Restriction map for
pF1KE4189
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AvrII BamHI BbeI BbrPI BbvCI
BciVI BfrI BglI BlnI BsaBI
BsaHI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoT22I FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MunI NarI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 521 GGTACC G^GTACC
AccI 358 GTCTAC GT^[AC][GT]AC
AccII 1827 CGCG CG^CG
AccIII 45 TCCGGA T^CCGGA
AflIII 398 ACACGT A^C[GA][CT]GT
Alw44I 1263 GTGCAC G^TGCAC
ApaLI 1263 GTGCAC G^TGCAC
Asp718I 521 GGTACC G^GTACC
AviII 644 TGCGCA TGC^GCA
BclI 1079 TGATCA T^GATCA
BglII 198 AGATCT A^GATCT
BsaAI 399 CACGTA [CT]AC^GT[GA]
BseAI 45 TCCGGA T^CCGGA
BsiEI 1635 CGGCCG CG[GA][CT]^CG
BspEI 45 TCCGGA T^CCGGA
BspMI 1508 GCAGGT ACCTGC(4/8)
BsrFI 1677 GCCGGC [GA]^CCGG[CT]
BstAPI 1299 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1827 CGCG CG^CG
BstXI 1996 CCAGTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 645 GCGC GCG^C
Cfr10I 1677 GCCGGC [GA]^CCGG[CT]
DrdI 118 GACACTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1635 CGGCCG C^GGCCG
EclXI 1635 CGGCCG C^GGCCG
Eco52I 1635 CGGCCG C^GGCCG
EcoRI 325 GAATTC G^AATTC
EcoRV 229 GATATC GAT^ATC
FbaI 1079 TGATCA T^GATCA
FspI 644 TGCGCA TGC^GCA
HhaI 645 GCGC GCG^C
HinP1I 645 GCGC G^CGC
KpnI 521 GGTACC GGTAC^C
MroI 45 TCCGGA T^CCGGA
MvnI 1827 CGCG CG^CG
NaeI 1677 GCCGGC GCC^GGC
NgoMIV 1677 GCCGGC G^CCGGC
NotI 1634 GCGGCCGC GC^GGCCGC
NspI 140 ACATGC [GA]CATG^[CT]
PflMI 1590 CCAAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1286 GGGACCT [GA]G^G[AT]CC[CT]
SbfI 1866 CCTGCAGG CCTGCA^GG
SmlI 985 CTTGAG C^T[CT][GA]AG
Sse8387I 1866 CCTGCAGG CCTGCA^GG
TaqI 116 TCGA T^CGA
TfiI 251 GATTC G^A[AT]TC
TthHB8I 116 TCGA T^CGA
Van91I 1590 CCAAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 937 CCCGAG C^[CT]CG[GA]G
1583 CTCGGG
BanI 456 GGTGCC G^G[CT][GA]CC
521 GGTACC
BbsI 735 GAAGAC GAAGAC(2/6)
793 GAAGAC
BcgI 939 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1189 GCACTCAACTCG
BlpI 747 GCTGAGC GC^T[ACGT]AGC
1210 GCTGAGC
Bpu1102I 747 GCTGAGC GC^T[ACGT]AGC
1210 GCTGAGC
BpuAI 735 GAAGAC GAAGAC(2/6)
793 GAAGAC
BsoBI 937 CCCGAG C^[CT]CG[GA]G
1583 CTCGGG
BsrBI 697 GAGCGG CCGCTC(-3/-3)
1114 GAGCGG
BstYI 198 AGATCT [GA]^GATC[CT]
469 GGATCT
CelII 747 GCTGAGC GC^T[ACGT]AGC
1210 GCTGAGC
EcoT14I 281 CCAAGG C^C[AT][AT]GG
671 CCAAGG
HgaI 1384 GACGC GACGC(5/10)
1089 GCGTC
MflI 198 AGATCT [GA]^GATC[CT]
469 GGATCT
MseI 1004 TTAA T^TAA
1226 TTAA
MslI 234 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1266 CACATTCGTG
StyI 281 CCAAGG C^C[AT][AT]GG
671 CCAAGG
Tru9I 1004 TTAA T^TAA
1226 TTAA
XhoII 198 AGATCT [GA]^GATC[CT]
469 GGATCT
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI StuI TseI Tsp45I
Tsp509I TspRI