Restriction map for
pFN21AB3007
(Linear) (Six-base) MAPSORT of: KIBB3007.seq Check: 7969 from: 1 to: 3747
KIBB3007.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AccI GT'mk_AC
Cuts at: 0 140 1102 1776 3747
Size: 140 962 674 1971
Fragments arranged by size:
1971 962 674 140
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1616 1684 1863 2039 2177 3086 3747
Size: 1616 68 179 176 138 909 661
Fragments arranged by size:
1616 909 661 179 176 138 68
AflII C'TTAA_G
Cuts at: 0 289 1809 3747
Size: 289 1520 1938
Fragments arranged by size:
1938 1520 289
AflIII A'CryG_T
Cuts at: 0 798 1791 3747
Size: 798 993 1956
Fragments arranged by size:
1956 993 798
AleI CACnn'nnGTG
Cuts at: 0 1456 3747
Size: 1456 2291
AlwNI CAG_nnn'CTG
Cuts at: 0 2893 3747
Size: 2893 854
ApaI G_GGCC'C
Cuts at: 0 1257 3747
Size: 1257 2490
ApoI r'AATT_y
Cuts at: 0 463 543 869 1008 1019 1725 1973 2113
Size: 463 80 326 139 11 706 248 140
Cuts at: 2113 2191 2551 3086 3598 3747
Size: 78 360 535 512 149
Fragments arranged by size:
706 535 512 463 360 326 248 149
140 139 80 78 11
AseI AT'TA_AT
Cuts at: 0 1034 3747
Size: 1034 2713
AvaI C'yCGr_G
Cuts at: 0 144 3747
Size: 144 3603
AvrII C'CTAG_G
Cuts at: 0 2172 3747
Size: 2172 1575
BamHI G'GATC_C
Cuts at: 0 1797 2911 3747
Size: 1797 1114 836
Fragments arranged by size:
1797 1114 836
BanII G_rGCy'C
Cuts at: 0 26 153 789 1257 1443 3747
Size: 26 127 636 468 186 2304
Fragments arranged by size:
2304 636 468 186 127 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 1220 1343 3301 3747
Size: 1220 123 1958 446
Fragments arranged by size:
1958 1220 446 123
BciVI GTATCCnnnnn_n'
Cuts at: 0 377 3734 3747
Size: 377 3357 13
Fragments arranged by size:
3357 377 13
BfrBI ATG'CAT
Cuts at: 0 1363 2296 3565 3747
Size: 1363 933 1269 182
Fragments arranged by size:
1363 1269 933 182
BglII A'GATC_T
Cuts at: 0 2346 3078 3747
Size: 2346 732 669
Fragments arranged by size:
2346 732 669
Bme1580I G_kGCm'C
Cuts at: 0 1257 3390 3747
Size: 1257 2133 357
Fragments arranged by size:
2133 1257 357
BmrI ACTGGGnnnn_n'
Cuts at: 0 2385 2389 3747
Size: 2385 4 1358
Fragments arranged by size:
2385 1358 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 2662 3747
Size: 636 2026 1085
Fragments arranged by size:
2026 1085 636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 476 672 1472 2772 3404 3747
Size: 358 118 196 800 1300 632 343
Fragments arranged by size:
1300 800 632 358 343 196 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 52 3747
Size: 46 6 3695
Fragments arranged by size:
3695 46 6
BsaAI yAC'GTr
Cuts at: 0 1792 3747
Size: 1792 1955
BsaBI GATnn'nnATC
Cuts at: 0 2615 3747
Size: 2615 1132
BsaWI w'CCGG_w
Cuts at: 0 61 1665 3747
Size: 61 1604 2082
Fragments arranged by size:
2082 1604 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 748 778 1008 1038 3747
Size: 748 30 230 30 2709
Fragments arranged by size:
2709 748 230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 797 1298 2267 3472 3747
Size: 34 763 501 969 1205 275
Fragments arranged by size:
1205 969 763 501 275 34
BseYI C'CCAG_C
Cuts at: 0 1747 3747
Size: 1747 2000
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1610 3747
Size: 1610 2137
BsiHKAI G_wGCw'C
Cuts at: 0 116 789 867 1443 3747
Size: 116 673 78 576 2304
Fragments arranged by size:
2304 673 576 116 78
Bsp1286I G_dGCh'C
Cuts at: 0 26 116 153 789 867 1257 1443 3390
Size: 26 90 37 636 78 390 186 1947
Cuts at: 3390 3747
Size: 357
Fragments arranged by size:
1947 636 390 357 186 90 78 37
26
BspEI T'CCGG_A
Cuts at: 0 1665 3747
Size: 1665 2082
BspHI T'CATG_A
Cuts at: 0 1763 2766 3747
Size: 1763 1003 981
Fragments arranged by size:
1763 1003 981
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 153 767 1215 1858 3747
Size: 153 614 448 643 1889
Fragments arranged by size:
1889 643 614 448 153
BsrBI CCG'CTC
Cuts at: 0 1875 3747
Size: 1875 1872
BsrDI GCAATG_nn'
Cuts at: 0 1289 1373 2198 3296 3747
Size: 1289 84 825 1098 451
Fragments arranged by size:
1289 1098 825 451 84
BsrGI T'GTAC_A
Cuts at: 0 1953 3747
Size: 1953 1794
BstBI TT'CG_AA
Cuts at: 0 1977 3747
Size: 1977 1770
BstXI CCAn_nnnn'nTGG
Cuts at: 0 697 1755 3747
Size: 697 1058 1992
Fragments arranged by size:
1992 1058 697
BstYI r'GATC_y
Cuts at: 0 1608 1797 2346 2911 3078 3747
Size: 1608 189 549 565 167 669
Fragments arranged by size:
1608 669 565 549 189 167
Bsu36I CC'TnA_GG
Cuts at: 0 318 365 2000 3747
Size: 318 47 1635 1747
Fragments arranged by size:
1747 1635 318 47
BtgI C'CryG_G
Cuts at: 0 1590 2256 3747
Size: 1590 666 1491
Fragments arranged by size:
1590 1491 666
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 102 236 2624 3747
Size: 102 134 2388 1123
Fragments arranged by size:
2388 1123 134 102
BtsI GCAGTG_nn'
Cuts at: 0 1451 1463 1892 2386 3747
Size: 1451 12 429 494 1361
Fragments arranged by size:
1451 1361 494 429 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1347 1382 3747
Size: 1347 35 2365
Fragments arranged by size:
2365 1347 35
DraI TTT'AAA
Cuts at: 0 1962 3050 3747
Size: 1962 1088 697
Fragments arranged by size:
1962 1088 697
DraIII CAC_nnn'GTG
Cuts at: 0 429 3747
Size: 429 3318
DrdI GACnn_nn'nnGTC
Cuts at: 0 136 3747
Size: 136 3611
EaeI y'GGCC_r
Cuts at: 0 2 1003 3747
Size: 2 1001 2744
Fragments arranged by size:
2744 1001 2
EarI CTCTTCn'nnn_
Cuts at: 0 276 492 1431 1882 2138 3050 3747
Size: 276 216 939 451 256 912 697
Fragments arranged by size:
939 912 697 451 276 256 216
EcoICRI GAG'CTC
Cuts at: 0 787 1441 3747
Size: 787 654 2306
Fragments arranged by size:
2306 787 654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1616 1684 1863 2039 2177 2662 3086
Size: 636 980 68 179 176 138 485 424
Cuts at: 3086 3747
Size: 661
Fragments arranged by size:
980 661 636 485 424 179 176 138
68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1865 2171 3747
Size: 1865 306 1576
Fragments arranged by size:
1865 1576 306
EcoO109I rG'GnC_Cy
Cuts at: 0 414 1237 1253 1254 2890 3747
Size: 414 823 16 1 1636 857
Fragments arranged by size:
1636 857 823 414 16 1
EcoRI G'AATT_C
Cuts at: 0 2113 3747
Size: 2113 1634
EcoRV GAT'ATC
Cuts at: 0 198 3747
Size: 198 3549
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1840 1872 1874 1906 2311 2343 3747
Size: 1840 32 2 32 405 32 1404
Fragments arranged by size:
1840 1404 405 32 32 32 2
HincII GTy'rAC
Cuts at: 0 141 975 2232 2452 3747
Size: 141 834 1257 220 1295
Fragments arranged by size:
1295 1257 834 220 141
HindIII A'AGCT_T
Cuts at: 0 2159 3747
Size: 2159 1588
MfeI C'AATT_G
Cuts at: 0 2186 3184 3747
Size: 2186 998 563
Fragments arranged by size:
2186 998 563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1560 3747
Size: 1560 2187
MscI TGG'CCA
Cuts at: 0 4 1005 3747
Size: 4 1001 2742
Fragments arranged by size:
2742 1001 4
MslI CAynn'nnrTG
Cuts at: 0 962 1456 1792 3747
Size: 962 494 336 1955
Fragments arranged by size:
1955 962 494 336
MspA1I CmG'CkG
Cuts at: 0 10 715 779 1329 3747
Size: 10 705 64 550 2418
Fragments arranged by size:
2418 705 550 64 10
NcoI C'CATG_G
Cuts at: 0 2256 3747
Size: 2256 1491
NsiI A_TGCA'T
Cuts at: 0 1365 2298 3567 3747
Size: 1365 933 1269 180
Fragments arranged by size:
1365 1269 933 180
NspI r_CATG'y
Cuts at: 0 162 802 1367 2074 3565 3747
Size: 162 640 565 707 1491 182
Fragments arranged by size:
1491 707 640 565 182 162
PasI CC'CwG_GG
Cuts at: 0 1802 3747
Size: 1802 1945
PciI A'CATG_T
Cuts at: 0 798 3747
Size: 798 2949
PflMI CCAn_nnn'nTGG
Cuts at: 0 256 1012 3747
Size: 256 756 2735
Fragments arranged by size:
2735 756 256
PfoI T'CCnGG_A
Cuts at: 0 125 3747
Size: 125 3622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1598 1630 3747
Size: 1598 32 2117
Fragments arranged by size:
2117 1598 32
PpuMI rG'GwC_Cy
Cuts at: 0 414 1237 2890 3747
Size: 414 823 1653 857
Fragments arranged by size:
1653 857 823 414
PsiI TTA'TAA
Cuts at: 0 952 3125 3747
Size: 952 2173 622
Fragments arranged by size:
2173 952 622
PspOMI G'GGCC_C
Cuts at: 0 1253 3747
Size: 1253 2494
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2948 2980 3747
Size: 2948 32 767
Fragments arranged by size:
2948 767 32
PstI C_TGCA'G
Cuts at: 0 1458 1899 3747
Size: 1458 441 1848
Fragments arranged by size:
1848 1458 441
PvuII CAG'CTG
Cuts at: 0 715 1329 3747
Size: 715 614 2418
Fragments arranged by size:
2418 715 614
SacI G_AGCT'C
Cuts at: 0 789 1443 3747
Size: 789 654 2304
Fragments arranged by size:
2304 789 654
SalI G'TCGA_C
Cuts at: 0 139 3747
Size: 139 3608
SanDI GG'GwC_CC
Cuts at: 0 414 3747
Size: 414 3333
SapI GCTCTTCn'nnn_
Cuts at: 0 492 1431 1882 3747
Size: 492 939 451 1865
Fragments arranged by size:
1865 939 492 451
SexAI A'CCwGG_T
Cuts at: 0 1965 3747
Size: 1965 1782
SfcI C'TryA_G
Cuts at: 0 236 447 978 1103 1454 1895 2668 3747
Size: 236 211 531 125 351 441 773 1079
Fragments arranged by size:
1079 773 531 441 351 236 211 125
SmaI CCC'GGG
Cuts at: 0 146 3747
Size: 146 3601
SmlI C'TyrA_G
Cuts at: 0 289 373 491 651 1487 1809 2751 3419
Size: 289 84 118 160 836 322 942 668
Cuts at: 3419 3747
Size: 328
Fragments arranged by size:
942 836 668 328 322 289 160 118
84
SphI G_CATG'C
Cuts at: 0 162 3747
Size: 162 3585
StuI AGG'CCT
Cuts at: 0 926 3309 3593 3747
Size: 926 2383 284 154
Fragments arranged by size:
2383 926 284 154
StyI C'CwwG_G
Cuts at: 0 1257 2172 2256 3747
Size: 1257 915 84 1491
Fragments arranged by size:
1491 1257 915 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2841 3427 3747
Size: 2841 586 320
Fragments arranged by size:
2841 586 320
TatI w'GTAC_w
Cuts at: 0 1953 2269 3747
Size: 1953 316 1478
Fragments arranged by size:
1953 1478 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1008 1040 3369 3401 3672 3704 3747
Size: 1008 32 2329 32 271 32 43
Fragments arranged by size:
2329 1008 271 43 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1231 1443 3747
Size: 1231 212 2304
Fragments arranged by size:
2304 1231 212
XmaI C'CCGG_G
Cuts at: 0 144 3747
Size: 144 3603
XmnI GAAnn'nnTTC
Cuts at: 0 2162 3747
Size: 2162 1585
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaI ApoI
AseI AvaI AvrII BamHI BanII BbsI BciVI BfrBI
BglII Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrGI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NcoI NsiI NspI PasI PciI PflMI PfoI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuII SacI SalI
SanDI SapI SexAI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaLI AscI AsiSI BaeI BanI BbeI BbvCI
BcgI BclI BglI BlpI BmgBI BmtI BplI Bpu10I
BsaHI BsiEI BsiWI BsmI BsmBI BsrFI BssHII BssSI
BstAPI BstEII BstZ17I ClaI EagI EciI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PshAI PspXI PvuI RsrII SacII SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XcmI XhoI ZraI