Restriction map for
pFN21AB0460
(Linear) (Six-base) MAPSORT of: KIBB0460.seq Check: 1845 from: 1 to: 2898
KIBB0460.orf
With 182 enzymes: SgfI *
March 22, 2012 15:30 ..
AccI GT'mk_AC
Cuts at: 0 770 1338 2898
Size: 770 568 1560
Fragments arranged by size:
1560 770 568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 238 359 1058 2182 2819 2898
Size: 36 202 121 699 1124 637 79
Fragments arranged by size:
1124 699 637 202 121 79 36
AfeI AGC'GCT
Cuts at: 0 2399 2898
Size: 2399 499
AflII C'TTAA_G
Cuts at: 0 411 2898
Size: 411 2487
AgeI A'CCGG_T
Cuts at: 0 1343 2898
Size: 1343 1555
AhdI GACnn_n'nnGTC
Cuts at: 0 2664 2898
Size: 2664 234
AleI CACnn'nnGTG
Cuts at: 0 199 1681 2898
Size: 199 1482 1217
Fragments arranged by size:
1482 1217 199
AlwNI CAG_nnn'CTG
Cuts at: 0 519 2348 2522 2898
Size: 519 1829 174 376
Fragments arranged by size:
1829 519 376 174
ApaLI G'TGCA_C
Cuts at: 0 178 2898
Size: 178 2720
ApoI r'AATT_y
Cuts at: 0 881 1839 2338 2482 2553 2898
Size: 881 958 499 144 71 345
Fragments arranged by size:
958 881 499 345 144 71
AvaI C'yCGr_G
Cuts at: 0 127 2079 2898
Size: 127 1952 819
Fragments arranged by size:
1952 819 127
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 906 939 2898
Size: 906 33 1959
Fragments arranged by size:
1959 906 33
BamHI G'GATC_C
Cuts at: 0 1667 2898
Size: 1667 1231
BanI G'GyrC_C
Cuts at: 0 960 2371 2386 2898
Size: 960 1411 15 512
Fragments arranged by size:
1411 960 512 15
BanII G_rGCy'C
Cuts at: 0 92 534 1385 1499 2044 2086 2263 2717
Size: 92 442 851 114 545 42 177 454
Cuts at: 2717 2898
Size: 181
Fragments arranged by size:
851 545 454 442 181 177 114 92
42
BbeI G_GCGC'C
Cuts at: 0 964 2898
Size: 964 1934
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 537 2499 2639 2755 2898
Size: 347 190 1962 140 116 143
Fragments arranged by size:
1962 347 190 143 140 116
BciVI GTATCCnnnnn_n'
Cuts at: 0 1541 2898
Size: 1541 1357
BfrBI ATG'CAT
Cuts at: 0 1215 2898
Size: 1215 1683
BglII A'GATC_T
Cuts at: 0 500 2898
Size: 500 2398
BlpI GC'TnA_GC
Cuts at: 0 2243 2898
Size: 2243 655
Bme1580I G_kGCm'C
Cuts at: 0 182 711 2898
Size: 182 529 2187
Fragments arranged by size:
2187 529 182
BmgBI CAC'GTC
Cuts at: 0 1166 2898
Size: 1166 1732
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 519 1196 1399 2574 2898
Size: 519 677 203 1175 324
Fragments arranged by size:
1175 677 519 324 203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2142 2378 2898
Size: 2142 236 520
Fragments arranged by size:
2142 520 236
Bpu10I CC'TnA_GC
Cuts at: 0 22 259 1384 2898
Size: 22 237 1125 1514
Fragments arranged by size:
1514 1125 237 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1447 2898
Size: 1447 1451
BsaHI Gr'CG_yC
Cuts at: 0 961 2674 2898
Size: 961 1713 224
Fragments arranged by size:
1713 961 224
BsaWI w'CCGG_w
Cuts at: 0 1343 1708 2898
Size: 1343 365 1190
Fragments arranged by size:
1343 1190 365
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2080 2110 2898
Size: 2080 30 788
Fragments arranged by size:
2080 788 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 511 2271 2433 2898
Size: 21 490 1760 162 465
Fragments arranged by size:
1760 490 465 162 21
BseYI C'CCAG_C
Cuts at: 0 519 2022 2898
Size: 519 1503 876
Fragments arranged by size:
1503 876 519
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1846 2223 2898
Size: 1846 377 675
Fragments arranged by size:
1846 675 377
BsiEI CG_ry'CG
Cuts at: 0 950 2898
Size: 950 1948
BsiHKAI G_wGCw'C
Cuts at: 0 20 92 182 328 1385 1499 2898
Size: 20 72 90 146 1057 114 1399
Fragments arranged by size:
1399 1057 146 114 90 72 20
BsmI GAATG_Cn'
Cuts at: 0 652 2898
Size: 652 2246
BsmBI CGTCTCn'nnnn_
Cuts at: 0 274 598 2898
Size: 274 324 2300
Fragments arranged by size:
2300 324 274
Bsp1286I G_dGCh'C
Cuts at: 0 20 92 182 328 534 711 1385 1499
Size: 20 72 90 146 206 177 674 114
Cuts at: 1499 2044 2086 2263 2717 2898
Size: 545 42 177 454 181
Fragments arranged by size:
674 545 454 206 181 177 177 146
114 90 72 42 20
BspEI T'CCGG_A
Cuts at: 0 1708 2898
Size: 1708 1190
BspHI T'CATG_A
Cuts at: 0 1595 2898
Size: 1595 1303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 1868 2002 2669 2898
Size: 353 1515 134 667 229
Fragments arranged by size:
1515 667 353 229 134
BsrBI CCG'CTC
Cuts at: 0 541 1419 1431 2898
Size: 541 878 12 1467
Fragments arranged by size:
1467 878 541 12
BsrDI GCAATG_nn'
Cuts at: 0 1223 2898
Size: 1223 1675
BsrFI r'CCGG_y
Cuts at: 0 1343 2898
Size: 1343 1555
BstBI TT'CG_AA
Cuts at: 0 1365 2898
Size: 1365 1533
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1584 1683 2106 2898
Size: 1584 99 423 792
Fragments arranged by size:
1584 792 423 99
BstYI r'GATC_y
Cuts at: 0 500 1049 1667 2898
Size: 500 549 618 1231
Fragments arranged by size:
1231 618 549 500
BstZ17I GTA'TAC
Cuts at: 0 1339 2898
Size: 1339 1559
Bsu36I CC'TnA_GG
Cuts at: 0 2008 2898
Size: 2008 890
BtgI C'CryG_G
Cuts at: 0 711 1583 2105 2898
Size: 711 872 522 793
Fragments arranged by size:
872 793 711 522
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 633 2491 2898
Size: 633 1858 407
Fragments arranged by size:
1858 633 407
BtsI GCAGTG_nn'
Cuts at: 0 378 384 520 1523 1691 2898
Size: 378 6 136 1003 168 1207
Fragments arranged by size:
1207 1003 378 168 136 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 768 803 2898
Size: 768 35 2095
Fragments arranged by size:
2095 768 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 288 2757 2898
Size: 288 2469 141
Fragments arranged by size:
2469 288 141
EaeI y'GGCC_r
Cuts at: 0 2 575 1642 2898
Size: 2 573 1067 1256
Fragments arranged by size:
1256 1067 573 2
EarI CTCTTCn'nnn_
Cuts at: 0 39 563 1373 1895 2422 2444 2570 2838
Size: 39 524 810 522 527 22 126 268
Cuts at: 2838 2898
Size: 60
Fragments arranged by size:
810 527 524 522 268 126 60 39
22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 580 2898
Size: 580 2318
EcoICRI GAG'CTC
Cuts at: 0 90 1383 1497 2898
Size: 90 1293 114 1401
Fragments arranged by size:
1401 1293 114 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 238 359 1058 2142 2182 2378 2819
Size: 36 202 121 699 1084 40 196 441
Cuts at: 2819 2898
Size: 79
Fragments arranged by size:
1084 699 441 202 196 121 79 40
36
EcoO109I rG'GnC_Cy
Cuts at: 0 675 1538 2222 2501 2898
Size: 675 863 684 279 397
Fragments arranged by size:
863 684 675 397 279
EcoRI G'AATT_C
Cuts at: 0 2338 2898
Size: 2338 560
EcoRV GAT'ATC
Cuts at: 0 612 1750 2898
Size: 612 1138 1148
Fragments arranged by size:
1148 1138 612
HaeII r_GCGC'y
Cuts at: 0 964 2401 2898
Size: 964 1437 497
Fragments arranged by size:
1437 964 497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 741 773 826 858 994 1026 1133 1165
Size: 741 32 53 32 136 32 107 32
Cuts at: 1165 1213 1245 1734 1766 1941 1973 2080 2112
Size: 48 32 489 32 175 32 107 32
Cuts at: 2112 2221 2253 2428 2460 2490 2522 2650 2682
Size: 109 32 175 32 30 32 128 32
Cuts at: 2682 2683 2701 2715 2733 2815 2847 2898
Size: 1 18 14 18 82 32 51
Fragments arranged by size:
741 489 175 175 136 128 109 107
107 82 53 51 48 32 32 32
32 32 32 32 32 32 32 32
32 32 30 18 18 14 1
HincII GTy'rAC
Cuts at: 0 206 2898
Size: 206 2692
HindIII A'AGCT_T
Cuts at: 0 1423 2898
Size: 1423 1475
KasI G'GCGC_C
Cuts at: 0 960 2898
Size: 960 1938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1074 2898
Size: 1074 1824
MscI TGG'CCA
Cuts at: 0 4 577 2898
Size: 4 573 2321
Fragments arranged by size:
2321 573 4
MslI CAynn'nnrTG
Cuts at: 0 97 199 869 1577 1681 2104 2708 2898
Size: 97 102 670 708 104 423 604 190
Fragments arranged by size:
708 670 604 423 190 104 102 97
MspA1I CmG'CkG
Cuts at: 0 2070 2345 2522 2898
Size: 2070 275 177 376
Fragments arranged by size:
2070 376 275 177
NarI GG'CG_CC
Cuts at: 0 961 2898
Size: 961 1937
NcoI C'CATG_G
Cuts at: 0 711 1583 2105 2898
Size: 711 872 522 793
Fragments arranged by size:
872 793 711 522
NsiI A_TGCA'T
Cuts at: 0 1217 2898
Size: 1217 1681
NspI r_CATG'y
Cuts at: 0 1393 2898
Size: 1393 1505
PasI CC'CwG_GG
Cuts at: 0 2159 2898
Size: 2159 739
PflMI CCAn_nnn'nTGG
Cuts at: 0 1085 2898
Size: 1085 1813
PpuMI rG'GwC_Cy
Cuts at: 0 1538 2222 2898
Size: 1538 684 676
Fragments arranged by size:
1538 684 676
PspXI vC'TCGA_Gb
Cuts at: 0 127 2079 2898
Size: 127 1952 819
Fragments arranged by size:
1952 819 127
PstI C_TGCA'G
Cuts at: 0 515 1236 1475 1913 2115 2426 2898
Size: 515 721 239 438 202 311 472
Fragments arranged by size:
721 515 472 438 311 239 202
PvuII CAG'CTG
Cuts at: 0 2070 2522 2898
Size: 2070 452 376
Fragments arranged by size:
2070 452 376
SacI G_AGCT'C
Cuts at: 0 92 1385 1499 2898
Size: 92 1293 114 1399
Fragments arranged by size:
1399 1293 114 92
SapI GCTCTTCn'nnn_
Cuts at: 0 1373 2898
Size: 1373 1525
SfcI C'TryA_G
Cuts at: 0 511 1232 1471 1909 2111 2349 2422 2880
Size: 511 721 239 438 202 238 73 458
Cuts at: 2880 2898
Size: 18
Fragments arranged by size:
721 511 458 438 239 238 202 73
18
SfoI GGC'GCC
Cuts at: 0 962 2898
Size: 962 1936
SmlI C'TyrA_G
Cuts at: 0 127 411 1426 2079 2898
Size: 127 284 1015 653 819
Fragments arranged by size:
1015 819 653 284 127
SphI G_CATG'C
Cuts at: 0 1393 2898
Size: 1393 1505
StuI AGG'CCT
Cuts at: 0 417 2418 2898
Size: 417 2001 480
Fragments arranged by size:
2001 480 417
StyI C'CwwG_G
Cuts at: 0 534 711 1583 2105 2898
Size: 534 177 872 522 793
Fragments arranged by size:
872 793 534 522 177
TatI w'GTAC_w
Cuts at: 0 667 1239 2898
Size: 667 572 1659
Fragments arranged by size:
1659 667 572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1680 2898
Size: 1680 1218
XhoI C'TCGA_G
Cuts at: 0 127 2079 2898
Size: 127 1952 819
Fragments arranged by size:
1952 819 127
XmnI GAAnn'nnTTC
Cuts at: 0 1361 2898
Size: 1361 1537
Enzymes that do cut:
AccI AcuI AfeI AflII AgeI AhdI AleI AlwNI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BfrBI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI NsiI NspI PasI PflMI PpuMI PspXI PstI
PvuII SacI SapI SfcI SfoI SmlI SphI StuI
StyI TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AlfI AloI ApaI
AscI AseI AsiSI AvrII BbvCI BcgI BclI BglI
BmrI BmtI BsaI BsaAI BsaBI BsiWI BsrGI BssHII
BssSI BstAPI BstEII ClaI DraI DraIII EagI EcoNI
FalI FseI FspI FspAI HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XmaI ZraI