Restriction map for
pFN21AA0002
(Linear) (Six-base) MAPSORT of: KIAA0002.seq Check: 4637 from: 1 to: 1644
KIAA0002.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 0 1175 1644
Size: 1175 469
AclI AA'CG_TT
Cuts at: 0 1430 1644
Size: 1430 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 629 806 1472 1644
Size: 365 264 177 666 172
Fragments arranged by size:
666 365 264 177 172
AflIII A'CryG_T
Cuts at: 0 1285 1644
Size: 1285 359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 260 1644
Size: 260 1384
AlwNI CAG_nnn'CTG
Cuts at: 0 1549 1644
Size: 1549 95
ApaI G_GGCC'C
Cuts at: 0 1598 1644
Size: 1598 46
ApoI r'AATT_y
Cuts at: 0 617 798 1644
Size: 617 181 846
Fragments arranged by size:
846 617 181
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1061 1094 1644
Size: 1061 33 550
Fragments arranged by size:
1061 550 33
BanI G'GyrC_C
Cuts at: 0 1108 1644
Size: 1108 536
BanII G_rGCy'C
Cuts at: 0 330 628 1598 1644
Size: 330 298 970 46
Fragments arranged by size:
970 330 298 46
BbeI G_GCGC'C
Cuts at: 0 1112 1644
Size: 1112 532
BbsI GAAGACnn'nnnn_
Cuts at: 0 972 1644
Size: 972 672
BbvCI CC'TCA_GC
Cuts at: 0 1325 1644
Size: 1325 319
Bme1580I G_kGCm'C
Cuts at: 0 1598 1644
Size: 1598 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1632 1644
Size: 1632 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1388 1644
Size: 344 1044 256
Fragments arranged by size:
1044 344 256
Bpu10I CC'TnA_GC
Cuts at: 0 551 1325 1644
Size: 551 774 319
Fragments arranged by size:
774 551 319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1644
Size: 28 1616
BsaHI Gr'CG_yC
Cuts at: 0 1109 1644
Size: 1109 535
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 1644
Size: 172 30 1442
Fragments arranged by size:
1442 172 30
BsiHKAI G_wGCw'C
Cuts at: 0 330 1644
Size: 330 1314
Bsp1286I G_dGCh'C
Cuts at: 0 330 628 1598 1644
Size: 330 298 970 46
Fragments arranged by size:
970 330 298 46
BspHI T'CATG_A
Cuts at: 0 417 1644
Size: 417 1227
BstYI r'GATC_y
Cuts at: 0 957 1644
Size: 957 687
BtsI GCAGTG_nn'
Cuts at: 0 725 1644
Size: 725 919
EaeI y'GGCC_r
Cuts at: 0 536 582 1644
Size: 536 46 1062
Fragments arranged by size:
1062 536 46
EarI CTCTTCn'nnn_
Cuts at: 0 71 648 908 1001 1644
Size: 71 577 260 93 643
Fragments arranged by size:
643 577 260 93 71
EcoICRI GAG'CTC
Cuts at: 0 328 1644
Size: 328 1316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 365 629 806 1388 1472 1644
Size: 344 21 264 177 582 84 172
Fragments arranged by size:
582 344 264 177 172 84 21
HaeII r_GCGC'y
Cuts at: 0 7 1112 1644
Size: 7 1105 532
Fragments arranged by size:
1105 532 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 679 711 1644
Size: 463 32 184 32 933
Fragments arranged by size:
933 463 184 32 32
HindIII A'AGCT_T
Cuts at: 0 101 541 1644
Size: 101 440 1103
Fragments arranged by size:
1103 440 101
KasI G'GCGC_C
Cuts at: 0 1108 1644
Size: 1108 536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1044 1413 1644
Size: 270 774 369 231
Fragments arranged by size:
774 369 270 231
MscI TGG'CCA
Cuts at: 0 538 1644
Size: 538 1106
MslI CAynn'nnrTG
Cuts at: 0 590 934 1644
Size: 590 344 710
Fragments arranged by size:
710 590 344
MspA1I CmG'CkG
Cuts at: 0 1588 1644
Size: 1588 56
NarI GG'CG_CC
Cuts at: 0 1109 1644
Size: 1109 535
NdeI CA'TA_TG
Cuts at: 0 138 272 1275 1644
Size: 138 134 1003 369
Fragments arranged by size:
1003 369 138 134
NspI r_CATG'y
Cuts at: 0 560 1289 1476 1644
Size: 560 729 187 168
Fragments arranged by size:
729 560 187 168
PciI A'CATG_T
Cuts at: 0 1285 1644
Size: 1285 359
PflMI CCAn_nnn'nTGG
Cuts at: 0 1591 1644
Size: 1591 53
PfoI T'CCnGG_A
Cuts at: 0 332 1290 1644
Size: 332 958 354
Fragments arranged by size:
958 354 332
PspOMI G'GGCC_C
Cuts at: 0 1594 1644
Size: 1594 50
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 215 247 1644
Size: 215 32 1397
Fragments arranged by size:
1397 215 32
PstI C_TGCA'G
Cuts at: 0 409 1545 1644
Size: 409 1136 99
Fragments arranged by size:
1136 409 99
PvuII CAG'CTG
Cuts at: 0 1588 1644
Size: 1588 56
SacI G_AGCT'C
Cuts at: 0 330 1644
Size: 330 1314
SapI GCTCTTCn'nnn_
Cuts at: 0 908 1001 1644
Size: 908 93 643
Fragments arranged by size:
908 643 93
ScaI AGT'ACT
Cuts at: 0 1127 1644
Size: 1127 517
SfcI C'TryA_G
Cuts at: 0 405 989 1139 1541 1644
Size: 405 584 150 402 103
Fragments arranged by size:
584 405 402 150 103
SfoI GGC'GCC
Cuts at: 0 1110 1644
Size: 1110 534
SmlI C'TyrA_G
Cuts at: 0 43 1644
Size: 43 1601
SphI G_CATG'C
Cuts at: 0 560 1644
Size: 560 1084
SspI AAT'ATT
Cuts at: 0 1513 1644
Size: 1513 131
StyI C'CwwG_G
Cuts at: 0 17 1644
Size: 17 1627
TatI w'GTAC_w
Cuts at: 0 231 722 1125 1407 1551 1644
Size: 231 491 403 282 144 93
Fragments arranged by size:
491 403 282 231 144 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1001 1033 1644
Size: 1001 32 611
Fragments arranged by size:
1001 611 32
XbaI T'CTAG_A
Cuts at: 0 1491 1644
Size: 1491 153
XmnI GAAnn'nnTTC
Cuts at: 0 350 530 1340 1644
Size: 350 180 810 304
Fragments arranged by size:
810 350 304 180
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI ApaI ApoI
BaeI BanI BanII BbeI BbsI BbvCI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaHI BsaXI BsiHKAI Bsp1286I BspHI
BstYI BtsI EaeI EarI EcoICRI Eco57MI HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NarI NdeI
NspI PciI PflMI PfoI PspOMI PsrI PstI PvuII
SacI SapI ScaI SfcI SfoI SmlI SphI SspI
StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaWI BseRI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KpnI MfeI
MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI TaqII Tth111I XcmI XhoI XmaI ZraI