Restriction map for
pFN21AB0431
(Linear) (Six-base) MAPSORT of: KIBB0431.seq Check: 6278 from: 1 to: 1428
KIBB0431.orf
With 182 enzymes: SgfI *
January 27, 2011 13:24 ..
AatII G_ACGT'C
Cuts at: 0 1009 1428
Size: 1009 419
AclI AA'CG_TT
Cuts at: 0 741 1428
Size: 741 687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1428
Size: 1088 340
AflII C'TTAA_G
Cuts at: 0 850 1428
Size: 850 578
ApaI G_GGCC'C
Cuts at: 0 58 1428
Size: 58 1370
ApoI r'AATT_y
Cuts at: 0 689 1428
Size: 689 739
AseI AT'TA_AT
Cuts at: 0 347 1428
Size: 347 1081
AvaI C'yCGr_G
Cuts at: 0 57 1428
Size: 57 1371
BanII G_rGCy'C
Cuts at: 0 58 626 998 1113 1218 1338 1428
Size: 58 568 372 115 105 120 90
Fragments arranged by size:
568 372 120 115 105 90 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 574 619 1428
Size: 574 45 809
Fragments arranged by size:
809 574 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 673 707 1373 1407 1428
Size: 673 34 666 34 21
Fragments arranged by size:
673 666 34 34 21
BglI GCCn_nnn'nGGC
Cuts at: 0 1128 1428
Size: 1128 300
BglII A'GATC_T
Cuts at: 0 1048 1428
Size: 1048 380
Bme1580I G_kGCm'C
Cuts at: 0 58 948 1428
Size: 58 890 480
Fragments arranged by size:
890 480 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 1249 1428
Size: 1249 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 941 1009 1428
Size: 271 670 68 419
Fragments arranged by size:
670 419 271 68
Bpu10I CC'TnA_GC
Cuts at: 0 199 1428
Size: 199 1229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1037 1428
Size: 318 719 391
Fragments arranged by size:
719 391 318
BsaHI Gr'CG_yC
Cuts at: 0 130 1006 1428
Size: 130 876 422
Fragments arranged by size:
876 422 130
BsaWI w'CCGG_w
Cuts at: 0 148 1428
Size: 148 1280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 1428
Size: 61 30 1337
Fragments arranged by size:
1337 61 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 268 732 735 917 922 1006 1428
Size: 196 72 464 3 182 5 84 422
Fragments arranged by size:
464 422 196 182 84 72 5 3
BseYI C'CCAG_C
Cuts at: 0 594 1428
Size: 594 834
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 974 1428
Size: 112 862 454
Fragments arranged by size:
862 454 112
BsiEI CG_ry'CG
Cuts at: 0 41 1428
Size: 41 1387
BsiHKAI G_wGCw'C
Cuts at: 0 626 1034 1113 1338 1428
Size: 626 408 79 225 90
Fragments arranged by size:
626 408 225 90 79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 121 997 1428
Size: 121 876 431
Fragments arranged by size:
876 431 121
Bsp1286I G_dGCh'C
Cuts at: 0 58 626 948 998 1034 1113 1218 1338
Size: 58 568 322 50 36 79 105 120
Cuts at: 1338 1428
Size: 90
Fragments arranged by size:
568 322 120 105 90 79 58 50
36
BsrBI CCG'CTC
Cuts at: 0 62 1428
Size: 62 1366
BsrDI GCAATG_nn'
Cuts at: 0 626 1428
Size: 626 802
BsrFI r'CCGG_y
Cuts at: 0 1304 1428
Size: 1304 124
BssSI C'ACGA_G
Cuts at: 0 1117 1428
Size: 1117 311
BstXI CCAn_nnnn'nTGG
Cuts at: 0 975 1428
Size: 975 453
BstYI r'GATC_y
Cuts at: 0 1048 1373 1428
Size: 1048 325 55
Fragments arranged by size:
1048 325 55
BtgI C'CryG_G
Cuts at: 0 974 1229 1428
Size: 974 255 199
Fragments arranged by size:
974 255 199
BtsI GCAGTG_nn'
Cuts at: 0 443 1026 1428
Size: 443 583 402
Fragments arranged by size:
583 443 402
EaeI y'GGCC_r
Cuts at: 0 38 1176 1306 1428
Size: 38 1138 130 122
Fragments arranged by size:
1138 130 122 38
EagI C'GGCC_G
Cuts at: 0 38 1428
Size: 38 1390
EarI CTCTTCn'nnn_
Cuts at: 0 181 557 692 746 765 1239 1405 1412
Size: 181 376 135 54 19 474 166 7
Cuts at: 1412 1428
Size: 16
Fragments arranged by size:
474 376 181 166 135 54 19 16
7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 205 1376 1428
Size: 205 1171 52
Fragments arranged by size:
1171 205 52
EcoICRI GAG'CTC
Cuts at: 0 624 1111 1336 1428
Size: 624 487 225 92
Fragments arranged by size:
624 487 225 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 941 1009 1088 1428
Size: 271 670 68 79 340
Fragments arranged by size:
670 340 271 79 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 238 1428
Size: 202 36 1190
Fragments arranged by size:
1190 202 36
EcoO109I rG'GnC_Cy
Cuts at: 0 54 1171 1428
Size: 54 1117 257
Fragments arranged by size:
1117 257 54
EcoRI G'AATT_C
Cuts at: 0 689 1428
Size: 689 739
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 303 335 1428
Size: 61 32 210 32 1093
Fragments arranged by size:
1093 210 61 32 32
HindIII A'AGCT_T
Cuts at: 0 844 1428
Size: 844 584
MscI TGG'CCA
Cuts at: 0 1178 1428
Size: 1178 250
MslI CAynn'nnrTG
Cuts at: 0 1037 1088 1428
Size: 1037 51 340
Fragments arranged by size:
1037 340 51
MspA1I CmG'CkG
Cuts at: 0 249 261 270 1016 1211 1320 1428
Size: 249 12 9 746 195 109 108
Fragments arranged by size:
746 249 195 109 108 12 9
NcoI C'CATG_G
Cuts at: 0 974 1229 1428
Size: 974 255 199
Fragments arranged by size:
974 255 199
NruI TCG'CGA
Cuts at: 0 179 1428
Size: 179 1249
PfoI T'CCnGG_A
Cuts at: 0 1329 1428
Size: 1329 99
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 662 1428
Size: 630 32 766
Fragments arranged by size:
766 630 32
PpuMI rG'GwC_Cy
Cuts at: 0 1171 1428
Size: 1171 257
PshAI GACnn'nnGTC
Cuts at: 0 311 1265 1428
Size: 311 954 163
Fragments arranged by size:
954 311 163
PspOMI G'GGCC_C
Cuts at: 0 54 1428
Size: 54 1374
PstI C_TGCA'G
Cuts at: 0 438 1428
Size: 438 990
PvuII CAG'CTG
Cuts at: 0 249 261 1320 1428
Size: 249 12 1059 108
Fragments arranged by size:
1059 249 108 12
SacI G_AGCT'C
Cuts at: 0 626 1113 1338 1428
Size: 626 487 225 90
Fragments arranged by size:
626 487 225 90
SapI GCTCTTCn'nnn_
Cuts at: 0 1239 1428
Size: 1239 189
SfcI C'TryA_G
Cuts at: 0 434 1428
Size: 434 994
SmlI C'TyrA_G
Cuts at: 0 333 850 1052 1428
Size: 333 517 202 376
Fragments arranged by size:
517 376 333 202
StuI AGG'CCT
Cuts at: 0 29 422 1428
Size: 29 393 1006
Fragments arranged by size:
1006 393 29
StyI C'CwwG_G
Cuts at: 0 239 974 1229 1428
Size: 239 735 255 199
Fragments arranged by size:
735 255 239 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1290 1428
Size: 1290 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 648 1236 1428
Size: 246 402 588 192
Fragments arranged by size:
588 402 246 192
ZraI GAC'GTC
Cuts at: 0 1007 1428
Size: 1007 421
Enzymes that do cut:
AatII AclI AcuI AflII ApaI ApoI AseI AvaI
BanII BbsI BcgI BglI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssSI
BstXI BstYI BtgI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII MscI
MslI MspA1I NcoI NruI PfoI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SapI SfcI SmlI StuI
StyI TaqII XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsiWI BsmI
BspEI BspHI BspMI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI