Restriction map for
pF1KE2370
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 485 CGCG CG^CG
ApaI 43 GGGCCC GGGCC^C
Asp700I 231 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 43 GGGCCC G[GA]GC[CT]^C
BbvI 108 GCTGC GCAGC(8/12)
BmyI 43 GGGCCC G[AGT]GC[ACT]^C
BseRI 490 GAGGAG GAGGAG(10/8)
BsgI 265 GTGCAG GTGCAG(16/14)
BsiEI 198 CGACCG CG[GA][CT]^CG
BsiYI 479 CCGTGTCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 479 CCGTGTCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 43 GGGCCC G[AGT]GC[ACT]^C
BspMI 104 ACCTGC ACCTGC(4/8)
BsrFI 59 GCCGGC [GA]^CCGG[CT]
BstUI 485 CGCG CG^CG
Cfr10I 59 GCCGGC [GA]^CCGG[CT]
DdeI 185 CTGAG C^T[ACGT]AG
DrdI 205 GACAAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 154 CTCTTC CTCTTC(1/4)
FauI 486 GCGGG CCCGC(4/6)
HaeII 305 AGCGCC [GA]GCGC^[CT]
HinfI 172 GACTC G^A[ACGT]TC
HphI 254 TCACC GGTGA(8/7)
Ksp632I 154 CTCTTC CTCTTC(1/4)
MaeIII 7 GTGAC ^GT[ACGT]AC
MslI 474 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 485 CGCG CG^CG
NaeI 59 GCCGGC GCC^GGC
NgoMIV 59 GCCGGC G^CCGGC
NlaIII 411 CATG CATG^
NspI 410 GCATGC [GA]CATG^[CT]
PleI 172 GACTC GAGTC(4/5)
PpuMI 248 AGGACCT [GA]G^G[AT]CC[CT]
SphI 410 GCATGC GCATG^C
TseI 108 GCTGC G^C[AT]GC
Tsp45I 7 GTGAC ^GT[GC]AC
XmnI 231 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 249 GGACC G^G[AT]CC
372 GGACC
BcnI 201 CCGGG CC^[GC]GG
243 CCCGG
BsmFI 203 GGGAC GGGAC(10/14)
150 GTCCC
BstYI 284 AGATCC [GA]^GATC[CT]
434 AGATCC
CfoI 33 GCGC GCG^C
306 GCGC
DsaI 288 CCACGG C^C[GA][CT]GG
318 CCACGG
Fnu4HI 108 GCTGC GC^[ACGT]GC
347 GCCGC
HhaI 33 GCGC GCG^C
306 GCGC
HinP1I 33 GCGC G^CGC
306 GCGC
ItaI 108 GCTGC GC^[ACGT]GC
347 GCCGC
MaeII 5 ACGT A^CGT
368 ACGT
MboII 155 TCTTC GAAGA(8/7)
218 TCTTC
MflI 284 AGATCC [GA]^GATC[CT]
434 AGATCC
NciI 201 CCGGG CC^[GC]GG
243 CCCGG
SfaNI 451 GATGC GCATC(5/9)
179 GCATC
XhoII 284 AGATCC [GA]^GATC[CT]
434 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AlwI BsaJI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
DraII EcoO109I EcoRII FokI HaeIII
HapII HpaII MboI MnlI MspI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI TaqI TthHB8I