Restriction map for
pFN21AB0984
(Linear) (Six-base) MAPSORT of: KIBB0984.seq Check: 261 from: 1 to: 1356
KIBB0984.orf
With 182 enzymes: SgfI *
July 3, 2012 16:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1356
Size: 718 638
AgeI A'CCGG_T
Cuts at: 0 362 1356
Size: 362 994
AleI CACnn'nnGTG
Cuts at: 0 761 1356
Size: 761 595
AlwNI CAG_nnn'CTG
Cuts at: 0 245 500 795 1356
Size: 245 255 295 561
Fragments arranged by size:
561 295 255 245
ApaI G_GGCC'C
Cuts at: 0 821 1356
Size: 821 535
ApoI r'AATT_y
Cuts at: 0 3 516 620 911 1356
Size: 3 513 104 291 445
Fragments arranged by size:
513 445 291 104 3
AvaI C'yCGr_G
Cuts at: 0 1011 1356
Size: 1011 345
BanII G_rGCy'C
Cuts at: 0 262 809 821 1356
Size: 262 547 12 535
Fragments arranged by size:
547 535 262 12
BbvCI CC'TCA_GC
Cuts at: 0 398 1356
Size: 398 958
BglI GCCn_nnn'nGGC
Cuts at: 0 655 1356
Size: 655 701
Bme1580I G_kGCm'C
Cuts at: 0 95 821 1356
Size: 95 726 535
Fragments arranged by size:
726 535 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 1035 1356
Size: 1035 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1356
Size: 330 1026
Bpu10I CC'TnA_GC
Cuts at: 0 256 398 1356
Size: 256 142 958
Fragments arranged by size:
958 256 142
BsaBI GATnn'nnATC
Cuts at: 0 1274 1356
Size: 1274 82
BsaWI w'CCGG_w
Cuts at: 0 362 1356
Size: 362 994
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 349 379 1356
Size: 259 30 60 30 977
Fragments arranged by size:
977 259 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 706 1313 1356
Size: 280 426 607 43
Fragments arranged by size:
607 426 280 43
BseYI C'CCAG_C
Cuts at: 0 788 1356
Size: 788 568
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 833 1356
Size: 763 70 523
Fragments arranged by size:
763 523 70
BsiHKAI G_wGCw'C
Cuts at: 0 157 262 346 362 809 1356
Size: 157 105 84 16 447 547
Fragments arranged by size:
547 447 157 105 84 16
BsmI GAATG_Cn'
Cuts at: 0 166 1356
Size: 166 1190
Bsp1286I G_dGCh'C
Cuts at: 0 95 157 262 346 362 809 821 1356
Size: 95 62 105 84 16 447 12 535
Fragments arranged by size:
535 447 105 95 84 62 16 12
BspHI T'CATG_A
Cuts at: 0 570 876 1356
Size: 570 306 480
Fragments arranged by size:
570 480 306
BsrFI r'CCGG_y
Cuts at: 0 362 1356
Size: 362 994
BstEII G'GTnAC_C
Cuts at: 0 75 1356
Size: 75 1281
BtsI GCAGTG_nn'
Cuts at: 0 962 1356
Size: 962 394
EcoICRI GAG'CTC
Cuts at: 0 260 807 1356
Size: 260 547 549
Fragments arranged by size:
549 547 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 718 1356
Size: 330 388 638
Fragments arranged by size:
638 388 330
EcoO109I rG'GnC_Cy
Cuts at: 0 817 1356
Size: 817 539
EcoRI G'AATT_C
Cuts at: 0 3 1356
Size: 3 1353
EcoRV GAT'ATC
Cuts at: 0 897 1356
Size: 897 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 207 239 1356
Size: 207 32 1117
Fragments arranged by size:
1117 207 32
HincII GTy'rAC
Cuts at: 0 173 1356
Size: 173 1183
HindIII A'AGCT_T
Cuts at: 0 415 449 1356
Size: 415 34 907
Fragments arranged by size:
907 415 34
MslI CAynn'nnrTG
Cuts at: 0 761 947 1356
Size: 761 186 409
Fragments arranged by size:
761 409 186
NspI r_CATG'y
Cuts at: 0 786 927 1356
Size: 786 141 429
Fragments arranged by size:
786 429 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 389 500 1356
Size: 389 111 856
Fragments arranged by size:
856 389 111
PfoI T'CCnGG_A
Cuts at: 0 1048 1235 1356
Size: 1048 187 121
Fragments arranged by size:
1048 187 121
PspOMI G'GGCC_C
Cuts at: 0 817 1356
Size: 817 539
SacI G_AGCT'C
Cuts at: 0 262 809 1356
Size: 262 547 547
Fragments arranged by size:
547 547 262
SspI AAT'ATT
Cuts at: 0 1021 1356
Size: 1021 335
StuI AGG'CCT
Cuts at: 0 108 1332 1356
Size: 108 1224 24
Fragments arranged by size:
1224 108 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1130 1356
Size: 1130 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 1356
Size: 259 32 1065
Fragments arranged by size:
1065 259 32
Tth111I GACn'n_nGTC
Cuts at: 0 73 377 1356
Size: 73 304 979
Fragments arranged by size:
979 304 73
XmnI GAAnn'nnTTC
Cuts at: 0 61 304 623 1356
Size: 61 243 319 733
Fragments arranged by size:
733 319 243 61
Enzymes that do cut:
AcuI AgeI AleI AlwNI ApaI ApoI AvaI BanII
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrFI BstEII BtsI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HincII HindIII MslI NspI PflMI PfoI PspOMI
SacI SspI StuI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoNI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StyI SwaI TatI
XbaI XcmI XhoI XmaI ZraI