Restriction map for
pFN21AA0928
(Linear) (Six-base) MAPSORT of: KIAA0928.seq Check: 6412 from: 1 to: 5766
KIAA0928.orf
With 182 enzymes: SgfI *
July 15, 2010 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1101 5484 5766
Size: 1101 4383 282
Fragments arranged by size:
4383 1101 282
AccI GT'mk_AC
Cuts at: 0 736 2740 5766
Size: 736 2004 3026
Fragments arranged by size:
3026 2004 736
AclI AA'CG_TT
Cuts at: 0 3463 5766
Size: 3463 2303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 2405 2729 3326 3877 4783 5766
Size: 249 2156 324 597 551 906 983
Fragments arranged by size:
2156 983 906 597 551 324 249
AfeI AGC'GCT
Cuts at: 0 3032 5107 5766
Size: 3032 2075 659
Fragments arranged by size:
3032 2075 659
AflII C'TTAA_G
Cuts at: 0 688 5766
Size: 688 5078
AhdI GACnn_n'nnGTC
Cuts at: 0 3444 5205 5766
Size: 3444 1761 561
Fragments arranged by size:
3444 1761 561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3481 4676 5766
Size: 3481 1195 1090
Fragments arranged by size:
3481 1195 1090
AlwNI CAG_nnn'CTG
Cuts at: 0 2614 2958 3096 4058 5590 5766
Size: 2614 344 138 962 1532 176
Fragments arranged by size:
2614 1532 962 344 176 138
ApoI r'AATT_y
Cuts at: 0 683 1027 1111 1296 1489 1519 2596 2683
Size: 683 344 84 185 193 30 1077 87
Cuts at: 2683 2875 3660 4824 5001 5113 5596 5665 5766
Size: 192 785 1164 177 112 483 69 101
Fragments arranged by size:
1164 1077 785 683 483 344 193 192
185 177 112 101 87 84 69 30
AseI AT'TA_AT
Cuts at: 0 3524 5766
Size: 3524 2242
AvaI C'yCGr_G
Cuts at: 0 1964 4817 5184 5766
Size: 1964 2853 367 582
Fragments arranged by size:
2853 1964 582 367
BamHI G'GATC_C
Cuts at: 0 4536 5766
Size: 4536 1230
BanI G'GyrC_C
Cuts at: 0 1662 5449 5766
Size: 1662 3787 317
Fragments arranged by size:
3787 1662 317
BanII G_rGCy'C
Cuts at: 0 3216 3841 4293 5294 5724 5766
Size: 3216 625 452 1001 430 42
Fragments arranged by size:
3216 1001 625 452 430 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 214 1727 2220 5171 5766
Size: 214 1513 493 2951 595
Fragments arranged by size:
2951 1513 595 493 214
BbvCI CC'TCA_GC
Cuts at: 0 25 5766
Size: 25 5741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2222 2256 5766
Size: 2222 34 3510
Fragments arranged by size:
3510 2222 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1686 5766
Size: 1686 4080
BclI T'GATC_A
Cuts at: 0 2292 2817 4335 4950 5766
Size: 2292 525 1518 615 816
Fragments arranged by size:
2292 1518 816 615 525
BfrBI ATG'CAT
Cuts at: 0 391 5766
Size: 391 5375
BglII A'GATC_T
Cuts at: 0 351 1751 3510 5371 5766
Size: 351 1400 1759 1861 395
Fragments arranged by size:
1861 1759 1400 395 351
BlpI GC'TnA_GC
Cuts at: 0 3469 4736 5766
Size: 3469 1267 1030
Fragments arranged by size:
3469 1267 1030
Bme1580I G_kGCm'C
Cuts at: 0 1665 4813 5766
Size: 1665 3148 953
Fragments arranged by size:
3148 1665 953
BmrI ACTGGGnnnn_n'
Cuts at: 0 2723 4765 5766
Size: 2723 2042 1001
Fragments arranged by size:
2723 2042 1001
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1104 4751 5766
Size: 1104 3647 1015
Fragments arranged by size:
3647 1104 1015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2486 2643 3955 4348 5035 5500 5766
Size: 2486 157 1312 393 687 465 266
Fragments arranged by size:
2486 1312 687 465 393 266 157
Bpu10I CC'TnA_GC
Cuts at: 0 25 201 5288 5365 5766
Size: 25 176 5087 77 401
Fragments arranged by size:
5087 401 176 77 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1776 2548 3796 3843 5350 5766
Size: 340 1436 772 1248 47 1507 416
Fragments arranged by size:
1507 1436 1248 772 416 340 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 1528 2191 5766
Size: 1528 663 3575
Fragments arranged by size:
3575 1528 663
BsaBI GATnn'nnATC
Cuts at: 0 2279 5766
Size: 2279 3487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4373 4403 5766
Size: 4373 30 1363
Fragments arranged by size:
4373 1363 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1027 2185 3217 3327 3411 3846 4267 4270
Size: 1027 1158 1032 110 84 435 421 3
Cuts at: 4270 4279 4282 4285 5392 5766
Size: 9 3 3 1107 374
Fragments arranged by size:
1158 1107 1032 1027 435 421 374 110
84 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 3163 3686 3691 4096 4698 5766
Size: 3163 523 5 405 602 1068
Fragments arranged by size:
3163 1068 602 523 405 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1003 2089 2573 3380 5338 5766
Size: 971 32 1086 484 807 1958 428
Fragments arranged by size:
1958 1086 971 807 484 428 32
BsiHKAI G_wGCw'C
Cuts at: 0 1085 1530 3812 4772 5294 5766
Size: 1085 445 2282 960 522 472
Fragments arranged by size:
2282 1085 960 522 472 445
BsmI GAATG_Cn'
Cuts at: 0 2723 5766
Size: 2723 3043
Bsp1286I G_dGCh'C
Cuts at: 0 1085 1530 1665 3216 3812 3841 4293 4772
Size: 1085 445 135 1551 596 29 452 479
Cuts at: 4772 4813 5294 5724 5766
Size: 41 481 430 42
Fragments arranged by size:
1551 1085 596 481 479 452 445 430
135 42 41 29
BspHI T'CATG_A
Cuts at: 0 47 111 3435 5766
Size: 47 64 3324 2331
Fragments arranged by size:
3324 2331 64 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1101 1550 2595 5210 5484 5766
Size: 1101 449 1045 2615 274 282
Fragments arranged by size:
2615 1101 1045 449 282 274
BsrBI CCG'CTC
Cuts at: 0 3939 5766
Size: 3939 1827
BsrDI GCAATG_nn'
Cuts at: 0 4529 5766
Size: 4529 1237
BsrGI T'GTAC_A
Cuts at: 0 861 2453 2849 5766
Size: 861 1592 396 2917
Fragments arranged by size:
2917 1592 861 396
BssSI C'ACGA_G
Cuts at: 0 1868 5766
Size: 1868 3898
BstBI TT'CG_AA
Cuts at: 0 895 5766
Size: 895 4871
BstEII G'GTnAC_C
Cuts at: 0 2472 5766
Size: 2472 3294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2667 4104 5460 5766
Size: 2667 1437 1356 306
Fragments arranged by size:
2667 1437 1356 306
BstYI r'GATC_y
Cuts at: 0 351 1401 1751 3510 4536 5371 5766
Size: 351 1050 350 1759 1026 835 395
Fragments arranged by size:
1759 1050 1026 835 395 351 350
BstZ17I GTA'TAC
Cuts at: 0 737 2741 5766
Size: 737 2004 3025
Fragments arranged by size:
3025 2004 737
Bsu36I CC'TnA_GG
Cuts at: 0 1054 4464 5750 5766
Size: 1054 3410 1286 16
Fragments arranged by size:
3410 1286 1054 16
BtgI C'CryG_G
Cuts at: 0 91 5170 5420 5766
Size: 91 5079 250 346
Fragments arranged by size:
5079 346 250 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3242 3710 5222 5766
Size: 3242 468 1512 544
Fragments arranged by size:
3242 1512 544 468
BtsI GCAGTG_nn'
Cuts at: 0 2245 2956 3051 3183 4660 5766
Size: 2245 711 95 132 1477 1106
Fragments arranged by size:
2245 1477 1106 711 132 95
ClaI AT'CG_AT
Cuts at: 0 1629 2829 5766
Size: 1629 1200 2937
Fragments arranged by size:
2937 1629 1200
DraI TTT'AAA
Cuts at: 0 629 2601 2682 2766 3968 5670 5766
Size: 629 1972 81 84 1202 1702 96
Fragments arranged by size:
1972 1702 1202 629 96 84 81
DraIII CAC_nnn'GTG
Cuts at: 0 1075 5766
Size: 1075 4691
DrdI GACnn_nn'nnGTC
Cuts at: 0 721 5629 5766
Size: 721 4908 137
Fragments arranged by size:
4908 721 137
EaeI y'GGCC_r
Cuts at: 0 1886 2417 3773 4877 5515 5766
Size: 1886 531 1356 1104 638 251
Fragments arranged by size:
1886 1356 1104 638 531 251
EarI CTCTTCn'nnn_
Cuts at: 0 1073 1499 1562 1647 2027 2162 2189 3049
Size: 1073 426 63 85 380 135 27 860
Cuts at: 3049 3345 4295 4896 5299 5381 5390 5712 5766
Size: 296 950 601 403 82 9 322 54
Fragments arranged by size:
1073 950 860 601 426 403 380 322
296 135 85 82 63 54 27 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2382 5766
Size: 2382 3384
EcoICRI GAG'CTC
Cuts at: 0 5292 5766
Size: 5292 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 2405 2486 2643 2729 3326 3877 3955
Size: 249 2156 81 157 86 597 551 78
Cuts at: 3955 4348 4783 5035 5500 5766
Size: 393 435 252 465 266
Fragments arranged by size:
2156 597 551 465 435 393 266 252
249 157 86 81 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5050 5766
Size: 5050 716
EcoO109I rG'GnC_Cy
Cuts at: 0 73 940 4803 5190 5766
Size: 73 867 3863 387 576
Fragments arranged by size:
3863 867 576 387 73
EcoRI G'AATT_C
Cuts at: 0 1489 5113 5766
Size: 1489 3624 653
Fragments arranged by size:
3624 1489 653
EcoRV GAT'ATC
Cuts at: 0 2806 5766
Size: 2806 2960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 29 847 1528 1741 2191 5766
Size: 29 818 681 213 450 3575
Fragments arranged by size:
3575 818 681 450 213 29
FspI TGC'GCA
Cuts at: 0 4013 5766
Size: 4013 1753
FspAI rTGC'GCAy
Cuts at: 0 4013 5766
Size: 4013 1753
HaeII r_GCGC'y
Cuts at: 0 3034 5109 5766
Size: 3034 2075 657
Fragments arranged by size:
3034 2075 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1862 1894 2962 2994 3298 3330 3430 3462
Size: 1862 32 1068 32 304 32 100 32
Cuts at: 3462 4495 4527 5191 5223 5766
Size: 1033 32 664 32 543
Fragments arranged by size:
1862 1068 1033 664 543 304 100 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 300 4611 5220 5766
Size: 300 4311 609 546
Fragments arranged by size:
4311 609 546 300
MfeI C'AATT_G
Cuts at: 0 3377 5766
Size: 3377 2389
MscI TGG'CCA
Cuts at: 0 4879 5766
Size: 4879 887
MslI CAynn'nnrTG
Cuts at: 0 3138 3404 4108 4437 4938 4958 5159 5458
Size: 3138 266 704 329 501 20 201 299
Cuts at: 5458 5766
Size: 308
Fragments arranged by size:
3138 704 501 329 308 299 266 201
20
MspA1I CmG'CkG
Cuts at: 0 334 3346 4718 5172 5766
Size: 334 3012 1372 454 594
Fragments arranged by size:
3012 1372 594 454 334
NcoI C'CATG_G
Cuts at: 0 91 5420 5766
Size: 91 5329 346
Fragments arranged by size:
5329 346 91
NdeI CA'TA_TG
Cuts at: 0 1167 1839 4434 5766
Size: 1167 672 2595 1332
Fragments arranged by size:
2595 1332 1167 672
NsiI A_TGCA'T
Cuts at: 0 393 5766
Size: 393 5373
NspI r_CATG'y
Cuts at: 0 1069 3976 4221 5068 5766
Size: 1069 2907 245 847 698
Fragments arranged by size:
2907 1069 847 698 245
PfoI T'CCnGG_A
Cuts at: 0 2208 3879 4325 5766
Size: 2208 1671 446 1441
Fragments arranged by size:
2208 1671 1441 446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 3408 3440 5766
Size: 184 32 3192 32 2326
Fragments arranged by size:
3192 2326 184 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 73 940 5190 5766
Size: 73 867 4250 576
Fragments arranged by size:
4250 867 576 73
PsiI TTA'TAA
Cuts at: 0 2915 5766
Size: 2915 2851
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 917 949 5766
Size: 917 32 4817
Fragments arranged by size:
4817 917 32
PstI C_TGCA'G
Cuts at: 0 44 3083 3201 3460 5709 5766
Size: 44 3039 118 259 2249 57
Fragments arranged by size:
3039 2249 259 118 57 44
PvuII CAG'CTG
Cuts at: 0 334 3346 4718 5766
Size: 334 3012 1372 1048
Fragments arranged by size:
3012 1372 1048 334
SacI G_AGCT'C
Cuts at: 0 5294 5766
Size: 5294 472
SacII CC_GC'GG
Cuts at: 0 5173 5766
Size: 5173 593
SanDI GG'GwC_CC
Cuts at: 0 940 5766
Size: 940 4826
SapI GCTCTTCn'nnn_
Cuts at: 0 1073 1647 4896 5299 5712 5766
Size: 1073 574 3249 403 413 54
Fragments arranged by size:
3249 1073 574 413 403 54
ScaI AGT'ACT
Cuts at: 0 224 5269 5766
Size: 224 5045 497
Fragments arranged by size:
5045 497 224
SexAI A'CCwGG_T
Cuts at: 0 311 434 716 2217 5766
Size: 311 123 282 1501 3549
Fragments arranged by size:
3549 1501 311 282 123
SfcI C'TryA_G
Cuts at: 0 40 985 2606 3079 3197 3456 4506 5022
Size: 40 945 1621 473 118 259 1050 516
Cuts at: 5022 5705 5766
Size: 683 61
Fragments arranged by size:
1621 1050 945 683 516 473 259 118
61 40
SmaI CCC'GGG
Cuts at: 0 5186 5766
Size: 5186 580
SmlI C'TyrA_G
Cuts at: 0 355 688 1755 2527 3811 3858 5329 5766
Size: 355 333 1067 772 1284 47 1471 437
Fragments arranged by size:
1471 1284 1067 772 437 355 333 47
SphI G_CATG'C
Cuts at: 0 3976 4221 5766
Size: 3976 245 1545
Fragments arranged by size:
3976 1545 245
SspI AAT'ATT
Cuts at: 0 2911 4118 5766
Size: 2911 1207 1648
Fragments arranged by size:
2911 1648 1207
StuI AGG'CCT
Cuts at: 0 38 1851 5766
Size: 38 1813 3915
Fragments arranged by size:
3915 1813 38
StyI C'CwwG_G
Cuts at: 0 91 3591 4074 5420 5766
Size: 91 3500 483 1346 346
Fragments arranged by size:
3500 1346 483 346 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1680 5766
Size: 1680 4086
TatI w'GTAC_w
Cuts at: 0 222 861 2453 2731 2849 2924 3703 5267
Size: 222 639 1592 278 118 75 779 1564
Cuts at: 5267 5498 5766
Size: 231 268
Fragments arranged by size:
1592 1564 779 639 278 268 231 222
118 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3752 3784 4510 4542 5766
Size: 3752 32 726 32 1224
Fragments arranged by size:
3752 1224 726 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3906 5766
Size: 3906 1860
XbaI T'CTAG_A
Cuts at: 0 3426 5766
Size: 3426 2340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3137 4104 5766
Size: 3137 967 1662
Fragments arranged by size:
3137 1662 967
XmaI C'CCGG_G
Cuts at: 0 5184 5766
Size: 5184 582
XmnI GAAnn'nnTTC
Cuts at: 0 107 345 665 5355 5766
Size: 107 238 320 4690 411
Fragments arranged by size:
4690 411 320 238 107
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AhdI AlfI
AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
MfeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PfoI PpiI PpuMI PsiI PsrI PstI PvuII SacI
SacII SanDI SapI ScaI SexAI SfcI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AflIII AgeI AleI AloI ApaI ApaLI
AscI AsiSI AvrII BaeI BbeI BglI BmgBI BmtI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI
BssHII BstAPI CspCI EagI FseI HindIII HpaI KasI
KpnI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PshAI
PspOMI PspXI PvuI RsrII SalI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SwaI XhoI ZraI