Restriction map for
pFN21AB3260
(Linear) (Six-base) MAPSORT of: KIBB3260.seq Check: 6459 from: 1 to: 4845
KIBB3260.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1092 3781 4845
Size: 1092 2689 1064
Fragments arranged by size:
2689 1092 1064
AatII G_ACGT'C
Cuts at: 0 1739 2081 4845
Size: 1739 342 2764
Fragments arranged by size:
2764 1739 342
AccI GT'mk_AC
Cuts at: 0 1160 1268 3356 4845
Size: 1160 108 2088 1489
Fragments arranged by size:
2088 1489 1160 108
Acc65I G'GTAC_C
Cuts at: 0 3236 4845
Size: 3236 1609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2107 3418 4845
Size: 2107 1311 1427
Fragments arranged by size:
2107 1427 1311
AfeI AGC'GCT
Cuts at: 0 4240 4845
Size: 4240 605
AflIII A'CryG_T
Cuts at: 0 491 1003 4541 4637 4845
Size: 491 512 3538 96 208
Fragments arranged by size:
3538 512 491 208 96
AleI CACnn'nnGTG
Cuts at: 0 169 976 3647 3708 4151 4636 4845
Size: 169 807 2671 61 443 485 209
Fragments arranged by size:
2671 807 485 443 209 169 61
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1142 4845
Size: 1142 3703
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 664 696 2233 2265 4845
Size: 664 32 1537 32 2580
Fragments arranged by size:
2580 1537 664 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 858 1227 3471 3747 4667 4749 4845
Size: 858 369 2244 276 920 82 96
Fragments arranged by size:
2244 920 858 369 276 96 82
ApaI G_GGCC'C
Cuts at: 0 4166 4262 4845
Size: 4166 96 583
Fragments arranged by size:
4166 583 96
ApaLI G'TGCA_C
Cuts at: 0 1711 4845
Size: 1711 3134
AvaI C'yCGr_G
Cuts at: 0 1382 1504 1978 2158 2286 3823 3850 4317
Size: 1382 122 474 180 128 1537 27 467
Cuts at: 4317 4845
Size: 528
Fragments arranged by size:
1537 1382 528 474 467 180 128 122
27
BamHI G'GATC_C
Cuts at: 0 2304 4845
Size: 2304 2541
BanI G'GyrC_C
Cuts at: 0 1339 2987 3236 3298 4407 4608 4845
Size: 1339 1648 249 62 1109 201 237
Fragments arranged by size:
1648 1339 1109 249 237 201 62
BanII G_rGCy'C
Cuts at: 0 1382 1772 2122 4103 4166 4262 4378 4845
Size: 1382 390 350 1981 63 96 116 467
Fragments arranged by size:
1981 1382 467 390 350 116 96 63
BbeI G_GCGC'C
Cuts at: 0 2991 4612 4845
Size: 2991 1621 233
Fragments arranged by size:
2991 1621 233
BbsI GAAGACnn'nnnn_
Cuts at: 0 1918 1935 2095 3550 4845
Size: 1918 17 160 1455 1295
Fragments arranged by size:
1918 1455 1295 160 17
BbvCI CC'TCA_GC
Cuts at: 0 2031 3085 4845
Size: 2031 1054 1760
Fragments arranged by size:
2031 1760 1054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1248 1282 2340 2374 4140 4174 4845
Size: 1248 34 1058 34 1766 34 671
Fragments arranged by size:
1766 1248 1058 671 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3829 4845
Size: 3829 1016
BclI T'GATC_A
Cuts at: 0 1586 4845
Size: 1586 3259
BglI GCCn_nnn'nGGC
Cuts at: 0 1337 1886 2702 4845
Size: 1337 549 816 2143
Fragments arranged by size:
2143 1337 816 549
BlpI GC'TnA_GC
Cuts at: 0 646 859 3157 4845
Size: 646 213 2298 1688
Fragments arranged by size:
2298 1688 646 213
Bme1580I G_kGCm'C
Cuts at: 0 831 1309 1715 3654 4166 4262 4301 4412
Size: 831 478 406 1939 512 96 39 111
Cuts at: 4412 4845
Size: 433
Fragments arranged by size:
1939 831 512 478 433 406 111 96
39
BmgBI CAC'GTC
Cuts at: 0 243 1004 1998 3122 4431 4638 4845
Size: 243 761 994 1124 1309 207 207
Fragments arranged by size:
1309 1124 994 761 243 207 207
BmrI ACTGGGnnnn_n'
Cuts at: 0 357 516 639 809 1281 1440 1563 2208
Size: 357 159 123 170 472 159 123 645
Cuts at: 2208 2219 2985 3376 3715 3897 4845
Size: 11 766 391 339 182 948
Fragments arranged by size:
948 766 645 472 391 357 339 182
170 159 159 123 123 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3651 4845
Size: 3651 1194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 205 631 1642 1912 3466 3646 4845
Size: 178 27 426 1011 270 1554 180 1199
Fragments arranged by size:
1554 1199 1011 426 270 180 178 27
Bpu10I CC'TnA_GC
Cuts at: 0 2031 3085 4845
Size: 2031 1054 1760
Fragments arranged by size:
2031 1760 1054
BsaI GGTCTCn'nnnn_
Cuts at: 0 327 376 1975 4569 4845
Size: 327 49 1599 2594 276
Fragments arranged by size:
2594 1599 327 276 49
BsaAI yAC'GTr
Cuts at: 0 2133 4296 4845
Size: 2133 2163 549
Fragments arranged by size:
2163 2133 549
BsaBI GATnn'nnATC
Cuts at: 0 2671 4845
Size: 2671 2174
BsaHI Gr'CG_yC
Cuts at: 0 140 517 638 1562 1736 2018 2078 2988
Size: 140 377 121 924 174 282 60 910
Cuts at: 2988 3452 3998 4609 4621 4845
Size: 464 546 611 12 224
Fragments arranged by size:
924 910 611 546 464 377 282 224
174 140 121 60 12
BsaWI w'CCGG_w
Cuts at: 0 4022 4421 4845
Size: 4022 399 424
Fragments arranged by size:
4022 424 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1327 1357 1957 1987 3220 3250 4845
Size: 1327 30 600 30 1233 30 1595
Fragments arranged by size:
1595 1327 1233 600 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 628 913 1036 2679 3643 3889 4806
Size: 265 363 285 123 1643 964 246 917
Cuts at: 4806 4845
Size: 39
Fragments arranged by size:
1643 964 917 363 285 265 246 123
39
BseYI C'CCAG_C
Cuts at: 0 1659 2499 2806 3023 4328 4845
Size: 1659 840 307 217 1305 517
Fragments arranged by size:
1659 1305 840 517 307 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1000 1381 1854 2578 3484 4815 4845
Size: 316 684 381 473 724 906 1331 30
Fragments arranged by size:
1331 906 724 684 473 381 316 30
BsiEI CG_ry'CG
Cuts at: 0 197 1268 2628 3513 4422 4845
Size: 197 1071 1360 885 909 423
Fragments arranged by size:
1360 1071 909 885 423 197
BsiHKAI G_wGCw'C
Cuts at: 0 434 1715 2122 2132 3715 3737 3895 4103
Size: 434 1281 407 10 1583 22 158 208
Cuts at: 4103 4378 4845
Size: 275 467
Fragments arranged by size:
1583 1281 467 434 407 275 208 158
22 10
BsiWI C'GTAC_G
Cuts at: 0 126 4845
Size: 126 4719
BsmBI CGTCTCn'nnnn_
Cuts at: 0 508 1553 4845
Size: 508 1045 3292
Fragments arranged by size:
3292 1045 508
Bsp1286I G_dGCh'C
Cuts at: 0 434 831 1309 1382 1715 1772 2122 2132
Size: 434 397 478 73 333 57 350 10
Cuts at: 2132 3654 3715 3737 3895 4103 4166 4262 4301
Size: 1522 61 22 158 208 63 96 39
Cuts at: 4301 4378 4412 4845
Size: 77 34 433
Fragments arranged by size:
1522 478 434 433 397 350 333 208
158 96 77 73 63 61 57 39
34 22 10
BspHI T'CATG_A
Cuts at: 0 2108 4845
Size: 2108 2737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1092 1541 1924 3755 3781 3944 4845
Size: 1092 449 383 1831 26 163 901
Fragments arranged by size:
1831 1092 901 449 383 163 26
BsrBI CCG'CTC
Cuts at: 0 99 481 531 773 870 1479 1707 2010
Size: 99 382 50 242 97 609 228 303
Cuts at: 2010 2511 2607 3161 3977 4034 4845
Size: 501 96 554 816 57 811
Fragments arranged by size:
816 811 609 554 501 382 303 242
228 99 97 96 57 50
BsrDI GCAATG_nn'
Cuts at: 0 1124 4845
Size: 1124 3721
BsrFI r'CCGG_y
Cuts at: 0 142 3325 4845
Size: 142 3183 1520
Fragments arranged by size:
3183 1520 142
BsrGI T'GTAC_A
Cuts at: 0 4529 4845
Size: 4529 316
BssHII G'CGCG_C
Cuts at: 0 3914 4845
Size: 3914 931
BssSI C'ACGA_G
Cuts at: 0 2263 3730 4270 4845
Size: 2263 1467 540 575
Fragments arranged by size:
2263 1467 575 540
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3747 4355 4845
Size: 3747 608 490
Fragments arranged by size:
3747 608 490
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 471 609 1521 1890 2499 3177 3595
Size: 171 300 138 912 369 609 678 418
Cuts at: 3595 4845
Size: 1250
Fragments arranged by size:
1250 912 678 609 418 369 300 171
138
BstYI r'GATC_y
Cuts at: 0 818 1061 1352 1970 2304 2901 4845
Size: 818 243 291 618 334 597 1944
Fragments arranged by size:
1944 818 618 597 334 291 243
Bsu36I CC'TnA_GG
Cuts at: 0 1928 4845
Size: 1928 2917
BtgI C'CryG_G
Cuts at: 0 25 86 842 971 3170 3706 4514 4845
Size: 25 61 756 129 2199 536 808 331
Fragments arranged by size:
2199 808 756 536 331 129 61 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 450 1245 1374 3872 4335 4845
Size: 450 795 129 2498 463 510
Fragments arranged by size:
2498 795 510 463 450 129
BtsI GCAGTG_nn'
Cuts at: 0 206 1393 2740 3614 4066 4158 4568 4845
Size: 206 1187 1347 874 452 92 410 277
Fragments arranged by size:
1347 1187 874 452 410 277 206 92
DraIII CAC_nnn'GTG
Cuts at: 0 1404 2136 3732 4845
Size: 1404 732 1596 1113
Fragments arranged by size:
1596 1404 1113 732
EaeI y'GGCC_r
Cuts at: 0 28 194 401 1787 2000 2168 2625 2766
Size: 28 166 207 1386 213 168 457 141
Cuts at: 2766 4035 4511 4565 4845
Size: 1269 476 54 280
Fragments arranged by size:
1386 1269 476 457 280 213 207 168
166 141 54 28
EagI C'GGCC_G
Cuts at: 0 194 2625 4845
Size: 194 2431 2220
Fragments arranged by size:
2431 2220 194
EarI CTCTTCn'nnn_
Cuts at: 0 439 802 1600 3322 4195 4557 4813 4845
Size: 439 363 798 1722 873 362 256 32
Fragments arranged by size:
1722 873 798 439 363 362 256 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 161 1059 1626 3325 3885 4003 4771 4845
Size: 161 898 567 1699 560 118 768 74
Fragments arranged by size:
1699 898 768 567 560 161 118 74
EcoICRI GAG'CTC
Cuts at: 0 2120 4101 4376 4845
Size: 2120 1981 275 469
Fragments arranged by size:
2120 1981 469 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 205 631 1642 1912 2107 3418 3466
Size: 178 27 426 1011 270 195 1311 48
Cuts at: 3466 3646 4845
Size: 180 1199
Fragments arranged by size:
1311 1199 1011 426 270 195 180 178
48 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1537 1621 2644 4845
Size: 1537 84 1023 2201
Fragments arranged by size:
2201 1537 1023 84
EcoO109I rG'GnC_Cy
Cuts at: 0 444 1368 3020 3312 3827 4259 4286 4439
Size: 444 924 1652 292 515 432 27 153
Cuts at: 4439 4586 4845
Size: 147 259
Fragments arranged by size:
1652 924 515 444 432 292 259 153
147 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 450 666 965 4845
Size: 450 216 299 3880
Fragments arranged by size:
3880 450 299 216
HaeII r_GCGC'y
Cuts at: 0 13 64 1695 2451 2991 3837 4242 4612
Size: 13 51 1631 756 540 846 405 370
Cuts at: 4612 4661 4845
Size: 49 184
Fragments arranged by size:
1631 846 756 540 405 370 184 51
49 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 587 619 1946 1978 4684 4716 4845
Size: 587 32 1327 32 2706 32 129
Fragments arranged by size:
2706 1327 587 129 32 32 32
HincII GTy'rAC
Cuts at: 0 1233 1269 2175 3222 4845
Size: 1233 36 906 1047 1623
Fragments arranged by size:
1623 1233 1047 906 36
KasI G'GCGC_C
Cuts at: 0 2987 4608 4845
Size: 2987 1621 237
Fragments arranged by size:
2987 1621 237
KpnI G_GTAC'C
Cuts at: 0 3240 4845
Size: 3240 1605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1343 1779 2078 2516 2851 4118 4845
Size: 1343 436 299 438 335 1267 727
Fragments arranged by size:
1343 1267 727 438 436 335 299
MscI TGG'CCA
Cuts at: 0 403 1789 2002 4845
Size: 403 1386 213 2843
Fragments arranged by size:
2843 1386 403 213
MslI CAynn'nnrTG
Cuts at: 0 169 397 853 976 1321 2476 2677 3175
Size: 169 228 456 123 345 1155 201 498
Cuts at: 3175 3253 3647 3708 4151 4274 4540 4636 4845
Size: 78 394 61 443 123 266 96 209
Fragments arranged by size:
1155 498 456 443 394 345 266 228
209 201 169 123 123 96 78 61
MspA1I CmG'CkG
Cuts at: 0 33 88 179 984 1752 2745 2774 4283
Size: 33 55 91 805 768 993 29 1509
Cuts at: 4283 4667 4766 4845
Size: 384 99 79
Fragments arranged by size:
1509 993 805 768 384 99 91 79
55 33 29
NaeI GCC'GGC
Cuts at: 0 144 4845
Size: 144 4701
NarI GG'CG_CC
Cuts at: 0 2988 4609 4845
Size: 2988 1621 236
Fragments arranged by size:
2988 1621 236
NcoI C'CATG_G
Cuts at: 0 842 3170 4845
Size: 842 2328 1675
Fragments arranged by size:
2328 1675 842
NgoMIV G'CCGG_C
Cuts at: 0 142 4845
Size: 142 4703
NotI GC'GGCC_GC
Cuts at: 0 194 4845
Size: 194 4651
NspI r_CATG'y
Cuts at: 0 495 962 2499 3258 3797 4358 4545 4845
Size: 495 467 1537 759 539 561 187 300
Fragments arranged by size:
1537 759 561 539 495 467 300 187
PasI CC'CwG_GG
Cuts at: 0 3206 4845
Size: 3206 1639
PciI A'CATG_T
Cuts at: 0 491 4541 4845
Size: 491 4050 304
Fragments arranged by size:
4050 491 304
PflMI CCAn_nnn'nTGG
Cuts at: 0 3609 4227 4667 4845
Size: 3609 618 440 178
Fragments arranged by size:
3609 618 440 178
PfoI T'CCnGG_A
Cuts at: 0 421 1619 2879 4845
Size: 421 1198 1260 1966
Fragments arranged by size:
1966 1260 1198 421
PmlI CAC'GTG
Cuts at: 0 2133 4296 4845
Size: 2133 2163 549
Fragments arranged by size:
2163 2133 549
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2104 2136 4690 4722 4845
Size: 2104 32 2554 32 123
Fragments arranged by size:
2554 2104 123 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 444 1368 3020 3312 4286 4845
Size: 444 924 1652 292 974 559
Fragments arranged by size:
1652 974 924 559 444 292
PshAI GACnn'nnGTC
Cuts at: 0 1228 2077 4845
Size: 1228 849 2768
Fragments arranged by size:
2768 1228 849
PspOMI G'GGCC_C
Cuts at: 0 4162 4258 4845
Size: 4162 96 587
Fragments arranged by size:
4162 587 96
PstI C_TGCA'G
Cuts at: 0 989 1103 1538 2824 3743 4630 4845
Size: 989 114 435 1286 919 887 215
Fragments arranged by size:
1286 989 919 887 435 215 114
PvuII CAG'CTG
Cuts at: 0 984 1752 2745 4766 4845
Size: 984 768 993 2021 79
Fragments arranged by size:
2021 993 984 768 79
RsrII CG'GwC_CG
Cuts at: 0 19 1311 4845
Size: 19 1292 3534
Fragments arranged by size:
3534 1292 19
SacI G_AGCT'C
Cuts at: 0 2122 4103 4378 4845
Size: 2122 1981 275 467
Fragments arranged by size:
2122 1981 467 275
SacII CC_GC'GG
Cuts at: 0 89 4845
Size: 89 4756
SalI G'TCGA_C
Cuts at: 0 1267 4845
Size: 1267 3578
SanDI GG'GwC_CC
Cuts at: 0 3020 4286 4845
Size: 3020 1266 559
Fragments arranged by size:
3020 1266 559
SapI GCTCTTCn'nnn_
Cuts at: 0 439 4845
Size: 439 4406
SbfI CC_TGCA'GG
Cuts at: 0 1538 4845
Size: 1538 3307
ScaI AGT'ACT
Cuts at: 0 4684 4845
Size: 4684 161
SfcI C'TryA_G
Cuts at: 0 985 1099 1534 2820 3099 3739 4626 4653
Size: 985 114 435 1286 279 640 887 27
Cuts at: 4653 4845
Size: 192
Fragments arranged by size:
1286 985 887 640 435 279 192 114
27
SfoI GGC'GCC
Cuts at: 0 2989 4610 4845
Size: 2989 1621 235
Fragments arranged by size:
2989 1621 235
SgrAI Cr'CCGG_yG
Cuts at: 0 142 4845
Size: 142 4703
SmaI CCC'GGG
Cuts at: 0 3825 4319 4845
Size: 3825 494 526
Fragments arranged by size:
3825 526 494
SmlI C'TyrA_G
Cuts at: 0 1978 4845
Size: 1978 2867
SphI G_CATG'C
Cuts at: 0 4358 4845
Size: 4358 487
StuI AGG'CCT
Cuts at: 0 1933 2029 4845
Size: 1933 96 2816
Fragments arranged by size:
2816 1933 96
StyI C'CwwG_G
Cuts at: 0 218 470 842 1790 2312 3008 3051 3170
Size: 218 252 372 948 522 696 43 119
Cuts at: 3170 3486 3689 4845
Size: 316 203 1156
Fragments arranged by size:
1156 948 696 522 372 316 252 218
203 119 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 855 1263 1312 2347 2476 2536 2967
Size: 339 516 408 49 1035 129 60 431
Cuts at: 2967 3372 3659 4189 4743 4845
Size: 405 287 530 554 102
Fragments arranged by size:
1035 554 530 516 431 408 405 339
287 129 102 60 49
TatI w'GTAC_w
Cuts at: 0 230 365 494 749 1418 2558 3242 4529
Size: 230 135 129 255 669 1140 684 1287
Cuts at: 4529 4682 4845
Size: 153 163
Fragments arranged by size:
1287 1140 684 669 255 230 163 153
135 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1957 1989 4845
Size: 1957 32 2856
Fragments arranged by size:
2856 1957 32
XhoI C'TCGA_G
Cuts at: 0 1978 4845
Size: 1978 2867
XmaI C'CCGG_G
Cuts at: 0 3823 4317 4845
Size: 3823 494 528
Fragments arranged by size:
3823 528 494
ZraI GAC'GTC
Cuts at: 0 1737 2079 4845
Size: 1737 342 2766
Fragments arranged by size:
2766 1737 342
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgrAI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XhoI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI ApoI AscI AseI AsiSI
AvrII BaeI BfrBI BglII BmtI BpuEI BsmI BspEI
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI
NdeI NheI NruI NsiI PacI PmeI PsiI PspXI
PsrI PvuI SexAI SfiI SgfI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XcmI XmnI