Restriction map for
pF1KE2499
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# NO CUT ENZYMES ( 41 enzymes) #
##################################
AclI AflII AscI AseI AsnI
AvrII BciVI BfaI BfrI BlnI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
CpoI DraI EcoT22I HpaI MaeI
MfeI MluI MunI NdeI NheI
NruI NsiI NspV PacI PmeI
PshBI Psp1406I PvuI RsrII SalI
SfuI SnaBI SpeI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 602 GGTACC G^GTACC
AccIII 7298 TCCGGA T^CCGGA
AflIII 4878 ACACGT A^C[GA][CT]GT
Asp718I 602 GGTACC G^GTACC
AspI 13081 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 12832 GGATCC G^GATCC
BseAI 7298 TCCGGA T^CCGGA
BsiWI 4039 CGTACG C^GTACG
BsmI 4331 GCATTC GAATGC(1/-1)
Bsp1407I 10751 TGTACA T^GTACA
BspDI 12694 ATCGAT AT^CGAT
BspEI 7298 TCCGGA T^CCGGA
BspHI 9354 TCATGA T^CATGA
BsrGI 10751 TGTACA T^GTACA
ClaI 12694 ATCGAT AT^CGAT
EcoRI 1780 GAATTC G^AATTC
EcoRV 523 GATATC GAT^ATC
HindIII 7560 AAGCTT A^AGCTT
KpnI 602 GGTACC GGTAC^C
MroI 7298 TCCGGA T^CCGGA
MseI 1052 TTAA T^TAA
NotI 5890 GCGGCCGC GC^GGCCGC
PflFI 13081 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 10086 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 9354 TCATGA T^CATGA
ScaI 9860 AGTACT AGT^ACT
SexAI 12983 ACCTGGT A^CC[AT]GGT
SphI 4268 GCATGC GCATG^C
SspBI 10751 TGTACA T^GTACA
TfiI 9939 GATTC G^A[AT]TC
Tru9I 1052 TTAA T^TAA
Tth111I 13081 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AatII 8788 GACGTC GACGT^C
12468 GACGTC
AccI 8764 GTCTAC GT^[AC][GT]AC
11164 GTCTAC
AcsI 1780 GAATTC [GA]^AATT[CT]
6595 AAATTC
AgeI 11998 ACCGGT A^CCGGT
13057 ACCGGT
AhdI 820 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
11584 GACCAGCTGTC
ApoI 1780 GAATTC [GA]^AATT[CT]
6595 AAATTC
Asp700I 216 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
13140 GAAGGCCTTC
AspEI 820 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
11584 GACCAGCTGTC
BclI 10934 TGATCA T^GATCA
12659 TGATCA
Eam1105I 820 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
11584 GACCAGCTGTC
FbaI 10934 TGATCA T^GATCA
12659 TGATCA
NcoI 11759 CCATGG C^CATGG
12192 CCATGG
NspI 4268 GCATGC [GA]CATG^[CT]
12782 ACATGC
PinAI 11998 ACCGGT A^CCGGT
13057 ACCGGT
Tsp509I 1781 AATT ^AATT
6596 AATT
XmnI 216 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
13140 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 169 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PaeR7I PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfiI SfoI SgrAI SmaI SmlI
Sse8387I StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI