Restriction map for
pF1KE4224
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmI BspEI BspLU11I
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CpoI DraI DraII DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I FauI FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1837 GTCTAC GT^[AC][GT]AC
AccII 67 CGCG CG^CG
AcyI 1725 GACGCC G[GA]^CG[CT]C
AhdI 147 GACGAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 147 GACGAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 62 GGATCC G^GATCC
BciVI 2208 GTATCC GTATCC(6/5)
BfaI 2299 CTAG C^TAG
BglI 1785 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 922 AGATCT A^GATCT
BsaAI 260 TACGTG [CT]AC^GT[GA]
BsaHI 1725 GACGCC G[GA]^CG[CT]C
BsiEI 2041 CGATCG CG[GA][CT]^CG
BsmBI 1931 CGTCTC CGTCTC(1/5)
BspDI 710 ATCGAT AT^CGAT
BspMI 1756 ACCTGC ACCTGC(4/8)
BsrDI 1316 GCAATG GCAATG(2/0)
BsrFI 509 ACCGGC [GA]^CCGG[CT]
BstUI 67 CGCG CG^CG
BstXI 1904 CCAGCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 509 ACCGGC [GA]^CCGG[CT]
ClaI 710 ATCGAT AT^CGAT
DrdI 2037 GACCCGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1832 TGGCCG [CT]^GGCC[GA]
Eam1105I 147 GACGAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1335 GAATTC G^AATTC
EcoRV 490 GATATC GAT^ATC
HgaI 1725 GACGC GACGC(5/10)
Hin1I 1725 GACGCC G[GA]^CG[CT]C
MaeI 2299 CTAG C^TAG
MslI 517 CACTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 67 CGCG CG^CG
PvuI 2041 CGATCG CGAT^CG
SapI 739 GAAGAGC GCTCTTC(1/4)
SmaI 2119 CCCGGG CCC^GGG
SspI 1678 AATATT AAT^ATT
StuI 832 AGGCCT AGG^CCT
XcmI 1820 CCAAGAGGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2119 CCCGGG C^CCGGG
XmaI 2119 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BbsI 1356 GAAGAC GAAGAC(2/6)
1394 GTCTTC
BpuAI 1356 GAAGAC GAAGAC(2/6)
1394 GTCTTC
BseRI 2144 CTCCTC GAGGAG(10/8)
1389 GAGGAG
Bsp1407I 1247 TGTACA T^GTACA
2271 TGTACA
BspHI 746 TCATGA T^CATGA
1409 TCATGA
BsrGI 1247 TGTACA T^GTACA
2271 TGTACA
BssSI 1223 CACGAG CACGAG(-5/-1)
1469 CTCGTG
BstYI 62 GGATCC [GA]^GATC[CT]
922 AGATCT
CfoI 68 GCGC GCG^C
1783 GCGC
DsaI 46 CCGTGG C^C[GA][CT]GG
1829 CCGTGG
EcoT22I 243 ATGCAT ATGCA^T
1203 ATGCAT
HhaI 68 GCGC GCG^C
1783 GCGC
HinP1I 68 GCGC G^CGC
1783 GCGC
MaeII 261 ACGT A^CGT
2345 ACGT
MflI 62 GGATCC [GA]^GATC[CT]
922 AGATCT
NsiI 243 ATGCAT ATGCA^T
1203 ATGCAT
NspI 872 GCATGT [GA]CATG^[CT]
1312 ACATGC
PflMI 1485 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1980 CCACCTTCTGG
PvuII 1946 CAGCTG CAG^CTG
2099 CAGCTG
RcaI 746 TCATGA T^CATGA
1409 TCATGA
SacI 112 GAGCTC GAGCT^C
742 GAGCTC
SspBI 1247 TGTACA T^GTACA
2271 TGTACA
Van91I 1485 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1980 CCACCTTCTGG
XhoII 62 GGATCC [GA]^GATC[CT]
922 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PaeR7I PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoI