Restriction map for
pF1KB6977
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# NO CUT ENZYMES ( 159 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 18 GACGTC GACGT^C
AccII 124 CGCG CG^CG
AfaI 384 GTAC GT^AC
AocI 354 CCTCAGG CC^T[ACGT]AGG
AspI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 423 GGACC G^G[AT]CC
AviII 608 TGCGCA TGC^GCA
BanI 388 GGCGCC G^G[CT][GA]CC
BanII 340 GAGCCC G[GA]GC[CT]^C
BbeI 388 GGCGCC GGCGC^C
BbsI 364 GTCTTC GAAGAC(2/6)
BglII 488 AGATCT A^GATCT
BmyI 340 GAGCCC G[AGT]GC[ACT]^C
BpmI 337 CTGGAG CTGGAG(16/14)
BpuAI 364 GTCTTC GAAGAC(2/6)
BsmAI 598 GAGAC GTCTC(1/5)
BsmFI 21 GTCCC GGGAC(10/14)
Bsp1286I 340 GAGCCC G[AGT]GC[ACT]^C
BsrBI 624 CCGCTC CCGCTC(-3/-3)
BstUI 124 CGCG CG^CG
BstXI 371 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 488 AGATCT [GA]^GATC[CT]
Bsu36I 354 CCTCAGG CC^T[ACGT]AGG
Cfr13I 423 GGACC G^G[ACGT]CC
DpnII 489 GATC ^GATC
DraII 422 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 397 GACAGCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 354 CCTCAGG CC^T[ACGT]AGG
EcoO109I 422 AGGACCT [GA]G^G[ACGT]CC[CT]
FspI 608 TGCGCA TGC^GCA
HaeII 388 GGCGCC [GA]GCGC^[CT]
HgaI 538 GACGC GACGC(5/10)
HinfI 244 GAGTC G^A[ACGT]TC
KasI 388 GGCGCC G^GCGCC
MboI 489 GATC ^GATC
MflI 488 AGATCT [GA]^GATC[CT]
MslI 529 CACAACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 124 CGCG CG^CG
NarI 388 GGCGCC GG^CGCC
NdeII 489 GATC ^GATC
NlaIII 585 CATG CATG^
PflFI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 244 GAGTC GAGTC(4/5)
PpuMI 422 AGGACCT [GA]G^G[AT]CC[CT]
PstI 211 CTGCAG CTGCA^G
RsaI 384 GTAC GT^AC
Sau3AI 489 GATC ^GATC
Sau96I 423 GGACC G^G[ACGT]CC
SfoI 388 GGCGCC GGC^GCC
SmlI 547 CTCAAG C^T[CT][GA]AG
Tth111I 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 488 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 18 GACGTC G[GA]^CG[CT]C
388 GGCGCC
BcnI 14 CCCGG CC^[GC]GG
476 CCCGG
BfaI 295 CTAG C^TAG
427 CTAG
BsaHI 18 GACGTC G[GA]^CG[CT]C
388 GGCGCC
BsaJI 55 CCCTGG C^C[ACGT][ACGT]GG
475 CCCCGG
BsrI 481 ACTGG ACTGG(1/-1)
327 CCAGT
BstF5I 324 CATCC GGATG(2/0)
438 CATCC
DdeI 240 CTCAG C^T[ACGT]AG
355 CTCAG
FokI 324 CATCC GGATG(9/13)
438 CATCC
HaeIII 40 GGCC GG^CC
147 GGCC
HapII 15 CCGG C^CGG
477 CCGG
Hin1I 18 GACGTC G[GA]^CG[CT]C
388 GGCGCC
HpaII 15 CCGG C^CGG
477 CCGG
MaeI 295 CTAG C^TAG
427 CTAG
MaeII 19 ACGT A^CGT
404 ACGT
MspA1I 142 CAGCTG C[AC]G^C[GT]G
639 CAGCTG
MspI 15 CCGG C^CGG
477 CCGG
NciI 14 CCCGG CC^[GC]GG
476 CCCGG
PvuII 142 CAGCTG CAG^CTG
639 CAGCTG
TaqI 515 TCGA T^CGA
596 TCGA
TspRI 72 CAGTG CA[GC]TG[ACGT][ACGT]^
619 CAGTG
TthHB8I 515 TCGA T^CGA
596 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BbvI BsgI BsiYI
BslI BssKI BstNI Cac8I CfoI
EcoNI EcoRII Fnu4HI HhaI HinP1I
ItaI MboII MnlI MvaI MwoI
NlaIV ScrFI SfaNI SfcI TseI