Restriction map for
pF1KE2600
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EarI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI Hin1I HindIII HpaI
KasI KpnI Ksp632I MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 721 GTAC GT^AC
AhdI 583 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 502 GATCC GGATC(4/5)
AlwNI 313 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 583 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 90 CTCGGG C^[CT]CG[GA]G
AviII 217 TGCGCA TGC^GCA
BcgI 129 CGACGCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 531 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 618 CTCCTC GAGGAG(10/8)
BsmBI 477 CGTCTC CGTCTC(1/5)
BsoBI 90 CTCGGG C^[CT]CG[GA]G
Bsp1407I 720 TGTACA T^GTACA
BspMI 377 ACCTGC ACCTGC(4/8)
BsrGI 720 TGTACA T^GTACA
BstAPI 312 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 502 GATC ^GATC
DraII 102 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 490 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 327 CGGCCG [CT]^GGCC[GA]
EagI 327 CGGCCG C^GGCCG
Eam1105I 583 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 327 CGGCCG C^GGCCG
Eco52I 327 CGGCCG C^GGCCG
EcoO109I 102 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 848 CCAAGG C^C[AT][AT]GG
FspI 217 TGCGCA TGC^GCA
MaeIII 737 GTGAC ^GT[ACGT]AC
MamI 531 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 502 GATC ^GATC
NaeI 454 GCCGGC GCC^GGC
NdeII 502 GATC ^GATC
NgoMIV 454 GCCGGC G^CCGGC
PflMI 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 102 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 310 CTGCAG CTGCA^G
RsaI 721 GTAC GT^AC
SacI 87 GAGCTC GAGCT^C
Sau3AI 502 GATC ^GATC
SfaNI 245 GCATC GCATC(5/9)
SgrAI 65 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 720 TGTACA T^GTACA
StyI 848 CCAAGG C^C[AT][AT]GG
TfiI 681 GAATC G^A[AT]TC
Tsp45I 737 GTGAC ^GT[GC]AC
Van91I 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 137 GTGCAC G^TGCAC
865 GTGCAC
Aor51HI 257 AGCGCT AGC^GCT
569 AGCGCT
ApaLI 137 GTGCAC G^TGCAC
865 GTGCAC
AvaII 103 GGTCC G^G[AT]CC
649 GGTCC
BbsI 442 GAAGAC GAAGAC(2/6)
670 GAAGAC
BcnI 193 CCGGG CC^[GC]GG
492 CCGGG
BfaI 350 CTAG C^TAG
428 CTAG
BpmI 398 CTCCAG CTGGAG(16/14)
480 CTCCAG
BpuAI 442 GAAGAC GAAGAC(2/6)
670 GAAGAC
BsiEI 304 CGACCG CG[GA][CT]^CG
327 CGGCCG
BsrFI 66 GCCGGT [GA]^CCGG[CT]
454 GCCGGC
BsrI 141 ACTGG ACTGG(1/-1)
147 CCAGT
BssHII 152 GCGCGC G^CGCGC
369 GCGCGC
Cfr10I 66 GCCGGT [GA]^CCGG[CT]
454 GCCGGC
Eco47III 257 AGCGCT AGC^GCT
569 AGCGCT
EcoNI 775 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
829 CCTCTCCCAGG
HgaI 130 GACGC GACGC(5/10)
341 GCGTC
HincII 343 GTCAAC GT[CT]^[GA]AC
580 GTTGAC
HindII 343 GTCAAC GT[CT]^[GA]AC
580 GTTGAC
HphI 403 GGTGA GGTGA(8/7)
684 TCACC
KspI 57 CCGCGG CCGC^GG
330 CCGCGG
MaeI 350 CTAG C^TAG
428 CTAG
MaeII 214 ACGT A^CGT
863 ACGT
MboII 442 GAAGA GAAGA(8/7)
670 GAAGA
NciI 193 CCGGG CC^[GC]GG
492 CCGGG
NlaIII 553 CATG CATG^
879 CATG
NspI 552 GCATGC [GA]CATG^[CT]
878 GCATGC
SacII 57 CCGCGG CCGC^GG
330 CCGCGG
SfcI 310 CTGCAG C^T[GA][CT]AG
873 CTACAG
SphI 552 GCATGC GCATG^C
878 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NlaIV
PleI Sau96I ScrFI TseI TspRI