Restriction map for
pFN21AB0402
(Linear) (Six-base) MAPSORT of: KIBB0402.seq Check: 8539 from: 1 to: 1932
KIBB0402.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1239 1932
Size: 1239 693
AccI GT'mk_AC
Cuts at: 0 1281 1932
Size: 1281 651
Acc65I G'GTAC_C
Cuts at: 0 1071 1932
Size: 1071 861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 200 1079 1604 1932
Size: 142 58 879 525 328
Fragments arranged by size:
879 525 328 142 58
AflIII A'CryG_T
Cuts at: 0 206 979 1932
Size: 206 773 953
Fragments arranged by size:
953 773 206
AgeI A'CCGG_T
Cuts at: 0 676 1932
Size: 676 1256
AhdI GACnn_n'nnGTC
Cuts at: 0 654 1932
Size: 654 1278
AlwNI CAG_nnn'CTG
Cuts at: 0 310 1932
Size: 310 1622
ApaI G_GGCC'C
Cuts at: 0 400 555 1932
Size: 400 155 1377
Fragments arranged by size:
1377 400 155
ApoI r'AATT_y
Cuts at: 0 352 627 1088 1932
Size: 352 275 461 844
Fragments arranged by size:
844 461 352 275
AseI AT'TA_AT
Cuts at: 0 1874 1932
Size: 1874 58
BamHI G'GATC_C
Cuts at: 0 1760 1932
Size: 1760 172
BanI G'GyrC_C
Cuts at: 0 1071 1227 1361 1932
Size: 1071 156 134 571
Fragments arranged by size:
1071 571 156 134
BanII G_rGCy'C
Cuts at: 0 400 460 473 555 1932
Size: 400 60 13 82 1377
Fragments arranged by size:
1377 400 82 60 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1592 1627 1932
Size: 1592 35 305
Fragments arranged by size:
1592 305 35
BfrBI ATG'CAT
Cuts at: 0 697 1932
Size: 697 1235
Bme1580I G_kGCm'C
Cuts at: 0 400 555 1364 1932
Size: 400 155 809 568
Fragments arranged by size:
809 568 400 155
BmrI ACTGGGnnnn_n'
Cuts at: 0 408 1126 1299 1932
Size: 408 718 173 633
Fragments arranged by size:
718 633 408 173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 332 371 487 629 853 1130 1932
Size: 15 317 39 116 142 224 277 802
Fragments arranged by size:
802 317 277 224 142 116 39 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 1015 1932
Size: 1015 917
BsaBI GATnn'nnATC
Cuts at: 0 1442 1932
Size: 1442 490
BsaHI Gr'CG_yC
Cuts at: 0 1848 1932
Size: 1848 84
BsaWI w'CCGG_w
Cuts at: 0 676 1932
Size: 676 1256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1813 1843 1932
Size: 1813 30 89
Fragments arranged by size:
1813 89 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 354 850 1932
Size: 354 496 1082
Fragments arranged by size:
1082 496 354
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1589 1932
Size: 1217 372 343
Fragments arranged by size:
1217 372 343
BsmI GAATG_Cn'
Cuts at: 0 322 488 1932
Size: 322 166 1444
Fragments arranged by size:
1444 322 166
Bsp1286I G_dGCh'C
Cuts at: 0 400 460 473 555 1364 1932
Size: 400 60 13 82 809 568
Fragments arranged by size:
809 568 400 82 60 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 382 1239 1932
Size: 382 857 693
Fragments arranged by size:
857 693 382
BsrBI CCG'CTC
Cuts at: 0 1248 1932
Size: 1248 684
BsrDI GCAATG_nn'
Cuts at: 0 90 1703 1932
Size: 90 1613 229
Fragments arranged by size:
1613 229 90
BsrFI r'CCGG_y
Cuts at: 0 676 1932
Size: 676 1256
BsrGI T'GTAC_A
Cuts at: 0 685 768 1932
Size: 685 83 1164
Fragments arranged by size:
1164 685 83
BstBI TT'CG_AA
Cuts at: 0 585 1932
Size: 585 1347
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1357 1932
Size: 1357 575
BstYI r'GATC_y
Cuts at: 0 1760 1932
Size: 1760 172
BtgI C'CryG_G
Cuts at: 0 329 359 1223 1326 1386 1476 1506 1932
Size: 329 30 864 103 60 90 30 426
Fragments arranged by size:
864 426 329 103 90 60 30 30
ClaI AT'CG_AT
Cuts at: 0 704 1932
Size: 704 1228
DraI TTT'AAA
Cuts at: 0 102 1932
Size: 102 1830
EaeI y'GGCC_r
Cuts at: 0 965 1035 1323 1329 1383 1503 1932
Size: 965 70 288 6 54 120 429
Fragments arranged by size:
965 429 288 120 70 54 6
EarI CTCTTCn'nnn_
Cuts at: 0 331 532 995 1238 1584 1932
Size: 331 201 463 243 346 348
Fragments arranged by size:
463 348 346 331 243 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 142 200 332 371 487 629 853
Size: 15 127 58 132 39 116 142 224
Cuts at: 853 1079 1130 1604 1932
Size: 226 51 474 328
Fragments arranged by size:
474 328 226 224 142 132 127 116
58 51 39 15
EcoO109I rG'GnC_Cy
Cuts at: 0 396 397 1932
Size: 396 1 1535
Fragments arranged by size:
1535 396 1
EcoRV GAT'ATC
Cuts at: 0 1770 1932
Size: 1770 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 831 863 1813 1845 1932
Size: 640 32 159 32 950 32 87
Fragments arranged by size:
950 640 159 87 32 32 32
HincII GTy'rAC
Cuts at: 0 1007 1104 1111 1932
Size: 1007 97 7 821
Fragments arranged by size:
1007 821 97 7
HindIII A'AGCT_T
Cuts at: 0 862 1932
Size: 862 1070
KpnI G_GTAC'C
Cuts at: 0 1075 1932
Size: 1075 857
MfeI C'AATT_G
Cuts at: 0 599 1932
Size: 599 1333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 1903 1932
Size: 1534 369 29
Fragments arranged by size:
1534 369 29
MscI TGG'CCA
Cuts at: 0 967 1037 1325 1331 1385 1505 1932
Size: 967 70 288 6 54 120 427
Fragments arranged by size:
967 427 288 120 70 54 6
MslI CAynn'nnrTG
Cuts at: 0 1222 1337 1343 1493 1499 1888 1932
Size: 1222 115 6 150 6 389 44
Fragments arranged by size:
1222 389 150 115 44 6 6
NcoI C'CATG_G
Cuts at: 0 1223 1326 1386 1476 1932
Size: 1223 103 60 90 456
Fragments arranged by size:
1223 456 103 90 60
NsiI A_TGCA'T
Cuts at: 0 699 1932
Size: 699 1233
NspI r_CATG'y
Cuts at: 0 983 1932
Size: 983 949
PasI CC'CwG_GG
Cuts at: 0 1076 1932
Size: 1076 856
PciI A'CATG_T
Cuts at: 0 979 1932
Size: 979 953
PfoI T'CCnGG_A
Cuts at: 0 1842 1932
Size: 1842 90
PspOMI G'GGCC_C
Cuts at: 0 396 551 1932
Size: 396 155 1381
Fragments arranged by size:
1381 396 155
SapI GCTCTTCn'nnn_
Cuts at: 0 1238 1584 1932
Size: 1238 346 348
Fragments arranged by size:
1238 348 346
ScaI AGT'ACT
Cuts at: 0 496 1593 1932
Size: 496 1097 339
Fragments arranged by size:
1097 496 339
SspI AAT'ATT
Cuts at: 0 949 1932
Size: 949 983
StuI AGG'CCT
Cuts at: 0 1143 1932
Size: 1143 789
StyI C'CwwG_G
Cuts at: 0 986 1223 1326 1350 1380 1386 1455 1476
Size: 986 237 103 24 30 6 69 21
Cuts at: 1476 1932
Size: 456
Fragments arranged by size:
986 456 237 103 69 30 24 21
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1024 1653 1932
Size: 1024 629 279
Fragments arranged by size:
1024 629 279
TatI w'GTAC_w
Cuts at: 0 284 494 685 768 924 1591 1932
Size: 284 210 191 83 156 667 341
Fragments arranged by size:
667 341 284 210 191 156 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 789 1372 1932
Size: 789 583 560
Fragments arranged by size:
789 583 560
XmnI GAAnn'nnTTC
Cuts at: 0 723 1932
Size: 723 1209
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AlwNI
ApaI ApoI AseI BamHI BanI BanII BbsI BfrBI
Bme1580I BmrI BpmI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BsgI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BstBI BstXI BstYI BtgI ClaI DraI EaeI
EarI Eco57MI EcoO109I EcoRV Hin4I HincII HindIII KpnI
MfeI MmeI MscI MslI NcoI NsiI NspI PasI
PciI PfoI PspOMI SapI ScaI SspI StuI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AleI AlfI AloI ApaLI
AscI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BseYI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I
BtgZI BtsI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HaeII HpaI
KasI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XbaI XhoI XmaI ZraI