Restriction map for
pF1KE9257
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# NO CUT ENZYMES ( 87 enzymes) #
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AccI AccIII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI AspEI AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI HindIII KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI PacI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SacII SalI SbfI
ScaI SnaBI SpeI Sse8387I SspBI
SwaI XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1789 GACGTC GACGT^C
Acc65I 2441 GGTACC G^GTACC
AcsI 1186 GAATTT [GA]^AATT[CT]
AflIII 1529 ACACGT A^C[GA][CT]GT
ApoI 1186 GAATTT [GA]^AATT[CT]
Asp700I 1401 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2441 GGTACC G^GTACC
AviII 2558 TGCGCA TGC^GCA
BbeI 1113 GGCGCC GGCGC^C
BclI 1091 TGATCA T^GATCA
BsaI 2510 GAGACC GGTCTC(1/5)
BsiEI 1119 CGATCG CG[GA][CT]^CG
BspHI 494 TCATGA T^CATGA
BspMI 2031 GCAGGT ACCTGC(4/8)
BssSI 2044 CACGAG CACGAG(-5/-1)
BstBI 133 TTCGAA TT^CGAA
BstXI 2484 CCATACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 1420 CTCAG C^T[ACGT]AG
EcoRV 178 GATATC GAT^ATC
FbaI 1091 TGATCA T^GATCA
FspI 2558 TGCGCA TGC^GCA
HincII 208 GTTAAC GT[CT]^[GA]AC
HindII 208 GTTAAC GT[CT]^[GA]AC
HpaI 208 GTTAAC GTT^AAC
KasI 1113 GGCGCC G^GCGCC
KpnI 2441 GGTACC GGTAC^C
NaeI 13 GCCGGC GCC^GGC
NarI 1113 GGCGCC GG^CGCC
NcoI 1931 CCATGG C^CATGG
NgoMIV 13 GCCGGC G^CCGGC
NspV 133 TTCGAA TT^CGAA
PaeR7I 1431 CTCGAG C^TCGAG
PpuMI 94 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1284 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1119 CGATCG CGAT^CG
RcaI 494 TCATGA T^CATGA
SacI 1942 GAGCTC GAGCT^C
SapI 2142 GCTCTTC GCTCTTC(1/4)
SfiI 2427 GGCCTTCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1113 GGCGCC GGC^GCC
SfuI 133 TTCGAA TT^CGAA
SgrAI 1751 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2098 CCCGGG CCC^GGG
SmlI 1431 CTCGAG C^T[CT][GA]AG
SphI 1445 GCATGC GCATG^C
SspI 646 AATATT AAT^ATT
XhoI 1431 CTCGAG C^TCGAG
XmaCI 2098 CCCGGG C^CCGGG
XmaI 2098 CCCGGG C^CCGGG
XmnI 1401 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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Alw44I 915 GTGCAC G^TGCAC
1383 GTGCAC
AlwNI 478 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1672 CAGACCCTG
ApaLI 915 GTGCAC G^TGCAC
1383 GTGCAC
BanII 1504 GAGCCC G[GA]GC[CT]^C
1942 GAGCTC
BglI 1116 GCCCGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2428 GCCTTCCGGGC
BglII 254 AGATCT A^GATCT
1427 AGATCT
BpmI 343 CTGGAG CTGGAG(16/14)
1021 CTGGAG
BsaAI 1530 CACGTA [CT]AC^GT[GA]
2368 TACGTG
BsmBI 469 GAGACG CGTCTC(1/5)
1436 GAGACG
BsrBI 897 GAGCGG CCGCTC(-3/-3)
2164 GAGCGG
BstAPI 28 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1632 GCAGTGCGTGC
DraI 244 TTTAAA TTT^AAA
718 TTTAAA
DraII 94 GGGACCT [GA]G^G[ACGT]CC[CT]
479 AGGCCCT
EcoO109I 94 GGGACCT [GA]G^G[ACGT]CC[CT]
479 AGGCCCT
NspI 1307 ACATGC [GA]CATG^[CT]
1445 GCATGC
PvuII 1546 CAGCTG CAG^CTG
2353 CAGCTG
SexAI 2420 ACCTGGT A^CC[AT]GGT
2447 ACCTGGT
StuI 2066 AGGCCT AGG^CCT
2281 AGGCCT
XcmI 1829 CCATTGCCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2438 CCAGGTACCACCTGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AspI AvaI AvaII
BalI BanI BbvI BcnI BfaI
BmyI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraIII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII