Restriction map for
pFN21AB3246
(Linear) (Six-base) MAPSORT of: KIBB3246.seq Check: 528 from: 1 to: 3126
KIBB3246.orf
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 958 3126
Size: 958 2168
AatII G_ACGT'C
Cuts at: 0 1945 3126
Size: 1945 1181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 730 944 1571 2053 2149 3126
Size: 149 581 214 627 482 96 977
Fragments arranged by size:
977 627 581 482 214 149 96
AflII C'TTAA_G
Cuts at: 0 94 355 3126
Size: 94 261 2771
Fragments arranged by size:
2771 261 94
AhdI GACnn_n'nnGTC
Cuts at: 0 1473 1941 3126
Size: 1473 468 1185
Fragments arranged by size:
1473 1185 468
AleI CACnn'nnGTG
Cuts at: 0 1891 2170 2224 3126
Size: 1891 279 54 902
Fragments arranged by size:
1891 902 279 54
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1425 3126
Size: 1425 1701
AlwNI CAG_nnn'CTG
Cuts at: 0 1327 2898 3126
Size: 1327 1571 228
Fragments arranged by size:
1571 1327 228
ApaLI G'TGCA_C
Cuts at: 0 1569 3126
Size: 1569 1557
ApoI r'AATT_y
Cuts at: 0 763 1444 2062 3126
Size: 763 681 618 1064
Fragments arranged by size:
1064 763 681 618
AvaI C'yCGr_G
Cuts at: 0 2027 2347 3126
Size: 2027 320 779
Fragments arranged by size:
2027 779 320
BamHI G'GATC_C
Cuts at: 0 1797 3126
Size: 1797 1329
BanII G_rGCy'C
Cuts at: 0 112 2161 2803 3126
Size: 112 2049 642 323
Fragments arranged by size:
2049 642 323 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 3126
Size: 179 2947
BbvCI CC'TCA_GC
Cuts at: 0 2764 3126
Size: 2764 362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1985 2019 2104 2138 3126
Size: 1985 34 85 34 988
Fragments arranged by size:
1985 988 85 34 34
BclI T'GATC_A
Cuts at: 0 1865 2480 3126
Size: 1865 615 646
Fragments arranged by size:
1865 646 615
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1913 3126
Size: 47 1866 1213
Fragments arranged by size:
1866 1213 47
BlpI GC'TnA_GC
Cuts at: 0 70 3126
Size: 70 3056
Bme1580I G_kGCm'C
Cuts at: 0 833 1573 1888 3126
Size: 833 740 315 1238
Fragments arranged by size:
1238 833 740 315
BmgBI CAC'GTC
Cuts at: 0 1938 3126
Size: 1938 1188
BmrI ACTGGGnnnn_n'
Cuts at: 0 1880 2425 2886 3126
Size: 1880 545 461 240
Fragments arranged by size:
1880 545 461 240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1836 3126
Size: 1836 1290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1634 3126
Size: 698 936 1492
Fragments arranged by size:
1492 936 698
Bpu10I CC'TnA_GC
Cuts at: 0 2764 3126
Size: 2764 362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1750 2932 3126
Size: 1750 1182 194
Fragments arranged by size:
1750 1182 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 1753 3126
Size: 1753 1373
BsaAI yAC'GTr
Cuts at: 0 1465 3126
Size: 1465 1661
BsaHI Gr'CG_yC
Cuts at: 0 50 1942 2000 3126
Size: 50 1892 58 1126
Fragments arranged by size:
1892 1126 58 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 653 683 2200 2230 3126
Size: 653 30 1517 30 896
Fragments arranged by size:
1517 896 653 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 1812 2254 3126
Size: 43 1769 442 872
Fragments arranged by size:
1769 872 442 43
BseYI C'CCAG_C
Cuts at: 0 36 646 1991 3126
Size: 36 610 1345 1135
Fragments arranged by size:
1345 1135 610 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 2396 3013 3126
Size: 430 1966 617 113
Fragments arranged by size:
1966 617 430 113
BsiHKAI G_wGCw'C
Cuts at: 0 1254 1548 1573 3126
Size: 1254 294 25 1553
Fragments arranged by size:
1553 1254 294 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1991 2935 3126
Size: 1991 944 191
Fragments arranged by size:
1991 944 191
Bsp1286I G_dGCh'C
Cuts at: 0 112 833 1254 1548 1573 1888 2161 2803
Size: 112 721 421 294 25 315 273 642
Cuts at: 2803 3126
Size: 323
Fragments arranged by size:
721 642 421 323 315 294 273 112
25
BspHI T'CATG_A
Cuts at: 0 1695 1862 2444 3126
Size: 1695 167 582 682
Fragments arranged by size:
1695 682 582 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 958 3126
Size: 958 2168
BsrDI GCAATG_nn'
Cuts at: 0 638 803 2147 2564 3126
Size: 638 165 1344 417 562
Fragments arranged by size:
1344 638 562 417 165
BsrFI r'CCGG_y
Cuts at: 0 123 1838 3126
Size: 123 1715 1288
Fragments arranged by size:
1715 1288 123
BsrGI T'GTAC_A
Cuts at: 0 1492 3126
Size: 1492 1634
BssSI C'ACGA_G
Cuts at: 0 590 2794 3126
Size: 590 2204 332
Fragments arranged by size:
2204 590 332
BstBI TT'CG_AA
Cuts at: 0 999 3126
Size: 999 2127
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 954 1222 2226 2415 3126
Size: 24 930 268 1004 189 711
Fragments arranged by size:
1004 930 711 268 189 24
BstYI r'GATC_y
Cuts at: 0 701 1797 3126
Size: 701 1096 1329
Fragments arranged by size:
1329 1096 701
BtgI C'CryG_G
Cuts at: 0 2540 3126
Size: 2540 586
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1689 2117 3126
Size: 1689 428 1009
Fragments arranged by size:
1689 1009 428
BtsI GCAGTG_nn'
Cuts at: 0 902 1669 1886 2165 2408 3126
Size: 902 767 217 279 243 718
Fragments arranged by size:
902 767 718 279 243 217
DraI TTT'AAA
Cuts at: 0 888 3126
Size: 888 2238
EaeI y'GGCC_r
Cuts at: 0 1688 1895 3046 3126
Size: 1688 207 1151 80
Fragments arranged by size:
1688 1151 207 80
EarI CTCTTCn'nnn_
Cuts at: 0 1707 3126
Size: 1707 1419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1977 2218 3126
Size: 1977 241 908
Fragments arranged by size:
1977 908 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 698 730 944 1571 1634 2053 2149
Size: 149 549 32 214 627 63 419 96
Cuts at: 2149 3126
Size: 977
Fragments arranged by size:
977 627 549 419 214 149 96 63
32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 2763 3126
Size: 79 2684 363
Fragments arranged by size:
2684 363 79
EcoO109I rG'GnC_Cy
Cuts at: 0 857 3126
Size: 857 2269
EcoRI G'AATT_C
Cuts at: 0 1444 3126
Size: 1444 1682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 512 3126
Size: 512 2614
HaeII r_GCGC'y
Cuts at: 0 2534 2698 3126
Size: 2534 164 428
Fragments arranged by size:
2534 428 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1459 1491 1927 1959 2200 2232 3126
Size: 1459 32 436 32 241 32 894
Fragments arranged by size:
1459 894 436 241 32 32 32
HincII GTy'rAC
Cuts at: 0 54 494 525 600 684 3126
Size: 54 440 31 75 84 2442
Fragments arranged by size:
2442 440 84 75 54 31
HindIII A'AGCT_T
Cuts at: 0 91 1949 3126
Size: 91 1858 1177
Fragments arranged by size:
1858 1177 91
HpaI GTT'AAC
Cuts at: 0 494 684 3126
Size: 494 190 2442
Fragments arranged by size:
2442 494 190
MfeI C'AATT_G
Cuts at: 0 305 3126
Size: 305 2821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1290 1566 2321 3126
Size: 137 1153 276 755 805
Fragments arranged by size:
1153 805 755 276 137
MscI TGG'CCA
Cuts at: 0 1690 1897 3126
Size: 1690 207 1229
Fragments arranged by size:
1690 1229 207
MslI CAynn'nnrTG
Cuts at: 0 836 1190 1217 1674 1891 2152 2170 2224
Size: 836 354 27 457 217 261 18 54
Cuts at: 2224 3126
Size: 902
Fragments arranged by size:
902 836 457 354 261 217 54 27
18
MspA1I CmG'CkG
Cuts at: 0 414 1431 2018 2370 2486 2604 2880 3126
Size: 414 1017 587 352 116 118 276 246
Fragments arranged by size:
1017 587 414 352 276 246 118 116
NaeI GCC'GGC
Cuts at: 0 1840 3126
Size: 1840 1286
NgoMIV G'CCGG_C
Cuts at: 0 1838 3126
Size: 1838 1288
NspI r_CATG'y
Cuts at: 0 209 621 1794 3029 3126
Size: 209 412 1173 1235 97
Fragments arranged by size:
1235 1173 412 209 97
PasI CC'CwG_GG
Cuts at: 0 2777 3126
Size: 2777 349
PfoI T'CCnGG_A
Cuts at: 0 704 859 3126
Size: 704 155 2267
Fragments arranged by size:
2267 704 155
PmlI CAC'GTG
Cuts at: 0 1465 3126
Size: 1465 1661
PpuMI rG'GwC_Cy
Cuts at: 0 857 3126
Size: 857 2269
PshAI GACnn'nnGTC
Cuts at: 0 2579 3126
Size: 2579 547
PspXI vC'TCGA_Gb
Cuts at: 0 2027 3126
Size: 2027 1099
PstI C_TGCA'G
Cuts at: 0 939 1093 3126
Size: 939 154 2033
Fragments arranged by size:
2033 939 154
PvuII CAG'CTG
Cuts at: 0 1431 2370 2604 3126
Size: 1431 939 234 522
Fragments arranged by size:
1431 939 522 234
ScaI AGT'ACT
Cuts at: 0 1593 3126
Size: 1593 1533
SexAI A'CCwGG_T
Cuts at: 0 2976 3126
Size: 2976 150
SfcI C'TryA_G
Cuts at: 0 908 935 1089 1319 2617 2714 3087 3126
Size: 908 27 154 230 1298 97 373 39
Fragments arranged by size:
1298 908 373 230 154 97 39 27
SmlI C'TyrA_G
Cuts at: 0 94 355 1729 2027 2947 3126
Size: 94 261 1374 298 920 179
Fragments arranged by size:
1374 920 298 261 179 94
SphI G_CATG'C
Cuts at: 0 1794 3029 3126
Size: 1794 1235 97
Fragments arranged by size:
1794 1235 97
SspI AAT'ATT
Cuts at: 0 825 3126
Size: 825 2301
StyI C'CwwG_G
Cuts at: 0 1023 2337 3126
Size: 1023 1314 789
Fragments arranged by size:
1314 1023 789
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 1588 2533 3126
Size: 301 1287 945 593
Fragments arranged by size:
1287 945 593 301
TatI w'GTAC_w
Cuts at: 0 1030 1492 1591 3126
Size: 1030 462 99 1535
Fragments arranged by size:
1535 1030 462 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 855 887 2813 2845 2849 2881 3126
Size: 855 32 1926 32 4 32 245
Fragments arranged by size:
1926 855 245 32 32 32 4
XbaI T'CTAG_A
Cuts at: 0 1452 3126
Size: 1452 1674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 1582 3126
Size: 306 1276 1544
Fragments arranged by size:
1544 1276 306
XhoI C'TCGA_G
Cuts at: 0 2027 3126
Size: 2027 1099
XmnI GAAnn'nnTTC
Cuts at: 0 2066 2272 3126
Size: 2066 206 854
Fragments arranged by size:
2066 854 206
ZraI GAC'GTC
Cuts at: 0 1943 3126
Size: 1943 1183
Enzymes that do cut:
AarI AatII AcuI AflII AhdI AleI AlfI AlwNI
ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PasI PfoI
PmlI PpuMI PshAI PspXI PstI PvuII ScaI SexAI
SfcI SmlI SphI SspI StyI TaqII TatI TstI
XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflIII AgeI AloI ApaI
AscI AseI AsiSI AvrII BaeI BanI BbeI BciVI
BfrBI BglII BmtI BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BsrBI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI
CspCI DraIII DrdI EagI EcoICRI EcoRV FseI FspI
FspAI KasI KpnI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PpiI
PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI Tth111I XmaI