Restriction map for
pFN21AA0300
(Linear) (Six-base) MAPSORT of: KIAA0300.seq Check: 9881 from: 1 to: 8517
KIAA0300.orf
With 182 enzymes: SgfI *
December 26, 2011 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 41 2796 8517
Size: 41 2755 5721
Fragments arranged by size:
5721 2755 41
AatII G_ACGT'C
Cuts at: 0 3041 8517
Size: 3041 5476
AccI GT'mk_AC
Cuts at: 0 1687 3761 8517
Size: 1687 2074 4756
Fragments arranged by size:
4756 2074 1687
Acc65I G'GTAC_C
Cuts at: 0 2533 8517
Size: 2533 5984
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 4718 4955 5262 5674 6311 8083 8281
Size: 713 4005 237 307 412 637 1772 198
Cuts at: 8281 8517
Size: 236
Fragments arranged by size:
4005 1772 713 637 412 307 237 236
198
AfeI AGC'GCT
Cuts at: 0 711 1066 5103 8517
Size: 711 355 4037 3414
Fragments arranged by size:
4037 3414 711 355
AflII C'TTAA_G
Cuts at: 0 1698 8517
Size: 1698 6819
AflIII A'CryG_T
Cuts at: 0 4810 8517
Size: 4810 3707
AhdI GACnn_n'nnGTC
Cuts at: 0 4686 4728 5624 8517
Size: 4686 42 896 2893
Fragments arranged by size:
4686 2893 896 42
AleI CACnn'nnGTG
Cuts at: 0 1093 7154 8517
Size: 1093 6061 1363
Fragments arranged by size:
6061 1363 1093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 7246 8111 8459 8517
Size: 7246 865 348 58
Fragments arranged by size:
7246 865 348 58
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3297 3329 6118 6150 8517
Size: 3297 32 2789 32 2367
Fragments arranged by size:
3297 2789 2367 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 54 123 244 1641 4150 5923 6307 7452
Size: 54 69 121 1397 2509 1773 384 1145
Cuts at: 7452 7616 7698 8517
Size: 164 82 819
Fragments arranged by size:
2509 1773 1397 1145 819 384 164 121
82 69 54
ApaI G_GGCC'C
Cuts at: 0 2162 2964 6616 8327 8517
Size: 2162 802 3652 1711 190
Fragments arranged by size:
3652 2162 1711 802 190
ApoI r'AATT_y
Cuts at: 0 878 1034 4575 7349 8396 8508 8517
Size: 878 156 3541 2774 1047 112 9
Fragments arranged by size:
3541 2774 1047 878 156 112 9
AseI AT'TA_AT
Cuts at: 0 4520 5252 8408 8491 8517
Size: 4520 732 3156 83 26
Fragments arranged by size:
4520 3156 732 83 26
AvaI C'yCGr_G
Cuts at: 0 1607 2670 2967 3000 3468 3873 4230 4245
Size: 1607 1063 297 33 468 405 357 15
Cuts at: 4245 4425 4446 4833 6581 6618 6737 6775 7594
Size: 180 21 387 1748 37 119 38 819
Cuts at: 7594 8517
Size: 923
Fragments arranged by size:
1748 1607 1063 923 819 468 405 387
357 297 180 119 38 37 33 21
15
BamHI G'GATC_C
Cuts at: 0 1586 8517
Size: 1586 6931
BanI G'GyrC_C
Cuts at: 0 1108 2224 2435 2533 3093 4164 4468 6081
Size: 1108 1116 211 98 560 1071 304 1613
Cuts at: 6081 6588 7222 8026 8517
Size: 507 634 804 491
Fragments arranged by size:
1613 1116 1108 1071 804 634 560 507
491 304 211 98
BanII G_rGCy'C
Cuts at: 0 1184 2026 2162 2795 2964 3208 3310 3346
Size: 1184 842 136 633 169 244 102 36
Cuts at: 3346 4062 4267 4405 5017 6616 7093 7312 8327
Size: 716 205 138 612 1599 477 219 1015
Cuts at: 8327 8432 8517
Size: 105 85
Fragments arranged by size:
1599 1184 1015 842 716 633 612 477
244 219 205 169 138 136 105 102
85 36
BbeI G_GCGC'C
Cuts at: 0 6085 8030 8517
Size: 6085 1945 487
Fragments arranged by size:
6085 1945 487
BbsI GAAGACnn'nnnn_
Cuts at: 0 949 1355 1893 2155 4319 4601 6793 7109
Size: 949 406 538 262 2164 282 2192 316
Cuts at: 7109 8269 8517
Size: 1160 248
Fragments arranged by size:
2192 2164 1160 949 538 406 316 282
262 248
BbvCI CC'TCA_GC
Cuts at: 0 370 1445 3398 4664 4878 5555 8517
Size: 370 1075 1953 1266 214 677 2962
Fragments arranged by size:
2962 1953 1266 1075 677 370 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3760 3794 6938 6972 7503 7537 8517
Size: 3760 34 3144 34 531 34 980
Fragments arranged by size:
3760 3144 980 531 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3142 8517
Size: 3142 5375
BclI T'GATC_A
Cuts at: 0 177 518 8517
Size: 177 341 7999
Fragments arranged by size:
7999 341 177
BglI GCCn_nnn'nGGC
Cuts at: 0 685 3091 5672 6457 8035 8517
Size: 685 2406 2581 785 1578 482
Fragments arranged by size:
2581 2406 1578 785 685 482
BglII A'GATC_T
Cuts at: 0 200 1302 8517
Size: 200 1102 7215
Fragments arranged by size:
7215 1102 200
BlpI GC'TnA_GC
Cuts at: 0 485 5109 5662 8043 8517
Size: 485 4624 553 2381 474
Fragments arranged by size:
4624 2381 553 485 474
Bme1580I G_kGCm'C
Cuts at: 0 2162 2964 4167 4238 4473 6593 6616 8327
Size: 2162 802 1203 71 235 2120 23 1711
Cuts at: 8327 8517
Size: 190
Fragments arranged by size:
2162 2120 1711 1203 802 235 190 71
23
BmgBI CAC'GTC
Cuts at: 0 2690 8517
Size: 2690 5827
BmrI ACTGGGnnnn_n'
Cuts at: 0 2592 3305 3311 3404 5856 6675 7655 8517
Size: 2592 713 6 93 2452 819 980 862
Fragments arranged by size:
2592 2452 980 862 819 713 93 6
BmtI G_CTAG'C
Cuts at: 0 5108 8517
Size: 5108 3409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 444 2570 4048 4859 5031 7518 8517
Size: 444 2126 1478 811 172 2487 999
Fragments arranged by size:
2487 2126 1478 999 811 444 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 769 1826 2267 2390 3330 3581 3824
Size: 651 118 1057 441 123 940 251 243
Cuts at: 3824 4004 4046 4156 4412 7429 7561 7598 8224
Size: 180 42 110 256 3017 132 37 626
Cuts at: 8224 8408 8517
Size: 184 109
Fragments arranged by size:
3017 1057 940 651 626 441 256 251
243 184 180 132 123 118 110 109
42 37
Bpu10I CC'TnA_GC
Cuts at: 0 370 1445 2314 2529 3398 4367 4664 4878
Size: 370 1075 869 215 869 969 297 214
Cuts at: 4878 5555 5978 7890 8517
Size: 677 423 1912 627
Fragments arranged by size:
1912 1075 969 869 869 677 627 423
370 297 215 214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1076 1486 1555 2412 4143 5653 6672
Size: 802 274 410 69 857 1731 1510 1019
Cuts at: 6672 7209 7482 8517
Size: 537 273 1035
Fragments arranged by size:
1731 1510 1035 1019 857 802 537 410
274 273 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 1608 3842 4019 4325 7288 8517
Size: 1608 2234 177 306 2963 1229
Fragments arranged by size:
2963 2234 1608 1229 306 177
BsaAI yAC'GTr
Cuts at: 0 4813 8517
Size: 4813 3704
BsaBI GATnn'nnATC
Cuts at: 0 3921 7797 8517
Size: 3921 3876 720
Fragments arranged by size:
3921 3876 720
BsaHI Gr'CG_yC
Cuts at: 0 3038 6082 6502 8027 8517
Size: 3038 3044 420 1525 490
Fragments arranged by size:
3044 3038 1525 490 420
BsaWI w'CCGG_w
Cuts at: 0 1589 1979 8517
Size: 1589 390 6538
Fragments arranged by size:
6538 1589 390
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 446 476 879 909 1613 1643 2837 2867
Size: 446 30 403 30 704 30 1194 30
Cuts at: 2867 3297 3327 4588 4618 5294 5324 5786 5816
Size: 430 30 1261 30 676 30 462 30
Cuts at: 5816 8517
Size: 2701
Fragments arranged by size:
2701 1261 1194 704 676 462 446 430
403 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 887 1264 2710 2857 3139 3684 4431
Size: 31 856 377 1446 147 282 545 747
Cuts at: 4431 4464 4727 5457 5543 6498 6501 6504 8517
Size: 33 263 730 86 955 3 3 2013
Fragments arranged by size:
2013 1446 955 856 747 730 545 377
282 263 147 86 33 31 3 3
BseYI C'CCAG_C
Cuts at: 0 228 384 612 736 1029 2026 2713 3320
Size: 228 156 228 124 293 997 687 607
Cuts at: 3320 3660 3745 3793 4496 4988 5722 6805 8272
Size: 340 85 48 703 492 734 1083 1467
Cuts at: 8272 8277 8517
Size: 5 240
Fragments arranged by size:
1467 1083 997 734 703 687 607 492
340 293 240 228 228 156 124 85
48 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 1252 5096 8056 8071 8503 8517
Size: 13 1239 3844 2960 15 432 14
Fragments arranged by size:
3844 2960 1239 432 15 14 13
BsiEI CG_ry'CG
Cuts at: 0 2433 6246 8517
Size: 2433 3813 2271
Fragments arranged by size:
3813 2433 2271
BsiHKAI G_wGCw'C
Cuts at: 0 3603 4062 4368 4405 5017 7093 7526 8517
Size: 3603 459 306 37 612 2076 433 991
Fragments arranged by size:
3603 2076 991 612 459 433 306 37
BsmI GAATG_Cn'
Cuts at: 0 1820 5715 6602 8517
Size: 1820 3895 887 1915
Fragments arranged by size:
3895 1915 1820 887
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4643 6253 6901 7173 8517
Size: 4643 1610 648 272 1344
Fragments arranged by size:
4643 1610 1344 648 272
Bsp1286I G_dGCh'C
Cuts at: 0 1184 2026 2162 2795 2964 3208 3310 3346
Size: 1184 842 136 633 169 244 102 36
Cuts at: 3346 3603 4062 4167 4238 4267 4368 4405 4473
Size: 257 459 105 71 29 101 37 68
Cuts at: 4473 5017 6593 6616 7093 7312 7526 8327 8432
Size: 544 1576 23 477 219 214 801 105
Cuts at: 8432 8517
Size: 85
Fragments arranged by size:
1576 1184 842 801 633 544 477 459
257 244 219 214 169 136 105 105
102 101 85 71 68 37 36 29
23
BspEI T'CCGG_A
Cuts at: 0 1589 1979 8517
Size: 1589 390 6538
Fragments arranged by size:
6538 1589 390
BspHI T'CATG_A
Cuts at: 0 4590 7259 8517
Size: 4590 2669 1258
Fragments arranged by size:
4590 2669 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 2796 3647 5219 5987 8517
Size: 41 2755 851 1572 768 2530
Fragments arranged by size:
2755 2530 1572 851 768 41
BsrBI CCG'CTC
Cuts at: 0 1114 1694 3733 3976 7436 8517
Size: 1114 580 2039 243 3460 1081
Fragments arranged by size:
3460 2039 1114 1081 580 243
BsrDI GCAATG_nn'
Cuts at: 0 918 1412 8517
Size: 918 494 7105
Fragments arranged by size:
7105 918 494
BsrFI r'CCGG_y
Cuts at: 0 2432 2647 6102 7046 7219 8517
Size: 2432 215 3455 944 173 1298
Fragments arranged by size:
3455 2432 1298 944 215 173
BsrGI T'GTAC_A
Cuts at: 0 1524 1766 8232 8345 8517
Size: 1524 242 6466 113 172
Fragments arranged by size:
6466 1524 242 172 113
BssSI C'ACGA_G
Cuts at: 0 1189 2761 4706 5070 5818 5928 8101 8517
Size: 1189 1572 1945 364 748 110 2173 416
Fragments arranged by size:
2173 1945 1572 1189 748 416 364 110
BstEII G'GTnAC_C
Cuts at: 0 616 4066 6709 7559 8517
Size: 616 3450 2643 850 958
Fragments arranged by size:
3450 2643 958 850 616
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 480 1227 3033 3957 6180 8202 8517
Size: 228 252 747 1806 924 2223 2022 315
Fragments arranged by size:
2223 2022 1806 924 747 315 252 228
BstYI r'GATC_y
Cuts at: 0 200 422 1302 1586 2065 3388 4767 7695
Size: 200 222 880 284 479 1323 1379 2928
Cuts at: 7695 8517
Size: 822
Fragments arranged by size:
2928 1379 1323 880 822 479 284 222
200
Bsu36I CC'TnA_GG
Cuts at: 0 1137 1276 3967 8517
Size: 1137 139 2691 4550
Fragments arranged by size:
4550 2691 1137 139
BtgI C'CryG_G
Cuts at: 0 1220 2319 5114 6137 8049 8517
Size: 1220 1099 2795 1023 1912 468
Fragments arranged by size:
2795 1912 1220 1099 1023 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3791 4673 8517
Size: 3791 882 3844
Fragments arranged by size:
3844 3791 882
BtsI GCAGTG_nn'
Cuts at: 0 496 1205 3378 4236 4368 5795 6302 6771
Size: 496 709 2173 858 132 1427 507 469
Cuts at: 6771 8517
Size: 1746
Fragments arranged by size:
2173 1746 1427 858 709 507 496 469
132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2073 2108 3393 3428 5971 6006 8517
Size: 2073 35 1285 35 2543 35 2511
Fragments arranged by size:
2543 2511 2073 1285 35 35 35
DraI TTT'AAA
Cuts at: 0 5406 8517
Size: 5406 3111
DraIII CAC_nnn'GTG
Cuts at: 0 1677 7956 8517
Size: 1677 6279 561
Fragments arranged by size:
6279 1677 561
DrdI GACnn_nn'nnGTC
Cuts at: 0 2173 2828 3472 6707 6748 8517
Size: 2173 655 644 3235 41 1769
Fragments arranged by size:
3235 2173 1769 655 644 41
EaeI y'GGCC_r
Cuts at: 0 110 780 987 1202 1244 1614 1738 2294
Size: 110 670 207 215 42 370 124 556
Cuts at: 2294 2430 2645 3362 3781 4221 4925 6170 6236
Size: 136 215 717 419 440 704 1245 66
Cuts at: 6236 7034 7241 8517
Size: 798 207 1276
Fragments arranged by size:
1276 1245 798 717 704 670 556 440
419 370 215 215 207 207 136 124
110 66 42
EagI C'GGCC_G
Cuts at: 0 2430 8517
Size: 2430 6087
EarI CTCTTCn'nnn_
Cuts at: 0 307 388 1364 1395 1653 2539 2800 2915
Size: 307 81 976 31 258 886 261 115
Cuts at: 2915 3027 3104 3714 4778 4989 5590 5650 6373
Size: 112 77 610 1064 211 601 60 723
Cuts at: 6373 7531 8517
Size: 1158 986
Fragments arranged by size:
1158 1064 986 976 886 723 610 601
307 261 258 211 115 112 81 77
60 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1206 2058 2347 2860 5150 8517
Size: 1206 852 289 513 2290 3367
Fragments arranged by size:
3367 2290 1206 852 513 289
EcoICRI GAG'CTC
Cuts at: 0 4060 4403 5015 7091 8517
Size: 4060 343 612 2076 1426
Fragments arranged by size:
4060 2076 1426 612 343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 713 769 1826 2267 2390 3330 3581
Size: 651 62 56 1057 441 123 940 251
Cuts at: 3581 3824 4004 4046 4156 4412 4718 4955 5262
Size: 243 180 42 110 256 306 237 307
Cuts at: 5262 5674 6311 7429 7561 7598 8083 8224 8281
Size: 412 637 1118 132 37 485 141 57
Cuts at: 8281 8408 8517
Size: 127 109
Fragments arranged by size:
1118 1057 940 651 637 485 441 412
307 306 256 251 243 237 180 141
132 127 123 110 109 62 57 56
42 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 2528 4135 4446 5981 7577 8517
Size: 220 2308 1607 311 1535 1596 940
Fragments arranged by size:
2308 1607 1596 1535 940 311 220
EcoO109I rG'GnC_Cy
Cuts at: 0 331 687 1269 1578 1785 2410 2850 2960
Size: 331 356 582 309 207 625 440 110
Cuts at: 2960 2961 2971 3192 3979 4454 5066 5583 5697
Size: 1 10 221 787 475 612 517 114
Cuts at: 5697 5823 5855 6612 6613 6662 6750 7613 8135
Size: 126 32 757 1 49 88 863 522
Cuts at: 8135 8324 8476 8517
Size: 189 152 41
Fragments arranged by size:
863 787 757 625 612 582 522 517
475 440 356 331 309 221 207 189
152 126 114 110 88 49 41 32
10 1 1
EcoRI G'AATT_C
Cuts at: 0 878 1034 7349 8508 8517
Size: 878 156 6315 1159 9
Fragments arranged by size:
6315 1159 878 156 9
EcoRV GAT'ATC
Cuts at: 0 6891 8517
Size: 6891 1626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 966 1479 1872 8517
Size: 966 513 393 6645
Fragments arranged by size:
6645 966 513 393
HaeII r_GCGC'y
Cuts at: 0 713 1068 5105 6085 7520 8030 8517
Size: 713 355 4037 980 1435 510 487
Fragments arranged by size:
4037 1435 980 713 510 487 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 446 478 879 911 940 972 1315 1347
Size: 446 32 401 32 29 32 343 32
Cuts at: 1347 3142 3174 3451 3483 3523 3555 3679 3691
Size: 1795 32 277 32 40 32 124 12
Cuts at: 3691 3711 3723 4138 4170 4333 4365 4588 4620
Size: 20 12 415 32 163 32 223 32
Cuts at: 4620 4672 4704 4714 4746 5001 5033 5610 5642
Size: 52 32 10 32 255 32 577 32
Cuts at: 5642 7260 7292 7921 7953 8517
Size: 1618 32 629 32 564
Fragments arranged by size:
1795 1618 629 577 564 446 415 401
343 277 255 223 163 124 52 40
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
29 20 12 12 10
HincII GTy'rAC
Cuts at: 0 1283 2352 5688 8022 8517
Size: 1283 1069 3336 2334 495
Fragments arranged by size:
3336 2334 1283 1069 495
HpaI GTT'AAC
Cuts at: 0 1283 8517
Size: 1283 7234
KasI G'GCGC_C
Cuts at: 0 6081 8026 8517
Size: 6081 1945 491
Fragments arranged by size:
6081 1945 491
KpnI G_GTAC'C
Cuts at: 0 2537 8517
Size: 2537 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1577 2125 2190 2672 3316 8517
Size: 432 1145 548 65 482 644 5201
Fragments arranged by size:
5201 1145 644 548 482 432 65
MscI TGG'CCA
Cuts at: 0 1204 1246 2296 3364 4223 4927 6172 6238
Size: 1204 42 1050 1068 859 704 1245 66
Cuts at: 6238 7036 7243 8517
Size: 798 207 1274
Fragments arranged by size:
1274 1245 1204 1068 1050 859 798 704
207 66 42
MslI CAynn'nnrTG
Cuts at: 0 358 1093 1121 1225 2056 3614 6136 7154
Size: 358 735 28 104 831 1558 2522 1018
Cuts at: 7154 8054 8093 8441 8517
Size: 900 39 348 76
Fragments arranged by size:
2522 1558 1018 900 831 735 358 348
104 76 39 28
MspA1I CmG'CkG
Cuts at: 0 96 141 444 1638 1873 2321 3584 3889
Size: 96 45 303 1194 235 448 1263 305
Cuts at: 3889 4006 5130 5260 5799 5845 6267 6328 6846
Size: 117 1124 130 539 46 422 61 518
Cuts at: 6846 7011 7750 8085 8517
Size: 165 739 335 432
Fragments arranged by size:
1263 1194 1124 739 539 518 448 432
422 335 305 303 235 165 130 117
96 61 46 45
NaeI GCC'GGC
Cuts at: 0 2434 7221 8517
Size: 2434 4787 1296
Fragments arranged by size:
4787 2434 1296
NarI GG'CG_CC
Cuts at: 0 6082 8027 8517
Size: 6082 1945 490
Fragments arranged by size:
6082 1945 490
NcoI C'CATG_G
Cuts at: 0 5114 8517
Size: 5114 3403
NgoMIV G'CCGG_C
Cuts at: 0 2432 7219 8517
Size: 2432 4787 1298
Fragments arranged by size:
4787 2432 1298
NheI G'CTAG_C
Cuts at: 0 5104 8517
Size: 5104 3413
NspI r_CATG'y
Cuts at: 0 4220 5991 6135 7470 8517
Size: 4220 1771 144 1335 1047
Fragments arranged by size:
4220 1771 1335 1047 144
PasI CC'CwG_GG
Cuts at: 0 2139 2155 2956 3715 3812 6360 6552 8517
Size: 2139 16 801 759 97 2548 192 1965
Fragments arranged by size:
2548 2139 1965 801 759 192 97 16
PflMI CCAn_nnn'nTGG
Cuts at: 0 2209 8517
Size: 2209 6308
PfoI T'CCnGG_A
Cuts at: 0 1333 2283 2333 2406 6902 7135 7445 7620
Size: 1333 950 50 73 4496 233 310 175
Cuts at: 7620 8517
Size: 897
Fragments arranged by size:
4496 1333 950 897 310 233 175 73
50
PmlI CAC'GTG
Cuts at: 0 4813 8517
Size: 4813 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3297 3329 7384 7416 8517
Size: 3297 32 4055 32 1101
Fragments arranged by size:
4055 3297 1101 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 331 1269 1578 2410 2971 3979 5583 5823
Size: 331 938 309 832 561 1008 1604 240
Cuts at: 5823 5855 6662 6750 8476 8517
Size: 32 807 88 1726 41
Fragments arranged by size:
1726 1604 1008 938 832 807 561 331
309 240 88 41 32
PshAI GACnn'nnGTC
Cuts at: 0 5944 8517
Size: 5944 2573
PsiI TTA'TAA
Cuts at: 0 560 8517
Size: 560 7957
PspOMI G'GGCC_C
Cuts at: 0 2158 2960 6612 8323 8517
Size: 2158 802 3652 1711 194
Fragments arranged by size:
3652 2158 1711 802 194
PspXI vC'TCGA_Gb
Cuts at: 0 4833 8517
Size: 4833 3684
PstI C_TGCA'G
Cuts at: 0 59 71 128 1878 2686 3189 4314 7201
Size: 59 12 57 1750 808 503 1125 2887
Cuts at: 7201 7492 8517
Size: 291 1025
Fragments arranged by size:
2887 1750 1125 1025 808 503 291 59
57 12
PvuI CG_AT'CG
Cuts at: 0 6246 8517
Size: 6246 2271
PvuII CAG'CTG
Cuts at: 0 141 444 1638 1873 3584 4006 5260 5845
Size: 141 303 1194 235 1711 422 1254 585
Cuts at: 5845 6267 6328 8085 8517
Size: 422 61 1757 432
Fragments arranged by size:
1757 1711 1254 1194 585 432 422 422
303 235 141 61
SacI G_AGCT'C
Cuts at: 0 4062 4405 5017 7093 8517
Size: 4062 343 612 2076 1424
Fragments arranged by size:
4062 2076 1424 612 343
SacII CC_GC'GG
Cuts at: 0 2322 8517
Size: 2322 6195
SanDI GG'GwC_CC
Cuts at: 0 1578 2971 8517
Size: 1578 1393 5546
Fragments arranged by size:
5546 1578 1393
SapI GCTCTTCn'nnn_
Cuts at: 0 1395 2800 7531 8517
Size: 1395 1405 4731 986
Fragments arranged by size:
4731 1405 1395 986
SbfI CC_TGCA'GG
Cuts at: 0 71 8517
Size: 71 8446
ScaI AGT'ACT
Cuts at: 0 2602 8517
Size: 2602 5915
SfcI C'TryA_G
Cuts at: 0 55 67 124 1874 2682 3185 3758 3903
Size: 55 12 57 1750 808 503 573 145
Cuts at: 3903 4310 5359 7052 7197 7488 8517
Size: 407 1049 1693 145 291 1029
Fragments arranged by size:
1750 1693 1049 1029 808 573 503 407
291 145 145 57 55 12
SfoI GGC'GCC
Cuts at: 0 6083 8028 8517
Size: 6083 1945 489
Fragments arranged by size:
6083 1945 489
SmaI CCC'GGG
Cuts at: 0 2672 2969 3875 4247 4427 8517
Size: 2672 297 906 372 180 4090
Fragments arranged by size:
4090 2672 906 372 297 180
SmlI C'TyrA_G
Cuts at: 0 817 1091 1501 1534 1698 2391 3468 4158
Size: 817 274 410 33 164 693 1077 690
Cuts at: 4158 4833 5668 6687 7188 7461 8517
Size: 675 835 1019 501 273 1056
Fragments arranged by size:
1077 1056 1019 835 817 693 690 675
501 410 274 273 164 33
SphI G_CATG'C
Cuts at: 0 7470 8517
Size: 7470 1047
SspI AAT'ATT
Cuts at: 0 8454 8517
Size: 8454 63
StuI AGG'CCT
Cuts at: 0 672 2982 3338 4616 8517
Size: 672 2310 356 1278 3901
Fragments arranged by size:
3901 2310 1278 672 356
StyI C'CwwG_G
Cuts at: 0 221 605 701 1454 1723 1788 2162 3026
Size: 221 384 96 753 269 65 374 864
Cuts at: 3026 3197 5114 5427 6646 8327 8517
Size: 171 1917 313 1219 1681 190
Fragments arranged by size:
1917 1681 1219 864 753 384 374 313
269 221 190 171 96 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 348 604 635 2064 3267 3298 4391
Size: 25 323 256 31 1429 1203 31 1093
Cuts at: 4391 4609 4870 7166 7674 8517
Size: 218 261 2296 508 843
Fragments arranged by size:
2296 1429 1203 1093 843 508 323 261
256 218 31 31 25
TatI w'GTAC_w
Cuts at: 0 131 205 1524 1766 2600 8232 8345 8517
Size: 131 74 1319 242 834 5632 113 172
Fragments arranged by size:
5632 1319 834 242 172 131 113 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2837 2869 3273 3305 5786 5818 6411 6443
Size: 2837 32 404 32 2481 32 593 32
Cuts at: 6443 7892 7924 8517
Size: 1449 32 593
Fragments arranged by size:
2837 2481 1449 593 593 404 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 8285 8517
Size: 8285 232
XbaI T'CTAG_A
Cuts at: 0 840 4570 8517
Size: 840 3730 3947
Fragments arranged by size:
3947 3730 840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 744 1461 2617 2785 3681 5502 8517
Size: 744 717 1156 168 896 1821 3015
Fragments arranged by size:
3015 1821 1156 896 744 717 168
XhoI C'TCGA_G
Cuts at: 0 3468 4833 8517
Size: 3468 1365 3684
Fragments arranged by size:
3684 3468 1365
XmaI C'CCGG_G
Cuts at: 0 2670 2967 3873 4245 4425 8517
Size: 2670 297 906 372 180 4092
Fragments arranged by size:
4092 2670 906 372 297 180
XmnI GAAnn'nnTTC
Cuts at: 0 1958 5612 8517
Size: 1958 3654 2905
Fragments arranged by size:
3654 2905 1958
ZraI GAC'GTC
Cuts at: 0 3039 8517
Size: 3039 5478
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HpaI KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SanDI SapI SbfI ScaI SfcI SfoI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AgeI ApaLI AscI AsiSI AvrII BaeI BfrBI
BsiWI BssHII BstAPI BstBI BstZ17I ClaI FseI FspI
FspAI HindIII MfeI MluI NdeI NotI NruI NsiI
PacI PciI PmeI PsrI RsrII SalI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SwaI