Restriction map for
pFN21AB0433
(Linear) (Six-base) MAPSORT of: KIBB0433.seq Check: 1858 from: 1 to: 1368
KIBB0433.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AccI GT'mk_AC
Cuts at: 0 21 1368
Size: 21 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1056 1368
Size: 34 1022 312
Fragments arranged by size:
1022 312 34
AfeI AGC'GCT
Cuts at: 0 234 1368
Size: 234 1134
AflII C'TTAA_G
Cuts at: 0 801 940 1368
Size: 801 139 428
Fragments arranged by size:
801 428 139
AleI CACnn'nnGTG
Cuts at: 0 15 1368
Size: 15 1353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 310 1368
Size: 310 1058
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1087 1119 1368
Size: 1087 32 249
Fragments arranged by size:
1087 249 32
AlwNI CAG_nnn'CTG
Cuts at: 0 198 586 709 1295 1368
Size: 198 388 123 586 73
Fragments arranged by size:
586 388 198 123 73
ApaI G_GGCC'C
Cuts at: 0 242 1368
Size: 242 1126
ApoI r'AATT_y
Cuts at: 0 346 1368
Size: 346 1022
AvaI C'yCGr_G
Cuts at: 0 71 1124 1368
Size: 71 1053 244
Fragments arranged by size:
1053 244 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1039 1072 1368
Size: 1039 33 296
Fragments arranged by size:
1039 296 33
BamHI G'GATC_C
Cuts at: 0 1353 1368
Size: 1353 15
BanI G'GyrC_C
Cuts at: 0 41 208 1368
Size: 41 167 1160
Fragments arranged by size:
1160 167 41
BanII G_rGCy'C
Cuts at: 0 242 537 1368
Size: 242 295 831
Fragments arranged by size:
831 295 242
BglI GCCn_nnn'nGGC
Cuts at: 0 1259 1368
Size: 1259 109
BglII A'GATC_T
Cuts at: 0 1302 1368
Size: 1302 66
Bme1580I G_kGCm'C
Cuts at: 0 46 213 242 908 1226 1284 1368
Size: 46 167 29 666 318 58 84
Fragments arranged by size:
666 318 167 84 58 46 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 1014 1368
Size: 207 807 354
Fragments arranged by size:
807 354 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 495 1118 1368
Size: 303 192 623 250
Fragments arranged by size:
623 303 250 192
Bpu10I CC'TnA_GC
Cuts at: 0 579 1349 1368
Size: 579 770 19
Fragments arranged by size:
770 579 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1368
Size: 358 1010
BsaI GGTCTCn'nnnn_
Cuts at: 0 1136 1368
Size: 1136 232
BsaAI yAC'GTr
Cuts at: 0 180 415 634 1368
Size: 180 235 219 734
Fragments arranged by size:
734 235 219 180
BsaBI GATnn'nnATC
Cuts at: 0 1271 1368
Size: 1271 97
BsaWI w'CCGG_w
Cuts at: 0 121 1368
Size: 121 1247
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 394 1153 1368
Size: 325 69 759 215
Fragments arranged by size:
759 325 215 69
BseYI C'CCAG_C
Cuts at: 0 235 814 901 1368
Size: 235 579 87 467
Fragments arranged by size:
579 467 235 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1368
Size: 790 578
BsiHKAI G_wGCw'C
Cuts at: 0 1244 1368
Size: 1244 124
BsmI GAATG_Cn'
Cuts at: 0 517 1368
Size: 517 851
Bsp1286I G_dGCh'C
Cuts at: 0 46 213 242 537 908 1226 1244 1284
Size: 46 167 29 295 371 318 18 40
Cuts at: 1284 1368
Size: 84
Fragments arranged by size:
371 318 295 167 84 46 40 29
18
BsrFI r'CCGG_y
Cuts at: 0 1253 1368
Size: 1253 115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1239 1368
Size: 1239 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 157 933 1368
Size: 157 776 435
Fragments arranged by size:
776 435 157
BstYI r'GATC_y
Cuts at: 0 527 930 1302 1353 1368
Size: 527 403 372 51 15
Fragments arranged by size:
527 403 372 51 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 130 1132 1368
Size: 130 1002 236
Fragments arranged by size:
1002 236 130
BtsI GCAGTG_nn'
Cuts at: 0 404 1368
Size: 404 964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 545 580 1368
Size: 545 35 788
Fragments arranged by size:
788 545 35
EarI CTCTTCn'nnn_
Cuts at: 0 130 1368
Size: 130 1238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 303 495 1056 1118 1368
Size: 34 269 192 561 62 250
Fragments arranged by size:
561 269 250 192 62 34
HaeII r_GCGC'y
Cuts at: 0 79 236 1368
Size: 79 157 1132
Fragments arranged by size:
1132 157 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 129 135 161 261 293 519 551
Size: 103 26 6 26 100 32 226 32
Cuts at: 551 562 594 1107 1139 1368
Size: 11 32 513 32 229
Fragments arranged by size:
513 229 226 103 100 32 32 32
32 26 26 11 6
HincII GTy'rAC
Cuts at: 0 1025 1368
Size: 1025 343
MslI CAynn'nnrTG
Cuts at: 0 15 1368
Size: 15 1353
MspA1I CmG'CkG
Cuts at: 0 661 1076 1368
Size: 661 415 292
Fragments arranged by size:
661 415 292
NaeI GCC'GGC
Cuts at: 0 1255 1368
Size: 1255 113
NgoMIV G'CCGG_C
Cuts at: 0 1253 1368
Size: 1253 115
NspI r_CATG'y
Cuts at: 0 1230 1237 1368
Size: 1230 7 131
Fragments arranged by size:
1230 131 7
PfoI T'CCnGG_A
Cuts at: 0 350 1368
Size: 350 1018
PmlI CAC'GTG
Cuts at: 0 180 1368
Size: 180 1188
PspOMI G'GGCC_C
Cuts at: 0 238 1368
Size: 238 1130
PstI C_TGCA'G
Cuts at: 0 134 1368
Size: 134 1234
PvuII CAG'CTG
Cuts at: 0 661 1076 1368
Size: 661 415 292
Fragments arranged by size:
661 415 292
SexAI A'CCwGG_T
Cuts at: 0 975 1368
Size: 975 393
SfcI C'TryA_G
Cuts at: 0 130 299 1038 1368
Size: 130 169 739 330
Fragments arranged by size:
739 330 169 130
SmaI CCC'GGG
Cuts at: 0 73 1368
Size: 73 1295
SmlI C'TyrA_G
Cuts at: 0 337 801 940 1368
Size: 337 464 139 428
Fragments arranged by size:
464 428 337 139
SnaBI TAC'GTA
Cuts at: 0 415 1368
Size: 415 953
SphI G_CATG'C
Cuts at: 0 1237 1368
Size: 1237 131
SrfI GCCC'GGGC
Cuts at: 0 73 1368
Size: 73 1295
StuI AGG'CCT
Cuts at: 0 141 1368
Size: 141 1227
StyI C'CwwG_G
Cuts at: 0 51 764 1368
Size: 51 713 604
Fragments arranged by size:
713 604 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 777 1368
Size: 777 591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 672 1368
Size: 672 696
XmaI C'CCGG_G
Cuts at: 0 71 1368
Size: 71 1297
XmnI GAAnn'nnTTC
Cuts at: 0 346 1368
Size: 346 1022
Enzymes that do cut:
AccI AcuI AfeI AflII AleI AlfI AloI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BglI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrFI BstAPI BstXI BstYI BtgZI BtsI CspCI EarI
Eco57MI HaeII Hin4I HincII MslI MspA1I NaeI NgoMIV
NspI PfoI PmlI PspOMI PstI PvuII SexAI SfcI
SmaI SmlI SnaBI SphI SrfI StuI StyI TaqII
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI ApaLI
AscI AseI AsiSI AvrII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaHI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgI ClaI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SpeI SspI SwaI TatI TstI Tth111I XbaI XhoI
ZraI