Restriction map for
pFN21AB0412
(Linear) (Six-base) MAPSORT of: KIBB0412.seq Check: 7766 from: 1 to: 2472
KIBB0412.orf
With 182 enzymes: SgfI *
July 3, 2012 16:00 ..
AccI GT'mk_AC
Cuts at: 0 612 2472
Size: 612 1860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 2214 2472
Size: 1084 1130 258
Fragments arranged by size:
1130 1084 258
AflII C'TTAA_G
Cuts at: 0 384 2472
Size: 384 2088
AflIII A'CryG_T
Cuts at: 0 1760 2472
Size: 1760 712
AhdI GACnn_n'nnGTC
Cuts at: 0 192 1513 2472
Size: 192 1321 959
Fragments arranged by size:
1321 959 192
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1901 1933 2472
Size: 1901 32 539
Fragments arranged by size:
1901 539 32
AlwNI CAG_nnn'CTG
Cuts at: 0 717 906 1855 2472
Size: 717 189 949 617
Fragments arranged by size:
949 717 617 189
ApoI r'AATT_y
Cuts at: 0 742 798 917 1424 1451 1641 2472
Size: 742 56 119 507 27 190 831
Fragments arranged by size:
831 742 507 190 119 56 27
AvaI C'yCGr_G
Cuts at: 0 21 100 2472
Size: 21 79 2372
Fragments arranged by size:
2372 79 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 372 405 2472
Size: 372 33 2067
Fragments arranged by size:
2067 372 33
BamHI G'GATC_C
Cuts at: 0 113 2472
Size: 113 2359
BanI G'GyrC_C
Cuts at: 0 325 2472
Size: 325 2147
BanII G_rGCy'C
Cuts at: 0 299 2250 2472
Size: 299 1951 222
Fragments arranged by size:
1951 299 222
BbsI GAAGACnn'nnnn_
Cuts at: 0 1727 2164 2216 2472
Size: 1727 437 52 256
Fragments arranged by size:
1727 437 256 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 33 67 2472
Size: 33 34 2405
Fragments arranged by size:
2405 34 33
BfrBI ATG'CAT
Cuts at: 0 1182 2460 2472
Size: 1182 1278 12
Fragments arranged by size:
1278 1182 12
BlpI GC'TnA_GC
Cuts at: 0 59 2192 2472
Size: 59 2133 280
Fragments arranged by size:
2133 280 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 1615 2472
Size: 1615 857
BmtI G_CTAG'C
Cuts at: 0 2183 2472
Size: 2183 289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 270 2472
Size: 270 2202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1967 2264 2349 2472
Size: 241 1726 297 85 123
Fragments arranged by size:
1726 297 241 123 85
Bpu10I CC'TnA_GC
Cuts at: 0 30 128 2472
Size: 30 98 2344
Fragments arranged by size:
2344 98 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1965 2472
Size: 253 1712 507
Fragments arranged by size:
1712 507 253
BsaBI GATnn'nnATC
Cuts at: 0 400 2306 2472
Size: 400 1906 166
Fragments arranged by size:
1906 400 166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2335 2472
Size: 2335 137
BseYI C'CCAG_C
Cuts at: 0 282 2472
Size: 282 2190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 283 2472
Size: 94 189 2189
Fragments arranged by size:
2189 189 94
BsiEI CG_ry'CG
Cuts at: 0 185 214 239 2472
Size: 185 29 25 2233
Fragments arranged by size:
2233 185 29 25
BsiHKAI G_wGCw'C
Cuts at: 0 966 2472
Size: 966 1506
BsmI GAATG_Cn'
Cuts at: 0 2397 2472
Size: 2397 75
Bsp1286I G_dGCh'C
Cuts at: 0 299 966 2250 2472
Size: 299 667 1284 222
Fragments arranged by size:
1284 667 299 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 2472
Size: 66 2406
BsrBI CCG'CTC
Cuts at: 0 127 2472
Size: 127 2345
BsrDI GCAATG_nn'
Cuts at: 0 1074 2472
Size: 1074 1398
BsrFI r'CCGG_y
Cuts at: 0 54 180 213 238 258 2472
Size: 54 126 33 25 20 2214
Fragments arranged by size:
2214 126 54 33 25 20
BsrGI T'GTAC_A
Cuts at: 0 429 2472
Size: 429 2043
BssSI C'ACGA_G
Cuts at: 0 1109 2472
Size: 1109 1363
BstEII G'GTnAC_C
Cuts at: 0 1468 2472
Size: 1468 1004
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 360 1166 2472
Size: 282 78 806 1306
Fragments arranged by size:
1306 806 282 78
BstYI r'GATC_y
Cuts at: 0 113 132 556 777 1311 2472
Size: 113 19 424 221 534 1161
Fragments arranged by size:
1161 534 424 221 113 19
BstZ17I GTA'TAC
Cuts at: 0 613 2472
Size: 613 1859
BtgI C'CryG_G
Cuts at: 0 145 2472
Size: 145 2327
BtsI GCAGTG_nn'
Cuts at: 0 44 196 2472
Size: 44 152 2276
Fragments arranged by size:
2276 152 44
DraI TTT'AAA
Cuts at: 0 1743 2472
Size: 1743 729
EaeI y'GGCC_r
Cuts at: 0 68 182 211 236 1200 2472
Size: 68 114 29 25 964 1272
Fragments arranged by size:
1272 964 114 68 29 25
EagI C'GGCC_G
Cuts at: 0 182 211 236 2472
Size: 182 29 25 2236
Fragments arranged by size:
2236 182 29 25
EarI CTCTTCn'nnn_
Cuts at: 0 1622 1995 2034 2472
Size: 1622 373 39 438
Fragments arranged by size:
1622 438 373 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 190 2472
Size: 190 2282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1084 1967 2214 2264 2349 2472
Size: 241 843 883 247 50 85 123
Fragments arranged by size:
883 843 247 241 123 85 50
EcoO109I rG'GnC_Cy
Cuts at: 0 78 251 503 1852 2472
Size: 78 173 252 1349 620
Fragments arranged by size:
1349 620 252 173 78
EcoRI G'AATT_C
Cuts at: 0 798 917 1641 2472
Size: 798 119 724 831
Fragments arranged by size:
831 798 724 119
EcoRV GAT'ATC
Cuts at: 0 981 2472
Size: 981 1491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2006 2034 2472
Size: 2006 28 438
Fragments arranged by size:
2006 438 28
HaeII r_GCGC'y
Cuts at: 0 291 2472
Size: 291 2181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 393 425 1499 1531 1606 1638
Size: 178 32 183 32 1074 32 75 32
Cuts at: 1638 1713 1745 1857 1889 2041 2073 2472
Size: 75 32 112 32 152 32 399
Fragments arranged by size:
1074 399 183 178 152 112 75 75
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1766 2472
Size: 1766 706
HindIII A'AGCT_T
Cuts at: 0 643 932 1543 1697 2472
Size: 643 289 611 154 775
Fragments arranged by size:
775 643 611 289 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 2392 2472
Size: 534 1858 80
Fragments arranged by size:
1858 534 80
MslI CAynn'nnrTG
Cuts at: 0 1382 2472
Size: 1382 1090
MspA1I CmG'CkG
Cuts at: 0 147 282 2043 2472
Size: 147 135 1761 429
Fragments arranged by size:
1761 429 147 135
NaeI GCC'GGC
Cuts at: 0 56 182 240 2472
Size: 56 126 58 2232
Fragments arranged by size:
2232 126 58 56
NdeI CA'TA_TG
Cuts at: 0 1383 2472
Size: 1383 1089
NgoMIV G'CCGG_C
Cuts at: 0 54 180 238 2472
Size: 54 126 58 2234
Fragments arranged by size:
2234 126 58 54
NheI G'CTAG_C
Cuts at: 0 2179 2472
Size: 2179 293
NsiI A_TGCA'T
Cuts at: 0 1184 2462 2472
Size: 1184 1278 10
Fragments arranged by size:
1278 1184 10
NspI r_CATG'y
Cuts at: 0 67 1182 2472
Size: 67 1115 1290
Fragments arranged by size:
1290 1115 67
PasI CC'CwG_GG
Cuts at: 0 1855 2472
Size: 1855 617
PflMI CCAn_nnn'nTGG
Cuts at: 0 2101 2472
Size: 2101 371
PfoI T'CCnGG_A
Cuts at: 0 665 2472
Size: 665 1807
PpuMI rG'GwC_Cy
Cuts at: 0 78 503 2472
Size: 78 425 1969
Fragments arranged by size:
1969 425 78
PvuII CAG'CTG
Cuts at: 0 2043 2472
Size: 2043 429
RsrII CG'GwC_CG
Cuts at: 0 122 2472
Size: 122 2350
SacII CC_GC'GG
Cuts at: 0 148 2472
Size: 148 2324
SanDI GG'GwC_CC
Cuts at: 0 503 2472
Size: 503 1969
SapI GCTCTTCn'nnn_
Cuts at: 0 1622 2034 2472
Size: 1622 412 438
Fragments arranged by size:
1622 438 412
ScaI AGT'ACT
Cuts at: 0 2326 2472
Size: 2326 146
SexAI A'CCwGG_T
Cuts at: 0 1685 2472
Size: 1685 787
SfcI C'TryA_G
Cuts at: 0 2340 2472
Size: 2340 132
SgrAI Cr'CCGG_yG
Cuts at: 0 258 2472
Size: 258 2214
SmaI CCC'GGG
Cuts at: 0 102 2472
Size: 102 2370
SmlI C'TyrA_G
Cuts at: 0 268 384 1980 2472
Size: 268 116 1596 492
Fragments arranged by size:
1596 492 268 116
SspI AAT'ATT
Cuts at: 0 2359 2472
Size: 2359 113
TatI w'GTAC_w
Cuts at: 0 429 767 2324 2472
Size: 429 338 1557 148
Fragments arranged by size:
1557 429 338 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 324 356 1465 1497 2103 2132 2135 2164
Size: 324 32 1109 32 606 29 3 29
Cuts at: 2164 2472
Size: 308
Fragments arranged by size:
1109 606 324 308 32 32 29 29
3
XbaI T'CTAG_A
Cuts at: 0 1486 2472
Size: 1486 986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 1567 2472
Size: 357 1210 905
Fragments arranged by size:
1210 905 357
XmaI C'CCGG_G
Cuts at: 0 100 2472
Size: 100 2372
XmnI GAAnn'nnTTC
Cuts at: 0 1494 1989 2472
Size: 1494 495 483
Fragments arranged by size:
1494 495 483
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AloI AlwNI ApoI
AvaI BaeI BamHI BanI BanII BbsI BcgI BfrBI
BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI
BstZ17I BtgI BtsI DraI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII MmeI MslI MspA1I NaeI NdeI NgoMIV NheI
NsiI NspI PasI PflMI PfoI PpuMI PvuII RsrII
SacII SanDI SapI ScaI SexAI SfcI SgrAI SmaI
SmlI SspI TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BbeI BbvCI
BciVI BclI BglI BglII Bme1580I BmgBI BsaI BsaAI
BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BssHII
BstAPI BstBI Bsu36I BtgZI ClaI CspCI DraIII DrdI
EcoICRI EcoNI FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NotI NruI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI SacI SalI SbfI SfiI SfoI
SgfI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII Tth111I XhoI ZraI