Restriction map for
pFN21AA1354
(Linear) (Six-base) MAPSORT of: KIAA1354.seq Check: 5632 from: 1 to: 1851
KIAA1354.orf
With 182 enzymes: SgfI *
March 22, 2012 15:29 ..
Acc65I G'GTAC_C
Cuts at: 0 162 1851
Size: 162 1689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 349 553 1491 1811 1823 1851
Size: 328 21 204 938 320 12 28
Fragments arranged by size:
938 328 320 204 28 21 12
AflII C'TTAA_G
Cuts at: 0 619 1851
Size: 619 1232
AleI CACnn'nnGTG
Cuts at: 0 1181 1851
Size: 1181 670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1040 1851
Size: 1040 811
AlwNI CAG_nnn'CTG
Cuts at: 0 930 1851
Size: 930 921
ApoI r'AATT_y
Cuts at: 0 801 1851
Size: 801 1050
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 146 179 1851
Size: 146 33 1672
Fragments arranged by size:
1672 146 33
BanI G'GyrC_C
Cuts at: 0 162 1851
Size: 162 1689
BanII G_rGCy'C
Cuts at: 0 1388 1851
Size: 1388 463
BbsI GAAGACnn'nnnn_
Cuts at: 0 683 1851
Size: 683 1168
BclI T'GATC_A
Cuts at: 0 120 1851
Size: 120 1731
BglII A'GATC_T
Cuts at: 0 982 1851
Size: 982 869
Bme1580I G_kGCm'C
Cuts at: 0 972 1188 1851
Size: 972 216 663
Fragments arranged by size:
972 663 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 569 1851
Size: 569 1282
BmtI G_CTAG'C
Cuts at: 0 389 821 1851
Size: 389 432 1030
Fragments arranged by size:
1030 432 389
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1498 1851
Size: 1498 353
Bpu10I CC'TnA_GC
Cuts at: 0 667 1851
Size: 667 1184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1200 1851
Size: 1200 651
BsaAI yAC'GTr
Cuts at: 0 759 1851
Size: 759 1092
BsaHI Gr'CG_yC
Cuts at: 0 32 211 1851
Size: 32 179 1640
Fragments arranged by size:
1640 179 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1607 1637 1851
Size: 1607 30 214
Fragments arranged by size:
1607 214 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 926 1851
Size: 926 925
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1244 1851
Size: 781 463 607
Fragments arranged by size:
781 607 463
BsiHKAI G_wGCw'C
Cuts at: 0 1388 1851
Size: 1388 463
BsmI GAATG_Cn'
Cuts at: 0 1757 1851
Size: 1757 94
BsmBI CGTCTCn'nnnn_
Cuts at: 0 39 1851
Size: 39 1812
Bsp1286I G_dGCh'C
Cuts at: 0 972 1188 1388 1851
Size: 972 216 200 463
Fragments arranged by size:
972 463 216 200
BspHI T'CATG_A
Cuts at: 0 776 1851
Size: 776 1075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 802 1131 1851
Size: 802 329 720
Fragments arranged by size:
802 720 329
BsrDI GCAATG_nn'
Cuts at: 0 1455 1851
Size: 1455 396
BsrGI T'GTAC_A
Cuts at: 0 1465 1768 1851
Size: 1465 303 83
Fragments arranged by size:
1465 303 83
BstEII G'GTnAC_C
Cuts at: 0 1805 1851
Size: 1805 46
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 1851
Size: 897 954
BstYI r'GATC_y
Cuts at: 0 744 982 1851
Size: 744 238 869
Fragments arranged by size:
869 744 238
BtsI GCAGTG_nn'
Cuts at: 0 1227 1851
Size: 1227 624
DraIII CAC_nnn'GTG
Cuts at: 0 1753 1851
Size: 1753 98
EaeI y'GGCC_r
Cuts at: 0 1238 1851
Size: 1238 613
EcoICRI GAG'CTC
Cuts at: 0 1386 1851
Size: 1386 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 349 553 1491 1811 1823 1851
Size: 328 21 204 938 320 12 28
Fragments arranged by size:
938 328 320 204 28 21 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 28 160 448 1851
Size: 28 132 288 1403
Fragments arranged by size:
1403 288 132 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 167 199 1607 1639 1851
Size: 167 32 1408 32 212
Fragments arranged by size:
1408 212 167 32 32
HindIII A'AGCT_T
Cuts at: 0 280 1851
Size: 280 1571
KpnI G_GTAC'C
Cuts at: 0 166 1851
Size: 166 1685
MfeI C'AATT_G
Cuts at: 0 1580 1851
Size: 1580 271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 653 1452 1593 1851
Size: 450 203 799 141 258
Fragments arranged by size:
799 450 258 203 141
MscI TGG'CCA
Cuts at: 0 1240 1851
Size: 1240 611
MslI CAynn'nnrTG
Cuts at: 0 975 1181 1202 1851
Size: 975 206 21 649
Fragments arranged by size:
975 649 206 21
MspA1I CmG'CkG
Cuts at: 0 930 1228 1851
Size: 930 298 623
Fragments arranged by size:
930 623 298
NheI G'CTAG_C
Cuts at: 0 385 817 1851
Size: 385 432 1034
Fragments arranged by size:
1034 432 385
NspI r_CATG'y
Cuts at: 0 1464 1851
Size: 1464 387
PasI CC'CwG_GG
Cuts at: 0 1801 1851
Size: 1801 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 172 1851
Size: 172 1679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 626 658 1851
Size: 626 32 1193
Fragments arranged by size:
1193 626 32
PshAI GACnn'nnGTC
Cuts at: 0 1441 1851
Size: 1441 410
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 774 806 1851
Size: 774 32 1045
Fragments arranged by size:
1045 774 32
PvuII CAG'CTG
Cuts at: 0 930 1228 1851
Size: 930 298 623
Fragments arranged by size:
930 623 298
SacI G_AGCT'C
Cuts at: 0 1388 1851
Size: 1388 463
ScaI AGT'ACT
Cuts at: 0 558 1851
Size: 558 1293
SexAI A'CCwGG_T
Cuts at: 0 165 1851
Size: 165 1686
SfcI C'TryA_G
Cuts at: 0 1439 1851
Size: 1439 412
SmlI C'TyrA_G
Cuts at: 0 619 1179 1851
Size: 619 560 672
Fragments arranged by size:
672 619 560
SspI AAT'ATT
Cuts at: 0 401 717 1851
Size: 401 316 1134
Fragments arranged by size:
1134 401 316
StyI C'CwwG_G
Cuts at: 0 12 1851
Size: 12 1839
TatI w'GTAC_w
Cuts at: 0 556 1465 1768 1851
Size: 556 909 303 83
Fragments arranged by size:
909 556 303 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1767 1799 1851
Size: 1767 32 52
Fragments arranged by size:
1767 52 32
XbaI T'CTAG_A
Cuts at: 0 1813 1851
Size: 1813 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1609 1851
Size: 1609 242
Enzymes that do cut:
Acc65I AcuI AflII AleI AlfI AlwNI ApoI BaeI
BanI BanII BbsI BclI BglII Bme1580I BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI
BstEII BstXI BstYI BtsI DraIII EaeI EcoICRI Eco57MI
FalI Hin4I HindIII KpnI MfeI MmeI MscI MslI
MspA1I NheI NspI PasI PflMI PpiI PshAI PsrI
PvuII SacI ScaI SexAI SfcI SmlI SspI StyI
TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
BmgBI BpmI BsaI BsaBI BsaWI BseYI BsiEI BsiWI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI
EarI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI MluI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII Tth111I XhoI XmaI XmnI ZraI