Restriction map for
pF1KE3822
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AscI Asp718I AspEI
AvaI AviII BamHI BanI BbeI
BcgI BglI BsaHI BseAI BsiEI
BsiWI BsoBI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I FseI
FspI Hin1I KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NspV PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SacII SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AlwNI 1807 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 933 AGCGCT AGC^GCT
ApaI 2809 GGGCCC GGGCC^C
AspI 6654 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5652 CCTAGG C^CTAGG
BanII 2809 GGGCCC G[GA]GC[CT]^C
BbrPI 1093 CACGTG CAC^GTG
BbvCI 4260 CCTCAGC CCTCAGC(-5/-2)
BcnI 5825 CCCGG CC^[GC]GG
BlnI 5652 CCTAGG C^CTAGG
Bsp1407I 1710 TGTACA T^GTACA
BspDI 5129 ATCGAT AT^CGAT
BsrGI 1710 TGTACA T^GTACA
ClaI 5129 ATCGAT AT^CGAT
DsaI 6307 CCATGG C^C[GA][CT]GG
Eco47III 933 AGCGCT AGC^GCT
EcoNI 4260 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 3640 CCCGC CCCGC(4/6)
HpaI 633 GTTAAC GTT^AAC
NciI 5825 CCCGG CC^[GC]GG
NcoI 6307 CCATGG C^CATGG
NdeI 2899 CATATG CA^TATG
NruI 221 TCGCGA TCG^CGA
PacI 6328 TTAATTAA TTAAT^TAA
PflFI 6654 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1093 CACGTG CAC^GTG
PmlI 1093 CACGTG CAC^GTG
SalI 7325 GTCGAC G^TCGAC
SapI 4302 GCTCTTC GCTCTTC(1/4)
SexAI 3633 ACCAGGT A^CC[AT]GGT
SpeI 4665 ACTAGT A^CTAGT
SspBI 1710 TGTACA T^GTACA
Tth111I 6654 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3733 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccI 7075 GTAGAC GT^[AC][GT]AC
7325 GTCGAC
AflII 2370 CTTAAG C^TTAAG
3663 CTTAAG
Alw44I 6116 GTGCAC G^TGCAC
7168 GTGCAC
AocI 2570 CCTCAGG CC^T[ACGT]AGG
4864 CCTAAGG
ApaLI 6116 GTGCAC G^TGCAC
7168 GTGCAC
BalI 1176 TGGCCA TGG^CCA
2393 TGGCCA
BbsI 408 GAAGAC GAAGAC(2/6)
2037 GTCTTC
BfrI 2370 CTTAAG C^TTAAG
3663 CTTAAG
BpuAI 408 GAAGAC GAAGAC(2/6)
2037 GTCTTC
BsaBI 2200 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3693 GATCAGCATC
BsaI 5978 GAGACC GGTCTC(1/5)
5225 GGTCTC
BsaWI 4646 ACCGGA [AT]^CCGG[AT]
7427 ACCGGA
BsmBI 6423 GAGACG CGTCTC(1/5)
7563 GAGACG
BspLU11I 552 ACATGT A^CATGT
4934 ACATGT
BssSI 3069 CACGAG CACGAG(-5/-1)
2297 CTCGTG
BstAPI 4385 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6037 GCAAAGATTGC
Bsu36I 2570 CCTCAGG CC^T[ACGT]AGG
4864 CCTAAGG
DraIII 4456 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5992 CACAATGTG
EaeI 1176 TGGCCA [CT]^GGCC[GA]
2393 TGGCCA
Eco81I 2570 CCTCAGG CC^T[ACGT]AGG
4864 CCTAAGG
EcoRV 2534 GATATC GAT^ATC
5796 GATATC
HaeII 933 AGCGCT [GA]GCGC^[CT]
1509 GGCGCT
HgaI 4227 GACGC GACGC(5/10)
6425 GACGC
MamI 2200 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3693 GATCAGCATC
MfeI 346 CAATTG C^AATTG
4625 CAATTG
MluNI 1176 TGGCCA TGG^CCA
2393 TGGCCA
MscI 1176 TGGCCA TGG^CCA
2393 TGGCCA
MunI 346 CAATTG C^AATTG
4625 CAATTG
PflMI 108 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5334 CCAAGTTGTGG
PpuMI 300 GGGACCC [GA]G^G[AT]CC[CT]
3636 AGGTCCC
SphI 1108 GCATGC GCATG^C
1544 GCATGC
StuI 5029 AGGCCT AGG^CCT
6364 AGGCCT
Van91I 108 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5334 CCAAGTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BbvI BciVI
BclI BfaI BglII BlpI BmyI
BpmI Bpu1102I BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI