Restriction map for
pFN21AB0932
(Linear) (Six-base) MAPSORT of: KIBB0932.seq Check: 3646 from: 1 to: 624
KIBB0932.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 49 624
Size: 49 575
AfeI AGC'GCT
Cuts at: 0 575 624
Size: 575 49
AgeI A'CCGG_T
Cuts at: 0 362 624
Size: 362 262
AlwNI CAG_nnn'CTG
Cuts at: 0 110 245 377 500 564 624
Size: 110 135 132 123 64 60
Fragments arranged by size:
135 132 123 110 64 60
ApaI G_GGCC'C
Cuts at: 0 590 624
Size: 590 34
ApoI r'AATT_y
Cuts at: 0 3 624
Size: 3 621
BanII G_rGCy'C
Cuts at: 0 262 590 624
Size: 262 328 34
Fragments arranged by size:
328 262 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 15 624
Size: 15 609
BbvCI CC'TCA_GC
Cuts at: 0 398 624
Size: 398 226
BfrBI ATG'CAT
Cuts at: 0 164 624
Size: 164 460
Bme1580I G_kGCm'C
Cuts at: 0 95 590 624
Size: 95 495 34
Fragments arranged by size:
495 95 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 417 624
Size: 417 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 624
Size: 330 294
Bpu10I CC'TnA_GC
Cuts at: 0 256 398 577 624
Size: 256 142 179 47
Fragments arranged by size:
256 179 142 47
BsaWI w'CCGG_w
Cuts at: 0 362 624
Size: 362 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 349 379 403 433 624
Size: 259 30 60 30 24 30 191
Fragments arranged by size:
259 191 60 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 624
Size: 280 344
BseYI C'CCAG_C
Cuts at: 0 557 624
Size: 557 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 624
Size: 532 92
BsiHKAI G_wGCw'C
Cuts at: 0 262 346 362 624
Size: 262 84 16 262
Fragments arranged by size:
262 262 84 16
BsmI GAATG_Cn'
Cuts at: 0 166 281 624
Size: 166 115 343
Fragments arranged by size:
343 166 115
Bsp1286I G_dGCh'C
Cuts at: 0 95 262 346 362 590 624
Size: 95 167 84 16 228 34
Fragments arranged by size:
228 167 95 84 34 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 624
Size: 49 575
BsrFI r'CCGG_y
Cuts at: 0 362 624
Size: 362 262
BstEII G'GTnAC_C
Cuts at: 0 75 624
Size: 75 549
EcoICRI GAG'CTC
Cuts at: 0 260 624
Size: 260 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 624
Size: 330 294
EcoO109I rG'GnC_Cy
Cuts at: 0 107 586 624
Size: 107 479 38
Fragments arranged by size:
479 107 38
EcoRI G'AATT_C
Cuts at: 0 3 624
Size: 3 621
HaeII r_GCGC'y
Cuts at: 0 577 624
Size: 577 47
HindIII A'AGCT_T
Cuts at: 0 449 624
Size: 449 175
MslI CAynn'nnrTG
Cuts at: 0 52 624
Size: 52 572
MspA1I CmG'CkG
Cuts at: 0 385 624
Size: 385 239
NsiI A_TGCA'T
Cuts at: 0 166 624
Size: 166 458
NspI r_CATG'y
Cuts at: 0 555 624
Size: 555 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 245 624
Size: 245 379
PspOMI G'GGCC_C
Cuts at: 0 586 624
Size: 586 38
SacI G_AGCT'C
Cuts at: 0 262 624
Size: 262 362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 624
Size: 259 32 333
Fragments arranged by size:
333 259 32
Tth111I GACn'n_nGTC
Cuts at: 0 73 377 624
Size: 73 304 247
Fragments arranged by size:
304 247 73
XmnI GAAnn'nnTTC
Cuts at: 0 61 304 624
Size: 61 243 320
Fragments arranged by size:
320 243 61
Enzymes that do cut:
AarI AfeI AgeI AlwNI ApaI ApoI BanII BbsI
BbvCI BfrBI Bme1580I BmrI BpmI Bpu10I BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BstEII EcoICRI Eco57MI EcoO109I EcoRI HaeII HindIII MslI
MspA1I NsiI NspI PflMI PspOMI SacI TstI Tth111I
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoNI
EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI XbaI XcmI
XhoI XmaI ZraI