Restriction map for
pF1KE2678
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# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII Alw44I AocI ApaLI AscI
AseI AsnI AvrII BamHI BbeI
BbvCI BglII BlnI BseAI BsiWI
Bsp1407I BspEI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I DraI DrdI
EagI EclXI Eco52I Eco81I EcoRI
EcoRV FseI HpaI KasI KspI
MluI MroI NarI NheI NotI
NruI NspV PacI PaeR7I PmeI
PshBI SacII SfoI SfuI SgrAI
SnaBI SpeI SspBI StuI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 3816 GACGTC GACGT^C
AclI 2904 AACGTT AA^CGTT
AflII 1393 CTTAAG C^TTAAG
AgeI 3623 ACCGGT A^CCGGT
AhdI 916 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2868 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 916 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2705 TGCGCA TGC^GCA
BbrPI 762 CACGTG CAC^GTG
BcgI 3219 CGAGGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 792 GGATAC GTATCC(6/5)
BclI 1016 TGATCA T^GATCA
BfrI 1393 CTTAAG C^TTAAG
BlpI 4116 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4116 GCTCAGC GC^T[ACGT]AGC
BsaBI 1207 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1704 CGTCTC CGTCTC(1/5)
BsmI 2826 GCATTC GAATGC(1/-1)
BspDI 2998 ATCGAT AT^CGAT
BstAPI 3635 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4116 GCTCAGC GC^T[ACGT]AGC
ClaI 2998 ATCGAT AT^CGAT
CpoI 3625 CGGTCCG CG^G[AT]CCG
DraIII 3559 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 916 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1016 TGATCA T^GATCA
FspI 2705 TGCGCA TGC^GCA
HgaI 4240 GCGTC GACGC(5/10)
HincII 1567 GTCGAC GT[CT]^[GA]AC
HindII 1567 GTCGAC GT[CT]^[GA]AC
HindIII 458 AAGCTT A^AGCTT
MamI 1207 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 446 CAATTG C^AATTG
MunI 446 CAATTG C^AATTG
NdeI 842 CATATG CA^TATG
PinAI 3623 ACCGGT A^CCGGT
PmaCI 762 CACGTG CAC^GTG
PmlI 762 CACGTG CAC^GTG
PpuMI 602 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2777 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2904 AACGTT AA^CGTT
RsrII 3625 CGGTCCG CG^G[AT]CCG
SacI 74 GAGCTC GAGCT^C
SalI 1567 GTCGAC G^TCGAC
SbfI 3777 CCTGCAGG CCTGCA^GG
SfiI 48 GGCCGTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 197 CCCGGG CCC^GGG
SphI 2106 GCATGC GCATG^C
Sse8387I 3777 CCTGCAGG CCTGCA^GG
SspI 2080 AATATT AAT^ATT
XmaCI 197 CCCGGG C^CCGGG
XmaI 197 CCCGGG C^CCGGG
XmnI 2868 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 192 GGTACC G^GTACC
3576 GGTACC
AccI 1567 GTCGAC GT^[AC][GT]AC
3445 GTAGAC
AccII 3490 CGCG CG^CG
3956 CGCG
AcyI 3816 GACGTC G[GA]^CG[CT]C
4239 GGCGTC
Aor51HI 1663 AGCGCT AGC^GCT
3359 AGCGCT
ApaI 3511 GGGCCC GGGCC^C
4265 GGGCCC
Asp718I 192 GGTACC G^GTACC
3576 GGTACC
AspI 3493 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4138 GACCGGGTC
AvaI 197 CCCGGG C^[CT]CG[GA]G
3699 CTCGGG
BbsI 2461 GTCTTC GAAGAC(2/6)
2581 GTCTTC
BfaI 595 CTAG C^TAG
4433 CTAG
BpmI 4401 CTCCAG CTGGAG(16/14)
1231 CTGGAG
BpuAI 2461 GTCTTC GAAGAC(2/6)
2581 GTCTTC
BsaAI 762 CACGTG [CT]AC^GT[GA]
3442 CACGTA
BsaHI 3816 GACGTC G[GA]^CG[CT]C
4239 GGCGTC
BsoBI 197 CCCGGG C^[CT]CG[GA]G
3699 CTCGGG
BstEII 1440 GGTTACC G^GT[ACGT]ACC
2244 GGTGACC
BstPI 1440 GGTTACC G^GT[ACGT]ACC
2244 GGTGACC
BstUI 3490 CGCG CG^CG
3956 CGCG
Eco47III 1663 AGCGCT AGC^GCT
3359 AGCGCT
EcoO65I 1440 GGTTACC G^GT[ACGT]ACC
2244 GGTGACC
EcoT22I 2104 ATGCAT ATGCA^T
2147 ATGCAT
Hin1I 3816 GACGTC G[GA]^CG[CT]C
4239 GGCGTC
KpnI 192 GGTACC GGTAC^C
3576 GGTACC
MaeI 595 CTAG C^TAG
4433 CTAG
MvnI 3490 CGCG CG^CG
3956 CGCG
NaeI 3795 GCCGGC GCC^GGC
3896 GCCGGC
NgoMIV 3795 GCCGGC G^CCGGC
3896 GCCGGC
NsiI 2104 ATGCAT ATGCA^T
2147 ATGCAT
PflFI 3493 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4138 GACCGGGTC
PvuI 1555 CGATCG CGAT^CG
2627 CGATCG
SapI 87 GAAGAGC GCTCTTC(1/4)
1195 GAAGAGC
ScaI 1160 AGTACT AGT^ACT
1733 AGTACT
SexAI 1436 ACCTGGT A^CC[AT]GGT
1982 ACCAGGT
SmlI 658 CTCAAG C^T[CT][GA]AG
1393 CTTAAG
Tth111I 3493 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4138 GACCGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII