Restriction map for
pF1KE2607
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI AspEI AvrII BbrPI BclI
BfrI BglI BlnI BsiEI BsiWI
BsmI Bsp1407I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HpaI KspI MfeI
MluI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SfiI SfuI
SgrAI SpeI Sse8387I SspBI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 2761 GGTACC G^GTACC
AccI 2149 GTCGAC GT^[AC][GT]AC
Aor51HI 2681 AGCGCT AGC^GCT
Asp700I 3078 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2761 GGTACC G^GTACC
AspI 2971 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 482 TGCGCA TGC^GCA
BamHI 2660 GGATCC G^GATCC
BanII 2716 GAGCCC G[GA]GC[CT]^C
BbeI 451 GGCGCC GGCGC^C
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
BcgI 1818 CGACATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3255 GGATAC GTATCC(6/5)
BglII 488 AGATCT A^GATCT
BlpI 21 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 21 GCTCAGC GC^T[ACGT]AGC
BsmBI 83 CGTCTC CGTCTC(1/5)
BspHI 3173 TCATGA T^CATGA
BspLU11I 2888 ACATGT A^CATGT
BsrFI 497 GCCGGC [GA]^CCGG[CT]
BssSI 784 CTCGTG CACGAG(-5/-1)
CelII 21 GCTCAGC GC^T[ACGT]AGC
Cfr10I 497 GCCGGC [GA]^CCGG[CT]
DraII 2559 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 188 CCACGG C^C[GA][CT]GG
Eco47III 2681 AGCGCT AGC^GCT
EcoO109I 2559 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1539 GAATTC G^AATTC
FauI 1088 CCCGC CCCGC(4/6)
FspI 482 TGCGCA TGC^GCA
KasI 451 GGCGCC G^GCGCC
KpnI 2761 GGTACC GGTAC^C
NaeI 497 GCCGGC GCC^GGC
NarI 451 GGCGCC GG^CGCC
NgoMIV 497 GCCGGC G^CCGGC
NheI 1018 GCTAGC G^CTAGC
PflFI 2971 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2559 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 3173 TCATGA T^CATGA
SalI 2149 GTCGAC G^TCGAC
ScaI 3011 AGTACT AGT^ACT
SfoI 451 GGCGCC GGC^GCC
SmaI 1868 CCCGGG CCC^GGG
SmlI 3274 CTCAAG C^T[CT][GA]AG
SnaBI 2359 TACGTA TAC^GTA
SphI 479 GCATGC GCATG^C
SspI 622 AATATT AAT^ATT
Tth111I 2971 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2900 CCAACATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1868 CCCGGG C^CCGGG
XmaI 1868 CCCGGG C^CCGGG
XmnI 3078 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 830 TCCGGA T^CCGGA
1604 TCCGGA
AcyI 301 GACGCC G[GA]^CG[CT]C
451 GGCGCC
AflIII 1655 ACGTGT A^C[GA][CT]GT
2888 ACATGT
AlwNI 1752 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1924 CAGCTGCTG
AocI 1108 CCTGAGG CC^T[ACGT]AGG
2664 CCTTAGG
AspHI 90 GAGCAC G[AT]GC[AT]^C
2833 GAGCAC
BfaI 841 CTAG C^TAG
1019 CTAG
BsaBI 2586 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2862 GATCACCATC
BsaHI 301 GACGCC G[GA]^CG[CT]C
451 GGCGCC
BsaI 1864 GAGACC GGTCTC(1/5)
657 GGTCTC
BseAI 830 TCCGGA T^CCGGA
1604 TCCGGA
BsiHKAI 90 GAGCAC G[AT]GC[AT]^C
2833 GAGCAC
BspDI 2593 ATCGAT AT^CGAT
3239 ATCGAT
BspEI 830 TCCGGA T^CCGGA
1604 TCCGGA
BspMI 2840 ACCTGC ACCTGC(4/8)
2918 ACCTGC
BsrDI 1214 GCAATG GCAATG(2/0)
2040 GCAATG
Bsu36I 1108 CCTGAGG CC^T[ACGT]AGG
2664 CCTTAGG
ClaI 2593 ATCGAT AT^CGAT
3239 ATCGAT
Eco81I 1108 CCTGAGG CC^T[ACGT]AGG
2664 CCTTAGG
EcoNI 1023 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3000 CCTGGTGCAGG
HaeII 451 GGCGCC [GA]GCGC^[CT]
2681 AGCGCT
Hin1I 301 GACGCC G[GA]^CG[CT]C
451 GGCGCC
HincII 2149 GTCGAC GT[CT]^[GA]AC
2914 GTCAAC
HindII 2149 GTCGAC GT[CT]^[GA]AC
2914 GTCAAC
MaeI 841 CTAG C^TAG
1019 CTAG
MamI 2586 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2862 GATCACCATC
MroI 830 TCCGGA T^CCGGA
1604 TCCGGA
NspI 479 GCATGC [GA]CATG^[CT]
2888 ACATGT
PflMI 225 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2069 CCAAGAACTGG
SexAI 428 ACCTGGT A^CC[AT]GGT
2972 ACCAGGT
Tsp45I 442 GTGAC ^GT[GC]AC
2750 GTGAC
Van91I 225 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2069 CCAAGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BalI
BanI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaAI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraIII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII