Restriction map for
pFN21AB0406
(Linear) (Six-base) MAPSORT of: KIBB0406.seq Check: 786 from: 1 to: 2427
KIBB0406.orf
With 182 enzymes: SgfI *
July 3, 2012 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 72 2427
Size: 72 2355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 794 845 961 2427
Size: 523 271 51 116 1466
Fragments arranged by size:
1466 523 271 116 51
AleI CACnn'nnGTG
Cuts at: 0 956 2427
Size: 956 1471
AlwNI CAG_nnn'CTG
Cuts at: 0 22 276 483 1461 2339 2427
Size: 22 254 207 978 878 88
Fragments arranged by size:
978 878 254 207 88 22
ApaI G_GGCC'C
Cuts at: 0 972 2427
Size: 972 1455
ApaLI G'TGCA_C
Cuts at: 0 875 2427
Size: 875 1552
ApoI r'AATT_y
Cuts at: 0 1593 1706 2427
Size: 1593 113 721
Fragments arranged by size:
1593 721 113
AvaI C'yCGr_G
Cuts at: 0 1043 2427
Size: 1043 1384
BamHI G'GATC_C
Cuts at: 0 1788 2427
Size: 1788 639
BanII G_rGCy'C
Cuts at: 0 299 972 2427
Size: 299 673 1455
Fragments arranged by size:
1455 673 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 658 2427
Size: 319 339 1769
Fragments arranged by size:
1769 339 319
BciVI GTATCCnnnnn_n'
Cuts at: 0 1868 2427
Size: 1868 559
BfrBI ATG'CAT
Cuts at: 0 2320 2427
Size: 2320 107
Bme1580I G_kGCm'C
Cuts at: 0 879 972 2427
Size: 879 93 1455
Fragments arranged by size:
1455 879 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 622 1249 2427
Size: 622 627 1178
Fragments arranged by size:
1178 627 622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 728 2427
Size: 123 605 1699
Fragments arranged by size:
1699 605 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 286 346 376 460 565 2427
Size: 283 3 60 30 84 105 1862
Fragments arranged by size:
1862 283 105 84 60 30 3
Bpu10I CC'TnA_GC
Cuts at: 0 740 2427
Size: 740 1687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1219 2427
Size: 512 707 1208
Fragments arranged by size:
1208 707 512
BsaI GGTCTCn'nnnn_
Cuts at: 0 213 1560 2427
Size: 213 1347 867
Fragments arranged by size:
1347 867 213
BsaBI GATnn'nnATC
Cuts at: 0 1787 1793 2427
Size: 1787 6 634
Fragments arranged by size:
1787 634 6
BsaWI w'CCGG_w
Cuts at: 0 222 2427
Size: 222 2205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 2427
Size: 70 30 2327
Fragments arranged by size:
2327 70 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 2427
Size: 352 2075
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 2427
Size: 1498 929
BsiHKAI G_wGCw'C
Cuts at: 0 299 879 2095 2427
Size: 299 580 1216 332
Fragments arranged by size:
1216 580 332 299
BsmI GAATG_Cn'
Cuts at: 0 1657 2206 2427
Size: 1657 549 221
Fragments arranged by size:
1657 549 221
Bsp1286I G_dGCh'C
Cuts at: 0 299 879 972 2095 2427
Size: 299 580 93 1123 332
Fragments arranged by size:
1123 580 332 299 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 72 1520 2427
Size: 72 1448 907
Fragments arranged by size:
1448 907 72
BstBI TT'CG_AA
Cuts at: 0 2239 2427
Size: 2239 188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2103 2427
Size: 2103 324
BstYI r'GATC_y
Cuts at: 0 66 1115 1788 2427
Size: 66 1049 673 639
Fragments arranged by size:
1049 673 639 66
Bsu36I CC'TnA_GG
Cuts at: 0 852 2427
Size: 852 1575
BtgI C'CryG_G
Cuts at: 0 564 2427
Size: 564 1863
BtsI GCAGTG_nn'
Cuts at: 0 350 2427
Size: 350 2077
DraI TTT'AAA
Cuts at: 0 804 2427
Size: 804 1623
EaeI y'GGCC_r
Cuts at: 0 1530 2427
Size: 1530 897
EarI CTCTTCn'nnn_
Cuts at: 0 2002 2427
Size: 2002 425
EcoICRI GAG'CTC
Cuts at: 0 297 2427
Size: 297 2130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 286 346 376 460 523 565 794
Size: 283 3 60 30 84 63 42 229
Cuts at: 794 845 961 2427
Size: 51 116 1466
Fragments arranged by size:
1466 283 229 116 84 63 60 51
42 30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 2427
Size: 289 2138
EcoO109I rG'GnC_Cy
Cuts at: 0 19 635 2427
Size: 19 616 1792
Fragments arranged by size:
1792 616 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 2427
Size: 61 2366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 306 338 679 711 747 779 1460 1492
Size: 306 32 341 32 36 32 681 32
Cuts at: 1492 2427
Size: 935
Fragments arranged by size:
935 681 341 306 36 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2173 2427
Size: 2173 254
HindIII A'AGCT_T
Cuts at: 0 8 2427
Size: 8 2419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 2427
Size: 1147 1280
MscI TGG'CCA
Cuts at: 0 1532 2427
Size: 1532 895
MslI CAynn'nnrTG
Cuts at: 0 956 2427
Size: 956 1471
NdeI CA'TA_TG
Cuts at: 0 1994 2427
Size: 1994 433
NsiI A_TGCA'T
Cuts at: 0 2322 2427
Size: 2322 105
NspI r_CATG'y
Cuts at: 0 426 978 2427
Size: 426 552 1449
Fragments arranged by size:
1449 552 426
PflMI CCAn_nnn'nTGG
Cuts at: 0 645 2427
Size: 645 1782
PfoI T'CCnGG_A
Cuts at: 0 581 2427
Size: 581 1846
PpuMI rG'GwC_Cy
Cuts at: 0 635 2427
Size: 635 1792
PspOMI G'GGCC_C
Cuts at: 0 968 2427
Size: 968 1459
PspXI vC'TCGA_Gb
Cuts at: 0 1043 2427
Size: 1043 1384
PstI C_TGCA'G
Cuts at: 0 687 1005 2427
Size: 687 318 1422
Fragments arranged by size:
1422 687 318
SacI G_AGCT'C
Cuts at: 0 299 2427
Size: 299 2128
ScaI AGT'ACT
Cuts at: 0 897 2427
Size: 897 1530
SexAI A'CCwGG_T
Cuts at: 0 1460 2427
Size: 1460 967
SfcI C'TryA_G
Cuts at: 0 608 683 1001 2427
Size: 608 75 318 1426
Fragments arranged by size:
1426 608 318 75
SmlI C'TyrA_G
Cuts at: 0 527 1043 1234 2427
Size: 527 516 191 1193
Fragments arranged by size:
1193 527 516 191
SphI G_CATG'C
Cuts at: 0 978 2427
Size: 978 1449
StuI AGG'CCT
Cuts at: 0 397 1067 2427
Size: 397 670 1360
Fragments arranged by size:
1360 670 397
StyI C'CwwG_G
Cuts at: 0 236 290 1062 2366 2427
Size: 236 54 772 1304 61
Fragments arranged by size:
1304 772 236 61 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 443 968 1978 2427
Size: 443 525 1010 449
Fragments arranged by size:
1010 525 449 443
TatI w'GTAC_w
Cuts at: 0 895 2427
Size: 895 1532
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 711 743 970 1002 1357 1389
Size: 70 32 609 32 227 32 355 32
Cuts at: 1389 1802 1834 2427
Size: 413 32 593
Fragments arranged by size:
609 593 413 355 227 70 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 363 2358 2427
Size: 363 1995 69
Fragments arranged by size:
1995 363 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 484 547 962 1163 1675 2427
Size: 484 63 415 201 512 752
Fragments arranged by size:
752 512 484 415 201 63
XhoI C'TCGA_G
Cuts at: 0 1043 2427
Size: 1043 1384
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanII BbsI BciVI BfrBI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspMI BstBI BstXI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI
MslI NdeI NsiI NspI PflMI PfoI PpuMI PspOMI
PspXI PstI SacI ScaI SexAI SfcI SmlI SphI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BclI BglI BglII BlpI
BmgBI BmtI BsaAI BsaHI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XmaI XmnI ZraI