Restriction map for
pFN21AB0454
(Linear) (Six-base) MAPSORT of: KIBB0454.seq Check: 3134 from: 1 to: 2775
KIBB0454.orf
With 182 enzymes: SgfI *
May 6, 2010 17:03 ..
Acc65I G'GTAC_C
Cuts at: 0 878 2775
Size: 878 1897
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 2775
Size: 1351 1424
AfeI AGC'GCT
Cuts at: 0 811 2775
Size: 811 1964
AflII C'TTAA_G
Cuts at: 0 1402 2775
Size: 1402 1373
AhdI GACnn_n'nnGTC
Cuts at: 0 2638 2775
Size: 2638 137
AleI CACnn'nnGTG
Cuts at: 0 1191 1621 2775
Size: 1191 430 1154
Fragments arranged by size:
1191 1154 430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2618 2775
Size: 2618 157
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1795 1827 2775
Size: 1795 32 948
Fragments arranged by size:
1795 948 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1171 2457 2562 2775
Size: 1171 1286 105 213
Fragments arranged by size:
1286 1171 213 105
ApaI G_GGCC'C
Cuts at: 0 380 593 1061 1266 2775
Size: 380 213 468 205 1509
Fragments arranged by size:
1509 468 380 213 205
ApaLI G'TGCA_C
Cuts at: 0 1426 2356 2775
Size: 1426 930 419
Fragments arranged by size:
1426 930 419
ApoI r'AATT_y
Cuts at: 0 1859 2505 2775
Size: 1859 646 270
Fragments arranged by size:
1859 646 270
AvaI C'yCGr_G
Cuts at: 0 10 318 444 2568 2775
Size: 10 308 126 2124 207
Fragments arranged by size:
2124 308 207 126 10
BamHI G'GATC_C
Cuts at: 0 950 1397 2775
Size: 950 447 1378
Fragments arranged by size:
1378 950 447
BanI G'GyrC_C
Cuts at: 0 745 849 878 1137 2518 2775
Size: 745 104 29 259 1381 257
Fragments arranged by size:
1381 745 259 257 104 29
BanII G_rGCy'C
Cuts at: 0 245 331 380 505 593 823 1061 1266
Size: 245 86 49 125 88 230 238 205
Cuts at: 1266 1514 2054 2204 2278 2383 2569 2775
Size: 248 540 150 74 105 186 206
Fragments arranged by size:
540 248 245 238 230 206 205 186
150 125 105 88 86 74 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 976 1846 2775
Size: 976 870 929
Fragments arranged by size:
976 929 870
BbvCI CC'TCA_GC
Cuts at: 0 2009 2775
Size: 2009 766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 299 333 2775
Size: 299 34 2442
Fragments arranged by size:
2442 299 34
BclI T'GATC_A
Cuts at: 0 905 2775
Size: 905 1870
BglI GCCn_nnn'nGGC
Cuts at: 0 433 1122 2173 2288 2311 2775
Size: 433 689 1051 115 23 464
Fragments arranged by size:
1051 689 464 433 115 23
BglII A'GATC_T
Cuts at: 0 1370 2775
Size: 1370 1405
BlpI GC'TnA_GC
Cuts at: 0 2382 2563 2775
Size: 2382 181 212
Fragments arranged by size:
2382 212 181
Bme1580I G_kGCm'C
Cuts at: 0 380 467 593 716 1061 1266 1430 2360
Size: 380 87 126 123 345 205 164 930
Cuts at: 2360 2775
Size: 415
Fragments arranged by size:
930 415 380 345 205 164 126 123
87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1159 2023 2775
Size: 1159 864 752
Fragments arranged by size:
1159 864 752
BmtI G_CTAG'C
Cuts at: 0 512 2775
Size: 512 2263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2169 2775
Size: 2169 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1331 1516 2775
Size: 1076 255 185 1259
Fragments arranged by size:
1259 1076 255 185
Bpu10I CC'TnA_GC
Cuts at: 0 325 2009 2272 2775
Size: 325 1684 263 503
Fragments arranged by size:
1684 503 325 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 519 1210 2775
Size: 190 329 691 1565
Fragments arranged by size:
1565 691 329 190
BsaAI yAC'GTr
Cuts at: 0 846 2775
Size: 846 1929
BsaBI GATnn'nnATC
Cuts at: 0 340 2775
Size: 340 2435
BsaHI Gr'CG_yC
Cuts at: 0 41 2216 2775
Size: 41 2175 559
Fragments arranged by size:
2175 559 41
BsaWI w'CCGG_w
Cuts at: 0 166 2775
Size: 166 2609
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 850 880 2160 2190 2350 2380
Size: 532 30 288 30 1280 30 160 30
Cuts at: 2380 2775
Size: 395
Fragments arranged by size:
1280 532 395 288 160 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 510 723 1092 2775
Size: 510 213 369 1683
Fragments arranged by size:
1683 510 369 213
BseYI C'CCAG_C
Cuts at: 0 806 874 1709 2034 2126 2238 2278 2286
Size: 806 68 835 325 92 112 40 8
Cuts at: 2286 2775
Size: 489
Fragments arranged by size:
835 806 489 325 112 92 68 40
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 2775
Size: 1261 1514
BsiEI CG_ry'CG
Cuts at: 0 252 1043 2246 2775
Size: 252 791 1203 529
Fragments arranged by size:
1203 791 529 252
BsiHKAI G_wGCw'C
Cuts at: 0 1430 1708 2360 2383 2775
Size: 1430 278 652 23 392
Fragments arranged by size:
1430 652 392 278 23
BsmI GAATG_Cn'
Cuts at: 0 144 759 2607 2775
Size: 144 615 1848 168
Fragments arranged by size:
1848 615 168 144
BsmBI CGTCTCn'nnnn_
Cuts at: 0 386 576 1588 1677 2775
Size: 386 190 1012 89 1098
Fragments arranged by size:
1098 1012 386 190 89
Bsp1286I G_dGCh'C
Cuts at: 0 245 331 380 467 505 593 716 823
Size: 245 86 49 87 38 88 123 107
Cuts at: 823 1061 1266 1430 1514 1708 2054 2204 2278
Size: 238 205 164 84 194 346 150 74
Cuts at: 2278 2360 2383 2569 2775
Size: 82 23 186 206
Fragments arranged by size:
346 245 238 206 205 194 186 164
150 123 107 88 87 86 84 82
74 49 38 23
BspEI T'CCGG_A
Cuts at: 0 166 2775
Size: 166 2609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2673 2775
Size: 2673 102
BsrBI CCG'CTC
Cuts at: 0 15 731 1395 2345 2775
Size: 15 716 664 950 430
Fragments arranged by size:
950 716 664 430 15
BsrFI r'CCGG_y
Cuts at: 0 990 1015 2301 2362 2775
Size: 990 25 1286 61 413
Fragments arranged by size:
1286 990 413 61 25
BssSI C'ACGA_G
Cuts at: 0 52 1723 2161 2607 2775
Size: 52 1671 438 446 168
Fragments arranged by size:
1671 446 438 168 52
BstEII G'GTnAC_C
Cuts at: 0 2673 2775
Size: 2673 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 58 1107 1134 1193 1494 2286 2775
Size: 58 1049 27 59 301 792 489
Fragments arranged by size:
1049 792 489 301 59 58 27
BstYI r'GATC_y
Cuts at: 0 950 1370 1397 1550 1922 2775
Size: 950 420 27 153 372 853
Fragments arranged by size:
950 853 420 372 153 27
BtgI C'CryG_G
Cuts at: 0 861 932 1043 1335 1733 1877 2054 2094
Size: 861 71 111 292 398 144 177 40
Cuts at: 2094 2171 2775
Size: 77 604
Fragments arranged by size:
861 604 398 292 177 144 111 77
71 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 722 2775
Size: 722 2053
BtsI GCAGTG_nn'
Cuts at: 0 377 2775
Size: 377 2398
DraIII CAC_nnn'GTG
Cuts at: 0 1323 2775
Size: 1323 1452
EaeI y'GGCC_r
Cuts at: 0 249 299 425 434 1040 1195 1776 2141
Size: 249 50 126 9 606 155 581 365
Cuts at: 2141 2165 2243 2312 2775
Size: 24 78 69 463
Fragments arranged by size:
606 581 463 365 249 155 126 78
69 50 24 9
EagI C'GGCC_G
Cuts at: 0 249 1040 2243 2775
Size: 249 791 1203 532
Fragments arranged by size:
1203 791 532 249
EarI CTCTTCn'nnn_
Cuts at: 0 103 125 1984 2775
Size: 103 22 1859 791
Fragments arranged by size:
1859 791 103 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1103 2775
Size: 1103 1672
EcoICRI GAG'CTC
Cuts at: 0 2381 2775
Size: 2381 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1331 1351 1516 2775
Size: 1076 255 20 165 1259
Fragments arranged by size:
1259 1076 255 165 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 2775
Size: 679 2096
EcoO109I rG'GnC_Cy
Cuts at: 0 314 376 377 1057 1058 2752 2775
Size: 314 62 1 680 1 1694 23
Fragments arranged by size:
1694 680 314 62 23 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 97 2775
Size: 97 2678
FseI GG_CCGG'CC
Cuts at: 0 1019 2775
Size: 1019 1756
HaeII r_GCGC'y
Cuts at: 0 813 2775
Size: 813 1962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 850 882 1280 1312 1355 1387
Size: 532 32 286 32 398 32 43 32
Cuts at: 1387 1631 1663 2624 2656 2775
Size: 244 32 961 32 119
Fragments arranged by size:
961 532 398 286 244 119 43 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1558 2775
Size: 1558 1217
KpnI G_GTAC'C
Cuts at: 0 882 2775
Size: 882 1893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 2428 2775
Size: 1330 1098 347
Fragments arranged by size:
1330 1098 347
MscI TGG'CCA
Cuts at: 0 2167 2775
Size: 2167 608
MslI CAynn'nnrTG
Cuts at: 0 1165 1191 1621 2775
Size: 1165 26 430 1154
Fragments arranged by size:
1165 1154 430 26
MspA1I CmG'CkG
Cuts at: 0 515 689 1134 1200 1387 1760 2242 2259
Size: 515 174 445 66 187 373 482 17
Cuts at: 2259 2322 2457 2775
Size: 63 135 318
Fragments arranged by size:
515 482 445 373 318 187 174 135
66 63 17
NaeI GCC'GGC
Cuts at: 0 1017 2303 2364 2775
Size: 1017 1286 61 411
Fragments arranged by size:
1286 1017 411 61
NcoI C'CATG_G
Cuts at: 0 1335 1733 2094 2171 2775
Size: 1335 398 361 77 604
Fragments arranged by size:
1335 604 398 361 77
NgoMIV G'CCGG_C
Cuts at: 0 1015 2301 2362 2775
Size: 1015 1286 61 413
Fragments arranged by size:
1286 1015 413 61
NheI G'CTAG_C
Cuts at: 0 508 2775
Size: 508 2267
NspI r_CATG'y
Cuts at: 0 1911 2775
Size: 1911 864
PasI CC'CwG_GG
Cuts at: 0 2370 2775
Size: 2370 405
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 965 1323 2775
Size: 372 593 358 1452
Fragments arranged by size:
1452 593 372 358
PfoI T'CCnGG_A
Cuts at: 0 191 814 1493 2775
Size: 191 623 679 1282
Fragments arranged by size:
1282 679 623 191
PmlI CAC'GTG
Cuts at: 0 846 2775
Size: 846 1929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2556 2588 2775
Size: 2556 32 187
Fragments arranged by size:
2556 187 32
PpuMI rG'GwC_Cy
Cuts at: 0 2752 2775
Size: 2752 23
PshAI GACnn'nnGTC
Cuts at: 0 602 2775
Size: 602 2173
PspOMI G'GGCC_C
Cuts at: 0 376 589 1057 1262 2775
Size: 376 213 468 205 1513
Fragments arranged by size:
1513 468 376 213 205
PstI C_TGCA'G
Cuts at: 0 1159 2086 2775
Size: 1159 927 689
Fragments arranged by size:
1159 927 689
PvuII CAG'CTG
Cuts at: 0 689 1387 1760 2457 2775
Size: 689 698 373 697 318
Fragments arranged by size:
698 697 689 373 318
SacI G_AGCT'C
Cuts at: 0 2383 2775
Size: 2383 392
SapI GCTCTTCn'nnn_
Cuts at: 0 125 2775
Size: 125 2650
SexAI A'CCwGG_T
Cuts at: 0 1421 2775
Size: 1421 1354
SfcI C'TryA_G
Cuts at: 0 1155 2082 2775
Size: 1155 927 693
Fragments arranged by size:
1155 927 693
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 433 2311 2775
Size: 433 1878 464
Fragments arranged by size:
1878 464 433
SgrAI Cr'CCGG_yG
Cuts at: 0 990 2775
Size: 990 1785
SmaI CCC'GGG
Cuts at: 0 2570 2775
Size: 2570 205
SmlI C'TyrA_G
Cuts at: 0 205 498 1189 1402 2775
Size: 205 293 691 213 1373
Fragments arranged by size:
1373 691 293 213 205
StuI AGG'CCT
Cuts at: 0 775 2102 2419 2775
Size: 775 1327 317 356
Fragments arranged by size:
1327 775 356 317
StyI C'CwwG_G
Cuts at: 0 776 1010 1335 1505 1733 2094 2171 2775
Size: 776 234 325 170 228 361 77 604
Fragments arranged by size:
776 604 361 325 234 228 170 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2042 2107 2457 2775
Size: 2042 65 350 318
Fragments arranged by size:
2042 350 318 65
TatI w'GTAC_w
Cuts at: 0 2495 2775
Size: 2495 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2160 2192 2350 2382 2775
Size: 2160 32 158 32 393
Fragments arranged by size:
2160 393 158 32 32
XmaI C'CCGG_G
Cuts at: 0 2568 2775
Size: 2568 207
Enzymes that do cut:
Acc65I AcuI AfeI AflII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI HaeII Hin4I HindIII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SapI SexAI SfcI SfiI SgrAI SmaI SmlI
StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflIII AgeI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BfrBI BmgBI BsaI
BsiWI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FspI
FspAI HincII HpaI KasI MfeI MluI NarI NdeI
NotI NruI NsiI PacI PciI PmeI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI