Restriction map for
pFN21AB3164
(Linear) (Six-base) MAPSORT of: KIBB3164.seq Check: 1128 from: 1 to: 2763
KIBB3164.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 0 605 2763
Size: 605 2158
AccI GT'mk_AC
Cuts at: 0 599 2763
Size: 599 2164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 995 1306 2014 2530 2584 2763
Size: 51 944 311 708 516 54 179
Fragments arranged by size:
944 708 516 311 179 54 51
AflII C'TTAA_G
Cuts at: 0 826 2763
Size: 826 1937
AflIII A'CryG_T
Cuts at: 0 534 2763
Size: 534 2229
AgeI A'CCGG_T
Cuts at: 0 2279 2763
Size: 2279 484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 787 2763
Size: 128 659 1976
Fragments arranged by size:
1976 659 128
AlwNI CAG_nnn'CTG
Cuts at: 0 194 375 1050 2475 2508 2607 2763
Size: 194 181 675 1425 33 99 156
Fragments arranged by size:
1425 675 194 181 156 99 33
ApaLI G'TGCA_C
Cuts at: 0 2724 2763
Size: 2724 39
ApoI r'AATT_y
Cuts at: 0 274 889 946 1244 1604 2763
Size: 274 615 57 298 360 1159
Fragments arranged by size:
1159 615 360 298 274 57
BamHI G'GATC_C
Cuts at: 0 777 966 2763
Size: 777 189 1797
Fragments arranged by size:
1797 777 189
BanI G'GyrC_C
Cuts at: 0 465 558 2763
Size: 465 93 2205
Fragments arranged by size:
2205 465 93
BanII G_rGCy'C
Cuts at: 0 2720 2763
Size: 2720 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 1004 1172 1715 2071 2247 2763
Size: 1004 168 543 356 176 516
Fragments arranged by size:
1004 543 516 356 176 168
BbvCI CC'TCA_GC
Cuts at: 0 883 2763
Size: 883 1880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2030 2064 2763
Size: 2030 34 699
Fragments arranged by size:
2030 699 34
BclI T'GATC_A
Cuts at: 0 1956 2763
Size: 1956 807
BfrBI ATG'CAT
Cuts at: 0 1557 2763
Size: 1557 1206
BglII A'GATC_T
Cuts at: 0 171 291 1152 1841 2154 2763
Size: 171 120 861 689 313 609
Fragments arranged by size:
861 689 609 313 171 120
Bme1580I G_kGCm'C
Cuts at: 0 2728 2763
Size: 2728 35
BmgBI CAC'GTC
Cuts at: 0 15 535 2493 2763
Size: 15 520 1958 270
Fragments arranged by size:
1958 520 270 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 110 2763
Size: 110 2653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1400 2464 2558 2722 2763
Size: 548 852 1064 94 164 41
Fragments arranged by size:
1064 852 548 164 94 41
Bpu10I CC'TnA_GC
Cuts at: 0 883 1666 2763
Size: 883 783 1097
Fragments arranged by size:
1097 883 783
BsaI GGTCTCn'nnnn_
Cuts at: 0 2014 2440 2763
Size: 2014 426 323
Fragments arranged by size:
2014 426 323
BsaBI GATnn'nnATC
Cuts at: 0 1133 2763
Size: 1133 1630
BsaHI Gr'CG_yC
Cuts at: 0 602 2763
Size: 602 2161
BsaWI w'CCGG_w
Cuts at: 0 2279 2453 2763
Size: 2279 174 310
Fragments arranged by size:
2279 310 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 521 551 991 1021 1368 1398 2298 2328
Size: 521 30 440 30 347 30 900 30
Cuts at: 2328 2556 2586 2763
Size: 228 30 177
Fragments arranged by size:
900 521 440 347 228 177 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 999 1373 2763
Size: 999 374 1390
Fragments arranged by size:
1390 999 374
BseYI C'CCAG_C
Cuts at: 0 1767 2763
Size: 1767 996
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 2543 2763
Size: 938 1605 220
Fragments arranged by size:
1605 938 220
BsiHKAI G_wGCw'C
Cuts at: 0 1857 2720 2728 2763
Size: 1857 863 8 35
Fragments arranged by size:
1857 863 35 8
BsmI GAATG_Cn'
Cuts at: 0 260 1910 2191 2763
Size: 260 1650 281 572
Fragments arranged by size:
1650 572 281 260
Bsp1286I G_dGCh'C
Cuts at: 0 1857 2720 2728 2763
Size: 1857 863 8 35
Fragments arranged by size:
1857 863 35 8
BspEI T'CCGG_A
Cuts at: 0 2453 2763
Size: 2453 310
BspHI T'CATG_A
Cuts at: 0 2361 2763
Size: 2361 402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1996 2763
Size: 1996 767
BsrBI CCG'CTC
Cuts at: 0 834 2763
Size: 834 1929
BsrDI GCAATG_nn'
Cuts at: 0 2051 2552 2763
Size: 2051 501 211
Fragments arranged by size:
2051 501 211
BsrFI r'CCGG_y
Cuts at: 0 2279 2763
Size: 2279 484
BsrGI T'GTAC_A
Cuts at: 0 335 2763
Size: 335 2428
BssSI C'ACGA_G
Cuts at: 0 2416 2464 2719 2763
Size: 2416 48 255 44
Fragments arranged by size:
2416 255 48 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1662 2533 2763
Size: 1662 871 230
Fragments arranged by size:
1662 871 230
BstBI TT'CG_AA
Cuts at: 0 272 2763
Size: 272 2491
BstXI CCAn_nnnn'nTGG
Cuts at: 0 722 2763
Size: 722 2041
BstYI r'GATC_y
Cuts at: 0 171 291 777 966 1152 1841 2154 2459
Size: 171 120 486 189 186 689 313 305
Cuts at: 2459 2763
Size: 304
Fragments arranged by size:
689 486 313 305 304 189 186 171
120
Bsu36I CC'TnA_GG
Cuts at: 0 1011 2501 2763
Size: 1011 1490 262
Fragments arranged by size:
1490 1011 262
BtgI C'CryG_G
Cuts at: 0 820 2057 2763
Size: 820 1237 706
Fragments arranged by size:
1237 820 706
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1223 2440 2763
Size: 1223 1217 323
Fragments arranged by size:
1223 1217 323
BtsI GCAGTG_nn'
Cuts at: 0 727 1095 2763
Size: 727 368 1668
Fragments arranged by size:
1668 727 368
DraI TTT'AAA
Cuts at: 0 1403 1514 2582 2763
Size: 1403 111 1068 181
Fragments arranged by size:
1403 1068 181 111
DraIII CAC_nnn'GTG
Cuts at: 0 2230 2763
Size: 2230 533
EaeI y'GGCC_r
Cuts at: 0 2 2763
Size: 2 2761
EarI CTCTTCn'nnn_
Cuts at: 0 971 1058 2603 2763
Size: 971 87 1545 160
Fragments arranged by size:
1545 971 160 87
EcoICRI GAG'CTC
Cuts at: 0 2718 2763
Size: 2718 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 548 995 1306 1400 2014 2464 2530
Size: 51 497 447 311 94 614 450 66
Cuts at: 2530 2558 2584 2722 2763
Size: 28 26 138 41
Fragments arranged by size:
614 497 450 447 311 138 94 66
51 41 28 26
EcoO109I rG'GnC_Cy
Cuts at: 0 61 1098 2514 2742 2763
Size: 61 1037 1416 228 21
Fragments arranged by size:
1416 1037 228 61 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 599 1603 2763
Size: 81 518 1004 1160
Fragments arranged by size:
1160 1004 518 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 762 794 991 1023 1798 1826
Size: 71 32 659 32 197 32 775 28
Cuts at: 1826 1830 1858 2578 2610 2763
Size: 4 28 720 32 153
Fragments arranged by size:
775 720 659 197 153 71 32 32
32 32 28 28 4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 2763
Size: 519 2244
MslI CAynn'nnrTG
Cuts at: 0 419 769 1441 1595 1627 2056 2420 2763
Size: 419 350 672 154 32 429 364 343
Fragments arranged by size:
672 429 419 364 350 343 154 32
MspA1I CmG'CkG
Cuts at: 0 784 882 931 2090 2763
Size: 784 98 49 1159 673
Fragments arranged by size:
1159 784 673 98 49
NcoI C'CATG_G
Cuts at: 0 820 2057 2763
Size: 820 1237 706
Fragments arranged by size:
1237 820 706
NdeI CA'TA_TG
Cuts at: 0 200 2166 2640 2763
Size: 200 1966 474 123
Fragments arranged by size:
1966 474 200 123
NsiI A_TGCA'T
Cuts at: 0 1559 2763
Size: 1559 1204
NspI r_CATG'y
Cuts at: 0 768 2763
Size: 768 1995
PflMI CCAn_nnn'nTGG
Cuts at: 0 1644 1774 2763
Size: 1644 130 989
Fragments arranged by size:
1644 989 130
PfoI T'CCnGG_A
Cuts at: 0 1124 2763
Size: 1124 1639
PpuMI rG'GwC_Cy
Cuts at: 0 61 1098 2514 2742 2763
Size: 61 1037 1416 228 21
Fragments arranged by size:
1416 1037 228 61 21
PstI C_TGCA'G
Cuts at: 0 1090 2007 2529 2612 2763
Size: 1090 917 522 83 151
Fragments arranged by size:
1090 917 522 151 83
PvuII CAG'CTG
Cuts at: 0 784 882 2090 2763
Size: 784 98 1208 673
Fragments arranged by size:
1208 784 673 98
SacI G_AGCT'C
Cuts at: 0 2720 2763
Size: 2720 43
SanDI GG'GwC_CC
Cuts at: 0 61 2742 2763
Size: 61 2681 21
Fragments arranged by size:
2681 61 21
ScaI AGT'ACT
Cuts at: 0 923 2398 2763
Size: 923 1475 365
Fragments arranged by size:
1475 923 365
SfcI C'TryA_G
Cuts at: 0 347 596 1086 2003 2525 2608 2763
Size: 347 249 490 917 522 83 155
Fragments arranged by size:
917 522 490 347 249 155 83
SmlI C'TyrA_G
Cuts at: 0 826 2763
Size: 826 1937
SpeI A'CTAG_T
Cuts at: 0 2208 2763
Size: 2208 555
SphI G_CATG'C
Cuts at: 0 768 2763
Size: 768 1995
SspI AAT'ATT
Cuts at: 0 2134 2763
Size: 2134 629
StyI C'CwwG_G
Cuts at: 0 665 715 820 1877 2057 2763
Size: 665 50 105 1057 180 706
Fragments arranged by size:
1057 706 665 180 105 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 833 2288 2763
Size: 833 1455 475
Fragments arranged by size:
1455 833 475
TatI w'GTAC_w
Cuts at: 0 163 335 921 1215 2092 2120 2396 2763
Size: 163 172 586 294 877 28 276 367
Fragments arranged by size:
877 586 367 294 276 172 163 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 135 167 1368 1400 2498 2530 2726 2758
Size: 135 32 1201 32 1098 32 196 32
Cuts at: 2758 2763
Size: 5
Fragments arranged by size:
1201 1098 196 135 32 32 32 32
5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1233 1464 2763
Size: 1233 231 1299
Fragments arranged by size:
1299 1233 231
XmnI GAAnn'nnTTC
Cuts at: 0 1067 2597 2763
Size: 1067 1530 166
Fragments arranged by size:
1530 1067 166
ZraI GAC'GTC
Cuts at: 0 603 2763
Size: 603 2160
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AgeI AlfI AlwNI
ApaLI ApoI BamHI BanI BanII BbsI BbvCI BcgI
BclI BfrBI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I FalI Hin4I MmeI MslI MspA1I NcoI
NdeI NsiI NspI PflMI PfoI PpuMI PstI PvuII
SacI SanDI ScaI SfcI SmlI SpeI SphI SspI
StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AhdI AleI AloI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BciVI
BglI BlpI BmtI BplI BpuEI BsaAI BsiEI BsiWI
BsmBI BssHII BstEII BstZ17I ClaI CspCI DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI
SwaI Tth111I XbaI XhoI XmaI