Restriction map for
pF1KE2114
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AflII Alw44I Aor51HI ApaLI
AscI AspI AviII AvrII BcgI
BciVI BfrI BglII BlnI BsiWI
BsmBI Bsp1407I BspDI BsrGI BssHII
BstAPI BstBI ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FseI FspI
HindIII HpaI KspI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PpuMI
PshAI PvuI RsrII SacII SbfI
ScaI SfiI SfuI SgrAI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 71 enzymes) #
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Acc65I 1377 GGTACC G^GTACC
AccIII 1608 TCCGGA T^CCGGA
AclI 2251 AACGTT AA^CGTT
AgeI 181 ACCGGT A^CCGGT
AhdI 1262 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1091 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1825 CCTCAGG CC^T[ACGT]AGG
ApaI 3 GGGCCC GGGCC^C
AseI 1603 ATTAAT AT^TAAT
AsnI 1603 ATTAAT AT^TAAT
Asp718I 1377 GGTACC G^GTACC
AspEI 1262 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 579 TGGCCA TGG^CCA
BamHI 729 GGATCC G^GATCC
BbeI 128 GGCGCC GGCGC^C
BbrPI 1663 CACGTG CAC^GTG
BbsI 449 GAAGAC GAAGAC(2/6)
BbvCI 912 CCTCAGC CCTCAGC(-5/-2)
BfaI 2528 CTAG C^TAG
BglI 83 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1636 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1636 GCTGAGC GC^T[ACGT]AGC
BpuAI 449 GAAGAC GAAGAC(2/6)
BseAI 1608 TCCGGA T^CCGGA
BsgI 2314 CTGCAC GTGCAG(16/14)
BsiEI 183 CGGTCG CG[GA][CT]^CG
BsmI 717 GAATGC GAATGC(1/-1)
BspEI 1608 TCCGGA T^CCGGA
BspHI 2372 TCATGA T^CATGA
BspLU11I 2621 ACATGT A^CATGT
BspMI 45 GCAGGT ACCTGC(4/8)
BsrBI 2201 GAGCGG CCGCTC(-3/-3)
BsrDI 672 CATTGC GCAATG(2/0)
BsrFI 181 ACCGGT [GA]^CCGG[CT]
BssSI 2491 CTCGTG CACGAG(-5/-1)
Bsu36I 1825 CCTCAGG CC^T[ACGT]AGG
CelII 1636 GCTGAGC GC^T[ACGT]AGC
Cfr10I 181 ACCGGT [GA]^CCGG[CT]
DraIII 697 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1421 CCGTGG C^C[GA][CT]GG
EaeI 579 TGGCCA [CT]^GGCC[GA]
Eam1105I 1262 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1825 CCTCAGG CC^T[ACGT]AGG
HincII 185 GTCGAC GT[CT]^[GA]AC
HindII 185 GTCGAC GT[CT]^[GA]AC
KasI 128 GGCGCC G^GCGCC
KpnI 1377 GGTACC GGTAC^C
MaeI 2528 CTAG C^TAG
MluNI 579 TGGCCA TGG^CCA
MroI 1608 TCCGGA T^CCGGA
MscI 579 TGGCCA TGG^CCA
NarI 128 GGCGCC GG^CGCC
NspI 2621 ACATGT [GA]CATG^[CT]
PflMI 288 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 181 ACCGGT A^CCGGT
PmaCI 1663 CACGTG CAC^GTG
PmlI 1663 CACGTG CAC^GTG
PshBI 1603 ATTAAT AT^TAAT
Psp1406I 2251 AACGTT AA^CGTT
PstI 43 CTGCAG CTGCA^G
RcaI 2372 TCATGA T^CATGA
SalI 185 GTCGAC G^TCGAC
SapI 823 GCTCTTC GCTCTTC(1/4)
SexAI 521 ACCTGGT A^CC[AT]GGT
SfoI 128 GGCGCC GGC^GCC
SmaI 2243 CCCGGG CCC^GGG
SnaBI 1017 TACGTA TAC^GTA
Van91I 288 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1412 CCACCGTCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2243 CCCGGG C^CCGGG
XmaI 2243 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 148 CGCG CG^CG
864 CGCG
Asp700I 323 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2510 GAAGCGGTTC
AvaI 1631 CTCGGG C^[CT]CG[GA]G
2243 CCCGGG
BclI 746 TGATCA T^GATCA
1085 TGATCA
BsaBI 741 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2434 GATGAAAATC
BsaI 48 GGTCTC GGTCTC(1/5)
1389 GGTCTC
BsaWI 181 ACCGGT [AT]^CCGG[AT]
1608 TCCGGA
BsoBI 1631 CTCGGG C^[CT]CG[GA]G
2243 CCCGGG
Bst1107I 1020 GTATAC GTA^TAC
1234 GTATAC
BstEII 753 GGTAACC G^GT[ACGT]ACC
984 GGTCACC
BstPI 753 GGTAACC G^GT[ACGT]ACC
984 GGTCACC
BstUI 148 CGCG CG^CG
864 CGCG
BstXI 989 CCACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1589 CCAACTGGCTGG
BstZ17I 1020 GTATAC GTA^TAC
1234 GTATAC
CfoI 28 GCGC GCG^C
129 GCGC
DraII 3 GGGCCCT [GA]G^G[ACGT]CC[CT]
2321 GGGGCCT
EcoO109I 3 GGGCCCT [GA]G^G[ACGT]CC[CT]
2321 GGGGCCT
EcoO65I 753 GGTAACC G^GT[ACGT]ACC
984 GGTCACC
EcoRI 781 GAATTC G^AATTC
2147 GAATTC
EcoT14I 7 CCTTGG C^C[AT][AT]GG
582 CCAAGG
FbaI 746 TGATCA T^GATCA
1085 TGATCA
HaeII 27 GGCGCT [GA]GCGC^[CT]
128 GGCGCC
HhaI 28 GCGC GCG^C
129 GCGC
HinP1I 28 GCGC G^CGC
129 GCGC
MamI 741 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2434 GATGAAAATC
MvnI 148 CGCG CG^CG
864 CGCG
SacI 928 GAGCTC GAGCT^C
2535 GAGCTC
SmlI 1999 CTCAAG C^T[CT][GA]AG
2304 CTTGAG
StuI 259 AGGCCT AGG^CCT
386 AGGCCT
StyI 7 CCTTGG C^C[AT][AT]GG
582 CCAAGG
XmnI 323 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2510 GAAGCGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII