Restriction map for
pF1KE2677
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspMI BsrBI BsrFI BssHII
BstEII BstPI BstUI CelII Cfr10I
ClaI CpoI DraII DsaI EagI
Eam1105I EclXI Eco52I EcoNI EcoO109I
EcoO65I FauI FseI FspI HgaI
Hin1I KasI KpnI KspI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NlaIV NotI NruI PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 561 GTATAC GT^[AC][GT]AC
AciI 1418 CCGC CCGC(-3/-1)
AflIII 2841 ACATGT A^C[GA][CT]GT
AocI 2458 CCTAAGG CC^T[ACGT]AGG
Aor51HI 3441 AGCGCT AGC^GCT
AspHI 5265 GAGCTC G[AT]GC[AT]^C
AspI 428 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1952 CTCGAG C^[CT]CG[GA]G
BbvCI 4462 GCTGAGG CCTCAGC(-5/-2)
BcnI 1293 CCGGG CC^[GC]GG
BsaAI 2975 TACGTA [CT]AC^GT[GA]
BsaBI 4401 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2845 GTGCAG GTGCAG(16/14)
BsiEI 2602 CGACCG CG[GA][CT]^CG
BsiHKAI 5265 GAGCTC G[AT]GC[AT]^C
BsoBI 1952 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4620 TGTACA T^GTACA
BspLU11I 2841 ACATGT A^CATGT
BsrGI 4620 TGTACA T^GTACA
Bst1107I 561 GTATAC GTA^TAC
BstAPI 3796 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4217 CCACAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 561 GTATAC GTA^TAC
Bsu36I 2458 CCTAAGG CC^T[ACGT]AGG
DraIII 3458 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 428 GACAGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3441 AGCGCT AGC^GCT
Eco81I 2458 CCTAAGG CC^T[ACGT]AGG
EcoT22I 285 ATGCAT ATGCA^T
HaeII 3441 AGCGCT [GA]GCGC^[CT]
HapII 1293 CCGG C^CGG
HindIII 100 AAGCTT A^AGCTT
HpaI 3693 GTTAAC GTT^AAC
HpaII 1293 CCGG C^CGG
MamI 4401 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1293 CCGG C^CGG
NciI 1293 CCGGG CC^[GC]GG
NdeI 2365 CATATG CA^TATG
NheI 640 GCTAGC G^CTAGC
NsiI 285 ATGCAT ATGCA^T
PaeR7I 1952 CTCGAG C^TCGAG
PflFI 428 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1556 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 5265 GAGCTC GAGCT^C
SapI 5267 GCTCTTC GCTCTTC(1/4)
SnaBI 2975 TACGTA TAC^GTA
SphI 4087 GCATGC GCATG^C
SspBI 4620 TGTACA T^GTACA
Tth111I 428 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1556 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1575 TCTAGA T^CTAGA
XcmI 776 CCAACTTGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1952 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BalI 4214 TGGCCA TGG^CCA
5162 TGGCCA
BanII 1838 GGGCTC G[GA]GC[CT]^C
5265 GAGCTC
BmyI 1838 GGGCTC G[AGT]GC[ACT]^C
5265 GAGCTC
BpmI 175 CTCCAG CTGGAG(16/14)
3967 CTGGAG
BsiYI 1556 CCATTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4952 CCAAGGCTCGG
BslI 1556 CCATTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4952 CCAAGGCTCGG
BsmFI 490 GGGAC GGGAC(10/14)
1441 GTCCC
BsmI 5016 GAATGC GAATGC(1/-1)
509 GCATTC
Bsp1286I 1838 GGGCTC G[AGT]GC[ACT]^C
5265 GAGCTC
BspHI 114 TCATGA T^CATGA
2882 TCATGA
BssSI 1708 CACGAG CACGAG(-5/-1)
1103 CTCGTG
CfoI 900 GCGC GCG^C
3442 GCGC
EaeI 4214 TGGCCA [CT]^GGCC[GA]
5162 TGGCCA
EcoRI 379 GAATTC G^AATTC
3128 GAATTC
EcoRV 4608 GATATC GAT^ATC
4696 GATATC
HhaI 900 GCGC GCG^C
3442 GCGC
HinP1I 900 GCGC G^CGC
3442 GCGC
HincII 3693 GTTAAC GT[CT]^[GA]AC
4824 GTCAAC
HindII 3693 GTTAAC GT[CT]^[GA]AC
4824 GTCAAC
MfeI 62 CAATTG C^AATTG
2828 CAATTG
MluNI 4214 TGGCCA TGG^CCA
5162 TGGCCA
MscI 4214 TGGCCA TGG^CCA
5162 TGGCCA
MspA1I 3367 CAGCTG C[AC]G^C[GT]G
4519 CAGCTG
MunI 62 CAATTG C^AATTG
2828 CAATTG
PvuII 3367 CAGCTG CAG^CTG
4519 CAGCTG
RcaI 114 TCATGA T^CATGA
2882 TCATGA
StuI 70 AGGCCT AGG^CCT
3206 AGGCCT
SwaI 2379 ATTTAAAT ATTT^AAAT
3650 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AvaII
BbsI BbvI BclI BfaI BglII
BpuAI BsaJI BseRI BsmAI BsrDI
BsrI BssKI BstBI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NspI NspV PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI