Restriction map for
pFN21AB0483
(Linear) (Six-base) MAPSORT of: KIBB0483.seq Check: 5534 from: 1 to: 1578
KIBB0483.orf
With 182 enzymes: SgfI *
July 3, 2012 16:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 461 1578
Size: 148 313 1117
Fragments arranged by size:
1117 313 148
AhdI GACnn_n'nnGTC
Cuts at: 0 180 991 1578
Size: 180 811 587
Fragments arranged by size:
811 587 180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 203 235 848 880 1578
Size: 203 32 613 32 698
Fragments arranged by size:
698 613 203 32 32
ApaI G_GGCC'C
Cuts at: 0 1421 1578
Size: 1421 157
ApaLI G'TGCA_C
Cuts at: 0 151 1578
Size: 151 1427
ApoI r'AATT_y
Cuts at: 0 1248 1578
Size: 1248 330
AseI AT'TA_AT
Cuts at: 0 821 1376 1578
Size: 821 555 202
Fragments arranged by size:
821 555 202
AvaI C'yCGr_G
Cuts at: 0 1303 1444 1578
Size: 1303 141 134
Fragments arranged by size:
1303 141 134
AvrII C'CTAG_G
Cuts at: 0 1539 1578
Size: 1539 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 61 94 1578
Size: 61 33 1484
Fragments arranged by size:
1484 61 33
BanI G'GyrC_C
Cuts at: 0 1127 1578
Size: 1127 451
BanII G_rGCy'C
Cuts at: 0 91 882 1421 1578
Size: 91 791 539 157
Fragments arranged by size:
791 539 157 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 1335 1578
Size: 176 1159 243
Fragments arranged by size:
1159 243 176
BfrBI ATG'CAT
Cuts at: 0 427 1578
Size: 427 1151
BglII A'GATC_T
Cuts at: 0 461 1578
Size: 461 1117
BlpI GC'TnA_GC
Cuts at: 0 42 1578
Size: 42 1536
Bme1580I G_kGCm'C
Cuts at: 0 155 1421 1578
Size: 155 1266 157
Fragments arranged by size:
1266 157 155
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 1371 1578
Size: 22 1349 207
Fragments arranged by size:
1349 207 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 250 1578
Size: 250 1328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 832 1210 1288 1578
Size: 146 686 378 78 290
Fragments arranged by size:
686 378 290 146 78
Bpu10I CC'TnA_GC
Cuts at: 0 482 1578
Size: 482 1096
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 235 265 369 399 1578
Size: 124 30 81 30 104 30 1179
Fragments arranged by size:
1179 124 104 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 132 390 497 715 1578
Size: 132 258 107 218 863
Fragments arranged by size:
863 258 218 132 107
BseYI C'CCAG_C
Cuts at: 0 121 1578
Size: 121 1457
BsiHKAI G_wGCw'C
Cuts at: 0 155 1578
Size: 155 1423
BsmI GAATG_Cn'
Cuts at: 0 498 924 1578
Size: 498 426 654
Fragments arranged by size:
654 498 426
Bsp1286I G_dGCh'C
Cuts at: 0 91 155 882 1421 1578
Size: 91 64 727 539 157
Fragments arranged by size:
727 539 157 91 64
BspHI T'CATG_A
Cuts at: 0 365 1578
Size: 365 1213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 929 1578
Size: 929 649
BsrBI CCG'CTC
Cuts at: 0 723 1578
Size: 723 855
BsrFI r'CCGG_y
Cuts at: 0 136 1578
Size: 136 1442
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 1578
Size: 912 666
BstYI r'GATC_y
Cuts at: 0 461 1578
Size: 461 1117
Bsu36I CC'TnA_GG
Cuts at: 0 239 473 1082 1578
Size: 239 234 609 496
Fragments arranged by size:
609 496 239 234
BtgI C'CryG_G
Cuts at: 0 146 874 1562 1578
Size: 146 728 688 16
Fragments arranged by size:
728 688 146 16
EarI CTCTTCn'nnn_
Cuts at: 0 1451 1578
Size: 1451 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 148 461 832 1210 1288 1578
Size: 146 2 313 371 378 78 290
Fragments arranged by size:
378 371 313 290 146 78 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1267 1282 1578
Size: 1267 15 296
Fragments arranged by size:
1267 296 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1272 1578
Size: 1272 306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1335 1578
Size: 1335 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 166 198 235 267 369 401
Size: 14 32 120 32 37 32 102 32
Cuts at: 401 977 1009 1320 1352 1578
Size: 576 32 311 32 226
Fragments arranged by size:
576 311 226 120 102 37 32 32
32 32 32 32 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1578
Size: 698 880
MslI CAynn'nnrTG
Cuts at: 0 258 1578
Size: 258 1320
MspA1I CmG'CkG
Cuts at: 0 1192 1230 1578
Size: 1192 38 348
Fragments arranged by size:
1192 348 38
NcoI C'CATG_G
Cuts at: 0 146 1578
Size: 146 1432
NsiI A_TGCA'T
Cuts at: 0 429 1578
Size: 429 1149
NspI r_CATG'y
Cuts at: 0 203 217 431 1578
Size: 203 14 214 1147
Fragments arranged by size:
1147 214 203 14
PacI TTA_AT'TAA
Cuts at: 0 1358 1578
Size: 1358 220
PfoI T'CCnGG_A
Cuts at: 0 866 1265 1578
Size: 866 399 313
Fragments arranged by size:
866 399 313
PspOMI G'GGCC_C
Cuts at: 0 1417 1578
Size: 1417 161
PstI C_TGCA'G
Cuts at: 0 1283 1578
Size: 1283 295
PvuII CAG'CTG
Cuts at: 0 1192 1230 1578
Size: 1192 38 348
Fragments arranged by size:
1192 348 38
SapI GCTCTTCn'nnn_
Cuts at: 0 1451 1578
Size: 1451 127
SfcI C'TryA_G
Cuts at: 0 1279 1578
Size: 1279 299
SphI G_CATG'C
Cuts at: 0 431 1578
Size: 431 1147
SspI AAT'ATT
Cuts at: 0 966 1242 1578
Size: 966 276 336
Fragments arranged by size:
966 336 276
StyI C'CwwG_G
Cuts at: 0 146 537 911 1310 1539 1578
Size: 146 391 374 399 229 39
Fragments arranged by size:
399 391 374 229 146 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 196 266 1578
Size: 196 70 1312
Fragments arranged by size:
1312 196 70
TatI w'GTAC_w
Cuts at: 0 131 800 1578
Size: 131 669 778
Fragments arranged by size:
778 669 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1578
Size: 1111 32 435
Fragments arranged by size:
1111 435 32
Tth111I GACn'n_nGTC
Cuts at: 0 1495 1578
Size: 1495 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 338 1578
Size: 338 1240
XmnI GAAnn'nnTTC
Cuts at: 0 22 1578
Size: 22 1556
Enzymes that do cut:
AcuI AhdI AloI ApaI ApaLI ApoI AseI AvaI
AvrII BaeI BanI BanII BbsI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BstXI
BstYI Bsu36I BtgI EarI Eco57MI EcoNI EcoO109I FalI
Hin4I MmeI MslI MspA1I NcoI NsiI NspI PacI
PfoI PspOMI PstI PvuII SapI SfcI SphI SspI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AlwNI AscI AsiSI BamHI BbeI
BbvCI BcgI BciVI BclI BglI BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI
SwaI XbaI XhoI XmaI ZraI