Restriction map for
pFN21AB0448
(Linear) (Six-base) MAPSORT of: KIBB0448.seq Check: 502 from: 1 to: 2733
KIBB0448.orf
With 182 enzymes: SgfI *
July 3, 2012 16:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1095 1627 2431 2674 2733
Size: 280 815 532 804 243 59
Fragments arranged by size:
815 804 532 280 243 59
AhdI GACnn_n'nnGTC
Cuts at: 0 2539 2733
Size: 2539 194
AleI CACnn'nnGTG
Cuts at: 0 2398 2733
Size: 2398 335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2150 2733
Size: 2150 583
AlwNI CAG_nnn'CTG
Cuts at: 0 798 1212 1591 2338 2733
Size: 798 414 379 747 395
Fragments arranged by size:
798 747 414 395 379
ApaI G_GGCC'C
Cuts at: 0 2197 2263 2299 2733
Size: 2197 66 36 434
Fragments arranged by size:
2197 434 66 36
ApoI r'AATT_y
Cuts at: 0 175 400 745 979 2343 2733
Size: 175 225 345 234 1364 390
Fragments arranged by size:
1364 390 345 234 225 175
BamHI G'GATC_C
Cuts at: 0 1256 2733
Size: 1256 1477
BanI G'GyrC_C
Cuts at: 0 1410 2733
Size: 1410 1323
BanII G_rGCy'C
Cuts at: 0 2197 2263 2299 2733
Size: 2197 66 36 434
Fragments arranged by size:
2197 434 66 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 136 1376 1859 2733
Size: 136 1240 483 874
Fragments arranged by size:
1240 874 483 136
BbvCI CC'TCA_GC
Cuts at: 0 1770 2441 2733
Size: 1770 671 292
Fragments arranged by size:
1770 671 292
BclI T'GATC_A
Cuts at: 0 32 2733
Size: 32 2701
BfrBI ATG'CAT
Cuts at: 0 349 2733
Size: 349 2384
Bme1580I G_kGCm'C
Cuts at: 0 1649 2197 2263 2299 2733
Size: 1649 548 66 36 434
Fragments arranged by size:
1649 548 434 66 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1492 2733
Size: 1492 1241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1820 2733
Size: 1820 913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 188 598 649 2733
Size: 167 21 410 51 2084
Fragments arranged by size:
2084 410 167 51 21
Bpu10I CC'TnA_GC
Cuts at: 0 1770 2441 2733
Size: 1770 671 292
Fragments arranged by size:
1770 671 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1101 2733
Size: 1101 1632
BsaI GGTCTCn'nnnn_
Cuts at: 0 2636 2733
Size: 2636 97
BsaAI yAC'GTr
Cuts at: 0 2529 2733
Size: 2529 204
BsaBI GATnn'nnATC
Cuts at: 0 770 2733
Size: 770 1963
BsaHI Gr'CG_yC
Cuts at: 0 999 2733
Size: 999 1734
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2633 2663 2733
Size: 2633 30 70
Fragments arranged by size:
2633 70 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 694 1807 1825 2715 2733
Size: 694 1113 18 890 18
Fragments arranged by size:
1113 890 694 18 18
BseYI C'CCAG_C
Cuts at: 0 1740 2375 2716 2733
Size: 1740 635 341 17
Fragments arranged by size:
1740 635 341 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1748 2146 2733
Size: 1748 398 587
Fragments arranged by size:
1748 587 398
BsiHKAI G_wGCw'C
Cuts at: 0 2322 2733
Size: 2322 411
BsmI GAATG_Cn'
Cuts at: 0 1531 2733
Size: 1531 1202
BsmBI CGTCTCn'nnnn_
Cuts at: 0 543 903 2605 2733
Size: 543 360 1702 128
Fragments arranged by size:
1702 543 360 128
Bsp1286I G_dGCh'C
Cuts at: 0 1649 2197 2263 2299 2322 2733
Size: 1649 548 66 36 23 411
Fragments arranged by size:
1649 548 411 66 36 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1532 2733
Size: 1532 1201
BsrDI GCAATG_nn'
Cuts at: 0 2294 2733
Size: 2294 439
BstAPI GCAn_nnn'nTGC
Cuts at: 0 642 1591 1621 2733
Size: 642 949 30 1112
Fragments arranged by size:
1112 949 642 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1923 2400 2733
Size: 1923 477 333
Fragments arranged by size:
1923 477 333
BstYI r'GATC_y
Cuts at: 0 1256 2733
Size: 1256 1477
Bsu36I CC'TnA_GG
Cuts at: 0 655 1805 2202 2733
Size: 655 1150 397 531
Fragments arranged by size:
1150 655 531 397
BtgI C'CryG_G
Cuts at: 0 238 1221 2733
Size: 238 983 1512
Fragments arranged by size:
1512 983 238
ClaI AT'CG_AT
Cuts at: 0 111 2733
Size: 111 2622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1887 1922 2733
Size: 1887 35 811
Fragments arranged by size:
1887 811 35
DraI TTT'AAA
Cuts at: 0 984 2733
Size: 984 1749
DraIII CAC_nnn'GTG
Cuts at: 0 2497 2608 2733
Size: 2497 111 125
Fragments arranged by size:
2497 125 111
EaeI y'GGCC_r
Cuts at: 0 1182 1626 1968 2733
Size: 1182 444 342 765
Fragments arranged by size:
1182 765 444 342
EarI CTCTTCn'nnn_
Cuts at: 0 689 831 1058 1982 2650 2655 2733
Size: 689 142 227 924 668 5 78
Fragments arranged by size:
924 689 668 227 142 78 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 188 280 598 649 1095 1627 2431
Size: 167 21 92 318 51 446 532 804
Cuts at: 2431 2674 2733
Size: 243 59
Fragments arranged by size:
804 532 446 318 243 167 92 59
51 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1789 2733
Size: 1789 944
EcoO109I rG'GnC_Cy
Cuts at: 0 2260 2335 2733
Size: 2260 75 398
Fragments arranged by size:
2260 398 75
HaeII r_GCGC'y
Cuts at: 0 1785 2729 2733
Size: 1785 944 4
Fragments arranged by size:
1785 944 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 808 840 903 935 1099 1131 1714 1744
Size: 808 32 63 32 164 32 583 30
Cuts at: 1744 1746 1776 1894 1926 2525 2557 2633 2665
Size: 2 30 118 32 599 32 76 32
Cuts at: 2665 2696 2728 2733
Size: 31 32 5
Fragments arranged by size:
808 599 583 164 118 76 63 32
32 32 32 32 32 32 31 30
30 5 2
HincII GTy'rAC
Cuts at: 0 219 491 1464 2733
Size: 219 272 973 1269
Fragments arranged by size:
1269 973 272 219
HindIII A'AGCT_T
Cuts at: 0 211 2733
Size: 211 2522
MfeI C'AATT_G
Cuts at: 0 152 2733
Size: 152 2581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 738 2529 2619 2733
Size: 348 390 1791 90 114
Fragments arranged by size:
1791 390 348 114 90
MscI TGG'CCA
Cuts at: 0 1184 1628 1970 2733
Size: 1184 444 342 763
Fragments arranged by size:
1184 763 444 342
MslI CAynn'nnrTG
Cuts at: 0 1960 1964 2398 2733
Size: 1960 4 434 335
Fragments arranged by size:
1960 434 335 4
MspA1I CmG'CkG
Cuts at: 0 1348 1863 2211 2398 2720 2733
Size: 1348 515 348 187 322 13
Fragments arranged by size:
1348 515 348 322 187 13
NcoI C'CATG_G
Cuts at: 0 238 1221 2733
Size: 238 983 1512
Fragments arranged by size:
1512 983 238
NdeI CA'TA_TG
Cuts at: 0 1179 2733
Size: 1179 1554
NsiI A_TGCA'T
Cuts at: 0 351 2733
Size: 351 2382
NspI r_CATG'y
Cuts at: 0 1057 2733
Size: 1057 1676
PpuMI rG'GwC_Cy
Cuts at: 0 2335 2733
Size: 2335 398
PshAI GACnn'nnGTC
Cuts at: 0 1695 2733
Size: 1695 1038
PsiI TTA'TAA
Cuts at: 0 743 2733
Size: 743 1990
PspOMI G'GGCC_C
Cuts at: 0 2193 2259 2295 2733
Size: 2193 66 36 438
Fragments arranged by size:
2193 438 66 36
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 779 811 2733
Size: 779 32 1922
Fragments arranged by size:
1922 779 32
PstI C_TGCA'G
Cuts at: 0 1529 1587 1685 1986 2139 2436 2733
Size: 1529 58 98 301 153 297 297
Fragments arranged by size:
1529 301 297 297 153 98 58
PvuII CAG'CTG
Cuts at: 0 1348 2211 2398 2733
Size: 1348 863 187 335
Fragments arranged by size:
1348 863 335 187
SapI GCTCTTCn'nnn_
Cuts at: 0 689 1058 2733
Size: 689 369 1675
Fragments arranged by size:
1675 689 369
SbfI CC_TGCA'GG
Cuts at: 0 1529 2139 2733
Size: 1529 610 594
Fragments arranged by size:
1529 610 594
SfcI C'TryA_G
Cuts at: 0 364 1353 1525 1583 1681 1982 2135 2432
Size: 364 989 172 58 98 301 153 297
Cuts at: 2432 2733
Size: 301
Fragments arranged by size:
989 364 301 301 297 172 153 98
58
SmlI C'TyrA_G
Cuts at: 0 1116 2733
Size: 1116 1617
SspI AAT'ATT
Cuts at: 0 1474 2733
Size: 1474 1259
StyI C'CwwG_G
Cuts at: 0 238 1221 2361 2637 2733
Size: 238 983 1140 276 96
Fragments arranged by size:
1140 983 276 238 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 1511 2733
Size: 261 1250 1222
Fragments arranged by size:
1250 1222 261
TatI w'GTAC_w
Cuts at: 0 2157 2733
Size: 2157 576
Tth111I GACn'n_nGTC
Cuts at: 0 2647 2733
Size: 2647 86
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1088 1404 2733
Size: 1088 316 1329
Fragments arranged by size:
1329 1088 316
XmnI GAAnn'nnTTC
Cuts at: 0 2043 2733
Size: 2043 690
Enzymes that do cut:
AcuI AhdI AleI AlfI AlwNI ApaI ApoI BamHI
BanI BanII BbsI BbvCI BclI BfrBI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI ClaI
CspCI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuII SapI SbfI SfcI SmlI
SspI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BglI BglII BlpI BmgBI
BmtI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PspXI PvuI RsrII SacI
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI XbaI XhoI XmaI ZraI