Restriction map for
pF1KE3275
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsiWI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraII DrdI
Eam1105I Eco47III EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI PvuII RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 1054 GGTACC G^GTACC
AccII 79 CGCG CG^CG
AccIII 116 TCCGGA T^CCGGA
AclI 535 AACGTT AA^CGTT
AflIII 390 ACACGT A^C[GA][CT]GT
AocI 241 CCTCAGG CC^T[ACGT]AGG
AseI 226 ATTAAT AT^TAAT
AsnI 226 ATTAAT AT^TAAT
Asp718I 1054 GGTACC G^GTACC
AvaII 6 GGACC G^G[AT]CC
BanI 1054 GGTACC G^G[CT][GA]CC
BbsI 295 GAAGAC GAAGAC(2/6)
BcgI 86 GCAAGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 515 CTAG C^TAG
BglII 1002 AGATCT A^GATCT
BpmI 10 CTGGAG CTGGAG(16/14)
BpuAI 295 GAAGAC GAAGAC(2/6)
BsaAI 391 CACGTA [CT]AC^GT[GA]
BsaWI 116 TCCGGA [AT]^CCGG[AT]
BseAI 116 TCCGGA T^CCGGA
BsgI 1921 GTGCAG GTGCAG(16/14)
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 1292 GAGACG CGTCTC(1/5)
BspEI 116 TCCGGA T^CCGGA
BspHI 338 TCATGA T^CATGA
BstAPI 1582 GCAGTAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 79 CGCG CG^CG
Bsu36I 241 CCTCAGG CC^T[ACGT]AGG
CfoI 80 GCGC GCG^C
DraI 202 TTTAAA TTT^AAA
DraIII 856 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
Eco81I 241 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1854 ATGCAT ATGCA^T
FauI 77 CCCGC CCCGC(4/6)
HhaI 80 GCGC GCG^C
HinP1I 80 GCGC G^CGC
KpnI 1054 GGTACC GGTAC^C
MaeI 515 CTAG C^TAG
MroI 116 TCCGGA T^CCGGA
MslI 1042 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1963 CAGCGG C[AC]G^C[GT]G
MvnI 79 CGCG CG^CG
NsiI 1854 ATGCAT ATGCA^T
PshBI 226 ATTAAT AT^TAAT
Psp1406I 535 AACGTT AA^CGTT
PstI 1084 CTGCAG CTGCA^G
RcaI 338 TCATGA T^CATGA
SacI 2020 GAGCTC GAGCT^C
SalI 314 GTCGAC G^TCGAC
SapI 54 GAAGAGC GCTCTTC(1/4)
SmaI 1418 CCCGGG CCC^GGG
SphI 1136 GCATGC GCATG^C
SspI 964 AATATT AAT^ATT
XmaCI 1418 CCCGGG C^CCGGG
XmaI 1418 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 632 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
2009 CAGGTACTG
AspHI 1345 GAGCAC G[AT]GC[AT]^C
2020 GAGCTC
AvaI 1075 CCCGAG C^[CT]CG[GA]G
1418 CCCGGG
BalI 44 TGGCCA TGG^CCA
1641 TGGCCA
BanII 106 GAGCCC G[GA]GC[CT]^C
2020 GAGCTC
BciVI 1473 GGATAC GTATCC(6/5)
113 GTATCC
BseRI 596 GAGGAG GAGGAG(10/8)
1772 GAGGAG
BsiHKAI 1345 GAGCAC G[AT]GC[AT]^C
2020 GAGCTC
BsoBI 1075 CCCGAG C^[CT]CG[GA]G
1418 CCCGGG
BsrDI 652 GCAATG GCAATG(2/0)
1896 GCAATG
BstYI 813 GGATCT [GA]^GATC[CT]
1002 AGATCT
Cfr13I 6 GGACC G^G[ACGT]CC
1959 GGCCC
DsaI 47 CCATGG C^C[GA][CT]GG
1059 CCATGG
EcoNI 1269 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2002 CCTCGGTCAGG
EcoT14I 47 CCATGG C^C[AT][AT]GG
1059 CCATGG
HgaI 1731 GCGTC GACGC(5/10)
1974 GCGTC
HincII 314 GTCGAC GT[CT]^[GA]AC
1366 GTTGAC
HindII 314 GTCGAC GT[CT]^[GA]AC
1366 GTTGAC
MflI 813 GGATCT [GA]^GATC[CT]
1002 AGATCT
MluNI 44 TGGCCA TGG^CCA
1641 TGGCCA
MscI 44 TGGCCA TGG^CCA
1641 TGGCCA
NcoI 47 CCATGG C^CATGG
1059 CCATGG
PleI 1719 GACTC GAGTC(4/5)
1783 GAGTC
Sau96I 6 GGACC G^G[ACGT]CC
1959 GGCCC
StyI 47 CCATGG C^C[AT][AT]GG
1059 CCATGG
XhoII 813 GGATCT [GA]^GATC[CT]
1002 AGATCT
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI