged by size:
3212 1608 340
SmlI C'TyrA_G
Cuts at: 0 73 412 918 3267 3313 5143 5160
Size: 73 339 506 2349 46 1830 17
Fragments arranged by size:
2349 1830 506 339 73 46 17
SphI G_CATG'C
Cuts at: 0 2639 2643 5160
Size: 2639 4 2517
Fragments arranged by size:
2639 2517 4
SspI AAT'ATT
Cuts at: 0 1197 5160
Size: 1197 3963
StuI AGG'CCT
Cuts at: 0 1277 1750 1822 5132 5160
Size: 1277 473 72 3310 28
Fragments arranged by size:
3310 1277 473 72 28
StyI C'CwwG_G
Cuts at: 0 686 942 1376 1420 1484 1862 2387 3736
Size: 686 256 434 44 64 378 525 1349
Cuts at: 3736 4490 4644 4667 5160
Size: 754 154 23 493
Fragments arranged by size:
1349 754 686 525 493 434 378 256
154 64 44 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 786 1086 1127 1487 2136 2473 3685 3798
Size: 786 300 41 360 649 337 1212 113
Cuts at: 3798 4474 5160
Size: 676 686
Fragments arranged by size:
1212 786 686 676 649 360 337 300
113 41
TatI w'GTAC_w
Cuts at: 0 1126 1346 5160
Size: 1126 220 3814
Fragments arranged by size:
3814 1126 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1292 1324 1660 1692 3140 3172 4552 4584
Size: 1292 32 336 32 1448 32 1380 32
Cuts at: 4584 5160
Size: 576
Fragments arranged by size:
1448 1380 1292 576 336 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 894 5160
Size: 894 4266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1418 3864 5160
Size: 1418 2446 1296
Fragments arranged by size:
2446 1418 1296
XmaI C'CCGG_G
Cuts at: 0 338 1946 5160
Size: 338 1608 3214
Fragments arranged by size:
3214 1608 338
XmnI GAAnn'nnTTC
Cuts at: 0 3931 5160
Size: 3931 1229
ZraI GAC'GTC
Cuts at: 0 3894 5160
Size: 3894 1266
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NsiI
NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI
PsrI PstI PvuII RsrII SacI SalI SanDI SapI
SfcI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AloI AscI AseI
AsiSI BaeI BmtI BsiWI BspEI BstAPI BstEII BstZ17I
Bsu36I ClaI CspCI DrdI EcoNI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NheI NruI
PacI PmeI PmlI PpiI PsiI PspXI PvuI SacII
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SwaI Tth111I XhoI