Restriction map for
pFN21AB0424
(Linear) (Six-base) MAPSORT of: KIBB0424.seq Check: 3723 from: 1 to: 2517
KIBB0424.orf
With 182 enzymes: SgfI *
March 22, 2012 15:30 ..
AclI AA'CG_TT
Cuts at: 0 133 2517
Size: 133 2384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 167 329 607 1341 1824 2371 2517
Size: 141 26 162 278 734 483 547 146
Fragments arranged by size:
734 547 483 278 162 146 141 26
AfeI AGC'GCT
Cuts at: 0 154 2517
Size: 154 2363
AflII C'TTAA_G
Cuts at: 0 2493 2517
Size: 2493 24
AflIII A'CryG_T
Cuts at: 0 1764 2517
Size: 1764 753
AleI CACnn'nnGTG
Cuts at: 0 1262 1799 2517
Size: 1262 537 718
Fragments arranged by size:
1262 718 537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 434 2517
Size: 127 307 2083
Fragments arranged by size:
2083 307 127
AlwNI CAG_nnn'CTG
Cuts at: 0 267 294 603 2517
Size: 267 27 309 1914
Fragments arranged by size:
1914 309 267 27
ApaLI G'TGCA_C
Cuts at: 0 1159 1682 1768 1876 2517
Size: 1159 523 86 108 641
Fragments arranged by size:
1159 641 523 108 86
ApoI r'AATT_y
Cuts at: 0 1057 1424 1544 2424 2517
Size: 1057 367 120 880 93
Fragments arranged by size:
1057 880 367 120 93
AvaI C'yCGr_G
Cuts at: 0 17 191 241 1967 2517
Size: 17 174 50 1726 550
Fragments arranged by size:
1726 550 174 50 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 907 940 2517
Size: 907 33 1577
Fragments arranged by size:
1577 907 33
BamHI G'GATC_C
Cuts at: 0 876 2517
Size: 876 1641
BanI G'GyrC_C
Cuts at: 0 843 2517
Size: 843 1674
BanII G_rGCy'C
Cuts at: 0 800 2517
Size: 800 1717
BbeI G_GCGC'C
Cuts at: 0 847 2517
Size: 847 1670
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 104 2123 2517
Size: 74 30 2019 394
Fragments arranged by size:
2019 394 74 30
BbvCI CC'TCA_GC
Cuts at: 0 235 2517
Size: 235 2282
BciVI GTATCCnnnnn_n'
Cuts at: 0 684 2517
Size: 684 1833
Bme1580I G_kGCm'C
Cuts at: 0 1037 1163 1686 1772 1880 2517
Size: 1037 126 523 86 108 637
Fragments arranged by size:
1037 637 523 126 108 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 635 846 1724 2517
Size: 635 211 878 793
Fragments arranged by size:
878 793 635 211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 887 2517
Size: 887 1630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 304 479 562 706 1127 1652 2286
Size: 289 15 175 83 144 421 525 634
Cuts at: 2286 2454 2517
Size: 168 63
Fragments arranged by size:
634 525 421 289 175 168 144 83
63 15
Bpu10I CC'TnA_GC
Cuts at: 0 235 512 1007 2517
Size: 235 277 495 1510
Fragments arranged by size:
1510 495 277 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1469 2517
Size: 415 1054 1048
Fragments arranged by size:
1054 1048 415
BsaI GGTCTCn'nnnn_
Cuts at: 0 1632 1884 2513 2517
Size: 1632 252 629 4
Fragments arranged by size:
1632 629 252 4
BsaHI Gr'CG_yC
Cuts at: 0 844 2517
Size: 844 1673
BseYI C'CCAG_C
Cuts at: 0 42 506 1169 2517
Size: 42 464 663 1348
Fragments arranged by size:
1348 663 464 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 232 487 2517
Size: 87 145 255 2030
Fragments arranged by size:
2030 255 145 87
BsiEI CG_ry'CG
Cuts at: 0 1320 2517
Size: 1320 1197
BsiHKAI G_wGCw'C
Cuts at: 0 221 800 1163 1686 1772 1880 2045 2517
Size: 221 579 363 523 86 108 165 472
Fragments arranged by size:
579 523 472 363 221 165 108 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 541 2517
Size: 541 1976
Bsp1286I G_dGCh'C
Cuts at: 0 221 800 1037 1163 1686 1772 1880 2045
Size: 221 579 237 126 523 86 108 165
Cuts at: 2045 2517
Size: 472
Fragments arranged by size:
579 523 472 237 221 165 126 108
86
BspHI T'CATG_A
Cuts at: 0 2325 2517
Size: 2325 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 918 2517
Size: 918 1599
BsrDI GCAATG_nn'
Cuts at: 0 1302 2517
Size: 1302 1215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1945 2517
Size: 1945 572
BstYI r'GATC_y
Cuts at: 0 263 876 978 2478 2517
Size: 263 613 102 1500 39
Fragments arranged by size:
1500 613 263 102 39
Bsu36I CC'TnA_GG
Cuts at: 0 872 2517
Size: 872 1645
BtgI C'CryG_G
Cuts at: 0 497 767 2517
Size: 497 270 1750
Fragments arranged by size:
1750 497 270
BtsI GCAGTG_nn'
Cuts at: 0 470 1386 2517
Size: 470 916 1131
Fragments arranged by size:
1131 916 470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1284 1319 2517
Size: 1284 35 1198
Fragments arranged by size:
1284 1198 35
DraI TTT'AAA
Cuts at: 0 2451 2517
Size: 2451 66
DraIII CAC_nnn'GTG
Cuts at: 0 840 2517
Size: 840 1677
EaeI y'GGCC_r
Cuts at: 0 365 2262 2517
Size: 365 1897 255
Fragments arranged by size:
1897 365 255
EarI CTCTTCn'nnn_
Cuts at: 0 305 350 647 1135 2517
Size: 305 45 297 488 1382
Fragments arranged by size:
1382 488 305 297 45
EcoICRI GAG'CTC
Cuts at: 0 798 2517
Size: 798 1719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 167 289 304 329 479 562 607
Size: 141 26 122 15 25 150 83 45
Cuts at: 607 706 1127 1341 1652 1824 2286 2371 2454
Size: 99 421 214 311 172 462 85 83
Cuts at: 2454 2517
Size: 63
Fragments arranged by size:
462 421 311 214 172 150 141 122
99 85 83 83 63 45 26 25
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 327 2517
Size: 327 2190
EcoO109I rG'GnC_Cy
Cuts at: 0 187 629 1283 1850 2517
Size: 187 442 654 567 667
Fragments arranged by size:
667 654 567 442 187
EcoRI G'AATT_C
Cuts at: 0 1544 2517
Size: 1544 973
EcoRV GAT'ATC
Cuts at: 0 2160 2517
Size: 2160 357
HaeII r_GCGC'y
Cuts at: 0 156 523 847 2517
Size: 156 367 324 1670
Fragments arranged by size:
1670 367 324 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 321 353 814 846 1134 1166 1671 1703
Size: 321 32 461 32 288 32 505 32
Cuts at: 1703 2463 2495 2517
Size: 760 32 22
Fragments arranged by size:
760 505 461 321 288 32 32 32
32 32 22
HincII GTy'rAC
Cuts at: 0 933 2517
Size: 933 1584
HindIII A'AGCT_T
Cuts at: 0 1668 2517
Size: 1668 849
KasI G'GCGC_C
Cuts at: 0 843 2517
Size: 843 1674
MluI A'CGCG_T
Cuts at: 0 1764 2517
Size: 1764 753
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2077 2517
Size: 2077 440
MscI TGG'CCA
Cuts at: 0 2264 2517
Size: 2264 253
MslI CAynn'nnrTG
Cuts at: 0 109 1262 1647 1731 1799 2330 2387 2517
Size: 109 1153 385 84 68 531 57 130
Fragments arranged by size:
1153 531 385 130 109 84 68 57
MspA1I CmG'CkG
Cuts at: 0 46 1155 1274 2432 2517
Size: 46 1109 119 1158 85
Fragments arranged by size:
1158 1109 119 85 46
NarI GG'CG_CC
Cuts at: 0 844 2517
Size: 844 1673
NcoI C'CATG_G
Cuts at: 0 767 2517
Size: 767 1750
NdeI CA'TA_TG
Cuts at: 0 1608 2517
Size: 1608 909
PasI CC'CwG_GG
Cuts at: 0 323 2517
Size: 323 2194
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 2517
Size: 639 1878
PfoI T'CCnGG_A
Cuts at: 0 266 539 683 2517
Size: 266 273 144 1834
Fragments arranged by size:
1834 273 266 144
PpuMI rG'GwC_Cy
Cuts at: 0 187 629 1850 2517
Size: 187 442 1221 667
Fragments arranged by size:
1221 667 442 187
PstI C_TGCA'G
Cuts at: 0 481 2517
Size: 481 2036
PvuII CAG'CTG
Cuts at: 0 46 2432 2517
Size: 46 2386 85
Fragments arranged by size:
2386 85 46
SacI G_AGCT'C
Cuts at: 0 800 2517
Size: 800 1717
SanDI GG'GwC_CC
Cuts at: 0 629 2517
Size: 629 1888
SapI GCTCTTCn'nnn_
Cuts at: 0 1135 2517
Size: 1135 1382
SexAI A'CCwGG_T
Cuts at: 0 1247 2517
Size: 1247 1270
SfcI C'TryA_G
Cuts at: 0 477 1593 2517
Size: 477 1116 924
Fragments arranged by size:
1116 924 477
SfoI GGC'GCC
Cuts at: 0 845 2517
Size: 845 1672
SmaI CCC'GGG
Cuts at: 0 193 2517
Size: 193 2324
SmlI C'TyrA_G
Cuts at: 0 394 1484 2493 2517
Size: 394 1090 1009 24
Fragments arranged by size:
1090 1009 394 24
SspI AAT'ATT
Cuts at: 0 1435 2517
Size: 1435 1082
StyI C'CwwG_G
Cuts at: 0 767 2517
Size: 767 1750
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 315 520 828 1183 1306 2517
Size: 25 290 205 308 355 123 1211
Fragments arranged by size:
1211 355 308 290 205 123 25
TatI w'GTAC_w
Cuts at: 0 119 2517
Size: 119 2398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1606 1638 2517
Size: 1606 32 879
Fragments arranged by size:
1606 879 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 789 2517
Size: 789 1728
XmaI C'CCGG_G
Cuts at: 0 191 2517
Size: 191 2326
XmnI GAAnn'nnTTC
Cuts at: 0 1312 1479 1669 1726 1837 2517
Size: 1312 167 190 57 111 680
Fragments arranged by size:
1312 680 190 167 111 57
Enzymes that do cut:
AclI AcuI AfeI AflII AflIII AleI AlfI AlwNI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrDI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII
KasI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI PasI PflMI PfoI PpuMI PstI PvuII SacI
SanDI SapI SexAI SfcI SfoI SmaI SmlI SspI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI
BseRI BsiWI BsmI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DrdI
EagI EciI FalI FseI FspI FspAI HpaI KpnI
MfeI NaeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XhoI ZraI