Restriction map for
pFN21AB0461
(Linear) (Six-base) MAPSORT of: KIBB0461.seq Check: 7755 from: 1 to: 1329
KIBB0461.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AccI GT'mk_AC
Cuts at: 0 325 1217 1329
Size: 325 892 112
Fragments arranged by size:
892 325 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1068 1250 1329
Size: 268 800 182 79
Fragments arranged by size:
800 268 182 79
AflIII A'CryG_T
Cuts at: 0 563 1329
Size: 563 766
AleI CACnn'nnGTG
Cuts at: 0 667 712 1329
Size: 667 45 617
Fragments arranged by size:
667 617 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 847 879 1329
Size: 847 32 450
Fragments arranged by size:
847 450 32
ApoI r'AATT_y
Cuts at: 0 91 787 1021 1329
Size: 91 696 234 308
Fragments arranged by size:
696 308 234 91
AvaI C'yCGr_G
Cuts at: 0 232 1151 1329
Size: 232 919 178
Fragments arranged by size:
919 232 178
BamHI G'GATC_C
Cuts at: 0 420 1329
Size: 420 909
BanI G'GyrC_C
Cuts at: 0 447 1329
Size: 447 882
BanII G_rGCy'C
Cuts at: 0 662 1329
Size: 662 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 727 1329
Size: 693 34 602
Fragments arranged by size:
693 602 34
BclI T'GATC_A
Cuts at: 0 671 1329
Size: 671 658
BglII A'GATC_T
Cuts at: 0 1241 1329
Size: 1241 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1329
Size: 1144 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 453 1329
Size: 453 876
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 1329
Size: 514 30 785
Fragments arranged by size:
785 514 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 418 670 1329
Size: 19 399 252 659
Fragments arranged by size:
659 399 252 19
BseYI C'CCAG_C
Cuts at: 0 296 1329
Size: 296 1033
BsiEI CG_ry'CG
Cuts at: 0 65 1329
Size: 65 1264
BsiHKAI G_wGCw'C
Cuts at: 0 662 1329
Size: 662 667
BsmI GAATG_Cn'
Cuts at: 0 309 1329
Size: 309 1020
Bsp1286I G_dGCh'C
Cuts at: 0 662 1329
Size: 662 667
BspHI T'CATG_A
Cuts at: 0 887 963 1058 1329
Size: 887 76 95 271
Fragments arranged by size:
887 271 95 76
BsrDI GCAATG_nn'
Cuts at: 0 699 1329
Size: 699 630
BsrFI r'CCGG_y
Cuts at: 0 410 1329
Size: 410 919
BsrGI T'GTAC_A
Cuts at: 0 716 1329
Size: 716 613
BstYI r'GATC_y
Cuts at: 0 420 807 921 1241 1329
Size: 420 387 114 320 88
Fragments arranged by size:
420 387 320 114 88
Bsu36I CC'TnA_GG
Cuts at: 0 580 1329
Size: 580 749
BtgI C'CryG_G
Cuts at: 0 464 1329
Size: 464 865
BtsI GCAGTG_nn'
Cuts at: 0 1289 1329
Size: 1289 40
EarI CTCTTCn'nnn_
Cuts at: 0 244 489 791 849 1329
Size: 244 245 302 58 480
Fragments arranged by size:
480 302 245 244 58
EcoICRI GAG'CTC
Cuts at: 0 660 1329
Size: 660 669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1068 1250 1329
Size: 268 800 182 79
Fragments arranged by size:
800 268 182 79
EcoRI G'AATT_C
Cuts at: 0 91 1329
Size: 91 1238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 159 191 1329
Size: 64 32 63 32 1138
Fragments arranged by size:
1138 64 63 32 32
HincII GTy'rAC
Cuts at: 0 543 1218 1326 1329
Size: 543 675 108 3
Fragments arranged by size:
675 543 108 3
HpaI GTT'AAC
Cuts at: 0 1326 1329
Size: 1326 3
MslI CAynn'nnrTG
Cuts at: 0 56 667 712 1329
Size: 56 611 45 617
Fragments arranged by size:
617 611 56 45
MspA1I CmG'CkG
Cuts at: 0 571 1329
Size: 571 758
NaeI GCC'GGC
Cuts at: 0 412 1329
Size: 412 917
NcoI C'CATG_G
Cuts at: 0 464 1329
Size: 464 865
NgoMIV G'CCGG_C
Cuts at: 0 410 1329
Size: 410 919
NspI r_CATG'y
Cuts at: 0 567 756 1329
Size: 567 189 573
Fragments arranged by size:
573 567 189
PciI A'CATG_T
Cuts at: 0 563 1329
Size: 563 766
PfoI T'CCnGG_A
Cuts at: 0 416 1313 1329
Size: 416 897 16
Fragments arranged by size:
897 416 16
SacI G_AGCT'C
Cuts at: 0 662 1329
Size: 662 667
SalI G'TCGA_C
Cuts at: 0 1216 1329
Size: 1216 113
SapI GCTCTTCn'nnn_
Cuts at: 0 791 1329
Size: 791 538
SexAI A'CCwGG_T
Cuts at: 0 956 1329
Size: 956 373
SmaI CCC'GGG
Cuts at: 0 1153 1329
Size: 1153 176
SmlI C'TyrA_G
Cuts at: 0 1123 1329
Size: 1123 206
SspI AAT'ATT
Cuts at: 0 149 627 1275 1329
Size: 149 478 648 54
Fragments arranged by size:
648 478 149 54
StyI C'CwwG_G
Cuts at: 0 464 1329
Size: 464 865
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 1329
Size: 79 1250
TatI w'GTAC_w
Cuts at: 0 716 1329
Size: 716 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 1329
Size: 514 32 783
Fragments arranged by size:
783 514 32
Tth111I GACn'n_nGTC
Cuts at: 0 1051 1329
Size: 1051 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 1329
Size: 471 858
XmaI C'CCGG_G
Cuts at: 0 1151 1329
Size: 1151 178
Enzymes that do cut:
AccI AcuI AflIII AleI AloI ApoI AvaI BamHI
BanI BanII BcgI BclI BglII BpuEI BsaI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BsrFI BsrGI BstYI Bsu36I BtgI BtsI EarI EcoICRI
Eco57MI EcoRI Hin4I HincII HpaI MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PfoI SacI SalI SapI
SexAI SmaI SmlI SspI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsgI BsiWI BsmBI BspEI BspMI
BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI MmeI MscI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI StuI SwaI XbaI XhoI XmnI
ZraI