Restriction map for
pFN21AB3323
(Linear) (Six-base) MAPSORT of: KIBB3323.seq Check: 2207 from: 1 to: 1506
KIBB3323.orf
With 182 enzymes: SgfI *
February 5, 2013 10:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 580 1506
Size: 308 272 926
Fragments arranged by size:
926 308 272
AleI CACnn'nnGTG
Cuts at: 0 913 943 1506
Size: 913 30 563
Fragments arranged by size:
913 563 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 829 1450 1506
Size: 829 621 56
Fragments arranged by size:
829 621 56
AlwNI CAG_nnn'CTG
Cuts at: 0 1080 1506
Size: 1080 426
ApaI G_GGCC'C
Cuts at: 0 1237 1506
Size: 1237 269
ApaLI G'TGCA_C
Cuts at: 0 961 1506
Size: 961 545
AvaI C'yCGr_G
Cuts at: 0 1237 1506
Size: 1237 269
BamHI G'GATC_C
Cuts at: 0 111 1506
Size: 111 1395
BanI G'GyrC_C
Cuts at: 0 993 1417 1506
Size: 993 424 89
Fragments arranged by size:
993 424 89
BanII G_rGCy'C
Cuts at: 0 1125 1237 1506
Size: 1125 112 269
Fragments arranged by size:
1125 269 112
BbeI G_GCGC'C
Cuts at: 0 997 1506
Size: 997 509
BbsI GAAGACnn'nnnn_
Cuts at: 0 756 825 1005 1506
Size: 756 69 180 501
Fragments arranged by size:
756 501 180 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 528 562 1149 1183 1506
Size: 528 34 587 34 323
Fragments arranged by size:
587 528 323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 72 1506
Size: 72 1434
BglI GCCn_nnn'nGGC
Cuts at: 0 630 1147 1210 1506
Size: 630 517 63 296
Fragments arranged by size:
630 517 296 63
BlpI GC'TnA_GC
Cuts at: 0 1214 1506
Size: 1214 292
Bme1580I G_kGCm'C
Cuts at: 0 965 1237 1506
Size: 965 272 269
Fragments arranged by size:
965 272 269
BmgBI CAC'GTC
Cuts at: 0 879 1506
Size: 879 627
BmtI G_CTAG'C
Cuts at: 0 754 1506
Size: 754 752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1139 1506
Size: 1039 100 367
Fragments arranged by size:
1039 367 100
Bpu10I CC'TnA_GC
Cuts at: 0 60 1245 1506
Size: 60 1185 261
Fragments arranged by size:
1185 261 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 1506
Size: 414 1092
BsaAI yAC'GTr
Cuts at: 0 659 663 674 1163 1506
Size: 659 4 11 489 343
Fragments arranged by size:
659 489 343 11 4
BsaHI Gr'CG_yC
Cuts at: 0 994 1095 1506
Size: 994 101 411
Fragments arranged by size:
994 411 101
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 100 1506
Size: 37 63 1406
Fragments arranged by size:
1406 63 37
BseYI C'CCAG_C
Cuts at: 0 141 194 1495 1506
Size: 141 53 1301 11
Fragments arranged by size:
1301 141 53 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1143 1205 1207 1506
Size: 489 654 62 2 299
Fragments arranged by size:
654 489 299 62 2
BsiEI CG_ry'CG
Cuts at: 0 537 1377 1506
Size: 537 840 129
Fragments arranged by size:
840 537 129
BsiHKAI G_wGCw'C
Cuts at: 0 61 740 956 965 1506
Size: 61 679 216 9 541
Fragments arranged by size:
679 541 216 61 9
BsiWI C'GTAC_G
Cuts at: 0 659 1506
Size: 659 847
Bsp1286I G_dGCh'C
Cuts at: 0 61 740 956 965 1125 1237 1506
Size: 61 679 216 9 160 112 269
Fragments arranged by size:
679 269 216 160 112 61 9
BspHI T'CATG_A
Cuts at: 0 227 1506
Size: 227 1279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 779 1506
Size: 779 727
BsrBI CCG'CTC
Cuts at: 0 90 216 702 885 971 1506
Size: 90 126 486 183 86 535
Fragments arranged by size:
535 486 183 126 90 86
BsrDI GCAATG_nn'
Cuts at: 0 1062 1506
Size: 1062 444
BsrFI r'CCGG_y
Cuts at: 0 629 1506
Size: 629 877
BsrGI T'GTAC_A
Cuts at: 0 356 1506
Size: 356 1150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1506
Size: 405 1101
BstYI r'GATC_y
Cuts at: 0 111 1391 1506
Size: 111 1280 115
Fragments arranged by size:
1280 115 111
BtgI C'CryG_G
Cuts at: 0 20 404 911 989 1364 1506
Size: 20 384 507 78 375 142
Fragments arranged by size:
507 384 375 142 78 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1284 1506
Size: 1284 222
DraIII CAC_nnn'GTG
Cuts at: 0 598 1506
Size: 598 908
EaeI y'GGCC_r
Cuts at: 0 572 1196 1506
Size: 572 624 310
Fragments arranged by size:
624 572 310
EarI CTCTTCn'nnn_
Cuts at: 0 284 556 709 1045 1132 1387 1450 1506
Size: 284 272 153 336 87 255 63 56
Fragments arranged by size:
336 284 272 255 153 87 63 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 1506
Size: 127 1379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 580 1039 1139 1506
Size: 308 272 459 100 367
Fragments arranged by size:
459 367 308 272 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1273 1506
Size: 1273 233
EcoO109I rG'GnC_Cy
Cuts at: 0 1202 1234 1506
Size: 1202 32 272
Fragments arranged by size:
1202 272 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 720 1029 1506
Size: 720 309 477
Fragments arranged by size:
720 477 309
HaeII r_GCGC'y
Cuts at: 0 12 28 622 826 997 1080 1118 1245
Size: 12 16 594 204 171 83 38 127
Cuts at: 1245 1506
Size: 261
Fragments arranged by size:
594 261 204 171 127 83 38 16
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 846 878 1506
Size: 846 32 628
Fragments arranged by size:
846 628 32
HincII GTy'rAC
Cuts at: 0 1173 1506
Size: 1173 333
HindIII A'AGCT_T
Cuts at: 0 130 1506
Size: 130 1376
KasI G'GCGC_C
Cuts at: 0 993 1506
Size: 993 513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1506
Size: 803 703
MscI TGG'CCA
Cuts at: 0 574 1506
Size: 574 932
MslI CAynn'nnrTG
Cuts at: 0 205 811 913 943 1426 1506
Size: 205 606 102 30 483 80
Fragments arranged by size:
606 483 205 102 80 30
NaeI GCC'GGC
Cuts at: 0 631 1506
Size: 631 875
NarI GG'CG_CC
Cuts at: 0 994 1506
Size: 994 512
NcoI C'CATG_G
Cuts at: 0 911 989 1364 1506
Size: 911 78 375 142
Fragments arranged by size:
911 375 142 78
NgoMIV G'CCGG_C
Cuts at: 0 629 1506
Size: 629 877
NheI G'CTAG_C
Cuts at: 0 750 1506
Size: 750 756
NspI r_CATG'y
Cuts at: 0 378 464 1314 1431 1506
Size: 378 86 850 117 75
Fragments arranged by size:
850 378 117 86 75
PasI CC'CwG_GG
Cuts at: 0 998 1144 1506
Size: 998 146 362
Fragments arranged by size:
998 362 146
PflMI CCAn_nnn'nTGG
Cuts at: 0 242 1506
Size: 242 1264
PfoI T'CCnGG_A
Cuts at: 0 107 1016 1271 1506
Size: 107 909 255 235
Fragments arranged by size:
909 255 235 107
PmlI CAC'GTG
Cuts at: 0 1163 1506
Size: 1163 343
PspOMI G'GGCC_C
Cuts at: 0 1233 1506
Size: 1233 273
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 889 921 1506
Size: 889 32 585
Fragments arranged by size:
889 585 32
PstI C_TGCA'G
Cuts at: 0 1439 1506
Size: 1439 67
SapI GCTCTTCn'nnn_
Cuts at: 0 709 1045 1506
Size: 709 336 461
Fragments arranged by size:
709 461 336
SfcI C'TryA_G
Cuts at: 0 1435 1506
Size: 1435 71
SfoI GGC'GCC
Cuts at: 0 995 1506
Size: 995 511
SmaI CCC'GGG
Cuts at: 0 1239 1506
Size: 1239 267
SnaBI TAC'GTA
Cuts at: 0 659 1506
Size: 659 847
SphI G_CATG'C
Cuts at: 0 464 1506
Size: 464 1042
StyI C'CwwG_G
Cuts at: 0 33 911 989 1364 1506
Size: 33 878 78 375 142
Fragments arranged by size:
878 375 142 78 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 990 1506
Size: 990 516
TatI w'GTAC_w
Cuts at: 0 171 356 1506
Size: 171 185 1150
Fragments arranged by size:
1150 185 171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 987 1506
Size: 987 519
XmaI C'CCGG_G
Cuts at: 0 1237 1506
Size: 1237 269
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI BtgI
BtgZI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI NaeI NarI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PmlI PspOMI PsrI PstI SapI SfcI
SfoI SmaI SnaBI SphI StyI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbvCI BclI BfrBI BglII BmrI BplI BpuEI
BsaBI BsaWI BsaXI BsmI BsmBI BspEI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI HpaI KpnI MfeI MluI MspA1I NdeI NotI
NruI NsiI PacI PciI PmeI PpiI PpuMI PshAI
PsiI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI