Restriction map for
pFN21AB0486
(Linear) (Six-base) MAPSORT of: KIBB0486.seq Check: 6322 from: 1 to: 3090
KIBB0486.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1126 3090
Size: 1126 1964
AccI GT'mk_AC
Cuts at: 0 452 3090
Size: 452 2638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 901 1360 1504 1545 2594 2794 3090
Size: 316 585 459 144 41 1049 200 296
Fragments arranged by size:
1049 585 459 316 296 200 144 41
AflIII A'CryG_T
Cuts at: 0 2063 3090
Size: 2063 1027
AhdI GACnn_n'nnGTC
Cuts at: 0 448 3090
Size: 448 2642
AleI CACnn'nnGTG
Cuts at: 0 1079 2548 2925 2959 3090
Size: 1079 1469 377 34 131
Fragments arranged by size:
1469 1079 377 131 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1148 1195 2566 2943 3090
Size: 1148 47 1371 377 147
Fragments arranged by size:
1371 1148 377 147 47
AlwNI CAG_nnn'CTG
Cuts at: 0 426 843 2319 2453 2512 3090
Size: 426 417 1476 134 59 578
Fragments arranged by size:
1476 578 426 417 134 59
ApaI G_GGCC'C
Cuts at: 0 91 2378 3090
Size: 91 2287 712
Fragments arranged by size:
2287 712 91
ApaLI G'TGCA_C
Cuts at: 0 58 3090
Size: 58 3032
ApoI r'AATT_y
Cuts at: 0 1614 2326 3090
Size: 1614 712 764
Fragments arranged by size:
1614 764 712
AvaI C'yCGr_G
Cuts at: 0 191 482 1148 2091 3090
Size: 191 291 666 943 999
Fragments arranged by size:
999 943 666 291 191
AvrII C'CTAG_G
Cuts at: 0 504 3090
Size: 504 2586
BamHI G'GATC_C
Cuts at: 0 555 3090
Size: 555 2535
BanI G'GyrC_C
Cuts at: 0 30 40 1070 1411 3090
Size: 30 10 1030 341 1679
Fragments arranged by size:
1679 1030 341 30 10
BanII G_rGCy'C
Cuts at: 0 91 1637 1922 2008 2371 2378 3082 3090
Size: 91 1546 285 86 363 7 704 8
Fragments arranged by size:
1546 704 363 285 91 86 8 7
BbeI G_GCGC'C
Cuts at: 0 34 3090
Size: 34 3056
BbsI GAAGACnn'nnnn_
Cuts at: 0 722 724 1295 2839 3090
Size: 722 2 571 1544 251
Fragments arranged by size:
1544 722 571 251 2
BbvCI CC'TCA_GC
Cuts at: 0 1009 1022 1589 2195 2800 2884 3090
Size: 1009 13 567 606 605 84 206
Fragments arranged by size:
1009 606 605 567 206 84 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1554 1588 3090
Size: 1554 34 1502
Fragments arranged by size:
1554 1502 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 715 3090
Size: 715 2375
BfrBI ATG'CAT
Cuts at: 0 2934 3090
Size: 2934 156
BglI GCCn_nnn'nGGC
Cuts at: 0 372 3090
Size: 372 2718
BglII A'GATC_T
Cuts at: 0 1422 3090
Size: 1422 1668
Bme1580I G_kGCm'C
Cuts at: 0 45 62 91 198 1416 2045 2378 3090
Size: 45 17 29 107 1218 629 333 712
Fragments arranged by size:
1218 712 629 333 107 45 29 17
BmtI G_CTAG'C
Cuts at: 0 2341 3090
Size: 2341 749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 736 3090
Size: 736 2354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 588 2776 3090
Size: 271 317 2188 314
Fragments arranged by size:
2188 317 314 271
Bpu10I CC'TnA_GC
Cuts at: 0 1009 1022 1045 1589 2002 2195 2800 2884
Size: 1009 13 23 544 413 193 605 84
Cuts at: 2884 2997 3090
Size: 113 93
Fragments arranged by size:
1009 605 544 413 193 113 93 84
23 13
BsaAI yAC'GTr
Cuts at: 0 2853 3090
Size: 2853 237
BsaHI Gr'CG_yC
Cuts at: 0 31 3090
Size: 31 3059
BsaWI w'CCGG_w
Cuts at: 0 1086 2909 3090
Size: 1086 1823 181
Fragments arranged by size:
1823 1086 181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 616 625 655 2749 2779 3090
Size: 586 30 9 30 2094 30 311
Fragments arranged by size:
2094 586 311 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 1606 2112 2281 3090
Size: 649 957 506 169 809
Fragments arranged by size:
957 809 649 506 169
BseYI C'CCAG_C
Cuts at: 0 895 1363 1370 1415 1696 3090
Size: 895 468 7 45 281 1394
Fragments arranged by size:
1394 895 468 281 45 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1915 2526 2806 3090
Size: 1915 611 280 284
Fragments arranged by size:
1915 611 284 280
BsiHKAI G_wGCw'C
Cuts at: 0 62 1336 2432 3090
Size: 62 1274 1096 658
Fragments arranged by size:
1274 1096 658 62
BsmI GAATG_Cn'
Cuts at: 0 852 3090
Size: 852 2238
Bsp1286I G_dGCh'C
Cuts at: 0 45 62 91 198 1336 1416 1637 1922
Size: 45 17 29 107 1138 80 221 285
Cuts at: 1922 2008 2045 2371 2378 2432 3082 3090
Size: 86 37 326 7 54 650 8
Fragments arranged by size:
1138 650 326 285 221 107 86 80
54 45 37 29 17 8 7
BspEI T'CCGG_A
Cuts at: 0 2909 3090
Size: 2909 181
BspHI T'CATG_A
Cuts at: 0 2840 3090
Size: 2840 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1126 3090
Size: 1126 1964
BsrBI CCG'CTC
Cuts at: 0 945 3090
Size: 945 2145
BsrDI GCAATG_nn'
Cuts at: 0 1179 3090
Size: 1179 1911
BsrFI r'CCGG_y
Cuts at: 0 290 3090
Size: 290 2800
BsrGI T'GTAC_A
Cuts at: 0 417 1466 3090
Size: 417 1049 1624
Fragments arranged by size:
1624 1049 417
BssSI C'ACGA_G
Cuts at: 0 55 197 2558 3090
Size: 55 142 2361 532
Fragments arranged by size:
2361 532 142 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2931 3090
Size: 2931 159
BstEII G'GTnAC_C
Cuts at: 0 1459 3090
Size: 1459 1631
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3009 3090
Size: 3009 81
BstYI r'GATC_y
Cuts at: 0 555 1422 3090
Size: 555 867 1668
Fragments arranged by size:
1668 867 555
Bsu36I CC'TnA_GG
Cuts at: 0 2124 3090
Size: 2124 966
BtgI C'CryG_G
Cuts at: 0 11 699 1967 2900 3090
Size: 11 688 1268 933 190
Fragments arranged by size:
1268 933 688 190 11
BtsI GCAGTG_nn'
Cuts at: 0 1460 1714 1985 2146 2920 2932 3050 3090
Size: 1460 254 271 161 774 12 118 40
Fragments arranged by size:
1460 774 271 254 161 118 40 12
ClaI AT'CG_AT
Cuts at: 0 671 3090
Size: 671 2419
DraIII CAC_nnn'GTG
Cuts at: 0 2040 3090
Size: 2040 1050
DrdI GACnn_nn'nnGTC
Cuts at: 0 2863 3090
Size: 2863 227
EaeI y'GGCC_r
Cuts at: 0 2 2565 3090
Size: 2 2563 525
Fragments arranged by size:
2563 525 2
EarI CTCTTCn'nnn_
Cuts at: 0 485 2053 2405 2822 3016 3090
Size: 485 1568 352 417 194 74
Fragments arranged by size:
1568 485 417 352 194 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1245 2581 3090
Size: 1245 1336 509
Fragments arranged by size:
1336 1245 509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 316 588 901 1360 1504 1545 2594
Size: 271 45 272 313 459 144 41 1049
Cuts at: 2594 2776 2794 3090
Size: 182 18 296
Fragments arranged by size:
1049 459 313 296 272 271 182 144
45 41 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 430 1486 3090
Size: 253 177 1056 1604
Fragments arranged by size:
1604 1056 253 177
EcoO109I rG'GnC_Cy
Cuts at: 0 51 87 88 161 622 960 1026 1833
Size: 51 36 1 73 461 338 66 807
Cuts at: 1833 2375 2421 2760 3090
Size: 542 46 339 330
Fragments arranged by size:
807 542 461 339 338 330 73 66
51 46 36 1
EcoRI G'AATT_C
Cuts at: 0 2326 3090
Size: 2326 764
FspI TGC'GCA
Cuts at: 0 3034 3090
Size: 3034 56
HaeII r_GCGC'y
Cuts at: 0 34 3090
Size: 34 3056
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 434 466 586 618 1414 1446
Size: 103 32 299 32 120 32 796 32
Cuts at: 1446 1704 1736 2107 2139 2506 2538 2749 2781
Size: 258 32 371 32 367 32 211 32
Cuts at: 2781 3090
Size: 309
Fragments arranged by size:
796 371 367 309 299 258 211 120
103 32 32 32 32 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 30 3090
Size: 30 3060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 3090
Size: 806 2284
MscI TGG'CCA
Cuts at: 0 2567 3090
Size: 2567 523
MslI CAynn'nnrTG
Cuts at: 0 535 1079 2548 2925 2959 3090
Size: 535 544 1469 377 34 131
Fragments arranged by size:
1469 544 535 377 131 34
MspA1I CmG'CkG
Cuts at: 0 7 13 94 1132 1227 1329 1681 2199
Size: 7 6 81 1038 95 102 352 518
Cuts at: 2199 2246 2266 2319 2512 2598 2804 3090
Size: 47 20 53 193 86 206 286
Fragments arranged by size:
1038 518 352 286 206 193 102 95
86 81 53 47 20 7 6
NarI GG'CG_CC
Cuts at: 0 31 3090
Size: 31 3059
NcoI C'CATG_G
Cuts at: 0 699 1967 2900 3090
Size: 699 1268 933 190
Fragments arranged by size:
1268 933 699 190
NheI G'CTAG_C
Cuts at: 0 2337 3090
Size: 2337 753
NsiI A_TGCA'T
Cuts at: 0 2936 3090
Size: 2936 154
NspI r_CATG'y
Cuts at: 0 418 2934 2938 3090
Size: 418 2516 4 152
Fragments arranged by size:
2516 418 152 4
PasI CC'CwG_GG
Cuts at: 0 370 3090
Size: 370 2720
PflMI CCAn_nnn'nTGG
Cuts at: 0 540 902 3090
Size: 540 362 2188
Fragments arranged by size:
2188 540 362
PfoI T'CCnGG_A
Cuts at: 0 248 404 2111 3090
Size: 248 156 1707 979
Fragments arranged by size:
1707 979 248 156
PmlI CAC'GTG
Cuts at: 0 2853 3090
Size: 2853 237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2872 2904 3090
Size: 2872 32 186
Fragments arranged by size:
2872 186 32
PpuMI rG'GwC_Cy
Cuts at: 0 161 622 1026 1833 3090
Size: 161 461 404 807 1257
Fragments arranged by size:
1257 807 461 404 161
PspOMI G'GGCC_C
Cuts at: 0 87 2374 3090
Size: 87 2287 716
Fragments arranged by size:
2287 716 87
PstI C_TGCA'G
Cuts at: 0 111 332 1069 1137 1271 1733 2310 2927
Size: 111 221 737 68 134 462 577 617
Cuts at: 2927 3090
Size: 163
Fragments arranged by size:
737 617 577 462 221 163 134 111
68
PvuII CAG'CTG
Cuts at: 0 1132 1329 1681 2199 2246 2319 2512 2804
Size: 1132 197 352 518 47 73 193 292
Cuts at: 2804 3090
Size: 286
Fragments arranged by size:
1132 518 352 292 286 197 193 73
47
SacII CC_GC'GG
Cuts at: 0 14 3090
Size: 14 3076
SanDI GG'GwC_CC
Cuts at: 0 622 3090
Size: 622 2468
SapI GCTCTTCn'nnn_
Cuts at: 0 485 3090
Size: 485 2605
SbfI CC_TGCA'GG
Cuts at: 0 332 2310 3090
Size: 332 1978 780
Fragments arranged by size:
1978 780 332
ScaI AGT'ACT
Cuts at: 0 1940 3090
Size: 1940 1150
SexAI A'CCwGG_T
Cuts at: 0 98 3090
Size: 98 2992
SfcI C'TryA_G
Cuts at: 0 107 328 612 835 1057 1065 1133 1267
Size: 107 221 284 223 222 8 68 134
Cuts at: 1267 1729 2306 2332 2923 3090
Size: 462 577 26 591 167
Fragments arranged by size:
591 577 462 284 223 222 221 167
134 107 68 26 8
SfoI GGC'GCC
Cuts at: 0 32 3090
Size: 32 3058
SmaI CCC'GGG
Cuts at: 0 484 1150 3090
Size: 484 666 1940
Fragments arranged by size:
1940 666 484
SphI G_CATG'C
Cuts at: 0 2934 2938 3090
Size: 2934 4 152
Fragments arranged by size:
2934 152 4
SrfI GCCC'GGGC
Cuts at: 0 1150 3090
Size: 1150 1940
StuI AGG'CCT
Cuts at: 0 1305 1900 2392 3090
Size: 1305 595 492 698
Fragments arranged by size:
1305 698 595 492
StyI C'CwwG_G
Cuts at: 0 156 504 699 1886 1967 2900 3090
Size: 156 348 195 1187 81 933 190
Fragments arranged by size:
1187 933 348 195 190 156 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 28 455 1384 1399 1654 3090
Size: 28 427 929 15 255 1436
Fragments arranged by size:
1436 929 427 255 28 15
TatI w'GTAC_w
Cuts at: 0 417 1176 1466 1938 2691 2715 3090
Size: 417 759 290 472 753 24 375
Fragments arranged by size:
759 753 472 417 375 290 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 188 220 614 646 1315 1347 3090
Size: 188 32 394 32 669 32 1743
Fragments arranged by size:
1743 669 394 188 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 67 1162 3090
Size: 67 1095 1928
Fragments arranged by size:
1928 1095 67
XbaI T'CTAG_A
Cuts at: 0 168 3090
Size: 168 2922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 541 3090
Size: 541 2549
XmaI C'CCGG_G
Cuts at: 0 482 1148 3090
Size: 482 666 1942
Fragments arranged by size:
1942 666 482
XmnI GAAnn'nnTTC
Cuts at: 0 886 1315 3090
Size: 886 429 1775
Fragments arranged by size:
1775 886 429
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII
Bme1580I BmtI BplI BpmI Bpu10I BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NcoI NheI NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacII SanDI
SapI SbfI ScaI SexAI SfcI SfoI SmaI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI AscI
AseI AsiSI BaeI BclI BlpI BmgBI BmrI BpuEI
BsaI BsaBI BsiEI BsiWI BsmBI BssHII BstBI BstZ17I
BtgZI CspCI DraI EagI EcoICRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NotI NruI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SfiI
SgfI SgrAI SmlI SnaBI SpeI SspI SwaI XhoI
ZraI