Restriction map for
pFN21AA0564
(Linear) (Six-base) MAPSORT of: KIAA0564.seq Check: 9314 from: 1 to: 5715
KIAA0564.orf
With 182 enzymes: SgfI *
September 16, 2010 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 72 2396 5715
Size: 72 2324 3319
Fragments arranged by size:
3319 2324 72
AccI GT'mk_AC
Cuts at: 0 4429 4444 5715
Size: 4429 15 1271
Fragments arranged by size:
4429 1271 15
Acc65I G'GTAC_C
Cuts at: 0 2749 5715
Size: 2749 2966
AclI AA'CG_TT
Cuts at: 0 3136 5715
Size: 3136 2579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1710 2176 2437 2839 2996 3314 5386 5596
Size: 1710 466 261 402 157 318 2072 210
Cuts at: 5596 5715
Size: 119
Fragments arranged by size:
2072 1710 466 402 318 261 210 157
119
AfeI AGC'GCT
Cuts at: 0 1081 5715
Size: 1081 4634
AflII C'TTAA_G
Cuts at: 0 823 5715
Size: 823 4892
AflIII A'CryG_T
Cuts at: 0 2569 5411 5715
Size: 2569 2842 304
Fragments arranged by size:
2842 2569 304
AhdI GACnn_n'nnGTC
Cuts at: 0 2978 3158 5715
Size: 2978 180 2557
Fragments arranged by size:
2978 2557 180
AleI CACnn'nnGTG
Cuts at: 0 1776 5715
Size: 1776 3939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 85 5715
Size: 85 5630
AlwNI CAG_nnn'CTG
Cuts at: 0 1782 4936 5715
Size: 1782 3154 779
Fragments arranged by size:
3154 1782 779
ApaI G_GGCC'C
Cuts at: 0 3603 5715
Size: 3603 2112
ApaLI G'TGCA_C
Cuts at: 0 2210 4951 5715
Size: 2210 2741 764
Fragments arranged by size:
2741 2210 764
ApoI r'AATT_y
Cuts at: 0 203 702 1792 1991 2637 2965 3697 4001
Size: 203 499 1090 199 646 328 732 304
Cuts at: 4001 4073 5360 5715
Size: 72 1287 355
Fragments arranged by size:
1287 1090 732 646 499 355 328 304
203 199 72
AseI AT'TA_AT
Cuts at: 0 3332 5715
Size: 3332 2383
AvaI C'yCGr_G
Cuts at: 0 22 147 2402 2809 5715
Size: 22 125 2255 407 2906
Fragments arranged by size:
2906 2255 407 125 22
AvrII C'CTAG_G
Cuts at: 0 2730 5715
Size: 2730 2985
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2420 2453 3235 3268 5715
Size: 2420 33 782 33 2447
Fragments arranged by size:
2447 2420 782 33 33
BamHI G'GATC_C
Cuts at: 0 1938 5715
Size: 1938 3777
BanI G'GyrC_C
Cuts at: 0 27 1212 2749 3494 3513 5715
Size: 27 1185 1537 745 19 2202
Fragments arranged by size:
2202 1537 1185 745 27 19
BanII G_rGCy'C
Cuts at: 0 2810 2849 3603 5715
Size: 2810 39 754 2112
Fragments arranged by size:
2810 2112 754 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 1646 2495 2628 5374 5715
Size: 1646 849 133 2746 341
Fragments arranged by size:
2746 1646 849 341 133
BbvCI CC'TCA_GC
Cuts at: 0 2884 5715
Size: 2884 2831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 416 5715
Size: 382 34 5299
Fragments arranged by size:
5299 382 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 198 1387 2024 2421 3065 3263 5646 5715
Size: 198 1189 637 397 644 198 2383 69
Fragments arranged by size:
2383 1189 644 637 397 198 198 69
BclI T'GATC_A
Cuts at: 0 477 4011 5595 5715
Size: 477 3534 1584 120
Fragments arranged by size:
3534 1584 477 120
BfrBI ATG'CAT
Cuts at: 0 5376 5715
Size: 5376 339
BglI GCCn_nnn'nGGC
Cuts at: 0 44 4745 5715
Size: 44 4701 970
Fragments arranged by size:
4701 970 44
BglII A'GATC_T
Cuts at: 0 4122 5684 5715
Size: 4122 1562 31
Fragments arranged by size:
4122 1562 31
BlpI GC'TnA_GC
Cuts at: 0 626 1606 5715
Size: 626 980 4109
Fragments arranged by size:
4109 980 626
Bme1580I G_kGCm'C
Cuts at: 0 30 1567 2214 3603 4955 5715
Size: 30 1537 647 1389 1352 760
Fragments arranged by size:
1537 1389 1352 760 647 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 2066 3440 4130 5715
Size: 2066 1374 690 1585
Fragments arranged by size:
2066 1585 1374 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2970 5048 5087 5122 5312 5715
Size: 2970 2078 39 35 190 403
Fragments arranged by size:
2970 2078 403 190 39 35
Bpu10I CC'TnA_GC
Cuts at: 0 2843 2884 5715
Size: 2843 41 2831
Fragments arranged by size:
2843 2831 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3790 5224 5505 5540 5715
Size: 3790 1434 281 35 175
Fragments arranged by size:
3790 1434 281 175 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 2986 4152 4800 5543 5715
Size: 2986 1166 648 743 172
Fragments arranged by size:
2986 1166 743 648 172
BsaAI yAC'GTr
Cuts at: 0 2570 4689 5715
Size: 2570 2119 1026
Fragments arranged by size:
2570 2119 1026
BsaBI GATnn'nnATC
Cuts at: 0 994 3076 4265 4413 4615 5495 5715
Size: 994 2082 1189 148 202 880 220
Fragments arranged by size:
2082 1189 994 880 220 202 148
BsaWI w'CCGG_w
Cuts at: 0 1181 5715
Size: 1181 4534
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 836 866 5209 5239 5715
Size: 836 30 4343 30 476
Fragments arranged by size:
4343 836 476 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 1678 2901 3955 4325 5272 5715
Size: 10 1668 1223 1054 370 947 443
Fragments arranged by size:
1668 1223 1054 947 443 370 10
BseYI C'CCAG_C
Cuts at: 0 1782 3519 3949 5715
Size: 1782 1737 430 1766
Fragments arranged by size:
1782 1766 1737 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 476 506 515 3121 3872 5715
Size: 109 367 30 9 2606 751 1843
Fragments arranged by size:
2606 1843 751 367 109 30 9
BsiEI CG_ry'CG
Cuts at: 0 121 5715
Size: 121 5594
BsiHKAI G_wGCw'C
Cuts at: 0 2214 2810 4955 5715
Size: 2214 596 2145 760
Fragments arranged by size:
2214 2145 760 596
BsmI GAATG_Cn'
Cuts at: 0 1512 1544 1563 4864 5715
Size: 1512 32 19 3301 851
Fragments arranged by size:
3301 1512 851 32 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3616 5715
Size: 3616 2099
Bsp1286I G_dGCh'C
Cuts at: 0 30 1567 2214 2810 2849 3603 4955 5715
Size: 30 1537 647 596 39 754 1352 760
Fragments arranged by size:
1537 1352 760 754 647 596 39 30
BspEI T'CCGG_A
Cuts at: 0 1181 5715
Size: 1181 4534
BspHI T'CATG_A
Cuts at: 0 1044 5715
Size: 1044 4671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 72 2396 5715
Size: 72 2324 3319
Fragments arranged by size:
3319 2324 72
BsrDI GCAATG_nn'
Cuts at: 0 398 1733 2742 3347 5715
Size: 398 1335 1009 605 2368
Fragments arranged by size:
2368 1335 1009 605 398
BsrFI r'CCGG_y
Cuts at: 0 142 4763 5198 5715
Size: 142 4621 435 517
Fragments arranged by size:
4621 517 435 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 493 2641 5715
Size: 87 406 2148 3074
Fragments arranged by size:
3074 2148 406 87
BstBI TT'CG_AA
Cuts at: 0 2997 5715
Size: 2997 2718
BstEII G'GTnAC_C
Cuts at: 0 1200 5715
Size: 1200 4515
BstYI r'GATC_y
Cuts at: 0 297 449 1699 1938 3277 4122 4886 5684
Size: 297 152 1250 239 1339 845 764 798
Cuts at: 5684 5715
Size: 31
Fragments arranged by size:
1339 1250 845 798 764 297 239 152
31
BstZ17I GTA'TAC
Cuts at: 0 4430 5715
Size: 4430 1285
Bsu36I CC'TnA_GG
Cuts at: 0 1389 1883 2754 5715
Size: 1389 494 871 2961
Fragments arranged by size:
2961 1389 871 494
BtgI C'CryG_G
Cuts at: 0 39 5648 5715
Size: 39 5609 67
Fragments arranged by size:
5609 67 39
BtsI GCAGTG_nn'
Cuts at: 0 1478 4465 5715
Size: 1478 2987 1250
Fragments arranged by size:
2987 1478 1250
DraI TTT'AAA
Cuts at: 0 1076 1658 2579 5715
Size: 1076 582 921 3136
Fragments arranged by size:
3136 1076 921 582
DraIII CAC_nnn'GTG
Cuts at: 0 1554 4138 5715
Size: 1554 2584 1577
Fragments arranged by size:
2584 1577 1554
DrdI GACnn_nn'nnGTC
Cuts at: 0 4547 5715
Size: 4547 1168
EaeI y'GGCC_r
Cuts at: 0 118 3594 5196 5715
Size: 118 3476 1602 519
Fragments arranged by size:
3476 1602 519 118
EagI C'GGCC_G
Cuts at: 0 118 5715
Size: 118 5597
EarI CTCTTCn'nnn_
Cuts at: 0 347 773 1690 2652 4150 4719 4820 5715
Size: 347 426 917 962 1498 569 101 895
Fragments arranged by size:
1498 962 917 895 569 426 347 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4727 5715
Size: 4727 988
EcoICRI GAG'CTC
Cuts at: 0 2808 5715
Size: 2808 2907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1710 2176 2437 2839 2970 2996 3314 5048
Size: 1710 466 261 402 131 26 318 1734
Cuts at: 5048 5087 5122 5312 5386 5596 5715
Size: 39 35 190 74 210 119
Fragments arranged by size:
1734 1710 466 402 318 261 210 190
131 119 74 39 35 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 2729 3533 4920 5715
Size: 79 2650 804 1387 795
Fragments arranged by size:
2650 1387 804 795 79
EcoO109I rG'GnC_Cy
Cuts at: 0 328 1869 3600 4668 4756 5715
Size: 328 1541 1731 1068 88 959
Fragments arranged by size:
1731 1541 1068 959 328 88
EcoRI G'AATT_C
Cuts at: 0 1792 4073 5715
Size: 1792 2281 1642
Fragments arranged by size:
2281 1792 1642
EcoRV GAT'ATC
Cuts at: 0 3465 4314 5664 5715
Size: 3465 849 1350 51
Fragments arranged by size:
3465 1350 849 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1259 2071 2491 5715
Size: 1259 812 420 3224
Fragments arranged by size:
3224 1259 812 420
HaeII r_GCGC'y
Cuts at: 0 1083 5715
Size: 1083 4632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 434 466 1293 1325 2924 2956 2964 2996
Size: 434 32 827 32 1599 32 8 32
Cuts at: 2996 3144 3176 4003 4035 4047 4079 4114 4146
Size: 148 32 827 32 12 32 35 32
Cuts at: 4146 4360 4392 4505 4537 4630 4662 5715
Size: 214 32 113 32 93 32 1053
Fragments arranged by size:
1599 1053 827 827 434 214 148 113
93 35 32 32 32 32 32 32
32 32 32 32 32 12 8
HincII GTy'rAC
Cuts at: 0 1240 2373 2943 3119 4445 5715
Size: 1240 1133 570 176 1326 1270
Fragments arranged by size:
1326 1270 1240 1133 570 176
HindIII A'AGCT_T
Cuts at: 0 1166 2860 5715
Size: 1166 1694 2855
Fragments arranged by size:
2855 1694 1166
KpnI G_GTAC'C
Cuts at: 0 2753 5715
Size: 2753 2962
MfeI C'AATT_G
Cuts at: 0 839 3155 4242 5715
Size: 839 2316 1087 1473
Fragments arranged by size:
2316 1473 1087 839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1966 2581 2995 5262 5715
Size: 650 1316 615 414 2267 453
Fragments arranged by size:
2267 1316 650 615 453 414
MslI CAynn'nnrTG
Cuts at: 0 1210 1776 3365 5715
Size: 1210 566 1589 2350
Fragments arranged by size:
2350 1589 1210 566
MspA1I CmG'CkG
Cuts at: 0 117 958 3105 3519 4042 5629 5715
Size: 117 841 2147 414 523 1587 86
Fragments arranged by size:
2147 1587 841 523 414 117 86
NaeI GCC'GGC
Cuts at: 0 144 5200 5715
Size: 144 5056 515
Fragments arranged by size:
5056 515 144
NcoI C'CATG_G
Cuts at: 0 5648 5715
Size: 5648 67
NgoMIV G'CCGG_C
Cuts at: 0 142 5198 5715
Size: 142 5056 517
Fragments arranged by size:
5056 517 142
NsiI A_TGCA'T
Cuts at: 0 5378 5715
Size: 5378 337
NspI r_CATG'y
Cuts at: 0 66 1006 2281 3398 4683 5175 5380 5434
Size: 66 940 1275 1117 1285 492 205 54
Cuts at: 5434 5715
Size: 281
Fragments arranged by size:
1285 1275 1117 940 492 281 205 66
54
PflMI CCAn_nnn'nTGG
Cuts at: 0 1782 5113 5715
Size: 1782 3331 602
Fragments arranged by size:
3331 1782 602
PfoI T'CCnGG_A
Cuts at: 0 4970 5715
Size: 4970 745
PmlI CAC'GTG
Cuts at: 0 2570 4689 5715
Size: 2570 2119 1026
Fragments arranged by size:
2570 2119 1026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5248 5280 5715
Size: 5248 32 435
Fragments arranged by size:
5248 435 32
PpuMI rG'GwC_Cy
Cuts at: 0 328 1869 4668 5715
Size: 328 1541 2799 1047
Fragments arranged by size:
2799 1541 1047 328
PsiI TTA'TAA
Cuts at: 0 3238 3683 5715
Size: 3238 445 2032
Fragments arranged by size:
3238 2032 445
PspOMI G'GGCC_C
Cuts at: 0 3599 5715
Size: 3599 2116
PstI C_TGCA'G
Cuts at: 0 4556 5715
Size: 4556 1159
PvuII CAG'CTG
Cuts at: 0 958 3105 4042 5629 5715
Size: 958 2147 937 1587 86
Fragments arranged by size:
2147 1587 958 937 86
SacI G_AGCT'C
Cuts at: 0 2810 5715
Size: 2810 2905
SalI G'TCGA_C
Cuts at: 0 4443 5715
Size: 4443 1272
ScaI AGT'ACT
Cuts at: 0 367 5470 5715
Size: 367 5103 245
Fragments arranged by size:
5103 367 245
SexAI A'CCwGG_T
Cuts at: 0 4790 5715
Size: 4790 925
SfcI C'TryA_G
Cuts at: 0 263 1288 1447 1690 4203 4552 5715
Size: 263 1025 159 243 2513 349 1163
Fragments arranged by size:
2513 1163 1025 349 263 243 159
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 44 5715
Size: 44 5671
SmlI C'TyrA_G
Cuts at: 0 823 2809 3805 5203 5484 5555 5715
Size: 823 1986 996 1398 281 71 160
Fragments arranged by size:
1986 1398 996 823 281 160 71
SphI G_CATG'C
Cuts at: 0 66 5380 5715
Size: 66 5314 335
Fragments arranged by size:
5314 335 66
SspI AAT'ATT
Cuts at: 0 177 5715
Size: 177 5538
StuI AGG'CCT
Cuts at: 0 4739 5715
Size: 4739 976
StyI C'CwwG_G
Cuts at: 0 719 736 1739 2316 2730 2901 5336 5648
Size: 719 17 1003 577 414 171 2435 312
Cuts at: 5648 5657 5715
Size: 9 58
Fragments arranged by size:
2435 1003 719 577 414 312 171 58
17 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4149 4732 5624 5715
Size: 4149 583 892 91
Fragments arranged by size:
4149 892 583 91
TatI w'GTAC_w
Cuts at: 0 365 2436 3413 5468 5715
Size: 365 2071 977 2055 247
Fragments arranged by size:
2071 2055 977 365 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1346 1378 2822 2854 5209 5241 5715
Size: 1346 32 1444 32 2355 32 474
Fragments arranged by size:
2355 1444 1346 474 32 32 32
XbaI T'CTAG_A
Cuts at: 0 3765 5715
Size: 3765 1950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 666 3102 5715
Size: 666 2436 2613
Fragments arranged by size:
2613 2436 666
XhoI C'TCGA_G
Cuts at: 0 2809 5715
Size: 2809 2906
XmnI GAAnn'nnTTC
Cuts at: 0 2027 2411 2606 3649 5715
Size: 2027 384 195 1043 2066
Fragments arranged by size:
2066 2027 1043 384 195
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KpnI MfeI MmeI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PflMI PfoI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuII SacI SalI ScaI SexAI SfcI
SfiI SmlI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AgeI AloI AscI AsiSI BbeI BmgBI BmtI
BplI BsaHI BsiWI BsrBI BsrGI BssHII BssSI BstXI
BtgZI ClaI CspCI FseI FspI FspAI HpaI KasI
MluI MscI NarI NdeI NheI NotI NruI PacI
PasI PciI PmeI PshAI PspXI PsrI PvuI RsrII
SacII SanDI SapI SbfI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI Tth111I XmaI ZraI