Restriction map for
pF1KE2329
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Aor51HI AseI AsnI
Asp718I AspI AvrII BbrPI BcgI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BsrDI BssSI BstAPI BstBI
BstEII BstPI ClaI CpoI DrdI
Eco47III EcoNI EcoO65I EcoRV FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SpeI StuI
SwaI Tth111I Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AhdI 2630 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3851 GTGCAC G^TGCAC
ApaLI 3851 GTGCAC G^TGCAC
AscI 420 GGCGCGCC GG^CGCGCC
Asp700I 3645 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2630 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3399 TGCGCA TGC^GCA
BamHI 1188 GGATCC G^GATCC
BbsI 1845 GTCTTC GAAGAC(2/6)
BclI 2492 TGATCA T^GATCA
BpuAI 1845 GTCTTC GAAGAC(2/6)
Bsp1407I 3750 TGTACA T^GTACA
BspHI 3291 TCATGA T^CATGA
BsrGI 3750 TGTACA T^GTACA
BssHII 421 GCGCGC G^CGCGC
BstXI 3477 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1961 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2630 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3314 ATGCAT ATGCA^T
FbaI 2492 TGATCA T^GATCA
FspI 3399 TGCGCA TGC^GCA
NotI 10 GCGGCCGC GC^GGCCGC
NsiI 3314 ATGCAT ATGCA^T
PaeR7I 1367 CTCGAG C^TCGAG
PstI 2276 CTGCAG CTGCA^G
RcaI 3291 TCATGA T^CATGA
SacI 201 GAGCTC GAGCT^C
SbfI 2275 CCTGCAGG CCTGCA^GG
SnaBI 1239 TACGTA TAC^GTA
SphI 3303 GCATGC GCATG^C
Sse8387I 2275 CCTGCAGG CCTGCA^GG
SspBI 3750 TGTACA T^GTACA
SspI 2293 AATATT AAT^ATT
Tsp45I 2638 GTCAC ^GT[GC]AC
XhoI 1367 CTCGAG C^TCGAG
XmnI 3645 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AlwNI 1814 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2547 CAGCAACTG
AocI 2632 CCTGAGG CC^T[ACGT]AGG
3739 CCTCAGG
ApaI 469 GGGCCC GGGCC^C
3069 GGGCCC
BbeI 343 GGCGCC GGCGC^C
382 GGCGCC
BbvCI 3127 CCTCAGC CCTCAGC(-5/-2)
3439 CCTCAGC
BsaAI 1239 TACGTA [CT]AC^GT[GA]
3733 CACGTA
BsgI 3418 CTGCAC GTGCAG(16/14)
3497 CTGCAC
BsmBI 411 CGTCTC CGTCTC(1/5)
1787 CGTCTC
BspMI 3618 ACCTGC ACCTGC(4/8)
1126 GCAGGT
BsrBI 376 CCGCTC CCGCTC(-3/-3)
111 GAGCGG
Bst1107I 1263 GTATAC GTA^TAC
3708 GTATAC
BstYI 1188 GGATCC [GA]^GATC[CT]
1579 AGATCC
BstZ17I 1263 GTATAC GTA^TAC
3708 GTATAC
Bsu36I 2632 CCTGAGG CC^T[ACGT]AGG
3739 CCTCAGG
EarI 1718 CTCTTC CTCTTC(1/4)
2698 GAAGAG
Eco81I 2632 CCTGAGG CC^T[ACGT]AGG
3739 CCTCAGG
EcoRI 1533 GAATTC G^AATTC
1611 GAATTC
KasI 343 GGCGCC G^GCGCC
382 GGCGCC
Ksp632I 1718 CTCTTC CTCTTC(1/4)
2698 GAAGAG
KspI 32 CCGCGG CCGC^GG
572 CCGCGG
MflI 1188 GGATCC [GA]^GATC[CT]
1579 AGATCC
NaeI 99 GCCGGC GCC^GGC
3702 GCCGGC
NarI 343 GGCGCC GG^CGCC
382 GGCGCC
NgoMIV 99 GCCGGC G^CCGGC
3702 GCCGGC
PshAI 1781 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2066 GACCCAGGTC
PvuII 2174 CAGCTG CAG^CTG
2714 CAGCTG
SacII 32 CCGCGG CCGC^GG
572 CCGCGG
SfoI 343 GGCGCC GGC^GCC
382 GGCGCC
XhoII 1188 GGATCC [GA]^GATC[CT]
1579 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmaCI XmaI