Restriction map for
pF1KE9407
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbvCI BcgI BciVI BcnI BfrI
BlnI BlpI BmyI BpmI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 589 CCGC CCGC(-3/-1)
AcsI 849 GAATTC [GA]^AATT[CT]
ApoI 849 GAATTC [GA]^AATT[CT]
AspI 862 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 494 GGACC G^G[AT]CC
BalI 250 TGGCCA TGG^CCA
BbrPI 15 CACGTG CAC^GTG
BbvI 788 GCTGC GCAGC(8/12)
BclI 428 TGATCA T^GATCA
BglI 252 GCCATTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 660 AGATCT A^GATCT
BsaAI 15 CACGTG [CT]AC^GT[GA]
BsaBI 640 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 650 CCATGG C^C[ACGT][ACGT]GG
BseRI 460 CTCCTC GAGGAG(10/8)
BsgI 704 GTGCAG GTGCAG(16/14)
BsiYI 701 CCTGTGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 701 CCTGTGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 404 TGTACA T^GTACA
BspHI 243 TCATGA T^CATGA
BspLU11I 302 ACATGT A^CATGT
BsrGI 404 TGTACA T^GTACA
BstF5I 933 CATCC GGATG(2/0)
BstXI 657 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 650 CCATGG C^C[GA][CT]GG
EaeI 250 TGGCCA [CT]^GGCC[GA]
EcoRI 849 GAATTC G^AATTC
EcoT14I 650 CCATGG C^C[AT][AT]GG
FbaI 428 TGATCA T^GATCA
Fnu4HI 788 GCTGC GC^[ACGT]GC
FokI 933 CATCC GGATG(9/13)
ItaI 788 GCTGC GC^[ACGT]GC
MaeII 16 ACGT A^CGT
MamI 640 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 250 TGGCCA TGG^CCA
MscI 250 TGGCCA TGG^CCA
MseI 782 TTAA T^TAA
NcoI 650 CCATGG C^CATGG
PflFI 862 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 15 CACGTG CAC^GTG
PmlI 15 CACGTG CAC^GTG
RcaI 243 TCATGA T^CATGA
SfiI 251 GGCCATTTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 404 TGTACA T^GTACA
SspI 813 AATATT AAT^ATT
StyI 650 CCATGG C^C[AT][AT]GG
TaqI 51 TCGA T^CGA
Tru9I 782 TTAA T^TAA
TseI 788 GCTGC G^C[AT]GC
Tsp45I 70 GTGAC ^GT[GC]AC
Tth111I 862 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 51 TCGA T^CGA
XcmI 497 CCAAATGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 224 GGATC GGATC(4/5)
510 GGATC
BbsI 618 GAAGAC GAAGAC(2/6)
899 GAAGAC
BfaI 793 CTAG C^TAG
842 CTAG
BpuAI 618 GAAGAC GAAGAC(2/6)
899 GAAGAC
BsaI 97 GGTCTC GGTCTC(1/5)
265 GGTCTC
BsrDI 186 CATTGC GCAATG(2/0)
414 CATTGC
HincII 298 GTCAAC GT[CT]^[GA]AC
529 GTCAAC
HindII 298 GTCAAC GT[CT]^[GA]AC
529 GTCAAC
HphI 765 GGTGA GGTGA(8/7)
804 GGTGA
MaeI 793 CTAG C^TAG
842 CTAG
MslI 84 CATTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
333 CATCAGTGTG
MwoI 252 GCCATTTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
413 GCATTGCTGGC
NspI 272 GCATGT [GA]CATG^[CT]
302 ACATGT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AflIII AluI BsmAI BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII HaeIII HinfI
MaeIII MboI MboII MflI MnlI
MvaI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tsp509I
TspRI XhoII