Restriction map for
pF1KE5788
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# NO CUT ENZYMES ( 127 enzymes) #
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AccI AccII AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbvCI BcgI BciVI BcnI
BglI BglII BlnI BsaAI BsaBI
BsaI BseAI BseRI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstUI
BstXI BstYI BstZ17I Cfr10I ClaI
CpoI DraII DsaI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoT22I FauI FseI FspI HgaI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1987 GGTACC G^GTACC
AciI 1459 GCGG CCGC(-3/-1)
AhdI 1374 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1084 GATCC GGATC(4/5)
AlwNI 983 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 571 CCTGAGG CC^T[ACGT]AGG
Asp718I 1987 GGTACC G^GTACC
AspEI 1374 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1600 GGCGCC GGCGC^C
BclI 851 TGATCA T^GATCA
BlpI 2194 GCTCAGC GC^T[ACGT]AGC
BpmI 1651 CTGGAG CTGGAG(16/14)
Bpu1102I 2194 GCTCAGC GC^T[ACGT]AGC
BsaWI 1665 ACCGGA [AT]^CCGG[AT]
BsmFI 596 GGGAC GGGAC(10/14)
BsrDI 475 GCAATG GCAATG(2/0)
BsrI 1650 ACTGG ACTGG(1/-1)
BssSI 233 CACGAG CACGAG(-5/-1)
BstBI 404 TTCGAA TT^CGAA
BstEII 1782 GGTAACC G^GT[ACGT]ACC
BstPI 1782 GGTAACC G^GT[ACGT]ACC
Bsu36I 571 CCTGAGG CC^T[ACGT]AGG
CelII 2194 GCTCAGC GC^T[ACGT]AGC
Cfr13I 169 GGCCC G^G[ACGT]CC
DraIII 305 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1896 GACTTTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1374 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 571 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1782 GGTAACC G^GT[ACGT]ACC
EcoRI 1678 GAATTC G^AATTC
EcoRV 2314 GATATC GAT^ATC
EcoT14I 843 CCAAGG C^C[AT][AT]GG
FbaI 851 TGATCA T^GATCA
HaeII 1600 GGCGCC [GA]GCGC^[CT]
HapII 1666 CCGG C^CGG
HpaI 553 GTTAAC GTT^AAC
HpaII 1666 CCGG C^CGG
KasI 1600 GGCGCC G^GCGCC
KpnI 1987 GGTACC GGTAC^C
MspI 1666 CCGG C^CGG
NarI 1600 GGCGCC GG^CGCC
NspV 404 TTCGAA TT^CGAA
PstI 2200 CTGCAG CTGCA^G
Sau96I 169 GGCCC G^G[ACGT]CC
ScaI 237 AGTACT AGT^ACT
SfoI 1600 GGCGCC GGC^GCC
SfuI 404 TTCGAA TT^CGAA
SphI 141 GCATGC GCATG^C
StyI 843 CCAAGG C^C[AT][AT]GG
TaqI 405 TCGA T^CGA
TthHB8I 405 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 6 GACGTC GACGT^C
1379 GACGTC
AflII 1321 CTTAAG C^TTAAG
1855 CTTAAG
AflIII 1238 ACATGT A^C[GA][CT]GT
2231 ACATGT
Asp700I 199 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAAGCTTTTC
BanI 1600 GGCGCC G^G[CT][GA]CC
1987 GGTACC
BbsI 46 GAAGAC GAAGAC(2/6)
391 GAAGAC
BfaI 340 CTAG C^TAG
1481 CTAG
BfrI 1321 CTTAAG C^TTAAG
1855 CTTAAG
BpuAI 46 GAAGAC GAAGAC(2/6)
391 GAAGAC
BsmAI 823 GAGAC GTCTC(1/5)
1633 GTCTC
BsmI 787 GAATGC GAATGC(1/-1)
1593 GAATGC
Bsp1407I 678 TGTACA T^GTACA
1329 TGTACA
BspLU11I 1238 ACATGT A^CATGT
2231 ACATGT
BsrGI 678 TGTACA T^GTACA
1329 TGTACA
CfoI 66 GCGC GCG^C
1601 GCGC
HaeIII 169 GGCC GG^CC
964 GGCC
HhaI 66 GCGC GCG^C
1601 GCGC
HinP1I 66 GCGC G^CGC
1601 GCGC
HphI 807 GGTGA GGTGA(8/7)
1966 TCACC
MaeI 340 CTAG C^TAG
1481 CTAG
MslI 247 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2328 CATATATATG
SfcI 2084 CTACAG C^T[GA][CT]AG
2200 CTGCAG
SspBI 678 TGTACA T^GTACA
1329 TGTACA
Tsp45I 808 GTGAC ^GT[GC]AC
1235 GTGAC
TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^
2000 CAGTG
XmnI 199 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAAGCTTTTC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AcyI AfaI AluI ApoI
AspHI BbvI BmyI BsaHI BsaJI
BsiHKAI Bsp1286I BssKI BstF5I BstNI
Cac8I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI Hin1I HincII
HindII HindIII HinfI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI ScrFI SfaNI SmlI
TfiI Tru9I TseI Tsp509I