Restriction map for
pF1KB6501
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AlwI AlwNI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BbvI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HgaI HindIII HpaI KpnI
MamI MfeI MflI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
ScaI SgrAI SnaBI SpeI SphI
SspBI SspI StuI StyI SwaI
TfiI TseI Tth111I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 250 GACGTC GACGT^C
AflIII 276 ACACGT A^C[GA][CT]GT
Alw44I 187 GTGCAC G^TGCAC
AocI 100 CCTGAGG CC^T[ACGT]AGG
Aor51HI 356 AGCGCT AGC^GCT
ApaI 683 GGGCCC GGGCC^C
ApaLI 187 GTGCAC G^TGCAC
AvaII 605 GGACC G^G[AT]CC
AviII 413 TGCGCA TGC^GCA
BbsI 204 GAAGAC GAAGAC(2/6)
BfaI 631 CTAG C^TAG
BglI 19 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 432 CTCCAG CTGGAG(16/14)
BpuAI 204 GAAGAC GAAGAC(2/6)
BsaI 64 GAGACC GGTCTC(1/5)
BseRI 61 GAGGAG GAGGAG(10/8)
BsgI 487 CTGCAC GTGCAG(16/14)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsmAI 64 GAGAC GTCTC(1/5)
BsmI 242 GCATTC GAATGC(1/-1)
BsrBI 909 CCGCTC CCGCTC(-3/-3)
BssSI 835 CTCGTG CACGAG(-5/-1)
BstBI 997 TTCGAA TT^CGAA
Bsu36I 100 CCTGAGG CC^T[ACGT]AGG
DraII 683 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 190 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco47III 356 AGCGCT AGC^GCT
Eco52I 26 CGGCCG C^GGCCG
Eco81I 100 CCTGAGG CC^T[ACGT]AGG
EcoO109I 683 GGGCCCT [GA]G^G[ACGT]CC[CT]
FspI 413 TGCGCA TGC^GCA
HincII 841 GTTGAC GT[CT]^[GA]AC
HindII 841 GTTGAC GT[CT]^[GA]AC
HinfI 43 GACTC G^A[ACGT]TC
KspI 29 CCGCGG CCGC^GG
MaeI 631 CTAG C^TAG
MaeIII 977 GTGAC ^GT[ACGT]AC
MslI 369 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 29 CCGCGG C[AC]G^C[GT]G
NotI 25 GCGGCCGC GC^GGCCGC
NspI 368 ACATGC [GA]CATG^[CT]
NspV 997 TTCGAA TT^CGAA
PleI 43 GACTC GAGTC(4/5)
SacI 397 GAGCTC GAGCT^C
SacII 29 CCGCGG CCGC^GG
SbfI 135 CCTGCAGG CCTGCA^GG
SexAI 191 ACCTGGT A^CC[AT]GGT
SfiI 18 GGCCCAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 997 TTCGAA TT^CGAA
SmaI 10 CCCGGG CCC^GGG
SmlI 229 CTCAAG C^T[CT][GA]AG
Sse8387I 135 CCTGCAGG CCTGCA^GG
Tsp45I 977 GTGAC ^GT[GC]AC
TspRI 168 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 505 GTCTAC GT^[AC][GT]AC
592 GTAGAC
AcsI 764 AAATTC [GA]^AATT[CT]
1068 GAATTT
ApoI 764 AAATTC [GA]^AATT[CT]
1068 GAATTT
Asp700I 640 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
925 GAAGCTGTTC
AspHI 187 GTGCAC G[AT]GC[AT]^C
397 GAGCTC
AvaI 10 CCCGGG C^[CT]CG[GA]G
622 CCCGAG
BalI 155 TGGCCA TGG^CCA
549 TGGCCA
BanI 6 GGCGCC G^G[CT][GA]CC
78 GGCGCC
BbeI 6 GGCGCC GGCGC^C
78 GGCGCC
BsaAI 277 CACGTA [CT]AC^GT[GA]
382 TACGTG
BsiHKAI 187 GTGCAC G[AT]GC[AT]^C
397 GAGCTC
BsoBI 10 CCCGGG C^[CT]CG[GA]G
622 CCCGAG
BspMI 410 ACCTGC ACCTGC(4/8)
485 ACCTGC
BstNI 21 CCAGG CC^[AT]GG
192 CCTGG
BstXI 581 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
791 CCAGACATTTGG
DdeI 101 CTGAG C^T[ACGT]AG
1010 CTGAG
DpnII 465 GATC ^GATC
501 GATC
EarI 400 CTCTTC CTCTTC(1/4)
448 CTCTTC
EcoNI 192 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
261 CCTAAAGCAGG
EcoRII 21 CCAGG ^CC[AT]GG
192 CCTGG
KasI 6 GGCGCC G^GCGCC
78 GGCGCC
Ksp632I 400 CTCTTC CTCTTC(1/4)
448 CTCTTC
MboI 465 GATC ^GATC
501 GATC
MluNI 155 TGGCCA TGG^CCA
549 TGGCCA
MscI 155 TGGCCA TGG^CCA
549 TGGCCA
MseI 557 TTAA T^TAA
824 TTAA
MvaI 21 CCAGG CC^[AT]GG
192 CCTGG
NarI 6 GGCGCC GG^CGCC
78 GGCGCC
NdeII 465 GATC ^GATC
501 GATC
PstI 136 CTGCAG CTGCA^G
458 CTGCAG
SapI 399 GCTCTTC GCTCTTC(1/4)
447 GCTCTTC
Sau3AI 465 GATC ^GATC
501 GATC
SfoI 6 GGCGCC GGC^GCC
78 GGCGCC
Tru9I 557 TTAA T^TAA
824 TTAA
Tsp509I 765 AATT ^AATT
1069 AATT
XmnI 640 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
925 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanII BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstUI Cac8I CfoI
Cfr13I DsaI EaeI FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MboII MnlI MspI MvnI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI SfcI TaqI
TthHB8I