Restriction map for
pF1KE6772
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BcgI BfrI BlnI BsaAI BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 73 enzymes) #
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AccII 339 CGCG CG^CG
AcsI 293 GAATTT [GA]^AATT[CT]
AcyI 381 GGCGCC G[GA]^CG[CT]C
AocI 1701 CCTCAGG CC^T[ACGT]AGG
ApaI 1458 GGGCCC GGGCC^C
ApoI 293 GAATTT [GA]^AATT[CT]
Asp700I 985 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1599 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1217 TGGCCA TGG^CCA
BamHI 459 GGATCC G^GATCC
BbeI 381 GGCGCC GGCGC^C
BbsI 1834 GAAGAC GAAGAC(2/6)
BbvCI 1041 CCTCAGC CCTCAGC(-5/-2)
BciVI 1580 GTATCC GTATCC(6/5)
BclI 1365 TGATCA T^GATCA
BglI 1422 GCCGTACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1797 AGATCT A^GATCT
BlpI 1417 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1417 GCTAAGC GC^T[ACGT]AGC
BpuAI 1834 GAAGAC GAAGAC(2/6)
BsaHI 381 GGCGCC G[GA]^CG[CT]C
BsaWI 5 TCCGGT [AT]^CCGG[AT]
BseRI 518 CTCCTC GAGGAG(10/8)
BsgI 1535 CTGCAC GTGCAG(16/14)
BsmFI 1706 GGGAC GGGAC(10/14)
BsmI 1751 GAATGC GAATGC(1/-1)
BspDI 190 ATCGAT AT^CGAT
BspHI 1830 TCATGA T^CATGA
BspMI 647 ACCTGC ACCTGC(4/8)
BsrFI 95 ACCGGC [GA]^CCGG[CT]
BstAPI 1306 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 339 CGCG CG^CG
Bsu36I 1701 CCTCAGG CC^T[ACGT]AGG
CelII 1417 GCTAAGC GC^T[ACGT]AGC
CfoI 382 GCGC GCG^C
Cfr10I 95 ACCGGC [GA]^CCGG[CT]
ClaI 190 ATCGAT AT^CGAT
CpoI 61 CGGACCG CG^G[AT]CCG
DraIII 1177 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1055 CCATGG C^C[GA][CT]GG
EaeI 1217 TGGCCA [CT]^GGCC[GA]
Eco81I 1701 CCTCAGG CC^T[ACGT]AGG
FbaI 1365 TGATCA T^GATCA
HaeII 381 GGCGCC [GA]GCGC^[CT]
HgaI 71 GCGTC GACGC(5/10)
HhaI 382 GCGC GCG^C
Hin1I 381 GGCGCC G[GA]^CG[CT]C
HinP1I 382 GCGC G^CGC
HindIII 873 AAGCTT A^AGCTT
KasI 381 GGCGCC G^GCGCC
MluNI 1217 TGGCCA TGG^CCA
MscI 1217 TGGCCA TGG^CCA
MvnI 339 CGCG CG^CG
NarI 381 GGCGCC GG^CGCC
NcoI 1055 CCATGG C^CATGG
NheI 163 GCTAGC G^CTAGC
PaeR7I 968 CTCGAG C^TCGAG
PflFI 1599 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1176 CCACATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 644 AGGACCT [GA]G^G[AT]CC[CT]
PstI 434 CTGCAG CTGCA^G
RcaI 1830 TCATGA T^CATGA
RsrII 61 CGGACCG CG^G[AT]CCG
SfcI 434 CTGCAG C^T[GA][CT]AG
SfoI 381 GGCGCC GGC^GCC
SphI 1318 GCATGC GCATG^C
Tsp45I 559 GTCAC ^GT[GC]AC
Tth111I 1599 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1176 CCACATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 135 TCTAGA T^CTAGA
XcmI 1205 CCAATGTTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 968 CTCGAG C^TCGAG
XmnI 985 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AspHI 950 GTGCTC G[AT]GC[AT]^C
1357 GAGCAC
BfaI 136 CTAG C^TAG
164 CTAG
BsiHKAI 950 GTGCTC G[AT]GC[AT]^C
1357 GAGCAC
DraII 644 AGGACCT [GA]G^G[ACGT]CC[CT]
1458 GGGCCCC
DrdI 550 GACCCAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1708 GACAAAGTGGTC
EarI 997 CTCTTC CTCTTC(1/4)
831 GAAGAG
EcoO109I 644 AGGACCT [GA]G^G[ACGT]CC[CT]
1458 GGGCCCC
FauI 25 GCGGG CCCGC(4/6)
1350 GCGGG
Ksp632I 997 CTCTTC CTCTTC(1/4)
831 GAAGAG
MaeI 136 CTAG C^TAG
164 CTAG
MaeIII 559 GTCAC ^GT[ACGT]AC
1134 GTTAC
MslI 981 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1296 CATCTGGGTG
SmlI 371 CTCAAG C^T[CT][GA]AG
968 CTCGAG
StuI 15 AGGCCT AGG^CCT
1610 AGGCCT
TaqI 191 TCGA T^CGA
969 TCGA
TfiI 575 GATTC G^A[AT]TC
990 GATTC
TthHB8I 191 TCGA T^CGA
969 TCGA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp509I TspRI XhoII